Rh5DG114200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000576
Physical Location & Seq
Forward (+)
10353534 .. 10353950
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG114200.1

Sequence Viewer

Length: 417 bp
ATGGCAGATTTTGGTCTGCTTATTCTTTTCTTGATTGCTGTGGGGAATACTGAGGCGGTCTTGAGTTCGGGGCATGGAGGCATTGTTGTCTCTACGTTCATGCTAGATGGTGATATGAATGATGAGGCCTCGTGGGAGGGGATGACATCTAGATGGCAGTTGAGCAGTGATCCGGTCTTGTTTGGTCAAGATCGGATCGGAAAGGTCGATTCCAATCGAGTCCATGGAATGACTGTGTACCTGTTACAGGGGTTGATCCTTGGAGGGCCGGAGATCTTTCTATCCCGGATGGTGACGGAGTGCTGGTTTGCTCGAACCCAAGGGCTATTGCTTCTTATGAGTGTGCTTTTGGGGATAACATCTGCAAGTATTGCGGCAAGTGCGGTGGTCGAAACGAATCAGGGTTTCTGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.83

Weight (kDa)

4.46

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 56, 374, 383
AclWI GGATC 3 cut(s) 164, 203, 250
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 3 cut(s) 164, 203, 250
AoxI GGCC 2 cut(s) 126, 266
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 122, 304
BauI CACGAG 1 cut(s) 130
BccI CCATC 3 cut(s) 101, 147, 283
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 2 cut(s) 104, 150
BglII AGATCT 1 cut(s) 273
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 266
BmrFI CCNGG 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 286
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 223, 259, 319
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 290, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 223, 259, 319
BseGI GGATG 2 cut(s) 147, 294
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 42
BshFI GGCC 2 cut(s) 128, 268
BsiSI CCGG 3 cut(s) 173, 269, 286
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 128, 268
Bsp143I GATC 5 cut(s) 169, 190, 195, 255, 273
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 3 cut(s) 56, 374, 383
BspANI GGCC 2 cut(s) 128, 268
BspCNI CTCAG 1 cut(s) 43
BspPI GGATC 3 cut(s) 164, 203, 250
BssECI CCNNGG 3 cut(s) 223, 259, 319
BssMI GATC 5 cut(s) 169, 190, 195, 255, 273
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 3 cut(s) 223, 259, 319
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 371
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 1 cut(s) 223
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 147, 294
BstKTI GATC 5 cut(s) 172, 193, 198, 258, 276
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 5 cut(s) 169, 190, 195, 255, 273
BstMWI GCNNNNNNNGC 2 cut(s) 371, 380
BstSCI CCNGG 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 2 cut(s) 128, 268
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 147, 294
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 238
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 3 cut(s) 74, 100, 224
CviJI RGCY 3 cut(s) 128, 268, 325
CviKI_1 RGCY 3 cut(s) 128, 268, 325
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 51
DpnI GATC 5 cut(s) 171, 192, 197, 257, 275
DpnII GATC 5 cut(s) 169, 190, 195, 255, 273
Eco130I CCWWGG 3 cut(s) 223, 259, 319
Eco147I AGGCCT 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoT14I CCWWGG 3 cut(s) 223, 259, 319
ErhI CCWWGG 3 cut(s) 223, 259, 319
FaeI CATG 3 cut(s) 77, 103, 227
FaiI YATR 5 cut(s) 75, 101, 116, 225, 338
FatI CATG 3 cut(s) 73, 99, 223
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 2 cut(s) 154, 301
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 2 cut(s) 104, 150
GluI GCNGC 1 cut(s) 375
HaeIII GGCC 2 cut(s) 128, 268
HapII CCGG 3 cut(s) 173, 269, 286
Hin1II CATG 3 cut(s) 77, 103, 227
HinfI GANTC 3 cut(s) 209, 219, 397
HpaII CCGG 3 cut(s) 173, 269, 286
HphI GGTGA 2 cut(s) 122, 304
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 195, 200
Hpy188III TCNNGA 4 cut(s) 31, 61, 150, 188
Hpy8I GTNNAC 1 cut(s) 238
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 380
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 3 cut(s) 77, 103, 227
Kzo9I GATC 5 cut(s) 169, 190, 195, 255, 273
LpnPI CCDG 8 cut(s) 186, 233, 254, 282, 289, 299, 386, 394
MaeI CTAG 2 cut(s) 104, 150
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 243, 292
MalI GATC 5 cut(s) 171, 192, 197, 257, 275
MboI GATC 5 cut(s) 169, 190, 195, 255, 273
MflI RGATCY 1 cut(s) 273
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 6 cut(s) 46, 71, 118, 130, 139, 257
MslI CAYNNNNRTG 1 cut(s) 151
MspI CCGG 3 cut(s) 173, 269, 286
MspR9I CCNGG 1 cut(s) 286
MwoI GCNNNNNNNGC 2 cut(s) 371, 380
NciI CCSGG 1 cut(s) 286
NcoI CCATGG 1 cut(s) 223
NdeII GATC 5 cut(s) 169, 190, 195, 255, 273
NlaIII CATG 3 cut(s) 77, 103, 227
NmuCI GTSAC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 2 cut(s) 209, 397
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 273
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 151
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 5 cut(s) 169, 190, 195, 255, 273
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 286
SetI ASST 3 cut(s) 98, 207, 243
SmiMI CAYNNNNRTG 1 cut(s) 151
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 3 cut(s) 56, 374, 383
SspMI CTAG 2 cut(s) 104, 150
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 3 cut(s) 223, 259, 319
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 98
TaqI TCGA 4 cut(s) 207, 217, 313, 390
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 209, 397
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 88, 131
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 172
XagI CCTNNNNNAGG 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 149
XspI CTAG 2 cut(s) 104, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.