Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002724

Physical Location & Seq

Genomic Coordinates Reverse (-)

8267211 .. 8270166

Gene Structure 2956 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1111200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGCCAAAGGAAGTGGACTCTCCATTGATTCAGATCCATTTTCCTTCTCTCTCCACCACCCTATAGTCCTCAACTCCTTCCAGGAAGACCAATATAACCACCACCTCCCACCACTCTTCACCACCAAAAAACAACTTCACCACCACCACTCATCACTCATGCTCATGGACAACTCGACCGCTATTCACCGCCGATCTCCTCCGCCCCCTCCTCCCACGACTATCCGGTTCGCAGTCAACCTCAACCGCTCCCACGACGACGATGTTCAGCACTCTCCTGACTCACCACCACCCTCCAACGAGCAACGCGAGGTCATCGACGAGCGGGACTTCTTTGCCGATAGCAAGAACCACCTCGATCACGAAAAGTCCGTCGGCTCCGACGACCCTGCCAATAAAATGGACTTACATGGCCCCACAGACATGGAATTCAACGTAAATACTGGTTTGAATCTTCTGCTTACAAACACTAGTACTGACCATTCAGTGGCGGAAGATGGTATTCCATCAAACATAGAGGATAAAAGAGCTAAAAGTGAGCTGGCTGTTCTTCAAGCTGAGCTCGAGCGGTTGAACGTGGAGAATCAACGGTTGAGGGGGATGCTTAATCAGGTGACCACCAATTACAATGCTCTTCAGGTGCATTTGCTGAATTTGACGCAAAGCCAGAAGGCTGATCAGAACAGTAGCGGTGCCGAAGGCCATCGAGTGGCGGTGGAAGAAAAGAAGCTTATGAATGGCAATGGATCATCACTAGTGGTGCCGAGGCAGTTCATCGATCTTGGGTTAGCTGCTAAAAATGGTGACGCTGATGAGCATTCACAGTCTTCGTCTGATGAACGAAGTCGTGGCGAACGGTCTGGATCGCTTGGAGATAATATGAAGGCTGCAGGGCACAGTGATGACCAAGGGATCGGTTTTGATCACGAAAAGAAGGATTTCGGGAGACGAATTGGGAGAGATGAGAGCCCAGATCAGCCGTCACAGAGTTGGAGGCCTAACAAAGTTCCGAGGCTCAGTTCTCCGAAAGAGGTTGATCAAACCGAGGCTACAATGAGGAAGGCTCGGGTCTCGGTTAGAGCTCGATCCGAGGCGCCTATGATCACTGATGGATGTCAATGGCGAAAGTATGGGCAAAAGATGGCAAAAGGAAACCCTTGTCCTCGTGCTTATTATCGATGCACCATGGCTGCTGGTTGCCCGGTTCGAAAACAAGTACAAAGATGTGCAGAAGATAGGACAATCCTAATCACTACATATGAAGGCAATCACAACCACCCATTGCCTCCGGCAGCAATGGCAATGGCATCGACTACATCATCCGCGGCGCGGATGTTGCTCTCGGGGGCTATGCCGAGTGCCGACAGCCTAATAGACTCAAACTTCCTCAGCAGGACAATCCTCCCATACTCCTCTAGCATGGCCACCATCTCCGCCTCAGCGCCATTCCCTACCGTTACATTGGACTTAACACAGTCACCGAACCCTTTACAACAATTCCAAAGGCCACCAGGCCAATTCAACATTCCATTCCCAAACCCTAATCAGAATTTCACCAGTGGCCCTGCCTCATTGCTGCCTCAAATTTTTAGTCAGGCGCTATACAACCAGTCTAAATTCTCTGGCCTCCACATGTCACAGGATACAGACCGTGCGGCCGTCCAATTAGGTCACCAATCGCAACCCGGGCAGCAGCAGGGCTCACTGGCCGATCTTACTGCCGCCACCGCTGCCATTGCAGCTGATCCAAACTTCACTGCAGCCCTAGCAGCTGCCATCACCTCTATCATTGGCAATGCTCATCCAAACAACAATGTTGCCAACCCAGCAACCAACTCAAACAACAACAATGCCAATGCCACCAAGCAACAGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

