Rh2AG530800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000576
Physical Location & Seq
Reverse (-)
75932796 .. 75933452
657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG530800.1

Sequence Viewer

Length: 657 bp
ATGGAGAAAATTTACGGCTTTGGCCTCTTCATCCTTGTCTTCGTTGTTTTTGAAGGCAGTGCCTCTTTGGAGGGGAGTTTGGGCGATGTTGAGGCTGTGTTGAGTTCGGGGTTTGCAGGCGATGTTGTTTCTGCATCTGCATTTGATAGTGATGTGACAGATGCGGCTTTGAGGCTGGGGATGGCATCTAGATGGCAATTGAGCAGCGATTCGGTCTTGGTTTGGTCAAGATCGGATCGGAAAGGTTGCTTCCGATCGAGCCCACGGGACGTTGGTGTACCTGTTACAGGTGATGATCGTTGGCATGTCAACGATCTTGCTATTCCCGTTGGTGATGGAATGCTGGTTTGCGTGGATTCAGGGACTACTGGTGGTGGTAGTGGTGGTGCTCATGGGTATATCAGCTGCAAGGATGGAAGCAAGCTTCTGGAGATGTGGGCGAATCTGTTTTTCTCCGACCCAGTTAGCTCGTTGGTGGGGCTGTTGGCGTGGCTCTGCTCATCGAAGAAGATTACCTCTGGGGATACTGATTGGTGCAGCAACGAGGTCTACCACGTCGGCGATAGTGGAGGCTTGCTGGTTGGCGGCGAAGGTGTGCTGGTTTTGTGGGGTGGATCTTGTGGGGTGCCCTTAGCAATTTTTGCTTGGACCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

22.74

Weight (kDa)

