Rh4AG180600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000576
Physical Location & Seq
Reverse (-)
45103937 .. 45107434
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG180600.1

Sequence Viewer

Length: 591 bp
ATGGCAGGTCTTGATCTGCTTATGTTTTTCTTGATCGCGTTGGGGAACACTGATGTAGTATTGCGTTCGGGGGTTGCATGTGCTATTGTTTCTGCGTCCAAACTTGATGGTGCAGTGAAAGATGCAGCCTCGTGGGAGGGGATGGCATCTAGATGGCAGTTGAGCAGCGATCCAGTCTTGGTTTGGTCAAGATCGGATCGGAAAGGCCGATTCCGATGGAGTCCATGGGACGATGGTGTACCTGTTCCAAGTGTTGATCTGTGGCGTGCTAGTGATCTTGCTGTCCTGGATGGTGACGGAGTGTTGGCTTGCTCGAAACTAGGGACTATTGTTTTTGATAGTGGTGCTCATGGATCTAACGATAGCAAGTATGGTGGCAAGAGCAGCTATCGAAACCAACCGGGATTGCCGGAGATGAGGACGGATCTGCTTACCCCCGGCTCGGGCACTCCAGTGGTAGGGATAATGGTATGGCTCTGCTCTTCGGAGAAGATTATCTTTGATGGTGATGCTGCCTGGTGCAGCGATGGGCTTTACCACGTAGGTGGTGGCGGTGGCTTGTTGGTTGGCGGCAACGGGAGTGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

20.58

Weight (kDa)