626

Amino Acids

68.2

Weight (kDa)

6.33

Isoelectric Point (pI)

49.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	370 - 427	1.2e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01720
fragaria_vesca	FvH4_1g26200 FvH4_1g26200 FvH4_6g42870 FvH4_6g42870 FvH4_6g42870
malus_domestica	MD09G1111200.v1.1 MD17G1099000.v1.1
prunus_persica	Prupe.3G002300_v2.0.a1 Prupe.3G214800_v2.0.a1
pyrus_communis	pycom09g03370
rosa_chinensis	RchiOBHm_Chr2g0158801 RchiOBHm_Chr3g0485691 RchiOBHm_Chr3g0485711
rosa_laevigata	RLG00000021114 RLG00000023129 RLG00000023130
rosa_multiflora	Rmu_sc0003005.1_g000017 Rmu_sc0003005.1_g000026 Rmu_ssc0000129.1_g000002
rosa_roxburghii	Rroxscaffold_6G00396400 Rroxscaffold_6G00396500 Rroxscaffold_6G00396810 Rroxscaffold_6G00396830
rosa_rugosa	Rorug02G0472600 Rorug03G0220800
rosa_samantha	Rh2AG538400 Rh2BG551100 Rh2CG521600 Rh2DG560900 Rh3AG270000 Rh3AG270200 Rh3BG305800 Rh3CG303900 Rh3CG304200 Rh3DG300200
rosa_wichuraiana	Rw0G006930 Rw0G009910 Rw2G044560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	692, 760, 1093
AccB7I	CCANNNNNTGG	2 cut(s)	487, 709
AccBSI	CCGCTC	3 cut(s)	249, 325, 568
AccII	CGCG	3 cut(s)	309, 1325, 1330
AclWI	GGATC	6 cut(s)	29, 754, 871, 920, 1080, 1739
AcoI	YGGCCR	4 cut(s)	3, 1422, 1656, 1707
AcsI	RAATTY	5 cut(s)	428, 652, 1549, 1584, 1616
AcuI	CTGAAG	1 cut(s)	620
AcyI	GRCGYC	1 cut(s)	1094
AfaI	GTAC	2 cut(s)	475, 1218
AfiI	CCNNNNNNNGG	3 cut(s)	487, 709, 1329
AflIII	ACRYGT	1 cut(s)	1632
AgsI	TTSAA	5 cut(s)	433, 451, 554, 574, 1522
AhlI	ACTAGT	2 cut(s)	470, 754
AjnI	CCWGG	2 cut(s)	81, 1510
AjuI	GAANNNNNNNTTGG	2 cut(s)	1509, 1541
AluBI	AGCT	9 cut(s)	530, 541, 557, 562, 730, 791, 1082, 1742, 1772
AluI	AGCT	9 cut(s)	530, 541, 557, 562, 730, 791, 1082, 1742, 1772
Alw21I	GWGCWC	2 cut(s)	564, 1084
Alw26I	GTCTC	2 cut(s)	940, 1075
AlwI	GGATC	6 cut(s)	29, 754, 871, 920, 1080, 1739
