Rh1AG357100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000576
Physical Location & Seq
Reverse (-)
59263154 .. 59263681
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG357100.1

Sequence Viewer

Length: 528 bp
ATGGTGCACCTACTACAAGTGTTGATATTTGGCGTGCCGCCATCGTGGAAGGTGTACTGCTTTGCTCAGACTCGGGGACTGCTGCTTTTGATGGCGGTGCTCTTGGGTATAATGGCTGCAAGTGTGGAGCAGTGGAGGCAAGGGCGGCTATCAATTGTATCGACTACCGGAGGTAAGGATGGCCCTGCTTTCCTCGGGGCCGGACCCTTCGTTGGTGGGGATGACAGCTTGGTTCTACTTGACAGTGGCTGGTACAGCGGCAGGATCTACCACATCAGTGGCAGCGGCAGCGACTTGCAAATCAGAGACGATGATGGGCTTGAACAGGGGGGTGGATTCTATGGGGTGTCCTTAGCAATCTTTTCTTGGGCCCTCTGGGCTTCATTCTGGTTTGTGAGCATTACTTGCTGGGCTTGGATGGGCTGGGCCCTCAGCTTTAAGGGCTGGGCCCTCAGCTTTAAGGGCTGGGCTGACTTTGTCAAGGGAGTGGAAGGTGGTTTGTCATCTTTTGCTCCGATTATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

18.91

Weight (kDa)

