Rorug04G0441100

Quinone oxidoreductase-like protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000576
Physical Location & Seq
Forward (+)
59776429 .. 59782648
6220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0441100.1

Sequence Viewer

Length: 4266 bp
ATGCCGTACGACGGCGCCGTGGGCGGTTTCCGGCTCCGGTGGGACGTGTTCCTAAGCTTCAGAGGAGAAGACACCCGCCACACCATCACCAACAGCATCTACAACGCGCTCGAACGACGCGGCGTACGAGTCTTCCGCGACGACGACGGCCTCAACCGCGGCGACGACATCGCGCCGAGTCTTCTGGAAGCGATCGAGGACTCGGCGGCGGCCGTCGTCGTGTTGTCGCCGAGATACGCCGACTCGAGGTGGTGCCTTGAGGAGCTCGCGAAGATCTGCGACGGCGGCCGGAGACTGATCCTGCCGGTGTTTTACCAAGTGGACCCCTCCGACGTCCGGCGACAGAGCGGACCGTTTGAAAAGGACTTCAGAGCTCATGAAGAGCGTTTTGAAAGCAACAAGGTCTCTAAATGGAAAAACGCCATGGCCAAAGTAGGTGGCAAAGCTGGTTTTGTTGTCAACAGCAGCAACGAAGCAGAGTTGATTCAATCTCTAGTCAAAACGGTTTTGGCTGAAATAAACAAAATCCCGGTGGGTCTTGCAGCATACACAGTGGGTCTTGATTCCCGAATTGAAGACGTGATGAGGTTGTTAGATGTTAGATCAAGTGACGTTCGAGTTGTGGGGGTTCATGGCATGGGTGGTGTTGGCAAGACAACACTTGCCAAGGCTATTTTTAATAAACTTGTTGGTCATTTTGGGGGACACTGTTTCGTCTCAAATGTTAGAGAAGTCTCGGCAGGACCTAAGGGCTTGGTATCTCTTCAAAACAAACTCATCACTGATCTTTCCCCCGGTAAGGTGCCTGTGAATGACCTTGAGACTGGTATTTCTGCGATTAAGGCACTTGTATATGATAAGCGAGTTCTTCTTGTTCTGGATGATGTTGATGATGTAAACCAACTAAGTGCTTTAGTTGGCAAAGGACAATGGTTTAATGAAGGAAGCCGAATTATCATTACCACACGAGATAGAGGACTATTAGCCAGTTACCTTGTGAATTTTAAGTTGTATGAGGTCAGGGAGTTGGATGTGTCCCAGGCACTACAACTGTTTAGTTACCATGCACTGAGAAGAGATAAACCTACAGATAATTTGTTGAATCTGTCCAAGGAAATTGTATCTCTTACAGGTGGGCTACCGTTAGCTCTTGAAGTATTTGGTTCTTTTCTATGTGATAAGAGGAGAATAGAGGAATGGACAGATGCTTTGCACAAACTGACTAAGATTCGACCACAACATCTTCAGGATGTGTTGAAGATAAGTTATGATGCACTAGACGAGCAAGAGAAGTGTGTCTTCTTAGACATTGCATGCTTAATTGTAAAAATGAATGCCAAAAGAGAAGATGCAATTGACATATTGAGGAGTTGTGGTTTTGATGGTGAGATAGCAATTGCTGACCTCACAGCAAAATCACTTATTAAGATTACAGAAGACACTACATTGTGGATGCACGATCAAGTTAGAGACATGGGAAGACAAATCGTGACGCACGAAAGCCTTTTGGATCCGGGCATGCGTAGTAGACTGTGGGAACATGATAAAATCATGAATGTCTTCAAGGATGATATGGGGACAAGATGTATACAAGGCATAGTCCTAGAGGACCTAGACACTGAAAGGAAAATGAAGGGGCCAAGGGATCTAACCGGTGACAAAATTTCTTGGAACAGCTTTCAAAGTAGCCCCAATTTTACATCTGCAGTTACATACTTAAAGGAAAGATACAAAGTATATCTTCAAAATCAAGCAGAGAAAAAGAGTCGGGTTACAATTCCCTCTAAACCCTTCGGAGCTATGGTCAATCTTAGACTGCTTCAGATGAATTATGTGAATTTGGAAGGGAGCTTCGAATTTCTTCCTTCTGAATTGAAGTGGTTACAATGGAAAGGTTGTCCTTTGAAATCTCTTCCTTCTGTTCTTTTCCTTCGGCGACTTGCTGGCCTTGATCTTTCAGACAGTAGAGTGGAAAGATTGTGTAGTGGTAACAGGAATAAGGTGGCTGAGAAATTGATGTTCCTGAATCTAAGCGGCTGCAGTGTTCTCACTGCTATTCCTGATTTATCTGGGAATCATGCCTTGGAAAAGCTTATTCTTAAGAATTGCGTTGGACTGATCAAGCTCCATGATTCGATTGGGAATTTGAATACATTAGTCCACTTGAACTTGCAAGGCTGTAAAAACCTCGTTGAACTTCCAAGTGATTTCTCCGGCCTGAGAAAACTTGAGAACCTAATCCTCACCGGTTGCTCAAAATTGAAAAGACTGCCAAAAAACATCGGAAGCTTGGTATCACTAAAAGAACTTCTCCTTGAAGAAACTGCTATTGAAAGTCTTCCCGAAGCTATATTCCATCTTACAAAACTAGAAAAGCTCAGCTTGAACCGGTGCAGAGTTTTGCAAGGGCTACCTGAGGAGATTGGTAAGCTGTGTTCTTTGAAAGAAATCTCCCTTAATGAATCTGGTTTGGAAAAACTACCTGATTCCATTGGCTCTTTGGCAAACCTTGAGATACTAAGTTTGTTCTGGTGTCAGTCACTGACCACTATTCCTAACTCCCTTGGTGACCTTAATAACTTGATGGAATTTCTTACTTACGGTACTCCAATTGAAGAACTGCCGTTGAGTTTTGGTTCATTATCAAACTTGAAGGAATTATCAGTGGGACATGGTAAATTTCTGCGAACACTACCAGGTTCCATTGGAGGACTGAAATCATTAGTTGTGTTAAAGATAGATGAGACATCAATCACAGGTCTTCCACAAGAGATTAGTGCCTTGAAAACACTTGAGAAACTTGAGCTGAGGAAATGTGAATCTCTTCGATCATTACCAGAGTCAATTGGAAGCATGAAGTCACTAACTTCTATAATCATAACTGCAGCAAATATCACCGAGTTGCCTGAATCCATAGGGAGGCTGGAAAATCTTACCATGTTACAGTTGAACAGATGTAAACAGTTCTGCAAGCTTCCAGCATCAATAGGACAGTTAAAATCCTTGCACCGATTGCAGATGGTGGAAACTGCAGTGACAGAACTACCTGAAAGCTTCGGAATGCTTTCTAGCTTGATGGTTTTGAGCATGGGAAAGAAGCCTCAAAATGGGAGACATACAGAAGAGAAGTTTATACTTCCAGCTTCCTTCTCAAATCTGTCCCTGCTGTCTGAACTGGATGCTCGTGCTTGTAATATATCTGGTGAAATTCCTGATGATTTCGAGAAGTTATCATCACTTGAAACTTTGAATCTCAGCCATAATAGTTTCTGCCGCCTTCCAGCCAGCCTGAGTGGTCTGTCCACTCTCCGAGAACTTTCATTGCCTCACTGTAAGAAGCTCAAGTCTTTGCCTCCACTTCCCTCAAGTTTGGAAAAGGTAGATGTTGCAAACTGTGTTGCATTAGAAAGTATATCTGATGTTTCAAACCTGGAGAACTTGACAGATTTCAACCTTACAAATTGTGAGAAAGTGTTAGATATTCCCGGCATTGAATGCTTGGATTCCTTGACGAGGCTGTTTATGAGTGGCTGCAACAGTTGCTCATCAGCTGTCAAGAAAAGACTAGCAAAAAAGAGTTACCTGAGGAGAATACGGACTCTTAGCATTCCTGGAAGCAAGATACCTGACTGGTTTTCTCAAGATGTGATTACATTCTCAGAACGGAAAAACCATGTTCTCAAAGGGTTGATTATAGCTGTTGTAGTTTCTCTCAACCATCAAATACCGGATGACCTAAGAGAAGAACTCCCAGCTGTGGTAGATATTGTAGCAAAGATCCTCATACTGGATTCTCCTACCTTCACTTCAACGTTGGTCTTATCAGGAGTACCAAATACAAATGAAGATCAATTTTTCTTGTGCCGGTATCCAATTTCTCATCCTTTGGTTTTCCAACTGAAGGATGGTTATAAGATACATGTAGAGAGGAGAGAGCCGCCGTATGTGAAAGGAGTGGAGCTGAAGAAGTGGGGGATTCATTTGGTGTATGAAGGTGATGATGATTACGAGGAAGACGAAGAGTCATTGAAGGAAAGCCAGCAGTCCCTTTCAGAAAAACTGGCCAAGTTCTTCGGCTCTTTTGATGAAGAAGCTGACTCTACCTCCACTCATGAGCAAAATGATTTCACCTCTGAATCTAGTCATAATGAAGATGAAGGAATGGCACAGGAGATGAGAGAAAGTGACCGCCGTTACTTCATTTCTTTCTTCCTAGTGCTTTCTTTTGTCTTTCTGTTGTTGAGTTGGTTCTGGTTCTGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

1421

Amino Acids

158.96

Weight (kDa)

6.03

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 174 8.8e-42 TIR domain
TIR_2 PF13676 16 - 110 6.4e-17 TIR domain
AAA_16 PF13191 185 - 297 6.8e-06 AAA ATPase domain
NB-ARC PF00931 191 - 359 3.6e-20 NB-ARC domain
NACHT PF05729 206 - 353 3.7e-08 NACHT domain
WHD_ROQ1 PF23282 428 - 500 5.3e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 675 - 770 2.2e-06 Leucine-rich repeat region
LRR_14 PF23598 753 - 819 7.4e-10 Leucine-rich repeat region
LRR_14 PF23598 794 - 863 2.4e-07 Leucine-rich repeat region
LRR_14 PF23598 868 - 940 6.8e-08 Leucine-rich repeat region
