Rw0G009910
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00405
OG0002724
Physical Location & Seq
Forward (+)
8634 .. 12092
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009910.1

Sequence Viewer

Length: 1206 bp
ATGGTGAACAAAATTGTTCTGACCACTCTTCAGCTTCAAGTTGAGTTACAGCGAGAACACGAAGAGAATCAAAAGCTCAGAACCATATTGGACCAAATCACCAAAAACTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTACAAGAGCAGCTGGGTTTGGAAGTATTGTTAATAGAGTTGGATGTTTTGTCAAAAAAACTTAACGATTATGGTGTGAAGCAAACTTGTGCAGAAGACAGCAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCAATCCCCCAAAAGTAGTTCTCATCATGATCATGATCAAGAACAAGTTCCAGCTGTTACTGATCATGATCAGGTTCCTTTAAGAAGGGCCAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGCGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGGTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGATTGGCAGAGGACAAGACTATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCAGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCTCAATCACACTGGACATGACCCGCACCCCTAACCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTTATAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGATCGACCCCACGGTTCGATGGTTGAGACAATAACTTCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCCTCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACAAGGAAGTACCAATGATATTGGTTCCCTTAACAGTAATGGGAGTGAGGTTTGCGCTGCATTTTCTGGCTCCCCACAGCTTCCTCAGTCCTGCACCACTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

401

Amino Acids

43.83

Weight (kDa)