5.52

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 38, 95, 145, 258, 285
AclWI GGATC 1 cut(s) 272
AfaI GTAC 2 cut(s) 56, 254
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 1 cut(s) 323
AleI CACNNNNGTG 1 cut(s) 276
AluBI AGCT 3 cut(s) 228, 435, 456
AluI AGCT 3 cut(s) 228, 435, 456
Alw21I GWGCWC 2 cut(s) 9, 102
Alw26I GTCTC 1 cut(s) 300
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 1 cut(s) 272
AlwNI CAGNNNCTG 1 cut(s) 249
Ama87I CYCGRG 2 cut(s) 72, 194
AoxI GGCC 5 cut(s) 181, 198, 369, 426, 447
ApaI GGGCCC 3 cut(s) 373, 430, 451
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 4 cut(s) 82, 116, 282, 288
AspS9I GGNCC 9 cut(s) 182, 198, 203, 369, 370, 426, 427, 447, 448
AvaI CYCGRG 2 cut(s) 72, 194
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 4 cut(s) 9, 373, 430, 451
BanII GRGCYC 3 cut(s) 373, 430, 451
Bbv12I GWGCWC 2 cut(s) 9, 102
BbvCI CCTCAGC 2 cut(s) 431, 452
BbvI GCAGC 4 cut(s) 69, 103, 294, 300
BccI CCATC 5 cut(s) 49, 85, 173, 308, 412
BcoDI GTCTC 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 377
BisI GCNGC 8 cut(s) 38, 83, 117, 146, 259, 283, 286, 289
BlsI GCNGC 8 cut(s) 39, 84, 118, 147, 260, 284, 287, 290
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 2 cut(s) 72, 194
BmgT120I GGNCC 9 cut(s) 182, 198, 203, 369, 370, 426, 427, 447, 448
BmiI GGNNCC 5 cut(s) 199, 205, 371, 428, 449
Bpu10I CCTNAGC 3 cut(s) 352, 431, 452
BsaJI CCNNGG 1 cut(s) 193
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 3 cut(s) 184, 226, 423
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 3 cut(s) 80, 445, 466
BseSI GKGCMC 4 cut(s) 9, 373, 430, 451
BseXI GCAGC 4 cut(s) 69, 103, 294, 300
BseYI CCCAGC 4 cut(s) 408, 423, 444, 465
BshFI GGCC 5 cut(s) 183, 200, 371, 428, 449
BsiHKAI GWGCWC 2 cut(s) 9, 102
BsiHKCI CYCGRG 2 cut(s) 72, 194
BsiSI CCGG 2 cut(s) 168, 201
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 300
BsmBI CGTCTC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 5 cut(s) 183, 200, 371, 428, 449
BsoBI CYCGRG 2 cut(s) 72, 194
Bsp120I GGGCCC 3 cut(s) 369, 426, 447
Bsp1286I GDGCHC 5 cut(s) 9, 102, 373, 430, 451
Bsp143I GATC 1 cut(s) 264
BspACI CCGC 5 cut(s) 38, 95, 145, 258, 285
BspANI GGCC 5 cut(s) 183, 200, 371, 428, 449
BspCNI CTCAG 3 cut(s) 79, 444, 465
BspLI GGNNCC 5 cut(s) 199, 205, 371, 428, 449
BspPI GGATC 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 245
BstAPI GCANNNNNTGC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 4 cut(s) 66, 352, 431, 452
BstF5I GGATG 3 cut(s) 184, 226, 423
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 1 cut(s) 264
BstMWI GCNNNNNNNGC 8 cut(s) 136, 145, 255, 288, 377, 405, 441, 462
BstSLI GKGCMC 4 cut(s) 9, 373, 430, 451
BstV1I GCAGC 4 cut(s) 69, 103, 294, 300
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 278
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 5 cut(s) 183, 200, 371, 428, 449
BtsCI GGATG 3 cut(s) 184, 226, 423
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 3 cut(s) 137, 250, 283
Cac8I GCNNGC 1 cut(s) 35
CaiI CAGNNNCTG 1 cut(s) 249
Cfr13I GGNCC 9 cut(s) 182, 198, 203, 369, 370, 426, 427, 447, 448
Csp6I GTAC 2 cut(s) 55, 253
CviQI GTAC 2 cut(s) 55, 253
DdeI CTNAG 4 cut(s) 66, 352, 431, 452
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
Eco24I GRGCYC 3 cut(s) 373, 430, 451
Eco47I GGWCC 1 cut(s) 203
Eco88I CYCGRG 2 cut(s) 72, 194
EcoO109I RGGNCCY 3 cut(s) 370, 427, 448
EcoT38I GRGCYC 3 cut(s) 373, 430, 451
Esp3I CGTCTC 1 cut(s) 300
FaiI YATR 2 cut(s) 110, 342
FaqI GGGAC 1 cut(s) 90
Fnu4HI GCNGC 8 cut(s) 38, 83, 117, 146, 259, 283, 286, 289
FokI GGATG 3 cut(s) 191, 233, 430
FriOI GRGCYC 3 cut(s) 373, 430, 451
Fsp4HI GCNGC 8 cut(s) 38, 83, 117, 146, 259, 283, 286, 289
GluI GCNGC 8 cut(s) 38, 83, 117, 146, 259, 283, 286, 289
GsaI CCCAGC 4 cut(s) 412, 427, 448, 469
HaeIII GGCC 5 cut(s) 183, 200, 371, 428, 449
HapII CCGG 2 cut(s) 168, 201
HinfI GANTC 2 cut(s) 70, 336
HpaII CCGG 2 cut(s) 168, 201
Hpy166II GTNNAC 2 cut(s) 7, 55
Hpy188I TCNGA 3 cut(s) 69, 305, 516
Hpy8I GTNNAC 2 cut(s) 7, 55
HpyAV CCTTC 3 cut(s) 43, 217, 485
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4V TGCA 3 cut(s) 7, 119, 298
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 145, 255, 288, 377, 405, 441, 462
HpyF3I CTNAG 4 cut(s) 66, 352, 431, 452
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 127, 517
Lsp1109I GCAGC 4 cut(s) 69, 103, 294, 300
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 5 cut(s) 9, 102, 373, 430, 451
MluCI AATT 1 cut(s) 153
MlyI GAGTC 1 cut(s) 64
MnlI CCTC 6 cut(s) 129, 164, 203, 383, 440, 461
MseI TTAA 3 cut(s) 438, 459, 526
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 2 cut(s) 258, 285
MspI CCGG 2 cut(s) 168, 201
MunI CAATTG 1 cut(s) 153
MwoI GCNNNNNNNGC 8 cut(s) 136, 145, 255, 288, 377, 405, 441, 462
NdeII GATC 1 cut(s) 264
NlaIV GGNNCC 5 cut(s) 199, 205, 371, 428, 449
OliI CACNNNNGTG 1 cut(s) 276
PfeI GAWTC 1 cut(s) 336
PflFI GACNNNGTC 1 cut(s) 476
PkrI GCNGC 8 cut(s) 39, 84, 118, 147, 260, 284, 287, 290
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PspFI CCCAGC 4 cut(s) 408, 423, 444, 465
PspN4I GGNNCC 5 cut(s) 199, 205, 371, 428, 449
PspOMI GGGCCC 3 cut(s) 369, 426, 447
PspPI GGNCC 9 cut(s) 182, 198, 203, 369, 370, 426, 427, 447, 448
PstNI CAGNNNCTG 1 cut(s) 249
PsuI RGATCY 1 cut(s) 264
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 2 cut(s) 56, 254
RsaNI GTAC 2 cut(s) 55, 253
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 3 cut(s) 438, 459, 526
SatI GCNGC 8 cut(s) 38, 83, 117, 146, 259, 283, 286, 289
Sau3AI GATC 1 cut(s) 264
Sau96I GGNCC 9 cut(s) 182, 198, 203, 369, 370, 426, 427, 447, 448
SchI GAGTC 1 cut(s) 64
SduI GDGCHC 5 cut(s) 9, 102, 373, 430, 451
SetI ASST 7 cut(s) 12, 54, 175, 230, 437, 458, 496
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 1 cut(s) 153
SsiI CCGC 5 cut(s) 38, 95, 145, 258, 285
TaaI ACNGT 1 cut(s) 245
TaqI TCGA 1 cut(s) 161
TasI AATT 1 cut(s) 153
TatI WGTACW 1 cut(s) 54
TauI GCSGC 4 cut(s) 40, 148, 261, 288
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 3 cut(s) 438, 459, 526
Tru9I TTAA 3 cut(s) 438, 459, 526
TscAI CASTG 3 cut(s) 137, 250, 283
TseI GCWGC 4 cut(s) 82, 116, 282, 288
TspDTI ATGAA 1 cut(s) 372
TspRI CASTG 3 cut(s) 137, 250, 283
Tth111I GACNNNGTC 1 cut(s) 476
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.