LRR_14 PF23598 988 - 1062 1.5e-08 Leucine-rich repeat region
LRR_14 PF23598 1068 - 1214 8.3e-08 Leucine-rich repeat region
LRR_8 PF13855 1071 - 1111 6.1e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3912
AatII GACGTC 1 cut(s) 336
AccB1I GGYRCC 3 cut(s) 14, 252, 802
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 2 cut(s) 1528, 1588
AccII CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
AclI AACGTT 1 cut(s) 3812
AclWI GGATC 5 cut(s) 292, 1505, 1518, 1653, 3772
AcoI YGGCCR 4 cut(s) 210, 286, 426, 4062
AcsI RAATTY 8 cut(s) 1000, 1662, 1837, 1856, 2143, 2588, 2678, 3207
AcuI CTGAAG 6 cut(s) 43, 352, 1229, 1805, 3920, 3983
AcyI GRCGYC 2 cut(s) 15, 333
AfaI GTAC 4 cut(s) 8, 126, 2605, 3831
AflII CTTAAG 1 cut(s) 2099
AflIII ACRYGT 2 cut(s) 45, 3919
AgeI ACCGGT 3 cut(s) 1652, 2246, 2388
AhdI GACNNNNNGTC 1 cut(s) 4021
AjiI CACGTC 2 cut(s) 46, 580
AjnI CCWGG 4 cut(s) 1038, 2695, 3429, 3610
AjuI GAANNNNNNNTTGG 2 cut(s) 2066, 2098
Alw21I GWGCWC 2 cut(s) 267, 376
Alw26I GTCTC 8 cut(s) 286, 409, 721, 739, 815, 1464, 2738, 3106
AlwI GGATC 5 cut(s) 292, 1505, 1518, 1653, 3772
AlwNI CAGNNNCTG 1 cut(s) 2542
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 8 cut(s) 148, 210, 286, 426, 1637, 1945, 2215, 4062
ApeKI GCWGC 5 cut(s) 465, 542, 2037, 2885, 3531
ApoI RAATTY 8 cut(s) 1000, 1662, 1837, 1856, 2143, 2588, 2678, 3207
ArsI GACNNNNNNTTYG 2 cut(s) 1192, 1224
AsiGI ACCGGT 3 cut(s) 1652, 2246, 2388
Asp700I GAANNNNTTC 5 cut(s) 1559, 1860, 2337, 2823, 3064
AspLEI GCGC 3 cut(s) 17, 109, 175
AspS9I GGNCC 5 cut(s) 322, 350, 743, 1609, 1637
AsuC2I CCSGG 4 cut(s) 530, 795, 1515, 3486
AsuHPI GGTGA 9 cut(s) 79, 1397, 1667, 2236, 2579, 2887, 3215, 4007, 4120
AsuII TTCGAA 1 cut(s) 1854
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 4 cut(s) 322, 350, 743, 1609
AxyI CCTNAGG 3 cut(s) 747, 2415, 3584
BalI TGGCCA 2 cut(s) 428, 4064
BamHI GGATCC 1 cut(s) 1510
BanI GGYRCC 3 cut(s) 14, 252, 802
BanII GRGCYC 2 cut(s) 267, 376
BauI CACGAG 2 cut(s) 966, 3183
Bbv12I GWGCWC 2 cut(s) 267, 376
BbvCI CCTCAGC 1 cut(s) 2807
BbvI GCAGC 5 cut(s) 477, 554, 2024, 2897, 3518
BccI CCATC 8 cut(s) 92, 1376, 2364, 2578, 3013, 3070, 3726, 3899
BceAI ACGGC 8 cut(s) 2, 28, 163, 197, 298, 2608, 3925, 4176
BciT130I CCWGG 4 cut(s) 1040, 2697, 3431, 3612
BciVI GTATCC 1 cut(s) 3879
BclI TGATCA 1 cut(s) 2118
BcnI CCSGG 4 cut(s) 530, 795, 1515, 3486
BcoDI GTCTC 8 cut(s) 286, 409, 721, 739, 815, 1464, 2738, 3106
BfaI CTAG 9 cut(s) 494, 1277, 1604, 1613, 2369, 3069, 3566, 4140, 4214
BfmI CTRYAG 5 cut(s) 1086, 1704, 2038, 2883, 3030
