Rh5DG108700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000576
Physical Location & Seq
Forward (+)
9866409 .. 9867103
695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG108700.1

Sequence Viewer

Length: 618 bp
ATGGCGGTGATGGCAGATCTTGGTCTGCTTATTCTGTTCTTCATTGCGTTGTGGGACGCTGAGGCGGTGTTGAGTTCAGGGGTTGCAGGCGTTGTTGTTTCTGCGCCCAAGCTCGACGGTGACATGAATGAGGCGACCTCCGGGAGGGAGTTGGCATCTAAATGGCAACTGAGCAGCGATCCGGTCTTGGTTTGGTCAAGATCGGATCGGAAAGGTCGATTCCGATCGAGTCCATGGGAAGATGTTCTCTGGCGTGCAAGCGATCTTGATCTCCCGGACGGTGACAGAGCGCGGGTTTGCTCGGACGTAGGGACTATTACTTCTGTTGGTGGTGCTCATCGGCAGATCGACGGTCAGTACGGAGGTAAGAGTGGCTGTCAATTTGCACCTGGGATTCCGGAGCTGAGGGAGGATCTGTTTTTCTCCGACCCGGGTAGATCGTTGGTGGGGATTATGGTGCGTCTCTGCGCATCGGAGAAGCTTACCTTTGATGGTGAGGATGCTTGGTGCAACGACGGGCCCTTCCACGTCTTTGGTGGTCGCGGCTTGCTGGTTGGCGGCGGCGGTGTGCTGGTCCGGCTGGGCCTTCAGTATCAGGTTTTGGGCTGTCTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

21.89

Weight (kDa)

