RLG00000023130
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002724
Physical Location & Seq
Reverse (-)
18648035 .. 18650699
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023130

Sequence Viewer

Length: 1293 bp
ATGGATGAACAACGCTGTGACTTGAAACACTTGCACTCGGACCAGAGCTTTCTGGGGCTCAGTAGTAGTTTCGATCATCGTCTAGTTGAACCCCCAATGAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCTTTTCCAATAATAGACTTCCCGATCAACCGGCGCCTGGTCATGCCGAGCTTCAAGTTGAGTTACAGCGAGAATACGAAGAGAATCAAAAGCTCAGAACCATGTTGGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCATGTGCGCATGATCAGACTTCAACAGCAGCTGCAAACTTGTGCAGAAGACAGCATTAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCAATCCCCCAAAAGTAGTTCTCATCACGATGATGATCAAGAACAAGTTCTTGCTGCTAGTGATCATGATCAGGTTCCTTCAAGAAGGGCCAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGTGACGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCCTGTCCCCGTGCTTACTATCGCTGCACGATGTTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGAATGGCAGAGGACAAGACCATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCTGCAGCTGCGATATCTATGGCTAAAACCACCTCAGCAGCAGCAGCCATGTTGATATCTGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAAAACACCCTACAGGGTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCGGAGCCCCTAACCCTAACCCTAATCCCATGCAGTTCATGCACAGTAGTGCTACATTTCCTATTCCTTTGCAGGGTTATAATCATCCGCTTATAAAGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGAACAACCCCACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCATCTCAAGTAGAATGAATAATGTTGAGAATATTAATGGTGGATTACAAGGAAGTACCAATGATATTAGTTCCCTTAACAGTAATGGGAATGAGGTTTGCGCTGCATTATCTGGCTCCCCACAGCTTCCTCAATCCTGTACCAGTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

431

Amino Acids

47.37

Weight (kDa)