Ama87I	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
ApoI	RAATTY	5 cut(s)	428, 652, 1549, 1584, 1616
ArsI	GACNNNNNNTTYG	4 cut(s)	357, 389, 1576, 1608
Asp700I	GAANNNNTTC	1 cut(s)	938
AspLEI	GCGC	4 cut(s)	1096, 1330, 1444, 1600
AspS9I	GGNCC	2 cut(s)	413, 1562
AsuC2I	CCSGG	3 cut(s)	1202, 1686, 1687
AsuII	TTCGAA	1 cut(s)	1207
AvaI	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
BaeGI	GKGCMC	1 cut(s)	897
BalI	TGGCCA	2 cut(s)	5, 1424
BanI	GGYRCC	3 cut(s)	692, 760, 1093
BanII	GRGCYC	4 cut(s)	564, 971, 1084, 1703
BauI	CACGAG	1 cut(s)	1164
BbsI	GAAGAC	2 cut(s)	93, 819
Bbv12I	GWGCWC	2 cut(s)	564, 1084
BbvCI	CCTCAGC	2 cut(s)	1388, 1438
BccI	CCATC	7 cut(s)	491, 514, 711, 1103, 1135, 1436, 1784
BceAI	ACGGC	2 cut(s)	964, 1643
BcgI	CGANNNNNNTGC	6 cut(s)	371, 405, 1290, 1324, 1700, 1734
BciT130I	CCWGG	2 cut(s)	83, 1512
BciVI	GTATCC	1 cut(s)	1636
BclI	TGATCA	4 cut(s)	676, 922, 1036, 1101
BcnI	CCSGG	3 cut(s)	1202, 1686, 1687
BcoDI	GTCTC	2 cut(s)	940, 1075
BcuI	ACTAGT	2 cut(s)	470, 754
BfaI	CTAG	4 cut(s)	471, 755, 1416, 1766
BfmI	CTRYAG	3 cut(s)	63, 888, 1758
BfoI	RGCGCY	3 cut(s)	1097, 1445, 1601
BfuI	GTATCC	1 cut(s)	1636
BlpI	GCTNAGC	1 cut(s)	558
BmcAI	AGTACT	1 cut(s)	475
Bme1390I	CCNGG	5 cut(s)	83, 1202, 1512, 1686, 1687
BmeT110I	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
BmgT120I	GGNCC	2 cut(s)	413, 1562
BmiI	GGNNCC	5 cut(s)	379, 415, 694, 762, 1095
BmrFI	CCNGG	5 cut(s)	83, 1202, 1512, 1686, 1687
BmsI	GCATC	3 cut(s)	591, 1169, 1316
BpiI	GAAGAC	2 cut(s)	93, 819
BplI	GAGNNNNNCTC	2 cut(s)	1048, 1080
Bpu10I	CCTNAGC	2 cut(s)	1388, 1438
Bpu1102I	GCTNAGC	1 cut(s)	558
Bpu14I	TTCGAA	1 cut(s)	1207
BpuMI	CCSGG	3 cut(s)	1202, 1686, 1687
Bsa29I	ATCGAT	2 cut(s)	777, 1177
BsaBI	GATNNNNATC	1 cut(s)	33
BsaHI	GRCGYC	1 cut(s)	1094
BsaI	GGTCTC	1 cut(s)	1075
BsaJI	CCNNGG	8 cut(s)	764, 907, 1010, 1044, 1089, 1185, 1323, 1685
BsaWI	WCCGGW	1 cut(s)	225
Bsc4I	CCNNNNNNNGG	3 cut(s)	487, 709, 1329
Bse1I	ACTGG	4 cut(s)	448, 1557, 1609, 1710
Bse3DI	GCAATG	7 cut(s)	748, 1280, 1302, 1308, 1571, 1734, 1801
