Rroxscaffold_6G00396500
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002724
Physical Location & Seq
Reverse (-)
18029847 .. 18032779
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396500.1

Sequence Viewer

Length: 1056 bp
ATGTTGGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTACAAGAGCAGCTGCAAACTTGTGCAGAAGACAGCAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCGATCCCCCAAAAGTAGTTCTCATCCCGATCATGATCAAGAACAAGTTCTAGCTGTTACTGATCATGATCAGGTTCCTTTAAGAAGGGCTAGGGTCTCCGTACTAGCAAGATCGGAAGCTCCCACGATTAGCGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGGTCGCTGGATGCCCCGTTCGGAAACAGGTACAAAGATTGGCAGAGGACAAGACCATTCTGATAACAAAATATGAAGGTAATCACAACCACCCTCTTCCTCCAGCTGCACTATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCGGCACCCCTAACCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTTACAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGATCGACCCCACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACATCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCCTCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACAAGGAAGTACCAATGATATTAGTTCCCTTAACAGTAATGGGAATGAGGTTTGCGCTGCATTTTCTGGCTCCCCACAGCTTCCTCAGTCCTGCACCACTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

351

Amino Acids

37.95

Weight (kDa)

6.94

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 89 - 146 1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 50
AccB1I GGYRCC 2 cut(s) 644, 744
AccBSI CCGCTC 1 cut(s) 574
AciI CCGC 3 cut(s) 572, 734, 797
AclWI GGATC 2 cut(s) 98, 129
AcoI YGGCCR 2 cut(s) 300, 591
AcsI RAATTY 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 3 cut(s) 234, 375, 952
AfeI AGCGCT 1 cut(s) 786
AfiI CCNNNNNNNGG 3 cut(s) 578, 719, 740
AgsI TTSAA 3 cut(s) 507, 603, 760
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 9 cut(s) 24, 46, 73, 185, 251, 449, 524, 890, 1021
AluI AGCT 9 cut(s) 24, 46, 73, 185, 251, 449, 524, 890, 1021
Alw26I GTCTC 2 cut(s) 232, 833
AlwI GGATC 2 cut(s) 98, 129
Aor51HI AGCGCT 1 cut(s) 786
AoxI GGCC 2 cut(s) 300, 591
ApoI RAATTY 1 cut(s) 760
AseI ATTAAT 1 cut(s) 930
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 3 cut(s) 51, 787, 998
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 7
AvaII GGWCC 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 747
BalI TGGCCA 2 cut(s) 302, 593
BanI GGYRCC 2 cut(s) 644, 744
BbsI GAAGAC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 768
BccI CCATC 3 cut(s) 260, 292, 826
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BclI TGATCA 4 cut(s) 54, 166, 193, 199
BcoDI GTCTC 2 cut(s) 232, 833
BfaI CTAG 5 cut(s) 182, 222, 236, 809, 887
BfmI CTRYAG 3 cut(s) 546, 849, 879
BfoI RGCGCY 1 cut(s) 788
BlpI GCTNAGC 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 4 cut(s) 207, 646, 746, 1012
BmsI GCATC 1 cut(s) 344
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 1 cut(s) 768
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 886
BsaBI GATNNNNATC 2 cut(s) 165, 198
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 3 cut(s) 303, 588, 823
Bsc4I CCNNNNNNNGG 3 cut(s) 578, 719, 740
