MD00G1044900.v1.1

Quinone oxidoreductase-like protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004349
Physical Location & Seq
Forward (+)
8209422 .. 8209763
342 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1044900.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGAGGCTCTGGTGTGCCGAAAATTAGGCGACCCGACACAACCCATATCAGACAGAGGTGCAGAGAAAGCGATCATTGTGGAGAAGAACCACCCAGTTCTAAAATTAGGTTCTCCGACATCGATAAGAGTGAGAGTGAAAGCTACCAGTTTGAACTACGCCAATTATCTGCAGATACTGGGCAAGTACCAGGAGAAGCCGCCCCTCCCTTTTATCCCAGGCTTCGACTACTCCGGGACCGTCGACACCGTCGGACCCGCCGTATCCAAGTTCAAAGTTGGTGACCCTATTTGCTCTGTTGCCACCCTCAGCTCTTTCACCCAAATCATCATCGCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

114

Amino Acids

12.2

Weight (kDa)

8.95

Isoelectric Point (pI)

18.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 39 - 98 1.1e-12 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 243
AciI CCGC 2 cut(s) 200, 258
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 2 cut(s) 154, 274
AjnI CCWGG 2 cut(s) 189, 217
AluBI AGCT 2 cut(s) 143, 312
AluI AGCT 2 cut(s) 143, 312
AlwNI CAGNNNCTG 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 237, 254
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 293, 310
AvaII GGWCC 2 cut(s) 237, 254
BbvCI CCTCAGC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 2 cut(s) 191, 219
BciVI GTATCC 1 cut(s) 274
BcnI CCSGG 1 cut(s) 235
BfmI CTRYAG 1 cut(s) 170
BfuI GTATCC 1 cut(s) 274
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 3 cut(s) 191, 219, 235
Bme18I GGWCC 2 cut(s) 237, 254
BmgT120I GGNCC 2 cut(s) 237, 254
BmiI GGNNCC 2 cut(s) 238, 256
BmrFI CCNGG 3 cut(s) 191, 219, 235
BmrI ACTGGG 2 cut(s) 89, 188
BmuI ACTGGG 2 cut(s) 89, 188
Bpu10I CCTNAGC 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 235
Bsa29I ATCGAT 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 95, 147, 183
BseBI CCWGG 2 cut(s) 191, 219
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 3 cut(s) 95, 147, 183
BsgI GTGCAG 1 cut(s) 81
BshVI ATCGAT 1 cut(s) 122
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 250
Bsp143I GATC 1 cut(s) 72
BspACI CCGC 2 cut(s) 200, 258
BspCNI CTCAG 1 cut(s) 321
BspDI ATCGAT 1 cut(s) 122
BspLI GGNNCC 2 cut(s) 238, 256
BspMAI CTGCAG 1 cut(s) 174
BsrI ACTGG 3 cut(s) 95, 147, 183
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 191, 219
Bst4CI ACNGT 2 cut(s) 241, 250
BstDEI CTNAG 1 cut(s) 308
BstEII GGTNACC 1 cut(s) 281
BstKTI GATC 1 cut(s) 75
BstMBI GATC 1 cut(s) 72
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 2 cut(s) 191, 219
BstPI GGTNACC 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 189, 217, 233
BstSFI CTRYAG 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 122
BsuI GTATCC 1 cut(s) 274
BsuTUI ATCGAT 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 237, 254
ClaI ATCGAT 1 cut(s) 122
Csp6I GTAC 1 cut(s) 187
CviJI RGCY 5 cut(s) 8, 143, 199, 222, 312
CviKI_1 RGCY 5 cut(s) 8, 143, 199, 222, 312
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 1 cut(s) 308
DpnI GATC 1 cut(s) 74
DpnII GATC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 237, 254
Eco91I GGTNACC 1 cut(s) 281
EcoO65I GGTNACC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 189, 217
FaiI YATR 1 cut(s) 47
FaqI GGGAC 1 cut(s) 250
FauI CCCGC 1 cut(s) 265
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 200
GluI GCNGC 1 cut(s) 200
HapII CCGG 1 cut(s) 234
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 293, 310
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 52, 117, 254
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 2 cut(s) 245, 254
HpyCH4III ACNGT 2 cut(s) 241, 250
HpyCH4V TGCA 2 cut(s) 62, 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 308
Kzo9I GATC 1 cut(s) 72
LpnPI CCDG 8 cut(s) 108, 160, 164, 176, 203, 204, 231, 247
MaeIII GTNAC 1 cut(s) 281
MalI GATC 1 cut(s) 74
MboI GATC 1 cut(s) 72
MboII GAAGA 1 cut(s) 97
MluCI AATT 3 cut(s) 23, 104, 163
MmeI TCCRAC 2 cut(s) 140, 232
MnlI CCTC 3 cut(s) 50, 215, 317
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 3 cut(s) 191, 219, 235
MvaI CCWGG 2 cut(s) 191, 219
MwoI GCNNNNNNNGC 1 cut(s) 68
NciI CCSGG 1 cut(s) 235
NdeII GATC 1 cut(s) 72
NlaIV GGNNCC 2 cut(s) 238, 256
NmuCI GTSAC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PflFI GACNNNGTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 189, 217
PspEI GGTNACC 1 cut(s) 281
PspGI CCWGG 2 cut(s) 189, 217
PspN4I GGNNCC 2 cut(s) 238, 256
PspPI GGNCC 2 cut(s) 237, 254
PstI CTGCAG 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SalI GTCGAC 1 cut(s) 242
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 1 cut(s) 72
Sau96I GGNCC 2 cut(s) 237, 254
ScrFI CCNGG 3 cut(s) 191, 219, 235
SetI ASST 4 cut(s) 61, 112, 145, 314
SfcI CTRYAG 1 cut(s) 170
SinI GGWCC 2 cut(s) 237, 254
Sse9I AATT 3 cut(s) 23, 104, 163
SsiI CCGC 2 cut(s) 200, 258
StyD4I CCNGG 3 cut(s) 189, 217, 233
TaaI ACNGT 2 cut(s) 241, 250
TaqI TCGA 3 cut(s) 122, 225, 243
TasI AATT 3 cut(s) 23, 104, 163
TauI GCSGC 1 cut(s) 202
TseFI GTSAC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 281
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 237, 254
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.