BfoI RGCGCY 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 2099
BfuI GTATCC 1 cut(s) 3879
BglII AGATCT 1 cut(s) 273
BlpI GCTNAGC 1 cut(s) 2378
Bme1390I CCNGG 8 cut(s) 530, 795, 1040, 1515, 2697, 3431, 3486, 3612
Bme18I GGWCC 4 cut(s) 322, 350, 743, 1609
BmeRI GACNNNNNGTC 1 cut(s) 4021
BmeT110I CYCGRG 1 cut(s) 244
BmgBI CACGTC 2 cut(s) 46, 580
BmgT120I GGNCC 5 cut(s) 322, 350, 743, 1609, 1637
BmiI GGNNCC 8 cut(s) 16, 35, 254, 324, 804, 1512, 1638, 2701
BmrFI CCNGG 8 cut(s) 530, 795, 1040, 1515, 2697, 3431, 3486, 3612
BmsI GCATC 7 cut(s) 105, 1195, 1261, 1339, 1443, 2990, 3169
BplI GAGNNNNNCTC 2 cut(s) 3732, 3764
BpmI CTGGAG 1 cut(s) 3452
Bpu10I CCTNAGC 2 cut(s) 53, 2807
Bpu1102I GCTNAGC 1 cut(s) 2378
Bpu14I TTCGAA 1 cut(s) 1854
BpuEI CTTGAG 9 cut(s) 278, 839, 2249, 2531, 2813, 2822, 3326, 3349, 3624
BpuMI CCSGG 4 cut(s) 530, 795, 1515, 3486
BsaHI GRCGYC 2 cut(s) 15, 333
BsaI GGTCTC 1 cut(s) 409
BsaWI WCCGGW 5 cut(s) 36, 1652, 2246, 2388, 3727
BsaXI ACNNNNNCTCC 6 cut(s) 1360, 1390, 1788, 1818, 4088, 4118
Bse118I RCCGGY 5 cut(s) 304, 1652, 2246, 2388, 3864
Bse1I ACTGG 6 cut(s) 829, 987, 3180, 3635, 3792, 4065
Bse21I CCTNAGG 3 cut(s) 747, 2415, 3584
Bse3DI GCAATG 2 cut(s) 1308, 3320
BseBI CCWGG 4 cut(s) 1040, 2697, 3431, 3612
BseGI GGATG 9 cut(s) 886, 1036, 1255, 1458, 1573, 3184, 3736, 3880, 3910
BseMI GCAATG 2 cut(s) 1308, 3320
BseNI ACTGG 6 cut(s) 829, 987, 3180, 3635, 3792, 4065
BseRI GAGGAG 7 cut(s) 78, 275, 1198, 1381, 2432, 3601, 3943
BseX3I CGGCCG 2 cut(s) 210, 286
BseXI GCAGC 5 cut(s) 477, 554, 2024, 2897, 3518
BseYI CCCAGC 1 cut(s) 3751
BsgI GTGCAG 1 cut(s) 2413
Bsh1236I CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
Bsh1285I CGRYCG 3 cut(s) 195, 213, 289
BshFI GGCC 8 cut(s) 150, 212, 288, 428, 1639, 1947, 2217, 4064
BshNI GGYRCC 3 cut(s) 14, 252, 802
BshTI ACCGGT 3 cut(s) 1652, 2246, 2388
BsiEI CGRYCG 3 cut(s) 195, 213, 289
BsiHKAI GWGCWC 2 cut(s) 267, 376
BsiHKCI CYCGRG 1 cut(s) 244
BsiWI CGTACG 2 cut(s) 6, 124
BslFI GGGAC 7 cut(s) 56, 717, 1021, 1591, 2682, 3145, 4030
BsmAI GTCTC 8 cut(s) 286, 409, 721, 739, 815, 1464, 2738, 3106
BsmBI CGTCTC 1 cut(s) 721
BsmFI GGGAC 7 cut(s) 56, 717, 1021, 1591, 2682, 3145, 4030
BsmI GAATGC 4 cut(s) 1339, 3066, 3500, 3606
BsnI GGCC 8 cut(s) 150, 212, 288, 428, 1639, 1947, 2217, 4064
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 1854
Bsp1286I GDGCHC 2 cut(s) 267, 376
Bsp1720I GCTNAGC 1 cut(s) 2378
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 8 cut(s) 150, 212, 288, 428, 1639, 1947, 2217, 4064
BspFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BspHI TCATGA 3 cut(s) 376, 1551, 4111
