RchiOBHm_Chr3g0485711
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002724
Physical Location & Seq
Forward (+)
32490850 .. 32493560
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45030

Sequence Viewer

Length: 1344 bp
ATGGATGAACAACGCTGTGACTTGAAATACTTGCACTCGGACCAGAGCTTTCTGGGGCTCAGTACTAGTTCCGATCATCGTCTAGTTGAACCCCCAATAAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCTTTTCCAATAATAGACTTCCCGATCAACCGGCGCCTGGTCATGCCGAGCTGACCACTCTTCAGCTTCAAGTTGAGTTACAGCGAGAATACGAAGAGAATCAAAAGCTCAGAACCATGTTGGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTTCAAGAGCAGCTGCAAATTTGTGCAGAAGACAGCATTAATCTTGATCCGAAGTTATCCTTCCTACATGATGATCAATACCCCAAAAGTAGTTCTCATCACGATCATGATCAAGAACAAGTTCTTGCTGCTACTGATCATGATCAGGTTCCTTCAAGAAGGGCTAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGAGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGTTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGAATGGCAGAGGACAAGACCATTCTGATAACAACGTATGAAGGTAATCACAACCACCCTCTTCCTGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCCGCACCCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTAATAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGAACAACCCCACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCATCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACATGGAAATACCAATGGTATTAGTTCCCTTAACAGTAATGGGAATGGGGTTGCGCAGCATTATCTGGCTCCCCACAGCTTCCTCAATCCTGTACCACTTTCTCCACCACCTAATCCTGCATGCATGATGCATGGGGTATATATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

447

Amino Acids

49.49

Weight (kDa)

