AT4G01720
WRKY Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002724
Physical Location & Seq
Forward (+)
744804 .. 748554
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01720.1

Sequence Viewer

Length: 1470 bp
ATGGAAGAACATATTCAAGATCGCCGTGAAATTGCGTTCTTACACTCAGGAGAATTTCTCCACGGAGATTCTGACTCAAAGGATCATCAACCGAACGAGTCTCCGGTGGAACGTCATCACGAGTCGTCTATCAAAGAAGTTGATTTCTTCGCTGCTAAAAGTCAGCCGTTTGATCTTGGTCATGTGAGAACAACGACGATCGTTGGATCATCTGGTTTTAATGATGGATTAGGTTTGGTAAATTCATGTCATGGAACATCAAGCAATGATGGCGATGACAAAACCAAAACTCAAATTAGTAGACTGAAGTTGGAGCTAGAGAGGCTTCACGAGGAGAATCACAAACTGAAGCATTTATTAGATGAGGTCAGTGAGAGTTACAACGACCTCCAAAGAAGAGTTTTGTTAGCAAGACAAACACAAGTGGAAGGTCTTCATCATAAACAACATGAGGATGTACCTCAAGCTGGTTCCTCACAAGCTCTAGAGAACAGAAGACCAAAGGATATGAACCATGAAACTCCGGCCACCACCTTGAAACGACGGTCTCCAGACGACGTGGATGGTCGTGATATGCACCGAGGATCACCAAAAACTCCTCGAATAGACCAAAACAAGAGTACTAATCATGAAGAACAACAAAACCCTCATGATCAATTACCCTATAGAAAAGCTAGGGTTTCCGTTAGAGCTAGATCTGATGCCACTACGGTAAATGACGGATGTCAATGGAGAAAATACGGTCAGAAAATGGCGAAAGGGAATCCATGTCCTCGCGCTTATTATCGTTGCACCATGGCCGTTGGATGTCCTGTCCGTAAACAGGTCCAACGATGCGCGGAGGATACAACTATCTTGACAACAACGTACGAAGGAAACCATAACCATCCTCTTCCCCCGTCAGCCACAGCCATGGCTGCAACCACCTCCGCCGCAGCAGCCATGCTCTTATCAGGCTCCTCCTCCAGCAACCTCCACCAAACACTCTCTAGCCCCTCCGCCACGTCATCATCATCCTTCTACCATAACTTCCCATACACCTCCACAATCGCAACACTCTCTGCCTCAGCTCCTTTCCCCACCATAACCTTAGACCTCACCAACCCACCTCGACCGCTACAACCGCCACCGCAGTTTCTAAGCCAGTATGGTCCCGCCGCGTTTTTACCAAACGCTAATCAAATTAGGTCTATGAATAATAATAACCAGCAGTTATTAATACCTAATTTGTTTGGCCCACAAGCCCCACCACGTGAAATGGTCGATTCAGTTAGGGCTGCGATTGCGATGGATCCGAACTTCACGGCGGCACTTGCGGCCGCGATCTCAAACATTATCGGAGGAGGTAATAACGACAACAATAATAATACTGATATTAATGATAACAAGGTTGATGCAAAAAGTGGAGGGAGTAGTAACGGAGATTCGCCACAGCTTCCTCAGTCTTGCACCACTTTCTCTACAAACTAA

Protein Analysis

489

Amino Acids

53.71

Weight (kDa)