4.81

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 469
AccII CGCG 2 cut(s) 292, 543
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 7 cut(s) 5, 65, 292, 543, 558, 561, 564
AclWI GGATC 3 cut(s) 173, 213, 420
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 144
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 3 cut(s) 112, 403, 481
AluI AGCT 3 cut(s) 112, 403, 481
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 3 cut(s) 173, 213, 420
Ama87I CYCGRG 1 cut(s) 430
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 2 cut(s) 518, 583
ApaI GGGCCC 1 cut(s) 522
ApeKI GCWGC 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 243
AspLEI GCGC 3 cut(s) 106, 292, 470
AspS9I GGNCC 4 cut(s) 518, 519, 574, 583
AsuC2I CCSGG 4 cut(s) 142, 275, 431, 432
AsuHPI GGTGA 4 cut(s) 19, 131, 293, 506
AvaI CYCGRG 1 cut(s) 430
AvaII GGWCC 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 522
BanII GRGCYC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 2 cut(s) 60, 404
BbvI GCAGC 1 cut(s) 186
BccI CCATC 2 cut(s) 4, 485
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 4 cut(s) 142, 275, 431, 432
BcoDI GTCTC 1 cut(s) 467
BglII AGATCT 1 cut(s) 16
BisI GCNGC 4 cut(s) 175, 544, 559, 562
BlsI GCNGC 4 cut(s) 176, 545, 560, 563
Bme1390I CCNGG 5 cut(s) 142, 275, 390, 431, 432
Bme18I GGWCC 1 cut(s) 574
BmeT110I CYCGRG 1 cut(s) 430
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 4 cut(s) 518, 519, 574, 583
BmiI GGNNCC 1 cut(s) 520
BmrFI CCNGG 5 cut(s) 142, 275, 390, 431, 432
BmsI GCATC 3 cut(s) 164, 479, 490
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bpu10I CCTNAGC 2 cut(s) 60, 404
BpuMI CCSGG 4 cut(s) 142, 275, 431, 432
BsaBI GATNNNNATC 2 cut(s) 223, 267
BsaJI CCNNGG 3 cut(s) 233, 389, 430
BsaWI WCCGGW 2 cut(s) 181, 397
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 42
Bse8I GATNNNNATC 2 cut(s) 223, 267
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 233, 389, 430
BseGI GGATG 1 cut(s) 505
BseJI GATNNNNATC 2 cut(s) 223, 267
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 3 cut(s) 51, 161, 395
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 1 cut(s) 186
BseYI CCCAGC 1 cut(s) 580
Bsh1236I CGCG 2 cut(s) 292, 543
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 520, 585
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 6 cut(s) 141, 182, 275, 398, 431, 577
BslFI GGGAC 2 cut(s) 68, 325
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 467
BsmBI CGTCTC 1 cut(s) 467
BsmFI GGGAC 2 cut(s) 68, 325
BsnI GGCC 2 cut(s) 520, 585
BsoBI CYCGRG 1 cut(s) 430
Bsp120I GGGCCC 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 337, 522
Bsp13I TCCGGA 1 cut(s) 397
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 7 cut(s) 5, 65, 292, 543, 558, 561, 564
BspANI GGCC 2 cut(s) 520, 585
BspCNI CTCAG 3 cut(s) 52, 162, 396
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 2 cut(s) 292, 543
BspLI GGNNCC 1 cut(s) 520
BspPI GGATC 3 cut(s) 173, 213, 420
BsrDI GCAATG 1 cut(s) 42
BssECI CCNNGG 3 cut(s) 233, 389, 430
BssT1I CCWWGG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 3 cut(s) 119, 281, 353
BstC8I GCNNGC 4 cut(s) 88, 255, 259, 548
BstDEI CTNAG 3 cut(s) 60, 170, 404
BstDSI CCRYGG 1 cut(s) 233
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 2 cut(s) 292, 543
BstHHI GCGC 3 cut(s) 106, 292, 470
BstMAI GTCTC 1 cut(s) 467
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 2 cut(s) 11, 577
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 5 cut(s) 140, 273, 388, 429, 430
BstSLI GKGCMC 1 cut(s) 522
BstUI CGCG 2 cut(s) 292, 543
BstV1I GCAGC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 16, 412
BstXI CCANNNNNNTGG 1 cut(s) 533
BstYI RGATCY 2 cut(s) 16, 412
BsuRI GGCC 2 cut(s) 520, 585
BtgI CCRYGG 1 cut(s) 233
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 505
Cac8I GCNNGC 4 cut(s) 88, 255, 259, 548
CfoI GCGC 3 cut(s) 106, 292, 470
Cfr13I GGNCC 4 cut(s) 518, 519, 574, 583
Cfr9I CCCGGG 1 cut(s) 430
CseI GACGC 2 cut(s) 65, 449