6.44

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 175 - 232 2.4e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 305, 1248
AccB1I GGYRCC 2 cut(s) 172, 996
AccBSI CCGCTC 1 cut(s) 832
AciI CCGC 4 cut(s) 830, 901, 986, 1049
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 2 cut(s) 558, 849
AcsI RAATTY 2 cut(s) 333, 1012
AcuI CTGAAG 2 cut(s) 185, 809
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 3 cut(s) 64, 492, 1287
AfeI AGCGCT 1 cut(s) 1038
AfiI CCNNNNNNNGG 4 cut(s) 176, 836, 971, 992
AgsI TTSAA 9 cut(s) 25, 89, 118, 209, 320, 471, 765, 861, 1012
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 603, 635
Alw26I GTCTC 2 cut(s) 490, 1085
AlwI GGATC 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 1038
AoxI GGCC 2 cut(s) 558, 849
ApoI RAATTY 2 cut(s) 333, 1012
AseI ATTAAT 2 cut(s) 354, 1182
Asp700I GAANNNNTTC 1 cut(s) 435
AspLEI GCGC 4 cut(s) 175, 306, 1039, 1249
AspS9I GGNCC 2 cut(s) 40, 262
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 40, 262
BaeGI GKGCMC 1 cut(s) 999
BalI TGGCCA 2 cut(s) 560, 851
BanI GGYRCC 2 cut(s) 172, 996
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1020
BccI CCATC 4 cut(s) 518, 550, 1078, 1157
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 5 cut(s) 309, 388, 424, 451, 457
BcoDI GTCTC 2 cut(s) 490, 1085
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 5 cut(s) 66, 83, 480, 1061, 1139
BfmI CTRYAG 4 cut(s) 702, 804, 1101, 1131
BfoI RGCGCY 2 cut(s) 176, 1040
BlpI GCTNAGC 1 cut(s) 297
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 40, 262
BmgT120I GGNCC 2 cut(s) 40, 262
BmiI GGNNCC 4 cut(s) 174, 465, 998, 1263
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 602, 1311
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 1138
BsaBI GATNNNNATC 2 cut(s) 423, 456
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 3 cut(s) 561, 846, 1075
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 836, 971, 992
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 894
Bse3DI GCAATG 1 cut(s) 1059
Bse8I GATNNNNATC 2 cut(s) 423, 456
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 561, 846, 1075
BseGI GGATG 5 cut(s) 10, 533, 617, 842, 982
BseJI GATNNNNATC 2 cut(s) 423, 456
BseLI CCNNNNNNNGG 4 cut(s) 176, 836, 971, 992
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 4 cut(s) 73, 262, 311, 1034
BseNI ACTGG 1 cut(s) 894
BseSI GKGCMC 1 cut(s) 999
BsgI GTGCAG 3 cut(s) 360, 580, 852
BshFI GGCC 2 cut(s) 560, 851
BshNI GGYRCC 2 cut(s) 172, 996
BsiSI CCGG 1 cut(s) 170
BsiWI CGTACG 1 cut(s) 490
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 4 cut(s) 176, 836, 971, 992
BsmAI GTCTC 2 cut(s) 490, 1085
BsmFI GGGAC 1 cut(s) 561
BsnI GGCC 2 cut(s) 560, 851
Bso31I GGTCTC 1 cut(s) 490
Bsp1286I GDGCHC 2 cut(s) 60, 999
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 4 cut(s) 830, 901, 986, 1049
BspANI GGCC 2 cut(s) 560, 851
BspCNI CTCAG 4 cut(s) 72, 261, 310, 1033
BspHI TCATGA 2 cut(s) 421, 454
BspLI GGNNCC 4 cut(s) 174, 465, 998, 1263
BspMAI CTGCAG 2 cut(s) 706, 1105
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 172, 996
BspTNI GGTCTC 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 832
BsrDI GCAATG 1 cut(s) 1059
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 894
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 561, 846, 1075
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 561, 846
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 4 cut(s) 548, 944, 1079, 1229
Bst6I CTCTTC 5 cut(s) 204, 228, 702, 823, 828
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 294, 1315
BstDEI CTNAG 4 cut(s) 59, 248, 297, 1020
BstDSI CCRYGG 2 cut(s) 846, 1075
BstF5I GGATG 5 cut(s) 10, 533, 617, 842, 982
BstH2I RGCGCY 2 cut(s) 176, 1040
BstHHI GCGC 4 cut(s) 175, 306, 1039, 1249
BstMAI GTCTC 2 cut(s) 490, 1085
BstMWI GCNNNNNNNGC 4 cut(s) 298, 743, 994, 1142
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 1317
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 4 cut(s) 702, 804, 1101, 1131
BstSLI GKGCMC 1 cut(s) 999
BstV2I GAAGAC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 560, 851
BtgI CCRYGG 2 cut(s) 846, 1075
BtsCI GGATG 5 cut(s) 10, 533, 617, 842, 982
BtsIMutI CAGTG 1 cut(s) 887
Cac8I GCNNGC 2 cut(s) 294, 1315
CciI TCATGA 2 cut(s) 421, 454
CfoI GCGC 4 cut(s) 175, 306, 1039, 1249
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 40, 262
Csp6I GTAC 3 cut(s) 63, 491, 1286
CviQI GTAC 3 cut(s) 63, 491, 1286
DdeI CTNAG 4 cut(s) 59, 248, 297, 1020
DinI GGCGCC 1 cut(s) 174
EaeI YGGCCR 2 cut(s) 558, 849
Eam1104I CTCTTC 5 cut(s) 204, 228, 702, 823, 828
EarI CTCTTC 5 cut(s) 204, 228, 702, 823, 828
Eco130I CCWWGG 2 cut(s) 561, 846