Bse8I	GATNNNNATC	1 cut(s)	33
BseBI	CCWGG	2 cut(s)	83, 1512
BseCI	ATCGAT	2 cut(s)	777, 1177
BseDI	CCNNGG	8 cut(s)	764, 907, 1010, 1044, 1089, 1185, 1323, 1685
BseGI	GGATG	5 cut(s)	606, 1118, 1319, 1338, 1801
BseJI	GATNNNNATC	1 cut(s)	33
BseLI	CCNNNNNNNGG	3 cut(s)	487, 709, 1329
BseMI	GCAATG	7 cut(s)	748, 1280, 1302, 1308, 1571, 1734, 1801
BseMII	CTCAG	4 cut(s)	549, 1030, 1402, 1452
BseNI	ACTGG	4 cut(s)	448, 1557, 1609, 1710
BseRI	GAGGAG	3 cut(s)	189, 201, 1402
BseSI	GKGCMC	1 cut(s)	897
BseX3I	CGGCCG	1 cut(s)	1656
BseYI	CCCAGC	1 cut(s)	1825
BsgI	GTGCAG	1 cut(s)	1248
Bsh1236I	CGCG	3 cut(s)	309, 1325, 1330
Bsh1285I	CGRYCG	2 cut(s)	180, 1659
BshNI	GGYRCC	3 cut(s)	692, 760, 1093
BshVI	ATCGAT	2 cut(s)	777, 1177
BsiEI	CGRYCG	2 cut(s)	180, 1659
BsiHKAI	GWGCWC	2 cut(s)	564, 1084
BsiHKCI	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
BsiSI	CCGG	4 cut(s)	226, 1202, 1289, 1686
BslFI	GGGAC	1 cut(s)	341
BslI	CCNNNNNNNGG	3 cut(s)	487, 709, 1329
BsmAI	GTCTC	2 cut(s)	940, 1075
BsmBI	CGTCTC	1 cut(s)	940
BsmFI	GGGAC	1 cut(s)	341
BsmI	GAATGC	1 cut(s)	817
Bso31I	GGTCTC	1 cut(s)	1075
BsoBI	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
Bsp119I	TTCGAA	1 cut(s)	1207
Bsp1286I	GDGCHC	5 cut(s)	564, 897, 971, 1084, 1703
Bsp1720I	GCTNAGC	1 cut(s)	558
Bsp19I	CCATGG	1 cut(s)	1185
BspCNI	CTCAG	4 cut(s)	550, 1029, 1401, 1451
BspDI	ATCGAT	2 cut(s)	777, 1177
BspFNI	CGCG	3 cut(s)	309, 1325, 1330
BspLI	GGNNCC	5 cut(s)	379, 415, 694, 762, 1095
BspMAI	CTGCAG	2 cut(s)	892, 1762
BspPI	GGATC	6 cut(s)	29, 754, 871, 920, 1080, 1739
BspQI	GCTCTTC	1 cut(s)	639
BspT104I	TTCGAA	1 cut(s)	1207
BspT107I	GGYRCC	3 cut(s)	692, 760, 1093
BspTNI	GGTCTC	1 cut(s)	1075
BsrBI	CCGCTC	3 cut(s)	249, 325, 568
BsrDI	GCAATG	7 cut(s)	748, 1280, 1302, 1308, 1571, 1734, 1801
BsrI	ACTGG	4 cut(s)	448, 1557, 1609, 1710
BssECI	CCNNGG	8 cut(s)	764, 907, 1010, 1044, 1089, 1185, 1323, 1685
BssNI	GRCGYC	1 cut(s)	1094
BssSI	CACGAG	1 cut(s)	1164
BssT1I	CCWWGG	2 cut(s)	907, 1185
Bst2BI	CACGAG	1 cut(s)	1164
Bst2UI	CCWGG	2 cut(s)	83, 1512