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 124, 234
CviJI RGCY 9 cut(s) 112, 375, 403, 481, 520, 546, 580, 585, 606
CviKI_1 RGCY 9 cut(s) 112, 375, 403, 481, 520, 546, 580, 585, 606
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 3 cut(s) 60, 170, 404
Eco130I CCWWGG 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 2 cut(s) 127, 237
FaiI YATR 3 cut(s) 125, 235, 455
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 2 cut(s) 68, 325
FatI CATG 2 cut(s) 123, 233
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 4 cut(s) 175, 544, 559, 562
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 4 cut(s) 175, 544, 559, 562
FspI TGCGCA 1 cut(s) 469
GlaI GCGC 3 cut(s) 105, 291, 469
GluI GCNGC 4 cut(s) 175, 544, 559, 562
GsaI CCCAGC 1 cut(s) 584
HaeIII GGCC 2 cut(s) 520, 585
HapII CCGG 6 cut(s) 141, 182, 275, 398, 431, 577
HgaI GACGC 2 cut(s) 65, 449
HhaI GCGC 3 cut(s) 106, 292, 470
Hin1II CATG 2 cut(s) 127, 237
Hin6I GCGC 3 cut(s) 104, 290, 468
HinP1I GCGC 3 cut(s) 104, 290, 468
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 3 cut(s) 219, 229, 394
HpaII CCGG 6 cut(s) 141, 182, 275, 398, 431, 577
HphI GGTGA 4 cut(s) 19, 131, 293, 506
Hpy188I TCNGA 6 cut(s) 205, 210, 224, 304, 427, 475
Hpy188III TCNNGA 3 cut(s) 198, 266, 398
Hpy99I CGWCG 3 cut(s) 119, 353, 518
HpyAV CCTTC 2 cut(s) 532, 596
HpyCH4III ACNGT 3 cut(s) 119, 281, 353
HpyCH4IV ACGT 2 cut(s) 306, 528
HpyCH4V TGCA 4 cut(s) 86, 257, 386, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 577
HpyF3I CTNAG 3 cut(s) 60, 170, 404
HpySE526I ACGT 2 cut(s) 306, 528
Hsp92II CATG 2 cut(s) 127, 237
HspAI GCGC 3 cut(s) 104, 290, 468
Kpn2I TCCGGA 1 cut(s) 397
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 3 cut(s) 164, 479, 490
MaeII ACGT 2 cut(s) 306, 528
MaeIII GTNAC 2 cut(s) 119, 281
MboII GAAGA 2 cut(s) 31, 251
MflI RGATCY 2 cut(s) 16, 412
MhlI GDGCHC 2 cut(s) 337, 522
MluCI AATT 1 cut(s) 380
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 8 cut(s) 55, 124, 138, 148, 356, 399, 403, 490
MroI TCCGGA 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 243
MseI TTAA 1 cut(s) 616
MslI CAYNNNNRTG 1 cut(s) 160
MspI CCGG 6 cut(s) 141, 182, 275, 398, 431, 577
MspR9I CCNGG 5 cut(s) 142, 275, 390, 431, 432
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 2 cut(s) 292, 543
MwoI GCNNNNNNNGC 2 cut(s) 11, 577
NciI CCSGG 4 cut(s) 142, 275, 431, 432
NcoI CCATGG 1 cut(s) 233
NlaIII CATG 2 cut(s) 127, 237
NlaIV GGNNCC 1 cut(s) 520
NmuCI GTSAC 2 cut(s) 119, 281
NsbI TGCGCA 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 2 cut(s) 219, 394
PfoI TCCNGGA 2 cut(s) 140, 273
PkrI GCNGC 4 cut(s) 176, 545, 560, 563
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 520
PspOMI GGGCCC 1 cut(s) 518
PspPI GGNCC 4 cut(s) 518, 519, 574, 583
PsuI RGATCY 2 cut(s) 16, 412
PvuI CGATCG 1 cut(s) 227
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 160
SaqAI TTAA 1 cut(s) 616
SatI GCNGC 4 cut(s) 175, 544, 559, 562
Sau96I GGNCC 4 cut(s) 518, 519, 574, 583
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 5 cut(s) 142, 275, 390, 431, 432
SduI GDGCHC 2 cut(s) 337, 522
SfaNI GCATC 3 cut(s) 164, 479, 490
SinI GGWCC 1 cut(s) 574
SmaI CCCGGG 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 160
Sse9I AATT 1 cut(s) 380
SsiI CCGC 7 cut(s) 5, 65, 292, 543, 558, 561, 564
StyD4I CCNGG 5 cut(s) 140, 273, 388, 429, 430
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 3 cut(s) 119, 281, 353
TaiI ACGT 2 cut(s) 309, 531
TaqI TCGA 4 cut(s) 114, 217, 227, 348
TasI AATT 1 cut(s) 380
TauI GCSGC 3 cut(s) 546, 561, 564
TfiI GAWTC 2 cut(s) 219, 394
Tru1I TTAA 1 cut(s) 616
Tru9I TTAA 1 cut(s) 616
TseFI GTSAC 2 cut(s) 119, 281
TseI GCWGC 1 cut(s) 174
Tsp45I GTSAC 2 cut(s) 119, 281
TspDTI ATGAA 2 cut(s) 31, 140
TspGWI ACGGA 1 cut(s) 375
TspMI CCCGGG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 574
XagI CCTNNNNNAGG 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XmaI CCCGGG 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.