Rh5DG298500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000576
Physical Location & Seq
Forward (+)
37552334 .. 37556331
3998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG298500.1

Sequence Viewer

Length: 594 bp
ATGGCGGATCTTGGTCTGCTTATTCTCTTCCTCGTGGCACTGGGTAGCGCTGAGGCTGTGTTGGTGTCGGGGGTTGGAGGCGTTGTTGGATCCGCGTCCAAGCTTGATGGTGAAGTGCAAGATGCGGCCTCGTTTGCGGGGATGGCATCTCGGTGGTGGGTGAGTGGTGATCCGGTCTTGGTTTGGTCAAGATCGGATCGGCGGGGTCGATTCCGATCAGATCCAAGGGACAATGGTGCATCTAAGAATGGCATCTTGATTAACCAAATCTGGTTTGGTGTTCCTATTGATAGTGATGATGGATCTACTCTTGATCCATCAATCTATGGCGAAGGCCAGGTTACTCTTGTTGGGGGTGGTGGTGATTTGTGGTGGGATTGCGTGCAACAGTGTGGCGAATCTGGGAGAGGTGATAGCTTCGGCTTCTTCTCCTCTTCCTTGGAATCTTCTGATGGTTGTAGCGCACAGCAGAATGCACAGAGACAACAGAACACCTGGAATCAGCAATCGGGGGGCCCGGAAAGCCACTTCTCCCCGTCTCGACTATATGTGAATATATCCATTTCCCATAAAAGAGTGTCATACTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

20.99

Weight (kDa)