4.88

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 38
AciI CCGC 2 cut(s) 552, 570
AclWI GGATC 4 cut(s) 164, 204, 361, 432
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 3 cut(s) 442, 443, 458
AjnI CCWGG 2 cut(s) 285, 515
AleI CACNNNNGTG 2 cut(s) 452, 543
AluBI AGCT 1 cut(s) 387
AluI AGCT 1 cut(s) 387
Alw21I GWGCWC 1 cut(s) 349
AlwI GGATC 4 cut(s) 164, 204, 361, 432
Ama87I CYCGRG 1 cut(s) 442
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 5 cut(s) 125, 165, 384, 512, 522
AsuC2I CCSGG 2 cut(s) 402, 438
AsuHPI GGTGA 2 cut(s) 305, 518
AvaI CYCGRG 1 cut(s) 442
BaeGI GKGCMC 1 cut(s) 449
BauI CACGAG 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 5 cut(s) 137, 177, 396, 499, 534
BccI CCATC 9 cut(s) 101, 136, 147, 210, 227, 284, 497, 521, 579
BciT130I CCWGG 2 cut(s) 287, 517
BcnI CCSGG 2 cut(s) 402, 438
BfaI CTAG 3 cut(s) 150, 270, 320
BisI GCNGC 6 cut(s) 126, 166, 385, 513, 523, 571
BlsI GCNGC 6 cut(s) 127, 167, 386, 514, 524, 572
Bme1390I CCNGG 4 cut(s) 287, 402, 438, 517
BmeT110I CYCGRG 1 cut(s) 442
BmrFI CCNGG 4 cut(s) 287, 402, 438, 517
BmsI GCATC 3 cut(s) 112, 155, 499
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 2 cut(s) 402, 438
BsaAI YACGTR 1 cut(s) 541
BsaJI CCNNGG 2 cut(s) 224, 436
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 443, 458
Bse1I ACTGG 2 cut(s) 173, 452
BseBI CCWGG 2 cut(s) 287, 517
BseDI CCNNGG 2 cut(s) 224, 436
BseGI GGATG 3 cut(s) 147, 295, 590
BseLI CCNNNNNNNGG 3 cut(s) 442, 443, 458
BseNI ACTGG 2 cut(s) 173, 452
BseSI GKGCMC 1 cut(s) 449
BseXI GCAGC 5 cut(s) 137, 177, 396, 499, 534
BsgI GTGCAG 2 cut(s) 132, 541
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 3 cut(s) 401, 410, 438
BslFI GGGAC 2 cut(s) 242, 337
BslI CCNNNNNNNGG 3 cut(s) 442, 443, 458
BsmFI GGGAC 2 cut(s) 242, 337
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 442
Bsp1286I GDGCHC 2 cut(s) 349, 449
Bsp143I GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
Bsp19I CCATGG 1 cut(s) 224
BspACI CCGC 2 cut(s) 552, 570
BspANI GGCC 1 cut(s) 207
BspFNI CGCG 1 cut(s) 38
BspPI GGATC 4 cut(s) 164, 204, 361, 432
BspQI GCTCTTC 1 cut(s) 487
BsrI ACTGG 2 cut(s) 173, 452
BssECI CCNNGG 2 cut(s) 224, 436
BssMI GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 224
Bst2BI CACGAG 1 cut(s) 130
Bst2UI CCWGG 2 cut(s) 287, 517
Bst6I CTCTTC 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 541
BstC8I GCNNGC 2 cut(s) 267, 310
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 147, 295, 590
BstFNI CGCG 1 cut(s) 38
BstKTI GATC 9 cut(s) 16, 36, 172, 194, 199, 259, 277, 356, 427
BstMBI GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstNI CCWGG 2 cut(s) 287, 517
BstNSI RCATGY 1 cut(s) 81
BstSCI CCNGG 4 cut(s) 285, 400, 436, 515
BstSLI GKGCMC 1 cut(s) 449
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 5 cut(s) 137, 177, 396, 499, 534
BstX2I RGATCY 2 cut(s) 353, 424
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 2 cut(s) 353, 424
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 3 cut(s) 147, 295, 590
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 48, 120, 459
Cac8I GCNNGC 2 cut(s) 267, 310
CseI GACGC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 239
CspCI CAANNNNNGTGG 3 cut(s) 355, 390, 562
CviAII CATG 3 cut(s) 78, 225, 350
CviJI RGCY 8 cut(s) 128, 207, 308, 387, 441, 475, 532, 558
CviKI_1 RGCY 8 cut(s) 128, 207, 308, 387, 441, 475, 532, 558
CviQI GTAC 1 cut(s) 239
DpnI GATC 9 cut(s) 15, 35, 171, 193, 198, 258, 276, 355, 426
DpnII GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
Eam1104I CTCTTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 442
EcoRII CCWGG 2 cut(s) 285, 515
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 3 cut(s) 81, 228, 353
FaiI YATR 6 cut(s) 23, 79, 226, 351, 372, 472
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 2 cut(s) 242, 337
FatI CATG 3 cut(s) 77, 224, 349
Fnu4HI GCNGC 6 cut(s) 126, 166, 385, 513, 523, 571
FokI GGATG 2 cut(s) 154, 302
Fsp4HI GCNGC 6 cut(s) 126, 166, 385, 513, 523, 571
FspBI CTAG 3 cut(s) 150, 270, 320
GluI GCNGC 6 cut(s) 126, 166, 385, 513, 523, 571
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 207
HapII CCGG 3 cut(s) 401, 410, 438
HgaI GACGC 1 cut(s) 84
Hin1II CATG 3 cut(s) 81, 228, 353
HinfI GANTC 2 cut(s) 210, 220
HpaII CCGG 3 cut(s) 401, 410, 438
HphI GGTGA 2 cut(s) 305, 518
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 4 cut(s) 196, 201, 215, 487
Hpy188III TCNNGA 4 cut(s) 11, 31, 150, 189
Hpy8I GTNNAC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 4 cut(s) 77, 113, 125, 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 3 cut(s) 81, 228, 353
Kzo9I GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
LguI GCTCTTC 1 cut(s) 487
Lsp1109I GCAGC 5 cut(s) 137, 177, 396, 499, 534
LweI GCATC 3 cut(s) 112, 155, 499
MaeI CTAG 3 cut(s) 150, 270, 320
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 1 cut(s) 293
MalI GATC 9 cut(s) 15, 35, 171, 193, 198, 258, 276, 355, 426
MboI GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
MboII GAAGA 2 cut(s) 474, 502
MflI RGATCY 2 cut(s) 353, 424
MhlI GDGCHC 2 cut(s) 349, 449
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 3 cut(s) 130, 139, 411
MslI CAYNNNNRTG 3 cut(s) 151, 452, 543
MspI CCGG 3 cut(s) 401, 410, 438
MspR9I CCNGG 4 cut(s) 287, 402, 438, 517
MvaI CCWGG 2 cut(s) 287, 517
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 384
NciI CCSGG 2 cut(s) 402, 438
NcoI CCATGG 1 cut(s) 224
NdeII GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
NlaIII CATG 3 cut(s) 81, 228, 353
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 81
OliI CACNNNNGTG 2 cut(s) 452, 543
PciSI GCTCTTC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 205
PfeI GAWTC 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 6 cut(s) 127, 167, 386, 514, 524, 572
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
Ppu21I YACGTR 1 cut(s) 541
Psp6I CCWGG 2 cut(s) 285, 515
PspGI CCWGG 2 cut(s) 285, 515
PsuI RGATCY 2 cut(s) 353, 424
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
RseI CAYNNNNRTG 3 cut(s) 151, 452, 543
SapI GCTCTTC 1 cut(s) 487
SatI GCNGC 6 cut(s) 126, 166, 385, 513, 523, 571
Sau3AI GATC 9 cut(s) 13, 33, 169, 191, 196, 256, 274, 353, 424
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 4 cut(s) 287, 402, 438, 517
SduI GDGCHC 2 cut(s) 349, 449
SetI ASST 5 cut(s) 10, 244, 389, 543, 547
SfaNI GCATC 3 cut(s) 112, 155, 499
SmiMI CAYNNNNRTG 3 cut(s) 151, 452, 543
SsiI CCGC 2 cut(s) 552, 570
SspMI CTAG 3 cut(s) 150, 270, 320
StyD4I CCNGG 4 cut(s) 285, 400, 436, 515
StyI CCWWGG 1 cut(s) 224
TaiI ACGT 1 cut(s) 543
TaqI TCGA 2 cut(s) 314, 391
TauI GCSGC 1 cut(s) 573
TfiI GAWTC 1 cut(s) 210
TscAI CASTG 3 cut(s) 55, 120, 459
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 5 cut(s) 125, 165, 384, 512, 522
Tsp45I GTSAC 1 cut(s) 293
TspGWI ACGGA 2 cut(s) 312, 437
TspRI CASTG 3 cut(s) 55, 120, 459
XbaI TCTAGA 1 cut(s) 149
XceI RCATGY 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 2 cut(s) 180, 545
XspI CTAG 3 cut(s) 150, 270, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.