6.78

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 139 - 196 1.8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 894
AccBSI CCGCTC 1 cut(s) 724
AciI CCGC 4 cut(s) 722, 793, 884, 947
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 2 cut(s) 450, 741
AcsI RAATTY 1 cut(s) 910
AcuI CTGAAG 3 cut(s) 14, 701, 984
AfaI GTAC 2 cut(s) 384, 1102
AfeI AGCGCT 1 cut(s) 936
AfiI CCNNNNNNNGG 3 cut(s) 728, 869, 890
AgsI TTSAA 4 cut(s) 38, 657, 753, 910
AjnI CCWGG 1 cut(s) 371
AloI GAACNNNNNNTCC 2 cut(s) 495, 527
Alw26I GTCTC 2 cut(s) 382, 983
AlwI GGATC 1 cut(s) 248
AlwNI CAGNNNCTG 1 cut(s) 599
Aor51HI AGCGCT 1 cut(s) 936
AoxI GGCC 3 cut(s) 369, 450, 741
ApoI RAATTY 1 cut(s) 910
AseI ATTAAT 1 cut(s) 1080
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 3 cut(s) 135, 937, 1148
AspS9I GGNCC 2 cut(s) 91, 369
AsuHPI GGTGA 2 cut(s) 16, 91
AvaII GGWCC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 897
BalI TGGCCA 2 cut(s) 452, 743
BanI GGYRCC 1 cut(s) 894
BbsI GAAGAC 1 cut(s) 246
BbvCI CCTCAGC 1 cut(s) 918
BccI CCATC 3 cut(s) 410, 442, 976
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 1 cut(s) 373
BclI TGATCA 5 cut(s) 138, 310, 316, 343, 349
BcoDI GTCTC 2 cut(s) 382, 983
BfaI CTAG 2 cut(s) 959, 1037
BfmI CTRYAG 2 cut(s) 696, 1029
BfoI RGCGCY 1 cut(s) 938
BlpI GCTNAGC 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 373
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 91, 369
BmiI GGNNCC 4 cut(s) 357, 896, 1117, 1162
BmrFI CCNGG 1 cut(s) 373
BmsI GCATC 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 1036
BsaBI GATNNNNATC 2 cut(s) 315, 348
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 372, 453, 738, 973
Bsc4I CCNNNNNNNGG 3 cut(s) 728, 869, 890
Bse1I ACTGG 1 cut(s) 786
Bse3DI GCAATG 1 cut(s) 957
Bse8I GATNNNNATC 2 cut(s) 315, 348
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 4 cut(s) 372, 453, 738, 973
BseGI GGATG 5 cut(s) 193, 425, 509, 734, 880
BseJI GATNNNNATC 2 cut(s) 315, 348
BseLI CCNNNNNNNGG 3 cut(s) 728, 869, 890
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 4 cut(s) 91, 140, 932, 1190
BseNI ACTGG 1 cut(s) 786
BseSI GKGCMC 1 cut(s) 897
BseYI CCCAGC 1 cut(s) 157
BsgI GTGCAG 4 cut(s) 255, 472, 744, 1168
BshFI GGCC 3 cut(s) 371, 452, 743
BshNI GGYRCC 1 cut(s) 894
BsiWI CGTACG 1 cut(s) 382
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 3 cut(s) 728, 869, 890
BsmAI GTCTC 2 cut(s) 382, 983
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 3 cut(s) 371, 452, 743
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 4 cut(s) 722, 793, 884, 947
BspANI GGCC 3 cut(s) 371, 452, 743
BspCNI CTCAG 4 cut(s) 90, 139, 931, 1189
BspHI TCATGA 3 cut(s) 307, 313, 346
BspLI GGNNCC 4 cut(s) 357, 896, 1117, 1162
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 894
BspTNI GGTCTC 1 cut(s) 382
BsrBI CCGCTC 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 1 cut(s) 786
BssECI CCNNGG 4 cut(s) 372, 453, 738, 973
BssMI GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
BssT1I CCWWGG 2 cut(s) 453, 738
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 4 cut(s) 440, 842, 977, 1127
Bst6I CTCTTC 5 cut(s) 33, 57, 594, 715, 720
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 4 cut(s) 77, 126, 918, 1176
BstDSI CCRYGG 2 cut(s) 738, 973
BstF5I GGATG 5 cut(s) 193, 425, 509, 734, 880
BstH2I RGCGCY 1 cut(s) 938
BstHHI GCGC 3 cut(s) 135, 937, 1148
BstKTI GATC 8 cut(s) 141, 256, 313, 319, 346, 352, 395, 967
BstMAI GTCTC 2 cut(s) 382, 983
BstMBI GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
BstMWI GCNNNNNNNGC 4 cut(s) 127, 635, 892, 1040
BstNI CCWGG 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 371
BstSFI CTRYAG 2 cut(s) 696, 1029
BstSLI GKGCMC 1 cut(s) 897
BstV2I GAAGAC 1 cut(s) 246
BsuRI GGCC 3 cut(s) 371, 452, 743
BtgI CCRYGG 2 cut(s) 738, 973
BtgZI GCGATG 1 cut(s) 429
BtsCI GGATG 5 cut(s) 193, 425, 509, 734, 880
BtsIMutI CAGTG 1 cut(s) 779
Cac8I GCNNGC 1 cut(s) 123
CaiI CAGNNNCTG 1 cut(s) 599
CciI TCATGA 3 cut(s) 307, 313, 346
CfoI GCGC 3 cut(s) 135, 937, 1148
Cfr13I GGNCC 2 cut(s) 91, 369
Csp6I GTAC 2 cut(s) 383, 1101
CviQI GTAC 2 cut(s) 383, 1101
DdeI CTNAG 4 cut(s) 77, 126, 918, 1176
DpnI GATC 8 cut(s) 140, 255, 312, 318, 345, 351, 394, 966
