Rroxscaffold_5G00344480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000576
Physical Location & Seq
Forward (+)
14592632 .. 14593257
626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344480.1

Sequence Viewer

Length: 531 bp
ATGGCAGATCTTGGTCTGCTTATTCTCTTCCTCGCTGCGCTGTGTAGCGCCGAGGCAGTGTTGATGTCGGGGGTTGGGGGTGATGTTGGATCTGCGTCCAAACTCGATGGTGAAGTGCAGGATGCGGCCTCGATGCAGGGATGGCTTCTAGGTGCTGATATTTGGCGCGCCGTTATCGTGGAGGGTGTTGACGGCGGTGCTCTTGGGTATTCTGGCGGCAAGTGTGGAGGCAAGGGCGGCTATCAACTTGTATCGGTTATCGAAGGTAAGGAGGACCCTGCTTTCCTCGGGCCTGGACCCTTCGTTAGCGGGGATGACAGCTTGGTTCTCCTCGATGGTGGCTGGAACAGTGACGGGATCTCTGAAATCTGTGGCGGCAGCGGCGGTTTGCAGATCGGAGATGATGGTGGGGTCTGTGGGGTGTCCTTAGAAATCTCTACTTGGGCCCTCTGGGCTTCATTCTGGTTTGTGAGTTTTACATGCTGGGCTTGGGTGGGCTGGGCCCTCAGTTTTAAGGGGCTGACTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

17.87

Weight (kDa)

4.05

Isoelectric Point (pI)