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 490
Eco32I GATATC 2 cut(s) 714, 756
Eco47I GGWCC 2 cut(s) 40, 262
Eco47III AGCGCT 1 cut(s) 1038
Eco57I CTGAAG 2 cut(s) 185, 809
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 2 cut(s) 714, 756
EcoT14I CCWWGG 2 cut(s) 561, 846
EcoT22I ATGCAT 2 cut(s) 1319, 1326
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 561, 846
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 1 cut(s) 561
FauI CCCGC 1 cut(s) 908
FbaI TGATCA 5 cut(s) 309, 388, 424, 451, 457
FokI GGATG 5 cut(s) 17, 540, 624, 829, 969
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 5 cut(s) 66, 83, 480, 1061, 1139
FspI TGCGCA 2 cut(s) 305, 1248
GlaI GCGC 4 cut(s) 174, 305, 1038, 1248
HaeII RGCGCY 2 cut(s) 176, 1040
HaeIII GGCC 2 cut(s) 560, 851
HapII CCGG 1 cut(s) 170
HhaI GCGC 4 cut(s) 175, 306, 1039, 1249
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 4 cut(s) 173, 304, 1037, 1247
HinP1I GCGC 4 cut(s) 173, 304, 1037, 1247
HinfI GANTC 1 cut(s) 238
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 1128
Hpy188I TCNGA 9 cut(s) 40, 73, 251, 314, 366, 505, 624, 663, 1117
Hpy188III TCNNGA 8 cut(s) 161, 320, 359, 416, 422, 428, 455, 471
Hpy8I GTNNAC 1 cut(s) 1128
HpyAV CCTTC 4 cut(s) 385, 468, 477, 671
HpyCH4III ACNGT 4 cut(s) 548, 944, 1079, 1229
HpyCH4IV ACGT 1 cut(s) 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 298, 743, 994, 1142
HpyF3I CTNAG 4 cut(s) 59, 248, 297, 1020
HpySE526I ACGT 1 cut(s) 671
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 4 cut(s) 173, 304, 1037, 1247
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 5 cut(s) 309, 388, 424, 451, 457
LmnI GCTCC 2 cut(s) 514, 1267
LweI GCATC 2 cut(s) 602, 1311
MaeI CTAG 5 cut(s) 66, 83, 480, 1061, 1139
MaeII ACGT 1 cut(s) 671
MaeIII GTNAC 2 cut(s) 17, 216
MbiI CCGCTC 1 cut(s) 832
MboII GAAGA 7 cut(s) 116, 191, 245, 356, 689, 810, 815
MhlI GDGCHC 2 cut(s) 60, 999
MlsI TGGCCA 2 cut(s) 560, 851
MluCI AATT 2 cut(s) 333, 1012
MluNI TGGCCA 2 cut(s) 560, 851
Mly113I GGCGCC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 6 cut(s) 531, 640, 705, 874, 1029, 1286
Mox20I TGGCCA 2 cut(s) 560, 851
Mph1103I ATGCAT 2 cut(s) 1319, 1326
MroXI GAANNNNTTC 1 cut(s) 435
MscI TGGCCA 2 cut(s) 560, 851
MseI TTAA 3 cut(s) 354, 1182, 1224
MslI CAYNNNNRTG 2 cut(s) 420, 1029
Msp20I TGGCCA 2 cut(s) 560, 851
MspA1I CMGCKG 4 cut(s) 301, 328, 707, 1049
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 298, 743, 994, 1142
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 846
NlaIV GGNNCC 4 cut(s) 174, 465, 998, 1263
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 2 cut(s) 305, 1248
NsiI ATGCAT 2 cut(s) 1319, 1326
NspI RCATGY 1 cut(s) 1317
PaeI GCATGC 1 cut(s) 1317
PagI TCATGA 2 cut(s) 421, 454
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 238
Pfl23II CGTACG 1 cut(s) 490
PluTI GGCGCC 1 cut(s) 176
PshBI ATTAAT 2 cut(s) 354, 1182
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 490
PspN4I GGNNCC 4 cut(s) 174, 465, 998, 1263
PspPI GGNCC 2 cut(s) 40, 262
PstI CTGCAG 2 cut(s) 706, 1105
PvuII CAGCTG 3 cut(s) 301, 328, 707
RsaI GTAC 3 cut(s) 64, 492, 1287
RsaNI GTAC 3 cut(s) 63, 491, 1286
RseI CAYNNNNRTG 2 cut(s) 420, 1029
SaqAI TTAA 3 cut(s) 354, 1182, 1224
Sau96I GGNCC 2 cut(s) 40, 262
ScaI AGTACT 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 60, 999
SfaNI GCATC 2 cut(s) 602, 1311
SfcI CTRYAG 4 cut(s) 702, 804, 1101, 1131
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 40, 262
SmiMI CAYNNNNRTG 2 cut(s) 420, 1029
SmlI CTYRAG 1 cut(s) 1153
SmoI CTYRAG 1 cut(s) 1153
SpeI ACTAGT 1 cut(s) 65
SphI GCATGC 1 cut(s) 1317
Sse9I AATT 2 cut(s) 333, 1012
SsiI CCGC 4 cut(s) 830, 901, 986, 1049
SspDI GGCGCC 1 cut(s) 172
SspI AATATT 1 cut(s) 1180
SspMI CTAG 5 cut(s) 66, 83, 480, 1061, 1139
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 2 cut(s) 561, 846
TaaI ACNGT 4 cut(s) 548, 944, 1079, 1229
TaiI ACGT 1 cut(s) 674
TaqI TCGA 1 cut(s) 1082
TasI AATT 2 cut(s) 333, 1012
TatI WGTACW 1 cut(s) 62
TauI GCSGC 2 cut(s) 832, 1052
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 3 cut(s) 354, 1182, 1224
Tru9I TTAA 3 cut(s) 354, 1182, 1224
TscAI CASTG 1 cut(s) 894
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 21, 690, 831, 925, 1178
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 1 cut(s) 894
VpaK11BI GGWCC 2 cut(s) 40, 262
VspI ATTAAT 2 cut(s) 354, 1182
XapI RAATTY 2 cut(s) 333, 1012
XceI RCATGY 1 cut(s) 1317
XcmI CCANNNNNNNNNTGG 2 cut(s) 886, 934
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 5 cut(s) 66, 83, 480, 1061, 1139
ZrmI AGTACT 1 cut(s) 64
Zsp2I ATGCAT 2 cut(s) 1319, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.