Bst4CI	ACNGT	8 cut(s)	591, 686, 825, 858, 899, 1456, 1476, 1652
Bst6I	CTCTTC	2 cut(s)	122, 639
BstACI	GRCGYC	1 cut(s)	1094
BstBI	TTCGAA	1 cut(s)	1207
BstC8I	GCNNGC	1 cut(s)	543
BstDEI	CTNAG	4 cut(s)	558, 1016, 1388, 1438
BstDSI	CCRYGG	2 cut(s)	1185, 1323
BstEII	GGTNACC	2 cut(s)	613, 1670
BstF5I	GGATG	5 cut(s)	606, 1118, 1319, 1338, 1801
BstFNI	CGCG	3 cut(s)	309, 1325, 1330
BstH2I	RGCGCY	3 cut(s)	1097, 1445, 1601
BstHHI	GCGC	4 cut(s)	1096, 1330, 1444, 1600
BstMAI	GTCTC	2 cut(s)	940, 1075
BstMCI	CGRYCG	2 cut(s)	180, 1659
BstNI	CCWGG	2 cut(s)	83, 1512
BstNSI	RCATGY	1 cut(s)	1636
BstPI	GGTNACC	2 cut(s)	613, 1670
BstSCI	CCNGG	5 cut(s)	81, 1200, 1510, 1684, 1685
BstSFI	CTRYAG	3 cut(s)	63, 888, 1758
BstSLI	GKGCMC	1 cut(s)	897
BstUI	CGCG	3 cut(s)	309, 1325, 1330
BstV2I	GAAGAC	2 cut(s)	93, 819
BstX2I	RGATCY	1 cut(s)	34
BstXI	CCANNNNNNTGG	2 cut(s)	400, 424
BstYI	RGATCY	1 cut(s)	34
BstZI	CGGCCG	1 cut(s)	1656
Bsu15I	ATCGAT	2 cut(s)	777, 1177
BsuI	GTATCC	1 cut(s)	1636
BsuTUI	ATCGAT	2 cut(s)	777, 1177
BtgI	CCRYGG	2 cut(s)	1185, 1323
BtsCI	GGATG	5 cut(s)	606, 1118, 1319, 1338, 1801
BtsI	GCAGTG	1 cut(s)	1755
BtsIMutI	CAGTG	6 cut(s)	492, 904, 1104, 1564, 1703, 1755
Cac8I	GCNNGC	1 cut(s)	543
CfoI	GCGC	4 cut(s)	1096, 1330, 1444, 1600
Cfr13I	GGNCC	2 cut(s)	413, 1562
Cfr42I	CCGCGG	1 cut(s)	1326
Cfr9I	CCCGGG	1 cut(s)	1685
ClaI	ATCGAT	2 cut(s)	777, 1177
CseI	GACGC	2 cut(s)	667, 815
Csp6I	GTAC	2 cut(s)	474, 1217
CspCI	CAANNNNNGTGG	1 cut(s)	30
CviAII	CATG	7 cut(s)	160, 166, 410, 424, 1186, 1420, 1633
CviQI	GTAC	2 cut(s)	474, 1217
DdeI	CTNAG	4 cut(s)	558, 1016, 1388, 1438
DinI	GGCGCC	1 cut(s)	1095
EaeI	YGGCCR	4 cut(s)	3, 1422, 1656, 1707
EagI	CGGCCG	1 cut(s)	1656
Eam1104I	CTCTTC	2 cut(s)	122, 639
EarI	CTCTTC	2 cut(s)	122, 639
EciI	GGCGGA	3 cut(s)	192, 506, 1423
Ecl136II	GAGCTC	2 cut(s)	562, 1082
EclXI	CGGCCG	1 cut(s)	1656
Eco130I	CCWWGG	2 cut(s)	907, 1185
Eco147I	AGGCCT	1 cut(s)	997
Eco24I	GRGCYC	4 cut(s)	564, 971, 1084, 1703
Eco31I	GGTCTC	1 cut(s)	1075
Eco52I	CGGCCG	1 cut(s)	1656
Eco53kI	GAGCTC	2 cut(s)	562, 1082
