AT3G56460

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004349
Physical Location & Seq
Reverse (-)
20932791 .. 20934540
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56460.1

Sequence Viewer

Length: 1047 bp
ATGGAGGCTTTGGTTTGCCGGAAATTGGGAGATCCAACTGCGACGAATCCAGGTAGTCCAGAATCTCCGGTAGAAGTAAGCAAGACTCATCCGATCCCGTCTTTGAATTCAGACACGTCGGTGAGAGTCAGAGTTATTGCTACGAGCTTGAATTACGCAAATTACCTTCAGATTCTCGGGAAATACCAGGAGAAACCTCCGTTACCTTTCATTCCGGGCTCTGATTACTCCGGAATCGTCGACGCGATTGGTCCCGCCGTCACCAAATTCCGCGTTGGAGATCGTGTCTGTTCTTTCGCCGATCTCGGTTCCTTCGCTCAGTTTATCGTCGCTGATCAATCTCGTTTGTTCCTTGTACCGGAAAGATGTGACATGGTTGCTGCAGCTGCGCTTCCTGTTGCGTTTGGGACTTCTCATGTAGCACTTGTTCATAGAGCTCGTTTAACATCTGGTCAGGTCTTATTGGTTCTAGGTGCTGCTGGTGGCGTTGGCCTTGCAGCTGTTCAAATCGGTAAGGTTTGTGGAGCCATTGTCATTGCAGTTGCTAGAGGAACTGAGAAGATTCAACTGTTGAAGTCAATGGGAGTGGATCATGTTGTTGATCTAGGCACTGAGAATGTTATTTCAAGTGTGAAAGAGTTTATCAAGACAAGAAAGCTTAAAGGAGTTGATGTTCTGTATGATCCTGTTGGAGGGAAACTAACCAAGGAGTCCATGAAAGTTCTGAAATGGGGAGCCCAGATTCTGGTGATAGGTTTCGCCAGCGGCGAAATACCTGTCATTCCTGCTAATATAGCACTTGTTAAGAACTGGACGGTTCATGGTCTTTACTGGGGTAGCTACAGGATCCATCAGCCCAATGTCCTTGAAGATTCCATCAAAGAGTTATTGTCATGGCTTTCCCGAGGATTGATCACTATTCACATCTCTCATACCTATAGCCTCTCCCAGGCCAATCTTGCATTCGGGGACCTCAAAGACAGGAAGGCAATTGGAAAAGTGATGATTGCTCTCGACCACAAGACTGCCCTGTCTTCAAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

348

Amino Acids

37.27

Weight (kDa)

9.31

Isoelectric Point (pI)