4.42

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AccI GTMKAC 1 cut(s) 549
AciI CCGC 2 cut(s) 164, 585
AclWI GGATC 2 cut(s) 243, 622
AcsI RAATTY 1 cut(s) 9
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 1 cut(s) 53
AjiI CACGTC 1 cut(s) 556
AluBI AGCT 3 cut(s) 405, 424, 468
AluI AGCT 3 cut(s) 405, 424, 468
Alw21I GWGCWC 1 cut(s) 391
AlwI GGATC 2 cut(s) 243, 622
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 3 cut(s) 204, 405, 537
ApoI RAATTY 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 648
AsuHPI GGTGA 2 cut(s) 302, 344
AvaII GGWCC 1 cut(s) 648
BaeGI GKGCMC 1 cut(s) 630
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 216, 392, 549
BccI CCATC 4 cut(s) 175, 186, 329, 407
BceAI ACGGC 1 cut(s) 31
BciVI GTATCC 1 cut(s) 517
BfaI CTAG 2 cut(s) 189, 655
BfuI GTATCC 1 cut(s) 517
BisI GCNGC 5 cut(s) 165, 205, 406, 538, 586
BlsI GCNGC 5 cut(s) 166, 206, 407, 539, 587
Bme18I GGWCC 1 cut(s) 648
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 2 cut(s) 627, 650
BmrI ACTGGG 1 cut(s) 455
BmsI GCATC 3 cut(s) 143, 151, 194
BmuI ACTGGG 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 449
Bpu10I CCTNAGC 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 263
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 373, 461
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 3 cut(s) 30, 186, 418
BseLI CCNNNNNNNGG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 373, 461
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 3 cut(s) 216, 392, 549
BseYI CCCAGC 1 cut(s) 175
BsgI GTGCAG 1 cut(s) 556
Bsh1285I CGRYCG 1 cut(s) 257
BshFI GGCC 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 625
BsiEI CGRYCG 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 391
BslFI GGGAC 2 cut(s) 281, 376
BslI CCNNNNNNNGG 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 281, 376
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 263, 391, 630
Bsp143I GATC 6 cut(s) 230, 235, 254, 295, 313, 614
BspACI CCGC 2 cut(s) 164, 585
BspANI GGCC 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 627, 650
BspPI GGATC 2 cut(s) 243, 622
BspT107I GGYRCC 1 cut(s) 625
BsrI ACTGG 2 cut(s) 373, 461
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 6 cut(s) 230, 235, 254, 295, 313, 614
Bst6I CTCTTC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 118, 422, 575
BstDEI CTNAG 1 cut(s) 631
BstDSI CCRYGG 1 cut(s) 263
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 30, 186, 418
BstKTI GATC 6 cut(s) 233, 238, 257, 298, 316, 617
BstMBI GATC 6 cut(s) 230, 235, 254, 295, 313, 614
BstMCI CGRYCG 1 cut(s) 257
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 630
BstV1I GCAGC 3 cut(s) 216, 392, 549
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 1 cut(s) 24
BtgI CCRYGG 1 cut(s) 263
BtgZI GCGATG 2 cut(s) 99, 135
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 3 cut(s) 30, 186, 418
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 118, 422, 575
Cfr13I GGNCC 1 cut(s) 648
Csp6I GTAC 1 cut(s) 278
CviAII CATG 2 cut(s) 305, 392
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 1 cut(s) 631
DpnI GATC 6 cut(s) 232, 237, 256, 297, 315, 616
DpnII GATC 6 cut(s) 230, 235, 254, 295, 313, 614
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 263
FaeI CATG 2 cut(s) 308, 395
FaiI YATR 3 cut(s) 306, 393, 399
FaqI GGGAC 2 cut(s) 281, 376
FatI CATG 2 cut(s) 304, 391
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 5 cut(s) 165, 205, 406, 538, 586
FokI GGATG 3 cut(s) 17, 193, 425
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 5 cut(s) 165, 205, 406, 538, 586
FspBI CTAG 2 cut(s) 189, 655
GluI GCNGC 5 cut(s) 165, 205, 406, 538, 586
GsaI CCCAGC 1 cut(s) 179
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 1 cut(s) 24
Hin1II CATG 2 cut(s) 308, 395
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 3 cut(s) 209, 356, 442
HphI GGTGA 2 cut(s) 302, 344
Hpy166II GTNNAC 3 cut(s) 278, 310, 550
Hpy188I TCNGA 4 cut(s) 235, 240, 254, 457
Hpy188III TCNNGA 3 cut(s) 189, 228, 428
Hpy8I GTNNAC 3 cut(s) 278, 310, 550
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 2 cut(s) 47, 584
HpyCH4IV ACGT 2 cut(s) 270, 555
HpyCH4V TGCA 5 cut(s) 116, 134, 140, 408, 537
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 1 cut(s) 631
HpySE526I ACGT 2 cut(s) 270, 555
Hsp92II CATG 2 cut(s) 308, 395
Kzo9I GATC 6 cut(s) 230, 235, 254, 295, 313, 614
Lsp1109I GCAGC 3 cut(s) 216, 392, 549
LweI GCATC 3 cut(s) 143, 151, 194
MaeI CTAG 2 cut(s) 189, 655
MaeII ACGT 2 cut(s) 270, 555
MaeIII GTNAC 2 cut(s) 154, 283
MalI GATC 6 cut(s) 232, 237, 256, 297, 315, 616
MboI GATC 6 cut(s) 230, 235, 254, 295, 313, 614
MboII GAAGA 4 cut(s) 19, 31, 517, 520
MfeI CAATTG 1 cut(s) 197
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 3 cut(s) 263, 391, 630
MluCI AATT 3 cut(s) 9, 197, 636
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 8 cut(s) 35, 64, 73, 85, 165, 526, 538, 563
MslI CAYNNNNRTG 1 cut(s) 190
MspA1I CMGCKG 1 cut(s) 405
MunI CAATTG 1 cut(s) 197
Mva1269I GAATGC 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 641
NdeII GATC 6 cut(s) 230, 235, 254, 295, 313, 614
NlaIII CATG 2 cut(s) 308, 395
NlaIV GGNNCC 2 cut(s) 627, 650
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 308
PctI GAATGC 1 cut(s) 345
PfeI GAWTC 3 cut(s) 209, 356, 442
PkrI GCNGC 5 cut(s) 166, 206, 407, 539, 587
Ple19I CGATCG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 627, 650
PspPI GGNCC 1 cut(s) 648
PsuI RGATCY 1 cut(s) 614
PvuI CGATCG 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 405
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 190
SatI GCNGC 5 cut(s) 165, 205, 406, 538, 586
Sau3AI GATC 6 cut(s) 230, 235, 254, 295, 313, 614
Sau96I GGNCC 1 cut(s) 648
SduI GDGCHC 3 cut(s) 263, 391, 630
SfaNI GCATC 3 cut(s) 143, 151, 194
SinI GGWCC 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 190
Sse9I AATT 3 cut(s) 9, 197, 636
SsiI CCGC 2 cut(s) 164, 585
SspMI CTAG 2 cut(s) 189, 655
TaiI ACGT 2 cut(s) 273, 558
TaqI TCGA 2 cut(s) 257, 503
TaqII GACCGA 1 cut(s) 202
TasI AATT 3 cut(s) 9, 197, 636
TauI GCSGC 2 cut(s) 167, 588
TfiI GAWTC 3 cut(s) 209, 356, 442
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 3 cut(s) 204, 405, 537
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 1 cut(s) 19
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 648
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 1 cut(s) 9
XbaI TCTAGA 1 cut(s) 188
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 549
XspI CTAG 2 cut(s) 189, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.