Rh6DG231300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000576
Physical Location & Seq
Forward (+)
41258557 .. 41259117
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG231300.1

Sequence Viewer

Length: 561 bp
ATGCTAAGGTTTAAGAATCCTCTCCCTCAGGGCGGCGGCTTCTCGTATTGTCACTGTTGCTTGTCAATGGTGGTTATGGCAGATCTGGGTCTGCTTATTATTTTCTTCATTGAGCTAGGTGGGGCTGAGGCTATGTTGATTTCGTGGGACGGGGATGCGGTTGTTTCTGAGTCCAAGCCCGATGGTAACGTGAAAGATGTGGCCTTGTTGGCGGGGATGGCATCTAGGTGGCAGATGAGCGGCGATCCGATCTTGGGTTGGTTTAGATCTGTTCGGAAGGTTCGGTTCCGATCACATGCTGATTTTTGGAATGCTGGTGATCTTGCTGTCGTGGAGGGTGATGATGTGATGGTTTGCTCAACCTCGAGGACTGCTACTGCTGATGGGGGTCCAAGGTTGCCGGAGGTGAGGTCGGCTCTGCTTCCCTCGGATTTGGACCTTTTGTTGGCGAGGAGGATAACTTCGCTCTGCTTGTCGGAGGAATCTGCCTTTGATGGTGGTAACCCGGTGGCCGGGATGATGGTTGCGAGGTTTAAAATTGGTATATGTACAGATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.02

Weight (kDa)