21.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 38 - 120 1.1e-13 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 162 - 286 1.2e-22 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 194 - 335 3.8e-16 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1030
AccB7I CCANNNNNTGG 1 cut(s) 745
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 245, 273
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 3 cut(s) 255, 271, 765
AclWI GGATC 6 cut(s) 26, 88, 597, 677, 841, 854
AcsI RAATTY 2 cut(s) 106, 266
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 5 cut(s) 25, 358, 692, 745, 949
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 8 cut(s) 106, 151, 506, 566, 574, 627, 869, 1038
AjiI CACGTC 1 cut(s) 117
AjnI CCWGG 3 cut(s) 49, 186, 948
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AleI CACNNNNGTG 1 cut(s) 119
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
AluBI AGCT 6 cut(s) 147, 386, 437, 500, 658, 840
AluI AGCT 6 cut(s) 147, 386, 437, 500, 658, 840
Alw21I GWGCWC 1 cut(s) 439
AlwI GGATC 6 cut(s) 26, 88, 597, 677, 841, 854
AlwNI CAGNNNCTG 1 cut(s) 745
Ama87I CYCGRG 2 cut(s) 176, 903
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 490, 951
ApeKI GCWGC 5 cut(s) 380, 383, 386, 476, 497
ApoI RAATTY 2 cut(s) 106, 266
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 251, 970
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 3 cut(s) 133, 253, 760
AvaI CYCGRG 2 cut(s) 176, 903
AvaII GGWCC 2 cut(s) 251, 970
BamHI GGATCC 1 cut(s) 846
BanII GRGCYC 3 cut(s) 221, 439, 739
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 5 cut(s) 367, 373, 395, 463, 509
BccI CCATC 2 cut(s) 858, 884
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 3 cut(s) 51, 188, 950
BclI TGATCA 2 cut(s) 334, 912
BcnI CCSGG 1 cut(s) 216
BfaI CTAG 3 cut(s) 470, 546, 605
BfmI CTRYAG 4 cut(s) 381, 841, 937, 1043
BisI GCNGC 6 cut(s) 381, 384, 387, 477, 498, 766
BlsI GCNGC 6 cut(s) 382, 385, 388, 478, 499, 767
Bme1390I CCNGG 4 cut(s) 51, 188, 216, 950
Bme18I GGWCC 2 cut(s) 251, 970
BmeT110I CYCGRG 2 cut(s) 176, 903
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 2 cut(s) 251, 970
BmiI GGNNCC 6 cut(s) 253, 310, 526, 736, 848, 971
BmrFI CCNGG 4 cut(s) 51, 188, 216, 950
BmrI ACTGGG 1 cut(s) 841
BmuI ACTGGG 1 cut(s) 841
BpiI GAAGAC 1 cut(s) 1026
BpuMI CCSGG 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 705, 904, 948
BsaWI WCCGGW 3 cut(s) 67, 230, 358
BsaXI ACNNNNNCTCC 6 cut(s) 270, 300, 516, 546, 657, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 358, 692, 745, 949
Bse1I ACTGG 2 cut(s) 815, 836
Bse3DI GCAATG 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 51, 188, 950
BseDI CCNNGG 3 cut(s) 705, 904, 948
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 5 cut(s) 25, 358, 692, 745, 949
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 3 cut(s) 332, 546, 603
BseNI ACTGG 2 cut(s) 815, 836
BseXI GCAGC 5 cut(s) 367, 373, 395, 463, 509
Bsh1236I CGCG 2 cut(s) 245, 273
BshFI GGCC 2 cut(s) 492, 953
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 2 cut(s) 176, 903
BsiSI CCGG 5 cut(s) 19, 68, 215, 231, 359
BslFI GGGAC 3 cut(s) 237, 421, 983
BslI CCNNNNNNNGG 5 cut(s) 25, 358, 692, 745, 949
BsmFI GGGAC 3 cut(s) 237, 421, 983
BsmI GAATGC 1 cut(s) 962
BsnI GGCC 2 cut(s) 492, 953
BsoBI CYCGRG 2 cut(s) 176, 903
Bsp1286I GDGCHC 3 cut(s) 221, 439, 739
Bsp13I TCCGGA 1 cut(s) 230