6.39

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 170 - 227 1.8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 963, 977
Acc16I TGCGCA 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 817
AciI CCGC 3 cut(s) 815, 971, 1034
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 2 cut(s) 543, 834
AcsI RAATTY 1 cut(s) 997
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 3 cut(s) 477, 1189, 1273
AfeI AGCGCT 1 cut(s) 1023
AfiI CCNNNNNNNGG 3 cut(s) 176, 821, 956
AgsI TTSAA 9 cut(s) 25, 89, 118, 194, 305, 456, 750, 846, 997
AjnI CCWGG 2 cut(s) 175, 464
AleI CACNNNNGTG 1 cut(s) 930
AloI GAACNNNNNNTCC 2 cut(s) 588, 620
Alw26I GTCTC 2 cut(s) 475, 1070
AlwI GGATC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 1023
AoxI GGCC 3 cut(s) 462, 543, 834
ApoI RAATTY 1 cut(s) 997
AseI ATTAAT 2 cut(s) 339, 1167
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 4 cut(s) 175, 291, 1024, 1235
AspS9I GGNCC 3 cut(s) 40, 247, 462
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 2 cut(s) 40, 247
BalI TGGCCA 2 cut(s) 545, 836
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 2 cut(s) 60, 892
BbsI GAAGAC 1 cut(s) 336
BbvCI CCTCAGC 2 cut(s) 718, 1005
BccI CCATC 3 cut(s) 535, 1063, 1142
BcgI CGANNNNNNTGC 2 cut(s) 571, 605
BciT130I CCWGG 2 cut(s) 177, 466
BclI TGATCA 4 cut(s) 294, 409, 436, 442
BcoDI GTCTC 2 cut(s) 475, 1070
BfaI CTAG 4 cut(s) 83, 432, 1046, 1124
BfmI CTRYAG 4 cut(s) 687, 789, 1086, 1116
BfoI RGCGCY 2 cut(s) 176, 1025
Bme1390I CCNGG 2 cut(s) 177, 466
Bme18I GGWCC 2 cut(s) 40, 247
BmgT120I GGNCC 3 cut(s) 40, 247, 462
BmiI GGNNCC 4 cut(s) 174, 450, 889, 1249
BmrFI CCNGG 2 cut(s) 177, 466
BmsI GCATC 1 cut(s) 587
BoxI GACNNNNGTC 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 336
Bpu10I CCTNAGC 2 cut(s) 718, 1005
BpuEI CTTGAG 1 cut(s) 1123
BsaBI GATNNNNATC 2 cut(s) 408, 441
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 465, 546, 831, 1060
BsaWI WCCGGW 1 cut(s) 884
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 821, 956
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 879, 1275
Bse3DI GCAATG 1 cut(s) 1044
Bse8I GATNNNNATC 2 cut(s) 408, 441
BseBI CCWGG 2 cut(s) 177, 466
BseDI CCNNGG 4 cut(s) 465, 546, 831, 1060
BseGI GGATG 5 cut(s) 10, 518, 602, 827, 967
BseJI GATNNNNATC 2 cut(s) 408, 441
BseLI CCNNNNNNNGG 3 cut(s) 176, 821, 956
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 4 cut(s) 73, 247, 732, 1019
BseNI ACTGG 2 cut(s) 879, 1275
BsgI GTGCAG 3 cut(s) 345, 565, 837
BshFI GGCC 3 cut(s) 464, 545, 836
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 2 cut(s) 170, 885
BsiWI CGTACG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 3 cut(s) 176, 821, 956
BsmAI GTCTC 2 cut(s) 475, 1070
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 3 cut(s) 464, 545, 836
Bso31I GGTCTC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 60, 892
Bsp143I GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 3 cut(s) 815, 971, 1034
BspANI GGCC 3 cut(s) 464, 545, 836
BspCNI CTCAG 4 cut(s) 72, 246, 731, 1018
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 174, 450, 889, 1249
BspMAI CTGCAG 2 cut(s) 691, 1090
BspPI GGATC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 817
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 879, 1275
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 465, 546, 831, 1060
BssMI GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 546, 831
Bst2UI CCWGG 2 cut(s) 177, 466
Bst4CI ACNGT 4 cut(s) 533, 929, 1064, 1214
Bst6I CTCTTC 4 cut(s) 213, 687, 808, 813
BstACI GRCGYC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 922
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 4 cut(s) 59, 233, 718, 1005
BstDSI CCRYGG 2 cut(s) 831, 1060
BstF5I GGATG 5 cut(s) 10, 518, 602, 827, 967
BstH2I RGCGCY 2 cut(s) 176, 1025
BstHHI GCGC 4 cut(s) 175, 291, 1024, 1235
BstKTI GATC 9 cut(s) 76, 126, 166, 297, 349, 412, 439, 445, 488
BstMAI GTCTC 2 cut(s) 475, 1070
BstMBI GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
BstMWI GCNNNNNNNGC 4 cut(s) 728, 922, 979, 1127
BstNI CCWGG 2 cut(s) 177, 466
BstPAI GACNNNNGTC 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 175, 464
BstSFI CTRYAG 4 cut(s) 687, 789, 1086, 1116
BstV2I GAAGAC 1 cut(s) 336
BsuRI GGCC 3 cut(s) 464, 545, 836
BtgI CCRYGG 2 cut(s) 831, 1060
BtsCI GGATG 5 cut(s) 10, 518, 602, 827, 967
BtsIMutI CAGTG 1 cut(s) 872
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 313
CciI TCATGA 1 cut(s) 439
CfoI GCGC 4 cut(s) 175, 291, 1024, 1235
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 40, 247, 462
Csp6I GTAC 3 cut(s) 476, 1188, 1272
CviQI GTAC 3 cut(s) 476, 1188, 1272
DdeI CTNAG 4 cut(s) 59, 233, 718, 1005
DinI GGCGCC 1 cut(s) 174
DpnI GATC 9 cut(s) 75, 125, 165, 296, 348, 411, 438, 444, 487
DpnII GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
EaeI YGGCCR 2 cut(s) 543, 834
Eam1104I CTCTTC 4 cut(s) 213, 687, 808, 813