4.98

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 240
AciI CCGC 5 cut(s) 33, 36, 158, 212, 240
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 510
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 4 cut(s) 32, 254, 445, 512
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 364
AoxI GGCC 2 cut(s) 201, 510
AspS9I GGNCC 2 cut(s) 389, 436
AsuC2I CCSGG 2 cut(s) 506, 514
AsuHPI GGTGA 3 cut(s) 329, 350, 418
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 2 cut(s) 389, 436
AxyI CCTNAGG 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 126
BccI CCATC 6 cut(s) 176, 211, 343, 377, 488, 514
BcnI CCSGG 2 cut(s) 506, 514
BfaI CTAG 2 cut(s) 116, 225
BglI GCCNNNNNGGC 1 cut(s) 209
BglII AGATCT 2 cut(s) 82, 266
BisI GCNGC 3 cut(s) 34, 37, 241
BlsI GCNGC 3 cut(s) 35, 38, 242
Bme1390I CCNGG 2 cut(s) 506, 514
Bme18I GGWCC 2 cut(s) 389, 436
BmeT110I CYCGRG 1 cut(s) 364
BmgT120I GGNCC 2 cut(s) 389, 436
BmiI GGNNCC 2 cut(s) 287, 390
BmrFI CCNGG 2 cut(s) 506, 514
BmsI GCATC 2 cut(s) 145, 230
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu10I CCTNAGC 2 cut(s) 5, 126
BpuMI CCSGG 2 cut(s) 506, 514
BsaJI CCNNGG 2 cut(s) 392, 426
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 254, 445, 512
Bse21I CCTNAGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 392, 426
BseGI GGATG 3 cut(s) 160, 222, 522
BseLI CCNNNNNNNGG 4 cut(s) 32, 254, 445, 512
BseMII CTCAG 3 cut(s) 41, 117, 159
BseRI GAGGAG 1 cut(s) 466
BshFI GGCC 2 cut(s) 203, 512
BsiHKCI CYCGRG 1 cut(s) 364
BsiSI CCGG 3 cut(s) 401, 506, 513
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 4 cut(s) 32, 254, 445, 512
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 2 cut(s) 203, 512
BsoBI CYCGRG 1 cut(s) 364
Bsp1407I TGTACA 1 cut(s) 548
Bsp143I GATC 6 cut(s) 82, 244, 249, 266, 290, 319
BspACI CCGC 5 cut(s) 33, 36, 158, 212, 240
BspANI GGCC 2 cut(s) 203, 512
BspCNI CTCAG 3 cut(s) 40, 118, 160
BspLI GGNNCC 2 cut(s) 287, 390
BspPI GGATC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 548
BssECI CCNNGG 2 cut(s) 392, 426
BssMI GATC 6 cut(s) 82, 244, 249, 266, 290, 319
BssT1I CCWWGG 1 cut(s) 392
Bst4CI ACNGT 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 548
BstDEI CTNAG 4 cut(s) 5, 27, 126, 168
BstEII GGTNACC 1 cut(s) 500
BstF5I GGATG 3 cut(s) 160, 222, 522
BstKTI GATC 6 cut(s) 85, 247, 252, 269, 293, 322
BstMBI GATC 6 cut(s) 82, 244, 249, 266, 290, 319
BstMWI GCNNNNNNNGC 2 cut(s) 209, 218
BstNSI RCATGY 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 2 cut(s) 504, 512
BstX2I RGATCY 2 cut(s) 82, 266
BstYI RGATCY 2 cut(s) 82, 266
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 2 cut(s) 203, 512
BtsCI GGATG 3 cut(s) 160, 222, 522
BtsIMutI CAGTG 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 389, 436
Csp6I GTAC 1 cut(s) 549
CviAII CATG 1 cut(s) 296
CviJI RGCY 8 cut(s) 39, 115, 125, 131, 178, 203, 416, 512
CviKI_1 RGCY 8 cut(s) 39, 115, 125, 131, 178, 203, 416, 512
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 4 cut(s) 5, 27, 126, 168
DpnI GATC 6 cut(s) 84, 246, 251, 268, 292, 321
DpnII GATC 6 cut(s) 82, 244, 249, 266, 290, 319
DraI TTTAAA 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 389, 436
Eco81I CCTNAGG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 364
Eco91I GGTNACC 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 1 cut(s) 299
FaiI YATR 5 cut(s) 77, 134, 297, 545, 547
FaqI GGGAC 1 cut(s) 161
FatI CATG 1 cut(s) 295
FauI CCCGC 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 34, 37, 241
FokI GGATG 3 cut(s) 167, 229, 529
Fsp4HI GCNGC 3 cut(s) 34, 37, 241
FspBI CTAG 2 cut(s) 116, 225
GluI GCNGC 3 cut(s) 34, 37, 241
HaeIII GGCC 2 cut(s) 203, 512
HapII CCGG 3 cut(s) 401, 506, 513
Hin1II CATG 1 cut(s) 299
HinfI GANTC 3 cut(s) 16, 170, 482
HpaII CCGG 3 cut(s) 401, 506, 513
HphI GGTGA 3 cut(s) 329, 350, 418
Hpy188I TCNGA 6 cut(s) 169, 249, 276, 290, 430, 478
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 189
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 218
HpyF3I CTNAG 4 cut(s) 5, 27, 126, 168
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 1 cut(s) 299
Kzo9I GATC 6 cut(s) 82, 244, 249, 266, 290, 319
LpnPI CCDG 6 cut(s) 14, 71, 300, 414, 519, 526
LweI GCATC 2 cut(s) 145, 230
MaeI CTAG 2 cut(s) 116, 225
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 50, 185, 500
MalI GATC 6 cut(s) 84, 246, 251, 268, 292, 321
MbiI CCGCTC 1 cut(s) 240
MboI GATC 6 cut(s) 82, 244, 249, 266, 290, 319
MboII GAAGA 1 cut(s) 97
MflI RGATCY 2 cut(s) 82, 266
MluCI AATT 1 cut(s) 537
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 456
MseI TTAA 2 cut(s) 12, 534
MslI CAYNNNNRTG 1 cut(s) 226
MspI CCGG 3 cut(s) 401, 506, 513
MspR9I CCNGG 2 cut(s) 506, 514
Mva1269I GAATGC 1 cut(s) 316
MwoI GCNNNNNNNGC 2 cut(s) 209, 218
NciI CCSGG 2 cut(s) 506, 514
NdeII GATC 6 cut(s) 82, 244, 249, 266, 290, 319
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 2 cut(s) 287, 390
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 1 cut(s) 299
PaeR7I CTCGAG 1 cut(s) 364
PctI GAATGC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 16, 482
PkrI GCNGC 3 cut(s) 35, 38, 242
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspEI GGTNACC 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 287, 390
PspPI GGNCC 2 cut(s) 389, 436
PspXI VCTCGAGB 1 cut(s) 364
PsuI RGATCY 2 cut(s) 82, 266
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 226
SaqAI TTAA 2 cut(s) 12, 534
SatI GCNGC 3 cut(s) 34, 37, 241
Sau3AI GATC 6 cut(s) 82, 244, 249, 266, 290, 319
Sau96I GGNCC 2 cut(s) 389, 436
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 506, 514
SfaNI GCATC 2 cut(s) 145, 230
Sfr274I CTCGAG 1 cut(s) 364
SinI GGWCC 2 cut(s) 389, 436
SlaI CTCGAG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
Sse9I AATT 1 cut(s) 537
SsiI CCGC 5 cut(s) 33, 36, 158, 212, 240
SspMI CTAG 2 cut(s) 116, 225
StyD4I CCNGG 2 cut(s) 504, 512
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 192
TaqI TCGA 1 cut(s) 365
TasI AATT 1 cut(s) 537
TatI WGTACW 1 cut(s) 548
TauI GCSGC 3 cut(s) 36, 39, 243
TfiI GAWTC 2 cut(s) 16, 482
Tru1I TTAA 2 cut(s) 12, 534
Tru9I TTAA 2 cut(s) 12, 534
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 97
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 389, 436
XceI RCATGY 1 cut(s) 299
XhoI CTCGAG 1 cut(s) 364
XspI CTAG 2 cut(s) 116, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.