BspACI CCGC 3 cut(s) 255, 271, 765
BspANI GGCC 2 cut(s) 492, 953
BspCNI CTCAG 3 cut(s) 331, 547, 604
BspEI TCCGGA 1 cut(s) 230
BspFNI CGCG 2 cut(s) 245, 273
BspLI GGNNCC 6 cut(s) 253, 310, 526, 736, 848, 971
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 6 cut(s) 26, 88, 597, 677, 841, 854
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 2 cut(s) 815, 836
BssECI CCNNGG 3 cut(s) 705, 904, 948
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 3 cut(s) 51, 188, 950
Bst4CI ACNGT 2 cut(s) 570, 817
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 3 cut(s) 318, 555, 612
BstENI CCTNNNNNAGG 2 cut(s) 690, 947
BstF5I GGATG 1 cut(s) 88
BstFNI CGCG 2 cut(s) 245, 273
BstHHI GCGC 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 386, 794, 959
BstNI CCWGG 3 cut(s) 51, 188, 950
BstSCI CCNGG 4 cut(s) 49, 186, 214, 948
BstSFI CTRYAG 4 cut(s) 381, 841, 937, 1043
BstUI CGCG 2 cut(s) 245, 273
BstV1I GCAGC 5 cut(s) 367, 373, 395, 463, 509
BstV2I GAAGAC 1 cut(s) 1026
BstX2I RGATCY 2 cut(s) 31, 846
BstYI RGATCY 2 cut(s) 31, 846
BsuRI GGCC 2 cut(s) 492, 953
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 609
Cac8I GCNNGC 1 cut(s) 763
CaiI CAGNNNCTG 1 cut(s) 745
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 251, 970
CseI GACGC 1 cut(s) 251
Csp6I GTAC 1 cut(s) 356
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 6 cut(s) 373, 416, 593, 715, 821, 894
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 3 cut(s) 318, 555, 612
DrdI GACNNNNNNGTC 1 cut(s) 1030
DseDI GACNNNNNNGTC 1 cut(s) 1030
Ecl136II GAGCTC 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 705
Eco24I GRGCYC 3 cut(s) 221, 439, 739
Eco47I GGWCC 2 cut(s) 251, 970
Eco53kI GAGCTC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 2 cut(s) 176, 903
EcoICRI GAGCTC 1 cut(s) 437
EcoNI CCTNNNNNAGG 2 cut(s) 690, 947
EcoO109I RGGNCCY 1 cut(s) 970
EcoRI GAATTC 1 cut(s) 106
EcoRII CCWGG 3 cut(s) 49, 186, 948
EcoT14I CCWWGG 1 cut(s) 705
EcoT38I GRGCYC 3 cut(s) 221, 439, 739
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 6 cut(s) 376, 419, 596, 718, 824, 897
FaqI GGGAC 3 cut(s) 237, 421, 983
FatI CATG 6 cut(s) 372, 415, 592, 714, 820, 893
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 2 cut(s) 334, 912
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 6 cut(s) 381, 384, 387, 477, 498, 766
FokI GGATG 1 cut(s) 75
FriOI GRGCYC 3 cut(s) 221, 439, 739
Fsp4HI GCNGC 6 cut(s) 381, 384, 387, 477, 498, 766
FspBI CTAG 3 cut(s) 470, 546, 605
GlaI GCGC 1 cut(s) 390
GluI GCNGC 6 cut(s) 381, 384, 387, 477, 498, 766
HaeIII GGCC 2 cut(s) 492, 953
HapII CCGG 5 cut(s) 19, 68, 215, 231, 359
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 391
Hin1II CATG 6 cut(s) 376, 419, 596, 718, 824, 897
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 656
HpaII CCGG 5 cut(s) 19, 68, 215, 231, 359
HphI GGTGA 3 cut(s) 133, 253, 760
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 6 cut(s) 93, 112, 131, 171, 223, 726
Hpy188III TCNNGA 6 cut(s) 59, 178, 231, 646, 903, 1013
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 5 cut(s) 46, 121, 242, 245, 332
HpyAV CCTTC 3 cut(s) 176, 322, 979
HpyCH4III ACNGT 2 cut(s) 570, 817
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 4 cut(s) 383, 497, 539, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 794, 959
HpyF3I CTNAG 3 cut(s) 318, 555, 612