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 636
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 2 cut(s) 165, 198
BseDI CCNNGG 3 cut(s) 303, 588, 823
BseGI GGATG 5 cut(s) 154, 275, 359, 584, 730
BseJI GATNNNNATC 2 cut(s) 165, 198
BseLI CCNNNNNNNGG 3 cut(s) 578, 719, 740
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 3 cut(s) 56, 782, 1040
BseNI ACTGG 1 cut(s) 636
BseSI GKGCMC 1 cut(s) 747
BsgI GTGCAG 5 cut(s) 105, 322, 435, 594, 1018
BshFI GGCC 2 cut(s) 302, 593
BshNI GGYRCC 2 cut(s) 644, 744
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 3 cut(s) 578, 719, 740
BsmAI GTCTC 2 cut(s) 232, 833
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 2 cut(s) 302, 593
Bso31I GGTCTC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
Bsp1720I GCTNAGC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 3 cut(s) 572, 734, 797
BspANI GGCC 2 cut(s) 302, 593
BspCNI CTCAG 3 cut(s) 55, 781, 1039
BspHI TCATGA 2 cut(s) 163, 196
BspLI GGNNCC 4 cut(s) 207, 646, 746, 1012
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 2 cut(s) 98, 129
BspT107I GGYRCC 2 cut(s) 644, 744
BspTNI GGTCTC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 1 cut(s) 636
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 3 cut(s) 303, 588, 823
BssMI GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
BssT1I CCWWGG 2 cut(s) 303, 588
Bst4CI ACNGT 5 cut(s) 290, 692, 728, 827, 977
Bst6I CTCTTC 3 cut(s) 444, 565, 570
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 42, 768, 1026
BstDSI CCRYGG 2 cut(s) 588, 823
BstF5I GGATG 5 cut(s) 154, 275, 359, 584, 730
BstH2I RGCGCY 1 cut(s) 788
BstHHI GCGC 3 cut(s) 51, 787, 998
BstKTI GATC 9 cut(s) 57, 106, 137, 163, 169, 196, 202, 245, 817
BstMAI GTCTC 2 cut(s) 232, 833
BstMBI GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
BstMWI GCNNNNNNNGC 4 cut(s) 43, 485, 742, 890
BstSFI CTRYAG 3 cut(s) 546, 849, 879
BstSLI GKGCMC 1 cut(s) 747
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 2 cut(s) 302, 593
BtgI CCRYGG 2 cut(s) 588, 823
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 5 cut(s) 154, 275, 359, 584, 730
BtsIMutI CAGTG 1 cut(s) 629
Cac8I GCNNGC 1 cut(s) 39
CciI TCATGA 2 cut(s) 163, 196
CfoI GCGC 3 cut(s) 51, 787, 998
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 3 cut(s) 233, 374, 951
CviQI GTAC 3 cut(s) 233, 374, 951
DdeI CTNAG 3 cut(s) 42, 768, 1026
DpnI GATC 9 cut(s) 56, 105, 136, 162, 168, 195, 201, 244, 816
DpnII GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
EaeI YGGCCR 2 cut(s) 300, 591
Eam1104I CTCTTC 3 cut(s) 444, 565, 570
EarI CTCTTC 3 cut(s) 444, 565, 570
Eco130I CCWWGG 2 cut(s) 303, 588
Eco31I GGTCTC 1 cut(s) 232
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 7
Eco47III AGCGCT 1 cut(s) 786
Eco57I CTGAAG 1 cut(s) 551
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 2 cut(s) 303, 588
ErhI CCWWGG 2 cut(s) 303, 588
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 1 cut(s) 303
FbaI TGATCA 4 cut(s) 54, 166, 193, 199
FokI GGATG 5 cut(s) 141, 282, 366, 571, 717
FspBI CTAG 5 cut(s) 182, 222, 236, 809, 887