4.68

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 5 cut(s) 5, 93, 125, 137, 202
AclWI GGATC 8 cut(s) 15, 84, 97, 164, 204, 215, 308, 310
AfeI AGCGCT 1 cut(s) 49
AjnI CCWGG 2 cut(s) 336, 494
AluBI AGCT 2 cut(s) 103, 417
AluI AGCT 2 cut(s) 103, 417
Alw26I GTCTC 2 cut(s) 475, 543
AlwI GGATC 8 cut(s) 15, 84, 97, 164, 204, 215, 308, 310
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 3 cut(s) 126, 334, 514
ApaI GGGCCC 1 cut(s) 518
AspLEI GCGC 2 cut(s) 50, 464
AspS9I GGNCC 2 cut(s) 514, 515
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 5 cut(s) 122, 172, 179, 374, 422
BaeGI GKGCMC 1 cut(s) 518
BamHI GGATCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 518
BauI CACGAG 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 51
BccI CCATC 5 cut(s) 101, 136, 293, 325, 446
BciT130I CCWGG 2 cut(s) 338, 496
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 2 cut(s) 475, 543
BfoI RGCGCY 1 cut(s) 51
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 3 cut(s) 338, 496, 518
BmgT120I GGNCC 2 cut(s) 514, 515
BmiI GGNNCC 3 cut(s) 91, 515, 516
BmrFI CCNGG 3 cut(s) 338, 496, 518
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 4 cut(s) 112, 155, 248, 261
BmuI ACTGGG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 224, 438
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bse1I ACTGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 214
BseBI CCWGG 2 cut(s) 338, 496
BseDI CCNNGG 2 cut(s) 224, 438
BseGI GGATG 1 cut(s) 147
BseJI GATNNNNATC 1 cut(s) 214
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 128, 336, 516
BsiSI CCGG 2 cut(s) 173, 518
BslFI GGGAC 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 475, 543
BsmBI CGTCTC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 3 cut(s) 128, 336, 516
Bsp120I GGGCCC 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
BspACI CCGC 5 cut(s) 5, 93, 125, 137, 202
BspANI GGCC 3 cut(s) 128, 336, 516
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 3 cut(s) 91, 515, 516
BspPI GGATC 8 cut(s) 15, 84, 97, 164, 204, 215, 308, 310
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 224, 438
BssMI GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 2 cut(s) 224, 438
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 338, 496
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 2 cut(s) 32, 439
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 2 cut(s) 51, 243
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 464
BstKTI GATC 9 cut(s) 10, 92, 172, 194, 199, 218, 223, 305, 316
BstMAI GTCTC 2 cut(s) 475, 543
BstMBI GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
BstMWI GCNNNNNNNGC 3 cut(s) 134, 143, 522
BstNI CCWGG 2 cut(s) 338, 496
BstSCI CCNGG 3 cut(s) 336, 494, 516
BstSLI GKGCMC 1 cut(s) 518
BstUI CGCG 1 cut(s) 95
BstX2I RGATCY 4 cut(s) 7, 89, 220, 302
BstYI RGATCY 4 cut(s) 7, 89, 220, 302
BsuRI GGCC 3 cut(s) 128, 336, 516
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 38, 395
Cac8I GCNNGC 1 cut(s) 383
CfoI GCGC 2 cut(s) 50, 464
Cfr13I GGNCC 2 cut(s) 514, 515
CseI GACGC 1 cut(s) 84
CviJI RGCY 8 cut(s) 56, 103, 128, 336, 417, 423, 516, 525
CviKI_1 RGCY 8 cut(s) 56, 103, 128, 336, 417, 423, 516, 525
DdeI CTNAG 2 cut(s) 51, 243
DpnI GATC 9 cut(s) 9, 91, 171, 193, 198, 217, 222, 304, 315
DpnII GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
Eam1104I CTCTTC 2 cut(s) 32, 439
EarI CTCTTC 2 cut(s) 32, 439
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 224, 438
Eco24I GRGCYC 1 cut(s) 518
Eco47III AGCGCT 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 336, 494
EcoT14I CCWWGG 2 cut(s) 224, 438
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 2 cut(s) 224, 438
Esp3I CGTCTC 1 cut(s) 543
FaiI YATR 6 cut(s) 327, 547, 549, 557, 570, 583
FaqI GGGAC 1 cut(s) 242
FauI CCCGC 2 cut(s) 130, 195
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 154
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 1 cut(s) 126
GlaI GCGC 2 cut(s) 49, 463
GluI GCNGC 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 3 cut(s) 128, 336, 516
HapII CCGG 2 cut(s) 173, 518
HgaI GACGC 1 cut(s) 84
HhaI GCGC 2 cut(s) 50, 464
Hin6I GCGC 2 cut(s) 48, 462
HinP1I GCGC 2 cut(s) 48, 462
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 4 cut(s) 210, 398, 443, 499
HpaII CCGG 2 cut(s) 173, 518
HphI GGTGA 5 cut(s) 122, 172, 179, 374, 422
Hpy188I TCNGA 4 cut(s) 196, 215, 220, 451
Hpy188III TCNNGA 4 cut(s) 189, 256, 311, 540
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 118, 239, 385, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 143, 522
HpyF3I CTNAG 2 cut(s) 51, 243
HspAI GCGC 2 cut(s) 48, 462
Kzo9I GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
LpnPI CCDG 9 cut(s) 26, 186, 256, 323, 350, 387, 481, 508, 531
LweI GCATC 4 cut(s) 112, 155, 248, 261
MaeIII GTNAC 1 cut(s) 340
MalI GATC 9 cut(s) 9, 91, 171, 193, 198, 217, 222, 304, 315
MboI GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
MboII GAAGA 4 cut(s) 19, 418, 426, 438
MflI RGATCY 4 cut(s) 7, 89, 220, 302
MhlI GDGCHC 1 cut(s) 518
MmeI TCCRAC 2 cut(s) 55, 67
MnlI CCTC 6 cut(s) 41, 46, 71, 139, 401, 442
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 151
MspI CCGG 2 cut(s) 173, 518
MspR9I CCNGG 3 cut(s) 338, 496, 518
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 2 cut(s) 338, 496
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 3 cut(s) 134, 143, 522
NciI CCSGG 1 cut(s) 518
NdeII GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
NlaIV GGNNCC 3 cut(s) 91, 515, 516
PcsI WCGNNNNNNNCGW 1 cut(s) 205
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 4 cut(s) 210, 398, 443, 499
PkrI GCNGC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 336, 494
PspGI CCWGG 2 cut(s) 336, 494
PspN4I GGNNCC 3 cut(s) 91, 515, 516
PspOMI GGGCCC 1 cut(s) 514
PspPI GGNCC 2 cut(s) 514, 515
PsuI RGATCY 4 cut(s) 7, 89, 220, 302
RseI CAYNNNNRTG 1 cut(s) 151
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 9 cut(s) 7, 89, 169, 191, 196, 215, 220, 302, 313
Sau96I GGNCC 2 cut(s) 514, 515
ScrFI CCNGG 3 cut(s) 338, 496, 518
SduI GDGCHC 1 cut(s) 518
SetI ASST 5 cut(s) 105, 342, 412, 419, 497
SfaNI GCATC 4 cut(s) 112, 155, 248, 261
SmiMI CAYNNNNRTG 1 cut(s) 151
SsiI CCGC 5 cut(s) 5, 93, 125, 137, 202
StyD4I CCNGG 3 cut(s) 336, 494, 516
StyI CCWWGG 2 cut(s) 224, 438
TaaI ACNGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 208, 541
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 4 cut(s) 210, 398, 443, 499
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 45, 395
TspRI CASTG 2 cut(s) 45, 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.