Rh2AG530900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000576
Physical Location & Seq
Reverse (-)
75935208 .. 75935447
240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG530900.1

Sequence Viewer

Length: 240 bp
ATGGCGGCGGCCATAGATGAGTTTAGGGCTGTGTATGTGGACGGTTTCACCAACTCTGACATGGTAATCGTCGCTCTGATCATTCTGAAAAAAGGTTTTGATAGACATGCGGTGGACGGGATCCGCAATACGTATGAAGATTTTTATTGCTCCTTGAACTGCCGGAGCTCGAGGAGGGGGAATCGTACCTACTGTTGTCCTTCATCCTCGAGGTTGGTCAGTTCTAATGAAGAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

79

Amino Acids

8.96

Weight (kDa)

7.66

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 208
AciI CCGC 4 cut(s) 5, 8, 110, 124
AclI AACGTT 1 cut(s) 235
AclWI GGATC 2 cut(s) 115, 128
AcoI YGGCCR 1 cut(s) 9
AfaI GTAC 1 cut(s) 187
AgsI TTSAA 1 cut(s) 157
AluBI AGCT 1 cut(s) 168
AluI AGCT 1 cut(s) 168
Alw21I GWGCWC 1 cut(s) 170
AlwI GGATC 2 cut(s) 115, 128
Ama87I CYCGRG 2 cut(s) 169, 208
AoxI GGCC 1 cut(s) 9
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 2 cut(s) 169, 208
BamHI GGATCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 170
BclI TGATCA 1 cut(s) 78
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
BmeT110I CYCGRG 2 cut(s) 169, 208
BmiI GGNNCC 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 132
BseGI GGATG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 187
BshFI GGCC 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 170
BsiHKCI CYCGRG 2 cut(s) 169, 208
BsiSI CCGG 1 cut(s) 163
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 2 cut(s) 169, 208
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 78, 120
BspACI CCGC 4 cut(s) 5, 8, 110, 124
BspANI GGCC 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 122
BspPI GGATC 2 cut(s) 115, 128
BssMI GATC 2 cut(s) 78, 120
Bst4CI ACNGT 2 cut(s) 44, 194
BstBAI YACGTR 1 cut(s) 132
BstF5I GGATG 1 cut(s) 203
BstKTI GATC 2 cut(s) 81, 123
BstMBI GATC 2 cut(s) 78, 120
BstNSI RCATGY 1 cut(s) 110
BstSNI TACGTA 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 203
Csp6I GTAC 1 cut(s) 186
CviAII CATG 2 cut(s) 61, 107
CviJI RGCY 3 cut(s) 11, 29, 168
CviKI_1 RGCY 3 cut(s) 11, 29, 168
CviQI GTAC 1 cut(s) 186
DpnI GATC 2 cut(s) 80, 122
DpnII GATC 2 cut(s) 78, 120
EaeI YGGCCR 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 168
Eco105I TACGTA 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 168
Eco88I CYCGRG 2 cut(s) 169, 208
EcoICRI GAGCTC 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 170
FaeI CATG 2 cut(s) 64, 110
FaiI YATR 5 cut(s) 14, 36, 62, 108, 135
FatI CATG 2 cut(s) 60, 106
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 6, 9
FokI GGATG 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 6, 9
GluI GCNGC 2 cut(s) 6, 9
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 163
Hin1II CATG 2 cut(s) 64, 110
HinfI GANTC 1 cut(s) 181
HpaII CCGG 1 cut(s) 163
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 2 cut(s) 40, 115
Hpy188I TCNGA 3 cut(s) 58, 78, 87
Hpy8I GTNNAC 2 cut(s) 40, 115
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 210
HpyCH4III ACNGT 2 cut(s) 44, 194
HpyCH4IV ACGT 2 cut(s) 131, 235
HpySE526I ACGT 2 cut(s) 131, 235
Hsp92II CATG 2 cut(s) 64, 110
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 2 cut(s) 78, 120
LmnI GCTCC 2 cut(s) 155, 165
LpnPI CCDG 1 cut(s) 176
MaeII ACGT 2 cut(s) 131, 235
MalI GATC 2 cut(s) 80, 122
MboI GATC 2 cut(s) 78, 120
MboII GAAGA 1 cut(s) 149
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 170
MnlI CCTC 4 cut(s) 165, 168, 204, 217
MspI CCGG 1 cut(s) 163
NdeII GATC 2 cut(s) 78, 120
NlaIII CATG 2 cut(s) 64, 110
NlaIV GGNNCC 1 cut(s) 122
NspI RCATGY 1 cut(s) 110
PaeR7I CTCGAG 2 cut(s) 169, 208
PfeI GAWTC 1 cut(s) 181
PkrI GCNGC 2 cut(s) 7, 10
Ppu21I YACGTR 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 170
Psp1406I AACGTT 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 122
PspXI VCTCGAGB 2 cut(s) 169, 208
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SacI GAGCTC 1 cut(s) 170
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 2 cut(s) 78, 120
SduI GDGCHC 1 cut(s) 170
SetI ASST 6 cut(s) 97, 134, 170, 191, 215, 238
Sfr274I CTCGAG 2 cut(s) 169, 208
SgeI CNNG 9 cut(s) 73, 119, 130, 166, 175, 181, 183, 220, 222
SlaI CTCGAG 2 cut(s) 169, 208
SmlI CTYRAG 2 cut(s) 169, 208
SmoI CTYRAG 2 cut(s) 169, 208
SnaBI TACGTA 1 cut(s) 132
SsiI CCGC 4 cut(s) 5, 8, 110, 124
SstI GAGCTC 1 cut(s) 170
TaaI ACNGT 2 cut(s) 44, 194
TaiI ACGT 2 cut(s) 134, 238
TaqI TCGA 2 cut(s) 170, 209
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 1 cut(s) 181
TspDTI ATGAA 2 cut(s) 150, 192
XceI RCATGY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XhoI CTCGAG 2 cut(s) 169, 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.