BspLI GGNNCC 8 cut(s) 16, 35, 254, 324, 804, 1512, 1638, 2701
BspMAI CTGCAG 4 cut(s) 1708, 2042, 2887, 3034
BspPI GGATC 5 cut(s) 292, 1505, 1518, 1653, 3772
BspQI GCTCTTC 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 1854
BspT107I GGYRCC 3 cut(s) 14, 252, 802
BspTI CTTAAG 1 cut(s) 2099
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 2 cut(s) 1308, 3320
BsrFI RCCGGY 5 cut(s) 304, 1652, 2246, 2388, 3864
BsrI ACTGG 6 cut(s) 829, 987, 3180, 3635, 3792, 4065
BssAI RCCGGY 5 cut(s) 304, 1652, 2246, 2388, 3864
BssNAI GTATAC 1 cut(s) 1589
BssNI GRCGYC 2 cut(s) 15, 333
BssSI CACGAG 2 cut(s) 966, 3183
BssT1I CCWWGG 6 cut(s) 423, 666, 1110, 1640, 2082, 2563
Bst1107I GTATAC 1 cut(s) 1589
Bst2BI CACGAG 2 cut(s) 966, 3183
Bst2UI CCWGG 4 cut(s) 1040, 2697, 3431, 3612
Bst6I CTCTTC 7 cut(s) 375, 768, 1069, 1917, 2829, 3117, 4014
BstACI GRCGYC 2 cut(s) 15, 333
BstAFI CTTAAG 1 cut(s) 2099
BstAPI GCANNNNNTGC 3 cut(s) 3013, 3495, 3540
BstBI TTCGAA 1 cut(s) 1854
BstC8I GCNNGC 7 cut(s) 267, 1315, 1520, 1945, 2972, 3286, 4040
BstDSI CCRYGG 3 cut(s) 18, 157, 423
BstEII GGTNACC 1 cut(s) 2567
BstENI CCTNNNNNAGG 1 cut(s) 3511
BstF5I GGATG 9 cut(s) 886, 1036, 1255, 1458, 1573, 3184, 3736, 3880, 3910
BstFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 3 cut(s) 17, 109, 175
BstMAI GTCTC 8 cut(s) 286, 409, 721, 739, 815, 1464, 2738, 3106
BstMCI CGRYCG 3 cut(s) 195, 213, 289
BstMWI GCNNNNNNNGC 7 cut(s) 21, 156, 285, 842, 3013, 3495, 3540
BstNI CCWGG 4 cut(s) 1040, 2697, 3431, 3612
BstNSI RCATGY 3 cut(s) 1317, 1522, 3923
BstPI GGTNACC 1 cut(s) 2567
BstSCI CCNGG 8 cut(s) 528, 793, 1038, 1513, 2695, 3429, 3484, 3610
BstSFI CTRYAG 5 cut(s) 1086, 1704, 2038, 2883, 3030
BstUI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstV1I GCAGC 5 cut(s) 477, 554, 2024, 2897, 3518
BstX2I RGATCY 4 cut(s) 273, 1510, 1645, 3777
BstYI RGATCY 4 cut(s) 273, 1510, 1645, 3777
BstZ17I GTATAC 1 cut(s) 1589
BstZI CGGCCG 2 cut(s) 210, 286
Bsu36I CCTNAGG 3 cut(s) 747, 2415, 3584
BsuI GTATCC 1 cut(s) 3879
BsuRI GGCC 8 cut(s) 150, 212, 288, 428, 1639, 1947, 2217, 4064
BtgI CCRYGG 3 cut(s) 18, 157, 423
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 2 cut(s) 46, 580
BtsCI GGATG 9 cut(s) 886, 1036, 1255, 1458, 1573, 3184, 3736, 3880, 3910
BtsI GCAGTG 3 cut(s) 2047, 2049, 3039
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 7 cut(s) 267, 1315, 1520, 1945, 2972, 3286, 4040
CaiI CAGNNNCTG 1 cut(s) 2542
CciI TCATGA 3 cut(s) 376, 1551, 4111
CfoI GCGC 3 cut(s) 17, 109, 175
Cfr10I RCCGGY 5 cut(s) 304, 1652, 2246, 2388, 3864
Cfr13I GGNCC 5 cut(s) 322, 350, 743, 1609, 1637
Cfr42I CCGCGG 1 cut(s) 160