Eco57I	CTGAAG	1 cut(s)	620
Eco88I	CYCGRG	4 cut(s)	563, 1065, 1342, 1685
Eco91I	GGTNACC	2 cut(s)	613, 1670
EcoICRI	GAGCTC	2 cut(s)	562, 1082
EcoO65I	GGTNACC	2 cut(s)	613, 1670
EcoRI	GAATTC	1 cut(s)	428
EcoRII	CCWGG	2 cut(s)	81, 1510
EcoT14I	CCWWGG	2 cut(s)	907, 1185
EcoT38I	GRGCYC	4 cut(s)	564, 971, 1084, 1703
EgeI	GGCGCC	1 cut(s)	1095
EheI	GGCGCC	1 cut(s)	1095
ErhI	CCWWGG	2 cut(s)	907, 1185
Esp3I	CGTCTC	1 cut(s)	940
FaeI	CATG	7 cut(s)	163, 169, 413, 427, 1189, 1423, 1636
FaqI	GGGAC	1 cut(s)	341
FatI	CATG	7 cut(s)	159, 165, 409, 423, 1185, 1419, 1632
FauI	CCCGC	1 cut(s)	318
FauNDI	CATATG	1 cut(s)	1258
FbaI	TGATCA	4 cut(s)	676, 922, 1036, 1101
FokI	GGATG	5 cut(s)	613, 1125, 1306, 1345, 1788
FriOI	GRGCYC	4 cut(s)	564, 971, 1084, 1703
FspBI	CTAG	4 cut(s)	471, 755, 1416, 1766
GlaI	GCGC	4 cut(s)	1095, 1329, 1443, 1599
GsaI	CCCAGC	1 cut(s)	1829
HaeII	RGCGCY	3 cut(s)	1097, 1445, 1601
HapII	CCGG	4 cut(s)	226, 1202, 1289, 1686
HgaI	GACGC	2 cut(s)	667, 815
HhaI	GCGC	4 cut(s)	1096, 1330, 1444, 1600
Hin1I	GRCGYC	1 cut(s)	1094
Hin1II	CATG	7 cut(s)	163, 169, 413, 427, 1189, 1423, 1636
Hin6I	GCGC	4 cut(s)	1094, 1328, 1442, 1598
HinP1I	GCGC	4 cut(s)	1094, 1328, 1442, 1598
HincII	GTYRAC	1 cut(s)	238
HindII	GTYRAC	1 cut(s)	238
HindIII	AAGCTT	1 cut(s)	728
HinfI	GANTC	6 cut(s)	18, 29, 281, 451, 583, 1376
HpaII	CCGG	4 cut(s)	226, 1202, 1289, 1686
Hpy166II	GTNNAC	2 cut(s)	17, 238
Hpy188I	TCNGA	8 cut(s)	34, 382, 681, 835, 1011, 1026, 1090, 1548
Hpy188III	TCNNGA	5 cut(s)	278, 362, 861, 926, 943
Hpy8I	GTNNAC	2 cut(s)	17, 238
Hpy99I	CGWCG	5 cut(s)	260, 263, 323, 377, 386
HpyAV	CCTTC	8 cut(s)	55, 88, 664, 692, 877, 928, 1054, 1256
HpyCH4III	ACNGT	8 cut(s)	591, 686, 825, 858, 899, 1456, 1476, 1652
HpyCH4IV	ACGT	2 cut(s)	435, 576
HpyCH4V	TGCA	6 cut(s)	643, 890, 1182, 1229, 1739, 1760
HpyF3I	CTNAG	4 cut(s)	558, 1016, 1388, 1438
HpySE526I	ACGT	2 cut(s)	435, 576
Hsp92I	GRCGYC	1 cut(s)	1094
Hsp92II	CATG	7 cut(s)	163, 169, 413, 427, 1189, 1423, 1636
HspAI	GCGC	4 cut(s)	1094, 1328, 1442, 1598
KasI	GGCGCC	1 cut(s)	1093
