Rh3DG300200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002724
Physical Location & Seq
Forward (+)
31544096 .. 31547189
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG300200.1

Sequence Viewer

Length: 1311 bp
ATGGATGAACAACTCTGTGACTTGAAACACTTACACTCGGACCAGAGCTTTCTGGGGCTCAGTAGTAGTTCCGATCATCGTCTAGTTGAACCCCCAATAAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCCTTTCCAATAATAGACTTCCCAATCAACCGCCGCCTGGTCATGCAGAGCTGACCACTCTTCAGCTTCAAGTTGAGTTACAGCGAGAACACGAAGAGAATCAAAAGCTCAGAACCATATTGGACCAAATCACCAAAAACTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTACAAGAGCAGCTGCAAACTTGTGCAGAAGACAGCAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCAATCCCCCAAAAGTAGTTCTCATCATGATCATGATCAAGAACAAGTTCCAGCTGTTACTGATCATGATCAGGTTCCTTTAAGAAGGGCTAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGCGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGGTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGATTGGCAGAGGACAAGACTATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCAGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCTCAATCACACTGGACATGACCCGCACCCCTAACCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTTATAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGATCGACCCCACGGTTCGATGGTTGAGACAATAACTTCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCCTCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACAAGGAAGTACCAATGATATTGGTTCCCTTAACAGTAATGGGAGTGAGGTTTGCGCTGCATTTTCTGGCTCCCCACAGCTTCCTCAGTCCTGCACCACTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

436

Amino Acids

47.88

Weight (kDa)