CpoI CGGWCCG 1 cut(s) 350
CseI GACGC 2 cut(s) 126, 1501
CsiI ACCWGGT 1 cut(s) 2695
Csp6I GTAC 4 cut(s) 7, 125, 2604, 3830
CspAI ACCGGT 3 cut(s) 1652, 2246, 2388
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CspI CGGWCCG 1 cut(s) 350
CviQI GTAC 4 cut(s) 7, 125, 2604, 3830
DinI GGCGCC 1 cut(s) 16
DriI GACNNNNNGTC 1 cut(s) 4021
EaeI YGGCCR 4 cut(s) 210, 286, 426, 4062
EagI CGGCCG 2 cut(s) 210, 286
Eam1104I CTCTTC 7 cut(s) 375, 768, 1069, 1917, 2829, 3117, 4014
Eam1105I GACNNNNNGTC 1 cut(s) 4021
EarI CTCTTC 7 cut(s) 375, 768, 1069, 1917, 2829, 3117, 4014
Ecl136II GAGCTC 2 cut(s) 265, 374
EclXI CGGCCG 2 cut(s) 210, 286
Eco130I CCWWGG 6 cut(s) 423, 666, 1110, 1640, 2082, 2563
Eco24I GRGCYC 2 cut(s) 267, 376
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 322, 350, 743, 1609
Eco52I CGGCCG 2 cut(s) 210, 286
Eco53kI GAGCTC 2 cut(s) 265, 374
Eco57I CTGAAG 6 cut(s) 43, 352, 1229, 1805, 3920, 3983
Eco81I CCTNAGG 3 cut(s) 747, 2415, 3584
Eco88I CYCGRG 1 cut(s) 244
Eco91I GGTNACC 1 cut(s) 2567
EcoICRI GAGCTC 2 cut(s) 265, 374
EcoNI CCTNNNNNAGG 1 cut(s) 3511
EcoO109I RGGNCCY 2 cut(s) 743, 1609
EcoO65I GGTNACC 1 cut(s) 2567
EcoRII CCWGG 4 cut(s) 1038, 2695, 3429, 3610
EcoT14I CCWWGG 6 cut(s) 423, 666, 1110, 1640, 2082, 2563
EcoT38I GRGCYC 2 cut(s) 267, 376
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
ErhI CCWWGG 6 cut(s) 423, 666, 1110, 1640, 2082, 2563
Esp3I CGTCTC 1 cut(s) 721
FalI AAGNNNNNCTT 6 cut(s) 1283, 1315, 1725, 1757, 2366, 2398
FaqI GGGAC 7 cut(s) 56, 717, 1021, 1591, 2682, 3145, 4030
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 1 cut(s) 2118
FblI GTMKAC 2 cut(s) 1528, 1588
FokI GGATG 9 cut(s) 893, 1043, 1262, 1465, 1580, 3191, 3743, 3867, 3917
FriOI GRGCYC 2 cut(s) 267, 376
FspBI CTAG 9 cut(s) 494, 1277, 1604, 1613, 2369, 3069, 3566, 4140, 4214
GlaI GCGC 3 cut(s) 16, 108, 174
GsaI CCCAGC 1 cut(s) 3755
GsuI CTGGAG 1 cut(s) 3452
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 8 cut(s) 150, 212, 288, 428, 1639, 1947, 2217, 4064
HgaI GACGC 2 cut(s) 126, 1501
HhaI GCGC 3 cut(s) 17, 109, 175
Hin1I GRCGYC 2 cut(s) 15, 333
Hin6I GCGC 3 cut(s) 15, 107, 173
HinP1I GCGC 3 cut(s) 15, 107, 173
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 5 cut(s) 55, 2090, 2287, 2972, 3052
HphI GGTGA 9 cut(s) 79, 1397, 1667, 2236, 2579, 2887, 3215, 4007, 4120
Hpy166II GTNNAC 8 cut(s) 322, 460, 898, 1529, 1589, 2161, 2960, 3303
Hpy8I GTNNAC 8 cut(s) 322, 460, 898, 1529, 1589, 2161, 2960, 3303
HpyCH4IV ACGT 5 cut(s) 45, 333, 579, 612, 3812
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 156, 285, 842, 3013, 3495, 3540