6.51

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 239 - 297 1.4e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AccII CGCG 4 cut(s) 777, 839, 1160, 1322
AclWI GGATC 5 cut(s) 90, 214, 592, 1286, 1299
AcoI YGGCCR 3 cut(s) 525, 798, 1317
AcsI RAATTY 2 cut(s) 53, 241
AcuI CTGAAG 2 cut(s) 326, 368
AcvI CACGTG 1 cut(s) 1253
AdeI CACNNNGTG 1 cut(s) 1253
AfaI GTAC 3 cut(s) 459, 622, 869
AfiI CCNNNNNNNGG 2 cut(s) 467, 823
AgsI TTSAA 2 cut(s) 17, 538
AjiI CACGTC 2 cut(s) 559, 1005
AluBI AGCT 7 cut(s) 316, 467, 482, 674, 692, 1070, 1435
AluI AGCT 7 cut(s) 316, 467, 482, 674, 692, 1070, 1435
Alw26I GTCTC 2 cut(s) 105, 552
AlwI GGATC 5 cut(s) 90, 214, 592, 1286, 1299
AoxI GGCC 4 cut(s) 525, 798, 1234, 1317
ApeKI GCWGC 5 cut(s) 152, 917, 935, 938, 1277
ApoI RAATTY 2 cut(s) 53, 241
AseI ATTAAT 2 cut(s) 1217, 1377
Asp700I GAANNNNTTC 2 cut(s) 12, 432
AspLEI GCGC 2 cut(s) 779, 839
AspS9I GGNCC 3 cut(s) 826, 1151, 1235
AsuHPI GGTGA 2 cut(s) 579, 1090
AvaII GGWCC 2 cut(s) 826, 1151
BamHI GGATCC 1 cut(s) 1291
BauI CACGAG 2 cut(s) 119, 329
BbrPI CACGTG 1 cut(s) 1253
BbsI GAAGAC 2 cut(s) 425, 502
BbvCI CCTCAGC 1 cut(s) 1066
BbvI GCAGC 5 cut(s) 139, 904, 947, 950, 1264
BccI CCATC 5 cut(s) 218, 263, 557, 894, 1282
BceAI ACGGC 4 cut(s) 9, 151, 785, 1320
BciVI GTATCC 1 cut(s) 838
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 2 cut(s) 105, 552
BfaI CTAG 5 cut(s) 317, 485, 675, 693, 990
BfmI CTRYAG 1 cut(s) 664
BfuI GTATCC 1 cut(s) 838
BglII AGATCT 1 cut(s) 695
BmcAI AGTACT 1 cut(s) 622
Bme18I GGWCC 2 cut(s) 826, 1151
BmgBI CACGTC 2 cut(s) 559, 1005
BmgT120I GGNCC 3 cut(s) 826, 1151, 1235
BmiI GGNNCC 4 cut(s) 472, 958, 1153, 1293
BmsI GCATC 3 cut(s) 691, 824, 1384
BoxI GACNNNNGTC 1 cut(s) 723
BpiI GAAGAC 2 cut(s) 425, 502
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 2 cut(s) 534, 949
Bpu10I CCTNAGC 1 cut(s) 1066
BpuEI CTTGAG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 1253
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 4 cut(s) 61, 580, 795, 912
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 823
Bse1I ACTGG 1 cut(s) 1144
Bse3DI GCAATG 1 cut(s) 271
BseDI CCNNGG 4 cut(s) 61, 580, 795, 912
BseGI GGATG 6 cut(s) 460, 568, 728, 812, 886, 1013
BseLI CCNNNNNNNGG 2 cut(s) 467, 823
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 3 cut(s) 60, 1080, 1454
BseNI ACTGG 1 cut(s) 1144
BseRI GAGGAG 5 cut(s) 347, 588, 949, 952, 1356
BseX3I CGGCCG 1 cut(s) 1317
BseXI GCAGC 5 cut(s) 139, 904, 947, 950, 1264
Bsh1236I CGCG 4 cut(s) 777, 839, 1160, 1322
Bsh1285I CGRYCG 3 cut(s) 201, 1115, 1320
BshFI GGCC 4 cut(s) 527, 800, 1236, 1319
BsiEI CGRYCG 3 cut(s) 201, 1115, 1320
BsiSI CCGG 2 cut(s) 104, 524
BsiWI CGTACG 1 cut(s) 867
BslFI GGGAC 1 cut(s) 1137
BslI CCNNNNNNNGG 2 cut(s) 467, 823
BsmAI GTCTC 2 cut(s) 105, 552
BsmFI GGGAC 1 cut(s) 1137
BsnI GGCC 4 cut(s) 527, 800, 1236, 1319
Bso31I GGTCTC 1 cut(s) 552
Bsp19I CCATGG 2 cut(s) 795, 912
BspANI GGCC 4 cut(s) 527, 800, 1236, 1319
BspCNI CTCAG 3 cut(s) 59, 1079, 1453