Ksp22I	TGATCA	4 cut(s)	676, 922, 1036, 1101
KspI	CCGCGG	1 cut(s)	1326
LguI	GCTCTTC	1 cut(s)	639
LmnI	GCTCC	2 cut(s)	254, 383
LweI	GCATC	3 cut(s)	591, 1169, 1316
MaeI	CTAG	4 cut(s)	471, 755, 1416, 1766
MaeII	ACGT	2 cut(s)	435, 576
MaeIII	GTNAC	7 cut(s)	613, 803, 981, 1456, 1476, 1635, 1670
MbiI	CCGCTC	3 cut(s)	249, 325, 568
MboII	GAAGA	9 cut(s)	98, 109, 446, 506, 542, 626, 731, 819, 1244
MflI	RGATCY	1 cut(s)	34
MhlI	GDGCHC	5 cut(s)	564, 897, 971, 1084, 1703
MlsI	TGGCCA	2 cut(s)	5, 1424
MluNI	TGGCCA	2 cut(s)	5, 1424
Mly113I	GGCGCC	1 cut(s)	1094
MlyI	GAGTC	3 cut(s)	12, 275, 1370
MmeI	TCCRAC	3 cut(s)	321, 405, 971
Mox20I	TGGCCA	2 cut(s)	5, 1424
MroXI	GAANNNNTTC	1 cut(s)	938
MscI	TGGCCA	2 cut(s)	5, 1424
MseI	TTAA	2 cut(s)	606, 1469
MslI	CAYNNNNRTG	3 cut(s)	164, 422, 900
Msp20I	TGGCCA	2 cut(s)	5, 1424
MspA1I	CMGCKG	4 cut(s)	1325, 1730, 1742, 1772
MspI	CCGG	4 cut(s)	226, 1202, 1289, 1686
MspR9I	CCNGG	5 cut(s)	83, 1202, 1512, 1686, 1687
Mva1269I	GAATGC	1 cut(s)	817
MvaI	CCWGG	2 cut(s)	83, 1512
MvnI	CGCG	3 cut(s)	309, 1325, 1330
NarI	GGCGCC	1 cut(s)	1094
NciI	CCSGG	3 cut(s)	1202, 1686, 1687
NcoI	CCATGG	1 cut(s)	1185
NdeI	CATATG	1 cut(s)	1258
NlaIII	CATG	7 cut(s)	163, 169, 413, 427, 1189, 1423, 1636
NlaIV	GGNNCC	5 cut(s)	379, 415, 694, 762, 1095
NmeAIII	GCCGAG	2 cut(s)	789, 1380
NmuCI	GTSAC	6 cut(s)	613, 803, 981, 1476, 1635, 1670
NspI	RCATGY	1 cut(s)	1636
NspV	TTCGAA	1 cut(s)	1207
PaeR7I	CTCGAG	1 cut(s)	563
PceI	AGGCCT	1 cut(s)	997
PciI	ACATGT	1 cut(s)	1632
PciSI	GCTCTTC	1 cut(s)	639
PcsI	WCGNNNNNNNCGW	3 cut(s)	306, 369, 381
PctI	GAATGC	1 cut(s)	817
PdmI	GAANNNNTTC	1 cut(s)	938
PfeI	GAWTC	3 cut(s)	29, 451, 583
PflMI	CCANNNNNTGG	2 cut(s)	487, 709
PfoI	TCCNGGA	1 cut(s)	81
PleI	GAGTC	3 cut(s)	12, 275, 1370
PluTI	GGCGCC	1 cut(s)	1097
PpsI	GAGTC	3 cut(s)	12, 275, 1370
PscI	ACATGT	1 cut(s)	1632
Psp124BI	GAGCTC	2 cut(s)	564, 1084
Psp6I	CCWGG	2 cut(s)	81, 1510
PspEI	GGTNACC	2 cut(s)	613, 1670
PspFI	CCCAGC	1 cut(s)	1825
PspGI	CCWGG	2 cut(s)	81, 1510
PspN4I	GGNNCC	5 cut(s)	379, 415, 694, 762, 1095