HpySE526I ACGT 5 cut(s) 45, 333, 579, 612, 3812
Hsp92I GRCGYC 2 cut(s) 15, 333
HspAI GCGC 3 cut(s) 15, 107, 173
KasI GGCGCC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 2118
KspI CCGCGG 1 cut(s) 160
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 6 cut(s) 39, 262, 1796, 1848, 2130, 3958
Lsp1109I GCAGC 5 cut(s) 477, 554, 2024, 2897, 3518
LweI GCATC 7 cut(s) 105, 1195, 1261, 1339, 1443, 2990, 3169
MabI ACCWGGT 1 cut(s) 2695
MaeI CTAG 9 cut(s) 494, 1277, 1604, 1613, 2369, 3069, 3566, 4140, 4214
MaeII ACGT 5 cut(s) 45, 333, 579, 612, 3812
MbiI CCGCTC 1 cut(s) 348
MfeI CAATTG 4 cut(s) 1353, 1395, 2610, 2844
MflI RGATCY 4 cut(s) 273, 1510, 1645, 3777
MhlI GDGCHC 2 cut(s) 267, 376
MlsI TGGCCA 2 cut(s) 428, 4064
MluNI TGGCCA 2 cut(s) 428, 4064
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 9 cut(s) 138, 187, 194, 236, 1774, 2849, 3592, 4031, 4091
MmeI TCCRAC 4 cut(s) 354, 1008, 2092, 3919
Mox20I TGGCCA 2 cut(s) 428, 4064
MroXI GAANNNNTTC 5 cut(s) 1559, 1860, 2337, 2823, 3064
MscI TGGCCA 2 cut(s) 428, 4064
Msp20I TGGCCA 2 cut(s) 428, 4064
MspA1I CMGCKG 3 cut(s) 159, 3551, 3755
MspCI CTTAAG 1 cut(s) 2099
MspR9I CCNGG 8 cut(s) 530, 795, 1040, 1515, 2697, 3431, 3486, 3612
MunI CAATTG 4 cut(s) 1353, 1395, 2610, 2844
Mva1269I GAATGC 4 cut(s) 1339, 3066, 3500, 3606
MvaI CCWGG 4 cut(s) 1040, 2697, 3431, 3612
MvnI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
MwoI GCNNNNNNNGC 7 cut(s) 21, 156, 285, 842, 3013, 3495, 3540
NarI GGCGCC 1 cut(s) 15
NciI CCSGG 4 cut(s) 530, 795, 1515, 3486
NcoI CCATGG 1 cut(s) 423
NlaIV GGNNCC 8 cut(s) 16, 35, 254, 324, 804, 1512, 1638, 2701
NmeAIII GCCGAG 4 cut(s) 182, 201, 255, 716
NmuCI GTSAC 8 cut(s) 608, 1489, 1655, 2538, 2567, 2859, 3034, 4184
NruI TCGCGA 1 cut(s) 269
NspI RCATGY 3 cut(s) 1317, 1522, 3923
NspV TTCGAA 1 cut(s) 1854
PaeI GCATGC 2 cut(s) 1317, 1522
PaeR7I CTCGAG 1 cut(s) 244
PagI TCATGA 3 cut(s) 376, 1551, 4111
PciI ACATGT 1 cut(s) 3919
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 4 cut(s) 15, 124, 1494, 4014
PctI GAATGC 4 cut(s) 1339, 3066, 3500, 3606
PdmI GAANNNNTTC 5 cut(s) 1559, 1860, 2337, 2823, 3064
Pfl23II CGTACG 2 cut(s) 6, 124
PfoI TCCNGGA 1 cut(s) 3610
PinAI ACCGGT 3 cut(s) 1652, 2246, 2388
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 9 cut(s) 137, 186, 194, 236, 1773, 2848, 3592, 4030, 4091
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 9 cut(s) 137, 186, 194, 236, 1773, 2848, 3592, 4030, 4091
PpuMI RGGWCCY 2 cut(s) 743, 1609
PscI ACATGT 1 cut(s) 3919
PsiI TTATAA 1 cut(s) 3912
Psp124BI GAGCTC 2 cut(s) 267, 376
Psp1406I AACGTT 1 cut(s) 3812
Psp5II RGGWCCY 2 cut(s) 743, 1609