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 6 cut(s) 376, 419, 596, 718, 824, 897
HspAI GCGC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 2 cut(s) 334, 912
LmnI GCTCC 2 cut(s) 524, 734
Lsp1109I GCAGC 5 cut(s) 367, 373, 395, 463, 509
MaeI CTAG 3 cut(s) 470, 546, 605
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 3 cut(s) 201, 259, 368
MboII GAAGA 3 cut(s) 571, 881, 1026
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 2 cut(s) 31, 846
MhlI GDGCHC 3 cut(s) 221, 439, 739
MluCI AATT 6 cut(s) 23, 106, 151, 160, 266, 990
MlyI GAGTC 3 cut(s) 79, 135, 719
MmeI TCCRAC 3 cut(s) 59, 256, 670
MnlI CCTC 6 cut(s) 207, 542, 686, 899, 953, 983
MroI TCCGGA 1 cut(s) 230
MseI TTAA 3 cut(s) 443, 660, 804
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 3 cut(s) 386, 500, 765
MspI CCGG 5 cut(s) 19, 68, 215, 231, 359
MspR9I CCNGG 4 cut(s) 51, 188, 216, 950
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 1 cut(s) 962
MvaI CCWGG 3 cut(s) 51, 188, 950
MvnI CGCG 2 cut(s) 245, 273
MwoI GCNNNNNNNGC 3 cut(s) 386, 794, 959
NciI CCSGG 1 cut(s) 216
NlaIII CATG 6 cut(s) 376, 419, 596, 718, 824, 897
NlaIV GGNNCC 6 cut(s) 253, 310, 526, 736, 848, 971
NmuCI GTSAC 2 cut(s) 259, 368
OliI CACNNNNGTG 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PctI GAATGC 1 cut(s) 962
PfeI GAWTC 7 cut(s) 46, 62, 172, 234, 562, 742, 872
PflMI CCANNNNNTGG 1 cut(s) 745
PkrI GCNGC 6 cut(s) 382, 385, 388, 478, 499, 767
PleI GAGTC 3 cut(s) 79, 134, 718
PpsI GAGTC 3 cut(s) 79, 134, 718
PpuMI RGGWCCY 1 cut(s) 970
Psp124BI GAGCTC 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 3 cut(s) 49, 186, 948
PspGI CCWGG 3 cut(s) 49, 186, 948
PspN4I GGNNCC 6 cut(s) 253, 310, 526, 736, 848, 971
PspPI GGNCC 2 cut(s) 251, 970
PspPPI RGGWCCY 1 cut(s) 970
PsrI GAACNNNNNNTAC 2 cut(s) 411, 443
PstI CTGCAG 1 cut(s) 385
PstNI CAGNNNCTG 1 cut(s) 745
PsuI RGATCY 2 cut(s) 31, 846
PvuII CAGCTG 2 cut(s) 386, 500
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 439
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 3 cut(s) 443, 660, 804
SatI GCNGC 6 cut(s) 381, 384, 387, 477, 498, 766
Sau96I GGNCC 2 cut(s) 251, 970
SchI GAGTC 3 cut(s) 79, 135, 719
ScrFI CCNGG 4 cut(s) 51, 188, 216, 950
SduI GDGCHC 3 cut(s) 221, 439, 739
SfcI CTRYAG 4 cut(s) 381, 841, 937, 1043
SgrDI CGTCGACG 1 cut(s) 239
SinI GGWCC 2 cut(s) 251, 970
SmiMI CAYNNNNRTG 1 cut(s) 119
Sse9I AATT 6 cut(s) 23, 106, 151, 160, 266, 990
SsiI CCGC 3 cut(s) 255, 271, 765
SspMI CTAG 3 cut(s) 470, 546, 605
SstI GAGCTC 1 cut(s) 439
StyD4I CCNGG 4 cut(s) 49, 186, 214, 948
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 2 cut(s) 570, 817
TaiI ACGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 240, 1014
TasI AATT 6 cut(s) 23, 106, 151, 160, 266, 990
TauI GCSGC 1 cut(s) 768
TfiI GAWTC 7 cut(s) 46, 62, 172, 234, 562, 742, 872
Tru1I TTAA 3 cut(s) 443, 660, 804
Tru9I TTAA 3 cut(s) 443, 660, 804
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 2 cut(s) 259, 368
TseI GCWGC 5 cut(s) 380, 383, 386, 476, 497
Tsp45I GTSAC 2 cut(s) 259, 368
TspDTI ATGAA 4 cut(s) 199, 419, 731, 809
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 616
Van91I CCANNNNNTGG 1 cut(s) 745
VpaK11BI GGWCC 2 cut(s) 251, 970
XagI CCTNNNNNAGG 2 cut(s) 690, 947
XapI RAATTY 2 cut(s) 106, 266
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 3 cut(s) 470, 546, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.