Rorug02G0272800

Quinone oxidoreductase-like protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004349
Physical Location & Seq
Forward (+)
29139979 .. 29140296
318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0272800.1

Sequence Viewer

Length: 318 bp
ATGGAGTCGAAAAGAGAGCAAGAGAGCTATCAGAGAGAGGGAGTCAAACAGGTGGCAATGGAAACAACGGTGGAGGATGTGGTGATAGTGGGGGCAGGGATAGCCGGTTTGGCCACGGCAGTGGCGTTGAAGAGAGCAGGAATTGAAGCGCTTGTGTTGGAGAGATCCGAAGGGCTGAGAGCCACAGGTGCAGCCTTGACTCTATACCCCAACGCTTGGCGGTCTGCTCTTGATGCATTGGGAATTTCTCAGAAGCTCGCAACTCGTTATCCCCCCACGCTCAAGTACCTACATCCTCTTAGTAAACTTATTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

105

Amino Acids

11.34

Weight (kDa)

9.05

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 26 - 76 1.2e-09 FAD dependent oxidoreductase
FAD_binding_3 PF01494 26 - 87 1.7e-08 FAD binding domain
FAD_binding_2 PF00890 26 - 61 1.6e-06 FAD binding domain
Pyr_redox_3 PF13738 28 - 57 6.2e-06 Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8 PF13450 29 - 59 6.1e-07 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 216
AciI CCGC 1 cut(s) 220
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 243
AfaI GTAC 1 cut(s) 287
AfeI AGCGCT 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 2 cut(s) 130, 146
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 2 cut(s) 27, 256
AluI AGCT 2 cut(s) 27, 256
AlwI GGATC 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 150
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 243
AspLEI GCGC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 94
BalI TGGCCA 1 cut(s) 113
BbvI GCAGC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 110
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmsI GCATC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse118I RCCGGY 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 82, 292
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 2 cut(s) 167, 263
BseXI GCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 210
BshFI GGCC 1 cut(s) 113
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 216
BsnI GGCC 1 cut(s) 113
Bsp143I GATC 1 cut(s) 164
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 2 cut(s) 168, 262
BspPI GGATC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 176, 249, 299
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 2 cut(s) 82, 292
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 1 cut(s) 167
BstMBI GATC 1 cut(s) 164
BstMWI GCNNNNNNNGC 4 cut(s) 101, 110, 188, 233
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 164
BsuRI GGCC 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 2 cut(s) 82, 292
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 104
Csp6I GTAC 1 cut(s) 286
CviJI RGCY 7 cut(s) 27, 104, 113, 175, 182, 194, 256
CviKI_1 RGCY 7 cut(s) 27, 104, 113, 175, 182, 194, 256
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 3 cut(s) 176, 249, 299
DpnI GATC 1 cut(s) 166
DpnII GATC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco47III AGCGCT 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 238
FaiI YATR 2 cut(s) 205, 316
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 2 cut(s) 89, 279
Fsp4HI GCNGC 1 cut(s) 192
GlaI GCGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 1 cut(s) 113
HapII CCGG 1 cut(s) 105
HhaI GCGC 1 cut(s) 151
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 3 cut(s) 5, 42, 199
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 3 cut(s) 33, 169, 252
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 191, 236
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 110, 188, 233
HpyF3I CTNAG 3 cut(s) 176, 249, 299
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 1 cut(s) 164
LpnPI CCDG 5 cut(s) 35, 81, 118, 123, 171
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 1 cut(s) 223
MalI GATC 1 cut(s) 166
MboI GATC 1 cut(s) 164
MboII GAAGA 1 cut(s) 142
MflI RGATCY 1 cut(s) 164
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 2 cut(s) 141, 243
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 3 cut(s) 14, 51, 193
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 3 cut(s) 31, 67, 306
Mox20I TGGCCA 1 cut(s) 113
Mph1103I ATGCAT 1 cut(s) 238
MscI TGGCCA 1 cut(s) 113
MslI CAYNNNNRTG 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 113
MspI CCGG 1 cut(s) 105
MwoI GCNNNNNNNGC 4 cut(s) 101, 110, 188, 233
NdeII GATC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 238
OliI CACNNNNGTG 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 3 cut(s) 13, 50, 193
PpsI GAGTC 3 cut(s) 13, 50, 193
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 119
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 1 cut(s) 164
SchI GAGTC 3 cut(s) 14, 51, 193
SetI ASST 5 cut(s) 29, 54, 190, 258, 291
SfaNI GCATC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 2 cut(s) 141, 243
SsiI CCGC 1 cut(s) 220
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 1 cut(s) 8
TasI AATT 2 cut(s) 141, 243
TscAI CASTG 1 cut(s) 126
TseI GCWGC 1 cut(s) 191
TspRI CASTG 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 216
XapI RAATTY 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.