BspFNI CGCG 4 cut(s) 777, 839, 1160, 1322
BspHI TCATGA 2 cut(s) 628, 649
BspLI GGNNCC 4 cut(s) 472, 958, 1153, 1293
BspPI GGATC 5 cut(s) 90, 214, 592, 1286, 1299
BspTNI GGTCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 271
BsrI ACTGG 1 cut(s) 1144
BssECI CCNNGG 4 cut(s) 61, 580, 795, 912
BssSI CACGAG 2 cut(s) 119, 329
BssT1I CCWWGG 2 cut(s) 795, 912
Bst2BI CACGAG 2 cut(s) 119, 329
Bst4CI ACNGT 3 cut(s) 546, 712, 743
Bst6I CTCTTC 2 cut(s) 391, 897
BstBAI YACGTR 1 cut(s) 1253
BstDEI CTNAG 5 cut(s) 46, 1066, 1090, 1139, 1440
BstDSI CCRYGG 3 cut(s) 61, 795, 912
BstF5I GGATG 6 cut(s) 460, 568, 728, 812, 886, 1013
BstFNI CGCG 4 cut(s) 777, 839, 1160, 1322
BstHHI GCGC 2 cut(s) 779, 839
BstMAI GTCTC 2 cut(s) 105, 552
BstMCI CGRYCG 3 cut(s) 201, 1115, 1320
BstMWI GCNNNNNNNGC 8 cut(s) 270, 322, 917, 938, 1123, 1283, 1313, 1316
BstPAI GACNNNNGTC 1 cut(s) 723
BstSFI CTRYAG 1 cut(s) 664
BstUI CGCG 4 cut(s) 777, 839, 1160, 1322
BstV1I GCAGC 5 cut(s) 139, 904, 947, 950, 1264
BstV2I GAAGAC 2 cut(s) 425, 502
BstX2I RGATCY 2 cut(s) 695, 1291
BstXI CCANNNNNNTGG 1 cut(s) 913
BstYI RGATCY 2 cut(s) 695, 1291
BstZI CGGCCG 1 cut(s) 1317
BsuI GTATCC 1 cut(s) 838
BsuRI GGCC 4 cut(s) 527, 800, 1236, 1319
BtgI CCRYGG 3 cut(s) 61, 795, 912
BtgZI GCGATG 2 cut(s) 288, 1301
BtrI CACGTC 2 cut(s) 559, 1005
BtsCI GGATG 6 cut(s) 460, 568, 728, 812, 886, 1013
BtsIMutI CAGTG 1 cut(s) 376
CciI TCATGA 2 cut(s) 628, 649
CciNI GCGGCCGC 1 cut(s) 1317
CfoI GCGC 2 cut(s) 779, 839
Cfr13I GGNCC 3 cut(s) 826, 1151, 1235
Csp6I GTAC 3 cut(s) 458, 621, 868
CviQI GTAC 3 cut(s) 458, 621, 868
DdeI CTNAG 5 cut(s) 46, 1066, 1090, 1139, 1440
DraIII CACNNNGTG 1 cut(s) 1253
EaeI YGGCCR 3 cut(s) 525, 798, 1317
EagI CGGCCG 1 cut(s) 1317
Eam1104I CTCTTC 2 cut(s) 391, 897
EarI CTCTTC 2 cut(s) 391, 897
EciI GGCGGA 2 cut(s) 919, 988
EclXI CGGCCG 1 cut(s) 1317
Eco130I CCWWGG 2 cut(s) 795, 912
Eco31I GGTCTC 1 cut(s) 552
Eco47I GGWCC 2 cut(s) 826, 1151
Eco52I CGGCCG 1 cut(s) 1317
Eco57I CTGAAG 2 cut(s) 326, 368
Eco72I CACGTG 1 cut(s) 1253
EcoT14I CCWWGG 2 cut(s) 795, 912
ErhI CCWWGG 2 cut(s) 795, 912
FaqI GGGAC 1 cut(s) 1137
FauI CCCGC 1 cut(s) 1162
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 301
FokI GGATG 6 cut(s) 467, 575, 735, 819, 873, 1000
FspBI CTAG 5 cut(s) 317, 485, 675, 693, 990
GlaI GCGC 2 cut(s) 778, 838
GsuI CTGGAG 2 cut(s) 534, 949
HaeIII GGCC 4 cut(s) 527, 800, 1236, 1319
HapII CCGG 2 cut(s) 104, 524
HhaI GCGC 2 cut(s) 779, 839
Hin6I GCGC 2 cut(s) 777, 837
HinP1I GCGC 2 cut(s) 777, 837
HinfI GANTC 8 cut(s) 68, 74, 98, 122, 337, 763, 1265, 1424
HpaII CCGG 2 cut(s) 104, 524
HphI GGTGA 2 cut(s) 579, 1090
Hpy166II GTNNAC 2 cut(s) 302, 821
Hpy188I TCNGA 5 cut(s) 73, 700, 747, 1296, 1340
Hpy8I GTNNAC 2 cut(s) 302, 821
Hpy99I CGWCG 3 cut(s) 199, 546, 560
HpyAV CCTTC 3 cut(s) 422, 866, 1027
HpyCH4III ACNGT 3 cut(s) 546, 712, 743
HpyCH4IV ACGT 5 cut(s) 112, 558, 866, 1004, 1252
HpyCH4V TGCA 5 cut(s) 577, 792, 920, 1397, 1449
HpyF10VI GCNNNNNNNGC 8 cut(s) 270, 322, 917, 938, 1123, 1283, 1313, 1316