EarI CTCTTC 4 cut(s) 213, 687, 808, 813
Eco130I CCWWGG 2 cut(s) 546, 831
Eco24I GRGCYC 2 cut(s) 60, 892
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 2 cut(s) 699, 741
Eco47I GGWCC 2 cut(s) 40, 247
Eco47III AGCGCT 1 cut(s) 1023
Eco57I CTGAAG 1 cut(s) 794
EcoRII CCWGG 2 cut(s) 175, 464
EcoRV GATATC 2 cut(s) 699, 741
EcoT14I CCWWGG 2 cut(s) 546, 831
EcoT38I GRGCYC 2 cut(s) 60, 892
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 546, 831
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 546
FauNDI CATATG 1 cut(s) 658
FbaI TGATCA 4 cut(s) 294, 409, 436, 442
FokI GGATG 5 cut(s) 17, 525, 609, 814, 954
FriOI GRGCYC 2 cut(s) 60, 892
FspAI RTGCGCAY 1 cut(s) 290
FspBI CTAG 4 cut(s) 83, 432, 1046, 1124
FspI TGCGCA 1 cut(s) 290
GlaI GCGC 4 cut(s) 174, 290, 1023, 1234
HaeII RGCGCY 2 cut(s) 176, 1025
HaeIII GGCC 3 cut(s) 464, 545, 836
HapII CCGG 2 cut(s) 170, 885
HhaI GCGC 4 cut(s) 175, 291, 1024, 1235
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 4 cut(s) 173, 289, 1022, 1233
HinP1I GCGC 4 cut(s) 173, 289, 1022, 1233
HinfI GANTC 1 cut(s) 223
HpaII CCGG 2 cut(s) 170, 885
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 1113
Hpy188I TCNGA 8 cut(s) 40, 236, 299, 351, 490, 609, 648, 1102
Hpy188III TCNNGA 6 cut(s) 161, 344, 401, 413, 440, 456
Hpy8I GTNNAC 1 cut(s) 1113
HpyAV CCTTC 4 cut(s) 370, 453, 462, 656
HpyCH4III ACNGT 4 cut(s) 533, 929, 1064, 1214
HpyF10VI GCNNNNNNNGC 4 cut(s) 728, 922, 979, 1127
HpyF3I CTNAG 4 cut(s) 59, 233, 718, 1005
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 4 cut(s) 173, 289, 1022, 1233
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 4 cut(s) 294, 409, 436, 442
Kzo9I GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
LmnI GCTCC 3 cut(s) 499, 887, 1253
LweI GCATC 1 cut(s) 587
MaeI CTAG 4 cut(s) 83, 432, 1046, 1124
MaeIII GTNAC 3 cut(s) 17, 201, 506
MalI GATC 9 cut(s) 75, 125, 165, 296, 348, 411, 438, 444, 487
MbiI CCGCTC 1 cut(s) 817
MboI GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
MboII GAAGA 6 cut(s) 116, 230, 341, 674, 795, 800
MhlI GDGCHC 2 cut(s) 60, 892
MlsI TGGCCA 2 cut(s) 545, 836
MluCI AATT 1 cut(s) 997
MluNI TGGCCA 2 cut(s) 545, 836
Mly113I GGCGCC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 516, 625, 690, 727, 859, 1014, 1219, 1272
Mox20I TGGCCA 2 cut(s) 545, 836
MroXI GAANNNNTTC 1 cut(s) 420
MscI TGGCCA 2 cut(s) 545, 836
MseI TTAA 3 cut(s) 339, 1167, 1209
MslI CAYNNNNRTG 4 cut(s) 402, 405, 930, 1014
Msp20I TGGCCA 2 cut(s) 545, 836
MspA1I CMGCKG 3 cut(s) 313, 692, 1034
MspI CCGG 2 cut(s) 170, 885
MspR9I CCNGG 2 cut(s) 177, 466
MvaI CCWGG 2 cut(s) 177, 466
MwoI GCNNNNNNNGC 4 cut(s) 728, 922, 979, 1127
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 831
NdeI CATATG 1 cut(s) 658
NdeII GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
NlaIV GGNNCC 4 cut(s) 174, 450, 889, 1249
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 17, 506
NsbI TGCGCA 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 930
PagI TCATGA 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 223
Pfl23II CGTACG 1 cut(s) 475
PluTI GGCGCC 1 cut(s) 176
PshAI GACNNNNGTC 1 cut(s) 513
PshBI ATTAAT 2 cut(s) 339, 1167
PsiI TTATAA 2 cut(s) 963, 977
Psp6I CCWGG 2 cut(s) 175, 464
PspGI CCWGG 2 cut(s) 175, 464
PspLI CGTACG 1 cut(s) 475
PspN4I GGNNCC 4 cut(s) 174, 450, 889, 1249
PspPI GGNCC 3 cut(s) 40, 247, 462
PstI CTGCAG 2 cut(s) 691, 1090
PstNI CAGNNNCTG 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 313, 692
RsaI GTAC 3 cut(s) 477, 1189, 1273
RsaNI GTAC 3 cut(s) 476, 1188, 1272
RseI CAYNNNNRTG 4 cut(s) 402, 405, 930, 1014
SaqAI TTAA 3 cut(s) 339, 1167, 1209
Sau3AI GATC 9 cut(s) 73, 123, 163, 294, 346, 409, 436, 442, 485
Sau96I GGNCC 3 cut(s) 40, 247, 462
ScrFI CCNGG 2 cut(s) 177, 466
SduI GDGCHC 2 cut(s) 60, 892
SfaNI GCATC 1 cut(s) 587
SfcI CTRYAG 4 cut(s) 687, 789, 1086, 1116
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 40, 247
SmiMI CAYNNNNRTG 4 cut(s) 402, 405, 930, 1014
SmlI CTYRAG 1 cut(s) 1138
SmoI CTYRAG 1 cut(s) 1138
Sse9I AATT 1 cut(s) 997
SsiI CCGC 3 cut(s) 815, 971, 1034
SspDI GGCGCC 1 cut(s) 172
SspI AATATT 1 cut(s) 1165
SspMI CTAG 4 cut(s) 83, 432, 1046, 1124
StyD4I CCNGG 2 cut(s) 175, 464
StyI CCWWGG 2 cut(s) 546, 831
TaaI ACNGT 4 cut(s) 533, 929, 1064, 1214
TaqI TCGA 2 cut(s) 72, 1067
TasI AATT 1 cut(s) 997
TauI GCSGC 2 cut(s) 817, 1037
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 3 cut(s) 339, 1167, 1209
Tru9I TTAA 3 cut(s) 339, 1167, 1209
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 2 cut(s) 17, 506
Tsp45I GTSAC 2 cut(s) 17, 506
TspDTI ATGAA 6 cut(s) 21, 113, 675, 816, 910, 1163
TspGWI ACGGA 2 cut(s) 463, 525
TspRI CASTG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 40, 247
VspI ATTAAT 2 cut(s) 339, 1167
XapI RAATTY 1 cut(s) 997
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 4 cut(s) 83, 432, 1046, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.