Rh2BG545400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000576
Physical Location & Seq
Reverse (-)
75329985 .. 75335522
5538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG545400.1

Sequence Viewer

Length: 459 bp
ATGCAACTATTCGTTTCGGGCGATGTTGTTTCTGCATCTGCATTCGATAGTGATGTGACAGATGCAGCTTTGAGGCTGGGGATGGCATCTAGATGGCAATTGAGCAGCAATTCGGTCTTGGTTTGGTCAAGATCGAATCGGAAAGGTCGCTTCCGATCGAGCCCAGGGGACGTTGGTGTACCTGTTACAGGTGATGATCGTTGGCGTGTCAACGATCTTGCTATTCCCATTGGTGATGGCGTGCTATTTTGCGTGGATTTAGGGACTACTGCTGGTGGTGGTGGTGGTGCTCATGGGTATATCAGTTGCAAGGATGGAAGCAAGAGCCACTGTCAAATCAAATCGGGGCTTCTGGAGATATGGGCGAATATGTTTTTCTCCGACCTAGGTAGCTCGTTGGCGGGGCTGTTGGCGTGGCTCTGCTCATCAAAGAAGATTACCTCTGGGGATACTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

15.98

Weight (kDa)

6.05

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 401
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 188
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 2 cut(s) 68, 393
AluI AGCT 2 cut(s) 68, 393
Alw21I GWGCWC 1 cut(s) 292
ApeKI GCWGC 2 cut(s) 65, 105
AspA2I CCTAGG 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 203, 245
AvrII CCTAGG 1 cut(s) 385
BanII GRGCYC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 77, 117
BccI CCATC 4 cut(s) 76, 87, 230, 308
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 1 cut(s) 442
BfaI CTAG 3 cut(s) 90, 386, 457
BfuI GTATCC 1 cut(s) 442
BisI GCNGC 2 cut(s) 66, 106
BlnI CCTAGG 1 cut(s) 385
BlsI GCNGC 2 cut(s) 67, 107
Bme1390I CCNGG 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 44, 52, 95
BpmI CTGGAG 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 163, 164, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 188
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 163, 164, 385
BseGI GGATG 2 cut(s) 87, 319
BseLI CCNNNNNNNGG 1 cut(s) 188
BseXI GCAGC 2 cut(s) 77, 117
BseYI CCCAGC 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 292
BslFI GGGAC 2 cut(s) 182, 277
BslI CCNNNNNNNGG 1 cut(s) 188
BsmFI GGGAC 2 cut(s) 182, 277
BsmI GAATGC 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 164, 292
Bsp143I GATC 4 cut(s) 131, 155, 196, 214
BspACI CCGC 1 cut(s) 401
BssECI CCNNGG 3 cut(s) 163, 164, 385
BssMI GATC 4 cut(s) 131, 155, 196, 214
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 242
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 87, 319
BstKTI GATC 4 cut(s) 134, 158, 199, 217
BstMBI GATC 4 cut(s) 131, 155, 196, 214
BstMCI CGRYCG 1 cut(s) 158
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 77, 117
BsuI GTATCC 1 cut(s) 442
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 87, 319
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 179
CviAII CATG 1 cut(s) 293
CviJI RGCY 8 cut(s) 68, 76, 162, 327, 349, 393, 406, 418
CviKI_1 RGCY 8 cut(s) 68, 76, 162, 327, 349, 393, 406, 418
CviQI GTAC 1 cut(s) 179
DpnI GATC 4 cut(s) 133, 157, 198, 216
DpnII GATC 4 cut(s) 131, 155, 196, 214
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 1 cut(s) 296
FaiI YATR 4 cut(s) 294, 300, 361, 371
FaqI GGGAC 2 cut(s) 182, 277
FatI CATG 1 cut(s) 292
FauI CCCGC 1 cut(s) 394
Fnu4HI GCNGC 2 cut(s) 66, 106
FokI GGATG 2 cut(s) 94, 326
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 66, 106
FspBI CTAG 3 cut(s) 90, 386, 457
GluI GCNGC 2 cut(s) 66, 106
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 374
Hin1II CATG 1 cut(s) 296
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 136
HphI GGTGA 2 cut(s) 203, 245
Hpy166II GTNNAC 2 cut(s) 179, 211
Hpy188I TCNGA 3 cut(s) 141, 155, 382
Hpy188III TCNNGA 3 cut(s) 90, 129, 353
Hpy8I GTNNAC 2 cut(s) 179, 211
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 4, 35, 41, 65, 309
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 1 cut(s) 296
Kzo9I GATC 4 cut(s) 131, 155, 196, 214
LpnPI CCDG 8 cut(s) 62, 150, 174, 177, 195, 258, 338, 429
Lsp1109I GCAGC 2 cut(s) 77, 117
LweI GCATC 3 cut(s) 44, 52, 95
MaeI CTAG 3 cut(s) 90, 386, 457
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 55, 184
MalI GATC 4 cut(s) 133, 157, 198, 216
MboI GATC 4 cut(s) 131, 155, 196, 214
MboII GAAGA 1 cut(s) 445
MfeI CAATTG 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 164, 292
MluCI AATT 2 cut(s) 98, 109
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 2 cut(s) 66, 451
MslI CAYNNNNRTG 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 1 cut(s) 165
NdeII GATC 4 cut(s) 131, 155, 196, 214
NlaIII CATG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 55
PasI CCCWGGG 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PctI GAATGC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 67, 107
Ple19I CGATCG 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 163
PvuI CGATCG 1 cut(s) 158
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 91
SatI GCNGC 2 cut(s) 66, 106
Sau3AI GATC 4 cut(s) 131, 155, 196, 214
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 2 cut(s) 164, 292
SetI ASST 9 cut(s) 70, 148, 174, 184, 193, 387, 391, 395, 443
SfaNI GCATC 3 cut(s) 44, 52, 95
SmiMI CAYNNNNRTG 1 cut(s) 91
Sse9I AATT 2 cut(s) 98, 109
SsiI CCGC 1 cut(s) 401
SspMI CTAG 3 cut(s) 90, 386, 457
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 45, 134, 158
TaqII GACCGA 1 cut(s) 103
TasI AATT 2 cut(s) 98, 109
TfiI GAWTC 1 cut(s) 136
TscAI CASTG 1 cut(s) 335
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 65, 105
Tsp45I GTSAC 1 cut(s) 55
TspRI CASTG 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 186
XbaI TCTAGA 1 cut(s) 89
XmaJI CCTAGG 1 cut(s) 385
XspI CTAG 3 cut(s) 90, 386, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.