FspI TGCGCA 1 cut(s) 50
GlaI GCGC 3 cut(s) 50, 786, 997
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 788
HaeIII GGCC 2 cut(s) 302, 593
HapII CCGG 1 cut(s) 642
HhaI GCGC 3 cut(s) 51, 787, 998
Hin6I GCGC 3 cut(s) 49, 785, 996
HinP1I GCGC 3 cut(s) 49, 785, 996
HpaII CCGG 1 cut(s) 642
HphI GGTGA 1 cut(s) 7
Hpy166II GTNNAC 1 cut(s) 876
Hpy188I TCNGA 6 cut(s) 59, 108, 247, 366, 405, 865
Hpy188III TCNNGA 5 cut(s) 101, 158, 164, 170, 197
Hpy8I GTNNAC 1 cut(s) 876
HpyAV CCTTC 3 cut(s) 127, 210, 413
HpyCH4III ACNGT 5 cut(s) 290, 692, 728, 827, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 485, 742, 890
HpyF3I CTNAG 3 cut(s) 42, 768, 1026
HspAI GCGC 3 cut(s) 49, 785, 996
Ksp22I TGATCA 4 cut(s) 54, 166, 193, 199
Kzo9I GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
LmnI GCTCC 2 cut(s) 256, 1016
LweI GCATC 1 cut(s) 344
MaeI CTAG 5 cut(s) 182, 222, 236, 809, 887
MaeIII GTNAC 2 cut(s) 187, 722
MalI GATC 9 cut(s) 56, 105, 136, 162, 168, 195, 201, 244, 816
MbiI CCGCTC 1 cut(s) 574
MboI GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
MboII GAAGA 4 cut(s) 101, 431, 552, 557
MhlI GDGCHC 1 cut(s) 747
MlsI TGGCCA 2 cut(s) 302, 593
MluCI AATT 1 cut(s) 760
MluNI TGGCCA 2 cut(s) 302, 593
MnlI CCTC 9 cut(s) 273, 382, 447, 453, 616, 777, 908, 982, 1035
Mox20I TGGCCA 2 cut(s) 302, 593
MroXI GAANNNNTTC 1 cut(s) 177
MscI TGGCCA 2 cut(s) 302, 593
MseI TTAA 3 cut(s) 212, 930, 972
MslI CAYNNNNRTG 1 cut(s) 777
Msp20I TGGCCA 2 cut(s) 302, 593
MspA1I CMGCKG 4 cut(s) 46, 73, 449, 797
MspI CCGG 1 cut(s) 642
MwoI GCNNNNNNNGC 4 cut(s) 43, 485, 742, 890
NcoI CCATGG 1 cut(s) 588
NdeII GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
NlaIV GGNNCC 4 cut(s) 207, 646, 746, 1012
NsbI TGCGCA 1 cut(s) 50
PagI TCATGA 2 cut(s) 163, 196
PdmI GAANNNNTTC 1 cut(s) 177
PshBI ATTAAT 1 cut(s) 930
PspN4I GGNNCC 4 cut(s) 207, 646, 746, 1012
PspPI GGNCC 1 cut(s) 7
PstI CTGCAG 1 cut(s) 853
PvuII CAGCTG 3 cut(s) 46, 73, 449
RsaI GTAC 3 cut(s) 234, 375, 952
RsaNI GTAC 3 cut(s) 233, 374, 951
RseI CAYNNNNRTG 1 cut(s) 777
SaqAI TTAA 3 cut(s) 212, 930, 972
Sau3AI GATC 9 cut(s) 54, 103, 134, 160, 166, 193, 199, 242, 814
Sau96I GGNCC 1 cut(s) 7
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 3 cut(s) 546, 849, 879
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 1 cut(s) 901
SmoI CTYRAG 1 cut(s) 901
Sse9I AATT 1 cut(s) 760
SsiI CCGC 3 cut(s) 572, 734, 797
SspI AATATT 1 cut(s) 928
SspMI CTAG 5 cut(s) 182, 222, 236, 809, 887
StyI CCWWGG 2 cut(s) 303, 588
TaaI ACNGT 5 cut(s) 290, 692, 728, 827, 977
TaqI TCGA 2 cut(s) 817, 830
TaqII GACCGA 1 cut(s) 147
TasI AATT 1 cut(s) 760
TauI GCSGC 2 cut(s) 574, 800
Tru1I TTAA 3 cut(s) 212, 930, 972
Tru9I TTAA 3 cut(s) 212, 930, 972
TscAI CASTG 1 cut(s) 636
TspDTI ATGAA 4 cut(s) 432, 573, 673, 926
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 930
XapI RAATTY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 2 cut(s) 628, 682
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 5 cut(s) 182, 222, 236, 809, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.