6.22

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 174 - 231 2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 999
AccBSI CCGCTC 1 cut(s) 829
AciI CCGC 6 cut(s) 170, 173, 827, 898, 989, 1052
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 555, 846
AcsI RAATTY 1 cut(s) 1015
AcuI CTGAAG 3 cut(s) 185, 806, 1089
AfaI GTAC 2 cut(s) 489, 1207
AfeI AGCGCT 1 cut(s) 1041
AfiI CCNNNNNNNGG 4 cut(s) 176, 833, 974, 995
AgsI TTSAA 7 cut(s) 25, 89, 118, 209, 762, 858, 1015
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 600, 632
Alw26I GTCTC 2 cut(s) 487, 1088
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 704
Aor51HI AGCGCT 1 cut(s) 1041
AoxI GGCC 2 cut(s) 555, 846
ApoI RAATTY 1 cut(s) 1015
AseI ATTAAT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 3 cut(s) 306, 1042, 1253
AspS9I GGNCC 2 cut(s) 40, 262
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 40, 262
BaeGI GKGCMC 1 cut(s) 1002
BalI TGGCCA 2 cut(s) 557, 848
BanI GGYRCC 1 cut(s) 999
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1023
BccI CCATC 3 cut(s) 515, 547, 1081
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 5 cut(s) 309, 415, 421, 448, 454
BcoDI GTCTC 2 cut(s) 487, 1088
BfaI CTAG 4 cut(s) 83, 477, 1064, 1142
BfmI CTRYAG 2 cut(s) 801, 1134
BfoI RGCGCY 1 cut(s) 1043
BlpI GCTNAGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 40, 262
BmgT120I GGNCC 2 cut(s) 40, 262
BmiI GGNNCC 4 cut(s) 462, 1001, 1222, 1267
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 1141
BsaBI GATNNNNATC 2 cut(s) 420, 453
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 558, 843, 1078
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 833, 974, 995
Bse1I ACTGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 2 cut(s) 420, 453
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 558, 843, 1078
BseGI GGATG 5 cut(s) 10, 530, 614, 839, 985
BseJI GATNNNNATC 2 cut(s) 420, 453
BseLI CCNNNNNNNGG 4 cut(s) 176, 833, 974, 995
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 5 cut(s) 73, 262, 311, 1037, 1295
BseNI ACTGG 1 cut(s) 891
BseSI GKGCMC 1 cut(s) 1002
BsgI GTGCAG 4 cut(s) 360, 577, 849, 1273
BshFI GGCC 2 cut(s) 557, 848
BshNI GGYRCC 1 cut(s) 999
BsiWI CGTACG 1 cut(s) 487
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 4 cut(s) 176, 833, 974, 995
BsmAI GTCTC 2 cut(s) 487, 1088
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 2 cut(s) 557, 848
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 60, 1002
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 6 cut(s) 170, 173, 827, 898, 989, 1052
BspANI GGCC 2 cut(s) 557, 848
BspCNI CTCAG 5 cut(s) 72, 261, 310, 1036, 1294
BspHI TCATGA 3 cut(s) 412, 418, 451
BspLI GGNNCC 4 cut(s) 462, 1001, 1222, 1267
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 999
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 829
BsrDI GCAATG 1 cut(s) 1062
BsrI ACTGG 1 cut(s) 891
BssECI CCNNGG 3 cut(s) 558, 843, 1078
BssT1I CCWWGG 2 cut(s) 558, 843
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 4 cut(s) 545, 947, 1082, 1232
Bst6I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 5 cut(s) 59, 248, 297, 1023, 1281
BstDSI CCRYGG 2 cut(s) 843, 1078
BstF5I GGATG 5 cut(s) 10, 530, 614, 839, 985
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 3 cut(s) 306, 1042, 1253
BstMAI GTCTC 2 cut(s) 487, 1088
BstMWI GCNNNNNNNGC 4 cut(s) 298, 740, 997, 1145
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 2 cut(s) 801, 1134
BstSLI GKGCMC 1 cut(s) 1002
BstV2I GAAGAC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 557, 848
BtgI CCRYGG 2 cut(s) 843, 1078
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 5 cut(s) 10, 530, 614, 839, 985
BtsIMutI CAGTG 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 294
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 3 cut(s) 412, 418, 451
CfoI GCGC 3 cut(s) 306, 1042, 1253
Cfr13I GGNCC 2 cut(s) 40, 262
Csp6I GTAC 2 cut(s) 488, 1206
CviQI GTAC 2 cut(s) 488, 1206
DdeI CTNAG 5 cut(s) 59, 248, 297, 1023, 1281
EaeI YGGCCR 2 cut(s) 555, 846
Eam1104I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
EarI CTCTTC 5 cut(s) 204, 228, 699, 820, 825
Eco130I CCWWGG 2 cut(s) 558, 843
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 487
Eco32I GATATC 2 cut(s) 711, 753
Eco47I GGWCC 2 cut(s) 40, 262
Eco47III AGCGCT 1 cut(s) 1041
Eco57I CTGAAG 3 cut(s) 185, 806, 1089
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 2 cut(s) 711, 753
EcoT14I CCWWGG 2 cut(s) 558, 843
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 558, 843
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 558
FauI CCCGC 1 cut(s) 905
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 5 cut(s) 309, 415, 421, 448, 454
FokI GGATG 5 cut(s) 17, 537, 621, 826, 972
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 4 cut(s) 83, 477, 1064, 1142
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 3 cut(s) 305, 1041, 1252
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 2 cut(s) 557, 848
HhaI GCGC 3 cut(s) 306, 1042, 1253
Hin6I GCGC 3 cut(s) 304, 1040, 1251
HinP1I GCGC 3 cut(s) 304, 1040, 1251
HinfI GANTC 1 cut(s) 238
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 1131
Hpy188I TCNGA 9 cut(s) 40, 73, 251, 314, 363, 502, 621, 660, 1120
Hpy188III TCNNGA 5 cut(s) 356, 413, 419, 425, 452
Hpy8I GTNNAC 1 cut(s) 1131
HpyAV CCTTC 3 cut(s) 382, 465, 668
HpyCH4III ACNGT 4 cut(s) 545, 947, 1082, 1232
HpyF10VI GCNNNNNNNGC 4 cut(s) 298, 740, 997, 1145
HpyF3I CTNAG 5 cut(s) 59, 248, 297, 1023, 1281
HspAI GCGC 3 cut(s) 304, 1040, 1251
Ksp22I TGATCA 5 cut(s) 309, 415, 421, 448, 454
LmnI GCTCC 2 cut(s) 511, 1271
LweI GCATC 1 cut(s) 599
MaeI CTAG 4 cut(s) 83, 477, 1064, 1142
MaeIII GTNAC 3 cut(s) 17, 216, 442
MbiI CCGCTC 1 cut(s) 829
MboII GAAGA 7 cut(s) 116, 191, 245, 356, 686, 807, 812
MhlI GDGCHC 2 cut(s) 60, 1002
MlsI TGGCCA 2 cut(s) 557, 848
MluCI AATT 1 cut(s) 1015
MluNI TGGCCA 2 cut(s) 557, 848
MnlI CCTC 9 cut(s) 528, 637, 702, 871, 886, 1032, 1163, 1237, 1290
Mox20I TGGCCA 2 cut(s) 557, 848
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 2 cut(s) 557, 848
MseI TTAA 3 cut(s) 467, 1185, 1227
MslI CAYNNNNRTG 2 cut(s) 417, 1032
Msp20I TGGCCA 2 cut(s) 557, 848
MspA1I CMGCKG 5 cut(s) 301, 328, 440, 704, 1052
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 298, 740, 997, 1145
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 670
NlaIV GGNNCC 4 cut(s) 462, 1001, 1222, 1267
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 1 cut(s) 305
PagI TCATGA 3 cut(s) 412, 418, 451
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 1 cut(s) 238
Pfl23II CGTACG 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 1185
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 462, 1001, 1222, 1267
PspPI GGNCC 2 cut(s) 40, 262
PstNI CAGNNNCTG 1 cut(s) 704
PvuII CAGCTG 4 cut(s) 301, 328, 440, 704
RsaI GTAC 2 cut(s) 489, 1207
RsaNI GTAC 2 cut(s) 488, 1206
RseI CAYNNNNRTG 2 cut(s) 417, 1032
SaqAI TTAA 3 cut(s) 467, 1185, 1227
Sau96I GGNCC 2 cut(s) 40, 262
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 60, 1002
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 2 cut(s) 801, 1134
SinI GGWCC 2 cut(s) 40, 262
SmiMI CAYNNNNRTG 2 cut(s) 417, 1032
SmlI CTYRAG 1 cut(s) 1156
SmoI CTYRAG 1 cut(s) 1156
Sse9I AATT 1 cut(s) 1015
SsiI CCGC 6 cut(s) 170, 173, 827, 898, 989, 1052
SspI AATATT 1 cut(s) 1183
SspMI CTAG 4 cut(s) 83, 477, 1064, 1142
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 2 cut(s) 558, 843
TaaI ACNGT 4 cut(s) 545, 947, 1082, 1232
TaqI TCGA 2 cut(s) 1072, 1085
TasI AATT 1 cut(s) 1015
TauI GCSGC 3 cut(s) 175, 829, 1055
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 3 cut(s) 467, 1185, 1227
Tru9I TTAA 3 cut(s) 467, 1185, 1227
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 21, 687, 828, 928, 1181
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 1 cut(s) 891
VpaK11BI GGWCC 2 cut(s) 40, 262
VspI ATTAAT 1 cut(s) 1185
XapI RAATTY 1 cut(s) 1015
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 4 cut(s) 83, 477, 1064, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.