DpnII GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
EaeI YGGCCR 2 cut(s) 450, 741
Eam1104I CTCTTC 5 cut(s) 33, 57, 594, 715, 720
EarI CTCTTC 5 cut(s) 33, 57, 594, 715, 720
Eco130I CCWWGG 2 cut(s) 453, 738
Eco31I GGTCTC 1 cut(s) 382
Eco32I GATATC 2 cut(s) 606, 648
Eco47I GGWCC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 936
Eco57I CTGAAG 3 cut(s) 14, 701, 984
EcoRII CCWGG 1 cut(s) 371
EcoRV GATATC 2 cut(s) 606, 648
EcoT14I CCWWGG 2 cut(s) 453, 738
ErhI CCWWGG 2 cut(s) 453, 738
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 1 cut(s) 453
FauI CCCGC 1 cut(s) 800
FauNDI CATATG 1 cut(s) 565
FbaI TGATCA 5 cut(s) 138, 310, 316, 343, 349
FokI GGATG 5 cut(s) 200, 432, 516, 721, 867
FspBI CTAG 2 cut(s) 959, 1037
FspI TGCGCA 1 cut(s) 134
GlaI GCGC 3 cut(s) 134, 936, 1147
GsaI CCCAGC 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 938
HaeIII GGCC 3 cut(s) 371, 452, 743
HhaI GCGC 3 cut(s) 135, 937, 1148
Hin6I GCGC 3 cut(s) 133, 935, 1146
HinP1I GCGC 3 cut(s) 133, 935, 1146
HinfI GANTC 1 cut(s) 67
HphI GGTGA 2 cut(s) 16, 91
Hpy166II GTNNAC 2 cut(s) 7, 1026
Hpy188I TCNGA 8 cut(s) 21, 80, 143, 258, 397, 516, 555, 1015
Hpy188III TCNNGA 5 cut(s) 251, 308, 314, 320, 347
Hpy8I GTNNAC 2 cut(s) 7, 1026
HpyAV CCTTC 3 cut(s) 277, 360, 563
HpyCH4III ACNGT 4 cut(s) 440, 842, 977, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 635, 892, 1040
HpyF3I CTNAG 4 cut(s) 77, 126, 918, 1176
HspAI GCGC 3 cut(s) 133, 935, 1146
Ksp22I TGATCA 5 cut(s) 138, 310, 316, 343, 349
Kzo9I GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
LmnI GCTCC 2 cut(s) 406, 1166
LweI GCATC 1 cut(s) 494
MaeI CTAG 2 cut(s) 959, 1037
MaeIII GTNAC 2 cut(s) 45, 337
MalI GATC 8 cut(s) 140, 255, 312, 318, 345, 351, 394, 966
MbiI CCGCTC 1 cut(s) 724
MboI GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
MboII GAAGA 6 cut(s) 20, 74, 251, 581, 702, 707
MhlI GDGCHC 1 cut(s) 897
MlsI TGGCCA 2 cut(s) 452, 743
MluCI AATT 2 cut(s) 12, 910
MluNI TGGCCA 2 cut(s) 452, 743
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 9 cut(s) 423, 532, 597, 766, 781, 927, 1058, 1132, 1185
Mox20I TGGCCA 2 cut(s) 452, 743
MroXI GAANNNNTTC 1 cut(s) 327
MscI TGGCCA 2 cut(s) 452, 743
MseI TTAA 5 cut(s) 176, 207, 362, 1080, 1122
MslI CAYNNNNRTG 2 cut(s) 312, 927
Msp20I TGGCCA 2 cut(s) 452, 743
MspA1I CMGCKG 5 cut(s) 130, 157, 335, 599, 947
MspR9I CCNGG 1 cut(s) 373
MvaI CCWGG 1 cut(s) 373
MwoI GCNNNNNNNGC 4 cut(s) 127, 635, 892, 1040
NcoI CCATGG 1 cut(s) 738
NdeI CATATG 1 cut(s) 565
NdeII GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
NlaIV GGNNCC 4 cut(s) 357, 896, 1117, 1162
NsbI TGCGCA 1 cut(s) 134
PagI TCATGA 3 cut(s) 307, 313, 346
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 67
Pfl23II CGTACG 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 1080
Psp6I CCWGG 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 371
PspLI CGTACG 1 cut(s) 382
PspN4I GGNNCC 4 cut(s) 357, 896, 1117, 1162
PspPI GGNCC 2 cut(s) 91, 369
PstNI CAGNNNCTG 1 cut(s) 599
PvuII CAGCTG 4 cut(s) 130, 157, 335, 599
RsaI GTAC 2 cut(s) 384, 1102
RsaNI GTAC 2 cut(s) 383, 1101
RseI CAYNNNNRTG 2 cut(s) 312, 927
SaqAI TTAA 5 cut(s) 176, 207, 362, 1080, 1122
Sau3AI GATC 8 cut(s) 138, 253, 310, 316, 343, 349, 392, 964
Sau96I GGNCC 2 cut(s) 91, 369
ScrFI CCNGG 1 cut(s) 373
SduI GDGCHC 1 cut(s) 897
SfaNI GCATC 1 cut(s) 494
SfcI CTRYAG 2 cut(s) 696, 1029
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 2 cut(s) 312, 927
SmlI CTYRAG 1 cut(s) 1051
SmoI CTYRAG 1 cut(s) 1051
Sse9I AATT 2 cut(s) 12, 910
SsiI CCGC 4 cut(s) 722, 793, 884, 947
SspI AATATT 1 cut(s) 1078
SspMI CTAG 2 cut(s) 959, 1037
StyD4I CCNGG 1 cut(s) 371
StyI CCWWGG 2 cut(s) 453, 738
TaaI ACNGT 4 cut(s) 440, 842, 977, 1127
TaqI TCGA 2 cut(s) 967, 980
TasI AATT 2 cut(s) 12, 910
TauI GCSGC 2 cut(s) 724, 950
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 5 cut(s) 176, 207, 362, 1080, 1122
Tru9I TTAA 5 cut(s) 176, 207, 362, 1080, 1122
TscAI CASTG 1 cut(s) 786
TspDTI ATGAA 4 cut(s) 582, 723, 823, 1076
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 91
VspI ATTAAT 1 cut(s) 1080
XapI RAATTY 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 959, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.