Psp6I CCWGG 4 cut(s) 1038, 2695, 3429, 3610
PspEI GGTNACC 1 cut(s) 2567
PspFI CCCAGC 1 cut(s) 3751
PspGI CCWGG 4 cut(s) 1038, 2695, 3429, 3610
PspLI CGTACG 2 cut(s) 6, 124
PspN4I GGNNCC 8 cut(s) 16, 35, 254, 324, 804, 1512, 1638, 2701
PspPI GGNCC 5 cut(s) 322, 350, 743, 1609, 1637
PspPPI RGGWCCY 2 cut(s) 743, 1609
PspXI VCTCGAGB 1 cut(s) 244
PsrI GAACNNNNNNTAC 2 cut(s) 2419, 2451
PstI CTGCAG 4 cut(s) 1708, 2042, 2887, 3034
PstNI CAGNNNCTG 1 cut(s) 2542
PsuI RGATCY 4 cut(s) 273, 1510, 1645, 3777
PvuI CGATCG 1 cut(s) 195
PvuII CAGCTG 2 cut(s) 3551, 3755
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 4 cut(s) 8, 126, 2605, 3831
RsaNI GTAC 4 cut(s) 7, 125, 2604, 3830
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 2 cut(s) 267, 376
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 375
Sau96I GGNCC 5 cut(s) 322, 350, 743, 1609, 1637
SchI GAGTC 9 cut(s) 138, 187, 194, 236, 1774, 2849, 3592, 4031, 4091
ScrFI CCNGG 8 cut(s) 530, 795, 1040, 1515, 2697, 3431, 3486, 3612
SduI GDGCHC 2 cut(s) 267, 376
SexAI ACCWGGT 1 cut(s) 2695
SfaNI GCATC 7 cut(s) 105, 1195, 1261, 1339, 1443, 2990, 3169
SfcI CTRYAG 5 cut(s) 1086, 1704, 2038, 2883, 3030
SfoI GGCGCC 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 244
Sfr303I CCGCGG 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 1854
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 4 cut(s) 322, 350, 743, 1609
SlaI CTCGAG 1 cut(s) 244
SphI GCATGC 2 cut(s) 1317, 1522
SspDI GGCGCC 1 cut(s) 14
SspMI CTAG 9 cut(s) 494, 1277, 1604, 1613, 2369, 3069, 3566, 4140, 4214
SstI GAGCTC 2 cut(s) 267, 376
StyD4I CCNGG 8 cut(s) 528, 793, 1038, 1513, 2695, 3429, 3484, 3610
StyI CCWWGG 6 cut(s) 423, 666, 1110, 1640, 2082, 2563
TaiI ACGT 5 cut(s) 48, 336, 582, 615, 3815
TaqI TCGA 9 cut(s) 111, 195, 245, 616, 1231, 1854, 2135, 2827, 3222
TauI GCSGC 8 cut(s) 123, 162, 209, 212, 288, 2037, 3276, 3940
TseFI GTSAC 8 cut(s) 608, 1489, 1655, 2538, 2567, 2859, 3034, 4184
TseI GCWGC 5 cut(s) 465, 542, 2037, 2885, 3531
Tsp45I GTSAC 8 cut(s) 608, 1489, 1655, 2538, 2567, 2859, 3034, 4184
TspGWI ACGGA 2 cut(s) 3610, 3679
Vha464I CTTAAG 1 cut(s) 2099
VpaK11BI GGWCC 4 cut(s) 322, 350, 743, 1609
XagI CCTNNNNNAGG 1 cut(s) 3511
XapI RAATTY 8 cut(s) 1000, 1662, 1837, 1856, 2143, 2588, 2678, 3207
XceI RCATGY 3 cut(s) 1317, 1522, 3923
XcmI CCANNNNNNNNNTGG 4 cut(s) 2135, 2497, 2920, 3902
XhoI CTCGAG 1 cut(s) 244
XmiI GTMKAC 2 cut(s) 1528, 1588
XmnI GAANNNNTTC 5 cut(s) 1559, 1860, 2337, 2823, 3064
XspI CTAG 9 cut(s) 494, 1277, 1604, 1613, 2369, 3069, 3566, 4140, 4214
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.