24.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 168
AciI CCGC 8 cut(s) 125, 195, 216, 237, 309, 375, 381, 384
AclWI GGATC 2 cut(s) 97, 365
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 1 cut(s) 321
AluI AGCT 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 202
AlwI GGATC 2 cut(s) 97, 365
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 4 cut(s) 126, 290, 444, 501
ApaI GGGCCC 2 cut(s) 448, 505
ApeKI GCWGC 2 cut(s) 35, 378
AscI GGCGCGCC 1 cut(s) 166
AspLEI GCGC 4 cut(s) 40, 50, 168, 170
AspS9I GGNCC 7 cut(s) 274, 290, 296, 444, 445, 501, 502
AsuHPI GGTGA 2 cut(s) 92, 122
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 2 cut(s) 274, 296
BaeGI GKGCMC 2 cut(s) 448, 505
BanII GRGCYC 2 cut(s) 448, 505
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 2 cut(s) 22, 390
BccI CCATC 4 cut(s) 101, 135, 329, 398
BceAI ACGGC 2 cut(s) 155, 208
BciT130I CCWGG 1 cut(s) 294
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 51
BglI GCCNNNNNGGC 1 cut(s) 452
BglII AGATCT 1 cut(s) 7
BisI GCNGC 7 cut(s) 36, 126, 217, 238, 376, 379, 382
BlsI GCNGC 7 cut(s) 37, 127, 218, 239, 377, 380, 383
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 2 cut(s) 274, 296
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 7 cut(s) 274, 290, 296, 444, 445, 501, 502
BmiI GGNNCC 4 cut(s) 276, 298, 446, 503
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 2 cut(s) 112, 123
BsaJI CCNNGG 2 cut(s) 51, 286
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 51, 286
BseGI GGATG 3 cut(s) 127, 146, 319
BseMII CTCAG 1 cut(s) 520
BsePI GCGCGC 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 2 cut(s) 448, 505
BseXI GCAGC 2 cut(s) 22, 390
BseYI CCCAGC 2 cut(s) 483, 498
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 4 cut(s) 128, 292, 446, 503
BsiHKAI GWGCWC 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 287
BsnI GGCC 4 cut(s) 128, 292, 446, 503
BsoBI CYCGRG 1 cut(s) 287
Bsp120I GGGCCC 2 cut(s) 444, 501
Bsp1286I GDGCHC 3 cut(s) 202, 448, 505
Bsp143I GATC 4 cut(s) 7, 89, 357, 393
BspACI CCGC 8 cut(s) 125, 195, 216, 237, 309, 375, 381, 384
BspANI GGCC 4 cut(s) 128, 292, 446, 503
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 4 cut(s) 276, 298, 446, 503
BspPI GGATC 2 cut(s) 97, 365
BssECI CCNNGG 2 cut(s) 51, 286
BssHII GCGCGC 1 cut(s) 166
BssMI GATC 4 cut(s) 7, 89, 357, 393
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 2 cut(s) 427, 506
BstF5I GGATG 3 cut(s) 127, 146, 319
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 4 cut(s) 40, 50, 168, 170
BstKTI GATC 4 cut(s) 10, 92, 360, 396
BstMBI GATC 4 cut(s) 7, 89, 357, 393
BstMWI GCNNNNNNNGC 4 cut(s) 142, 237, 381, 452
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 292
BstSLI GKGCMC 2 cut(s) 448, 505
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 22, 390
BstX2I RGATCY 3 cut(s) 7, 89, 357
BstYI RGATCY 3 cut(s) 7, 89, 357
BsuRI GGCC 4 cut(s) 128, 292, 446, 503
BtsCI GGATG 3 cut(s) 127, 146, 319
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 355
Cac8I GCNNGC 1 cut(s) 168
CfoI GCGC 4 cut(s) 40, 50, 168, 170
Cfr13I GGNCC 7 cut(s) 274, 290, 296, 444, 445, 501, 502
CseI GACGC 1 cut(s) 84
CviAII CATG 1 cut(s) 480
DdeI CTNAG 2 cut(s) 427, 506
DpnI GATC 4 cut(s) 9, 91, 359, 395
DpnII GATC 4 cut(s) 7, 89, 357, 393
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 448, 505
Eco47I GGWCC 2 cut(s) 274, 296
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 3 cut(s) 274, 445, 502
EcoRII CCWGG 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 448, 505
FaeI CATG 1 cut(s) 483
FaiI YATR 1 cut(s) 481
FatI CATG 1 cut(s) 479
FauI CCCGC 1 cut(s) 302
Fnu4HI GCNGC 7 cut(s) 36, 126, 217, 238, 376, 379, 382
FokI GGATG 3 cut(s) 134, 153, 326
FriOI GRGCYC 2 cut(s) 448, 505
Fsp4HI GCNGC 7 cut(s) 36, 126, 217, 238, 376, 379, 382
FspBI CTAG 1 cut(s) 149
GlaI GCGC 4 cut(s) 39, 49, 167, 169
GluI GCNGC 7 cut(s) 36, 126, 217, 238, 376, 379, 382
GsaI CCCAGC 2 cut(s) 487, 502
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 4 cut(s) 128, 292, 446, 503
HgaI GACGC 1 cut(s) 84
HhaI GCGC 4 cut(s) 40, 50, 168, 170
Hin1II CATG 1 cut(s) 483
Hin6I GCGC 4 cut(s) 38, 48, 166, 168
HinP1I GCGC 4 cut(s) 38, 48, 166, 168
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 1 cut(s) 523
HphI GGTGA 2 cut(s) 92, 122
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 2 cut(s) 364, 398
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 2 cut(s) 257, 310
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 3 cut(s) 118, 136, 391
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 237, 381, 452
HpyF3I CTNAG 2 cut(s) 427, 506
Hsp92II CATG 1 cut(s) 483
HspAI GCGC 4 cut(s) 38, 48, 166, 168
Kzo9I GATC 4 cut(s) 7, 89, 357, 393
Lsp1109I GCAGC 2 cut(s) 22, 390
LweI GCATC 2 cut(s) 112, 123
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 350
MalI GATC 4 cut(s) 9, 91, 359, 395
MboI GATC 4 cut(s) 7, 89, 357, 393
MboII GAAGA 1 cut(s) 19
MflI RGATCY 3 cut(s) 7, 89, 357
MhlI GDGCHC 3 cut(s) 202, 448, 505
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 67
MseI TTAA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 381
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 142, 237, 381, 452
NdeII GATC 4 cut(s) 7, 89, 357, 393
NlaIII CATG 1 cut(s) 483
NlaIV GGNNCC 4 cut(s) 276, 298, 446, 503
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 483
PalAI GGCGCGCC 1 cut(s) 166
PauI GCGCGC 1 cut(s) 166
PkrI GCNGC 7 cut(s) 37, 127, 218, 239, 377, 380, 383
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 2 cut(s) 483, 498
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 4 cut(s) 276, 298, 446, 503
PspOMI GGGCCC 2 cut(s) 444, 501
PspPI GGNCC 7 cut(s) 274, 290, 296, 444, 445, 501, 502
PspPPI RGGWCCY 1 cut(s) 274
PsuI RGATCY 3 cut(s) 7, 89, 357
PteI GCGCGC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 7 cut(s) 36, 126, 217, 238, 376, 379, 382
Sau3AI GATC 4 cut(s) 7, 89, 357, 393
Sau96I GGNCC 7 cut(s) 274, 290, 296, 444, 445, 501, 502
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 3 cut(s) 202, 448, 505
SetI ASST 3 cut(s) 154, 268, 323
SfaNI GCATC 2 cut(s) 112, 123
SgsI GGCGCGCC 1 cut(s) 166
SinI GGWCC 2 cut(s) 274, 296
SsiI CCGC 8 cut(s) 125, 195, 216, 237, 309, 375, 381, 384
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 292
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 4 cut(s) 105, 131, 261, 333
TauI GCSGC 5 cut(s) 128, 219, 240, 378, 384
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 2 cut(s) 63, 355
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 2 cut(s) 35, 378
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 2 cut(s) 63, 355
VpaK11BI GGWCC 2 cut(s) 274, 296
XceI RCATGY 1 cut(s) 483
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.