PspPI	GGNCC	2 cut(s)	413, 1562
PspXI	VCTCGAGB	1 cut(s)	563
PstI	CTGCAG	2 cut(s)	892, 1762
PsuI	RGATCY	1 cut(s)	34
PvuII	CAGCTG	2 cut(s)	1742, 1772
RsaI	GTAC	2 cut(s)	475, 1218
RsaNI	GTAC	2 cut(s)	474, 1217
RseI	CAYNNNNRTG	3 cut(s)	164, 422, 900
SacI	GAGCTC	2 cut(s)	564, 1084
SacII	CCGCGG	1 cut(s)	1326
SapI	GCTCTTC	1 cut(s)	639
SaqAI	TTAA	2 cut(s)	606, 1469
Sau96I	GGNCC	2 cut(s)	413, 1562
ScaI	AGTACT	1 cut(s)	475
SchI	GAGTC	3 cut(s)	12, 275, 1370
ScrFI	CCNGG	5 cut(s)	83, 1202, 1512, 1686, 1687
SduI	GDGCHC	5 cut(s)	564, 897, 971, 1084, 1703
SfaNI	GCATC	3 cut(s)	591, 1169, 1316
SfcI	CTRYAG	3 cut(s)	63, 888, 1758
SfoI	GGCGCC	1 cut(s)	1095
Sfr274I	CTCGAG	1 cut(s)	563
Sfr303I	CCGCGG	1 cut(s)	1326
SfuI	TTCGAA	1 cut(s)	1207
SgrBI	CCGCGG	1 cut(s)	1326
SlaI	CTCGAG	1 cut(s)	563
SmaI	CCCGGG	1 cut(s)	1687
SmiMI	CAYNNNNRTG	3 cut(s)	164, 422, 900
SmlI	CTYRAG	1 cut(s)	563
SmoI	CTYRAG	1 cut(s)	563
SpeI	ACTAGT	2 cut(s)	470, 754
SseBI	AGGCCT	1 cut(s)	997
SspDI	GGCGCC	1 cut(s)	1093
SspMI	CTAG	4 cut(s)	471, 755, 1416, 1766
SstI	GAGCTC	2 cut(s)	564, 1084
StuI	AGGCCT	1 cut(s)	997
StyD4I	CCNGG	5 cut(s)	81, 1200, 1510, 1684, 1685
StyI	CCWWGG	2 cut(s)	907, 1185
TaaI	ACNGT	8 cut(s)	591, 686, 825, 858, 899, 1456, 1476, 1652
TaiI	ACGT	2 cut(s)	438, 579
TatI	WGTACW	2 cut(s)	473, 1216
TauI	GCSGC	3 cut(s)	1328, 1658, 1724
TfiI	GAWTC	3 cut(s)	29, 451, 583
Tru1I	TTAA	2 cut(s)	606, 1469
Tru9I	TTAA	2 cut(s)	606, 1469
TscAI	CASTG	6 cut(s)	492, 904, 1111, 1564, 1710, 1762
TseFI	GTSAC	6 cut(s)	613, 803, 981, 1476, 1635, 1670
Tsp45I	GTSAC	6 cut(s)	613, 803, 981, 1476, 1635, 1670
TspDTI	ATGAA	5 cut(s)	749, 763, 852, 896, 1275
TspGWI	ACGGA	1 cut(s)	361
TspMI	CCCGGG	1 cut(s)	1685
TspRI	CASTG	6 cut(s)	492, 904, 1111, 1564, 1710, 1762
Van91I	CCANNNNNTGG	2 cut(s)	487, 709
XapI	RAATTY	5 cut(s)	428, 652, 1549, 1584, 1616
XceI	RCATGY	1 cut(s)	1636
XhoI	CTCGAG	1 cut(s)	563
XmaI	CCCGGG	1 cut(s)	1685
XmnI	GAANNNNTTC	1 cut(s)	938
XspI	CTAG	4 cut(s)	471, 755, 1416, 1766
ZrmI	AGTACT	1 cut(s)	475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.