HpyF3I CTNAG 5 cut(s) 46, 1066, 1090, 1139, 1440
HpySE526I ACGT 5 cut(s) 112, 558, 866, 1004, 1252
HspAI GCGC 2 cut(s) 777, 837
Ksp22I TGATCA 1 cut(s) 652
LmnI GCTCC 3 cut(s) 313, 962, 1075
Lsp1109I GCAGC 5 cut(s) 139, 904, 947, 950, 1264
LweI GCATC 3 cut(s) 691, 824, 1384
MaeI CTAG 5 cut(s) 317, 485, 675, 693, 990
MaeII ACGT 5 cut(s) 112, 558, 866, 1004, 1252
MaeIII GTNAC 2 cut(s) 377, 1415
MboII GAAGA 7 cut(s) 17, 139, 408, 425, 507, 644, 884
MflI RGATCY 2 cut(s) 695, 1291
MluCI AATT 7 cut(s) 30, 53, 241, 294, 656, 1182, 1225
MlyI GAGTC 3 cut(s) 68, 107, 131
MmeI TCCRAC 4 cut(s) 184, 291, 784, 853
MroXI GAANNNNTTC 2 cut(s) 12, 432
MseI TTAA 3 cut(s) 219, 1217, 1377
MslI CAYNNNNRTG 2 cut(s) 453, 911
MspI CCGG 2 cut(s) 104, 524
MvnI CGCG 4 cut(s) 777, 839, 1160, 1322
MwoI GCNNNNNNNGC 8 cut(s) 270, 322, 917, 938, 1123, 1283, 1313, 1316
NcoI CCATGG 2 cut(s) 795, 912
NlaIV GGNNCC 4 cut(s) 472, 958, 1153, 1293
NotI GCGGCCGC 1 cut(s) 1317
PagI TCATGA 2 cut(s) 628, 649
PdmI GAANNNNTTC 2 cut(s) 12, 432
PfeI GAWTC 5 cut(s) 68, 337, 763, 1265, 1424
Pfl23II CGTACG 1 cut(s) 867
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 3 cut(s) 68, 106, 130
PmaCI CACGTG 1 cut(s) 1253
PmlI CACGTG 1 cut(s) 1253
PpsI GAGTC 3 cut(s) 68, 106, 130
Ppu21I YACGTR 1 cut(s) 1253
PshAI GACNNNNGTC 1 cut(s) 723
PshBI ATTAAT 2 cut(s) 1217, 1377
PspCI CACGTG 1 cut(s) 1253
PspLI CGTACG 1 cut(s) 867
PspN4I GGNNCC 4 cut(s) 472, 958, 1153, 1293
PspPI GGNCC 3 cut(s) 826, 1151, 1235
PsuI RGATCY 2 cut(s) 695, 1291
PvuI CGATCG 1 cut(s) 201
RsaI GTAC 3 cut(s) 459, 622, 869
RsaNI GTAC 3 cut(s) 458, 621, 868
RseI CAYNNNNRTG 2 cut(s) 453, 911
SaqAI TTAA 3 cut(s) 219, 1217, 1377
Sau96I GGNCC 3 cut(s) 826, 1151, 1235
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 3 cut(s) 68, 107, 131
SfaNI GCATC 3 cut(s) 691, 824, 1384
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 2 cut(s) 826, 1151
SmiMI CAYNNNNRTG 2 cut(s) 453, 911
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 7 cut(s) 30, 53, 241, 294, 656, 1182, 1225
SspMI CTAG 5 cut(s) 317, 485, 675, 693, 990
StyI CCWWGG 2 cut(s) 795, 912
TaaI ACNGT 3 cut(s) 546, 712, 743
TaiI ACGT 5 cut(s) 115, 561, 869, 1007, 1255
TaqI TCGA 3 cut(s) 601, 1111, 1263
TasI AATT 7 cut(s) 30, 53, 241, 294, 656, 1182, 1225
TatI WGTACW 1 cut(s) 620
TauI GCSGC 5 cut(s) 935, 1160, 1310, 1319, 1322
TfiI GAWTC 5 cut(s) 68, 337, 763, 1265, 1424
Tru1I TTAA 3 cut(s) 219, 1217, 1377
Tru9I TTAA 3 cut(s) 219, 1217, 1377
TscAI CASTG 1 cut(s) 376
TseI GCWGC 5 cut(s) 152, 917, 935, 938, 1277
TspDTI ATGAA 6 cut(s) 234, 425, 524, 531, 645, 1208
TspGWI ACGGA 5 cut(s) 78, 673, 735, 806, 1434
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 2 cut(s) 826, 1151
VspI ATTAAT 2 cut(s) 1217, 1377
XapI RAATTY 2 cut(s) 53, 241
XbaI TCTAGA 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 301
XmnI GAANNNNTTC 2 cut(s) 12, 432
XspI CTAG 5 cut(s) 317, 485, 675, 693, 990
ZrmI AGTACT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.