Rh3BG305800
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002724
Physical Location & Seq
Forward (+)
30838494 .. 30872537
34044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG305800.1

Sequence Viewer

Length: 1098 bp
ATGGATGAACAACGCTGTGACTTGAAACACTTACACTCGGACCAGAGCTTTCTGGGGCTCAGTAGTAGTTCCGATCATCGTCTAGTTGAATCCCCAATAAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCCTTTCCAATAATAGACTTCCCAATCAACCGGCGCCTGGTCATGCAGAGCTGACCACTCTTCAGCTTGAAGTTGAGTTACAGCGAGAACACGAAGAGAATCAAAAGCTCAGAACCATGTTGGACCAAATCACCAAAAGCTACAAAGGGTTGCAGGCTCAGCTGCGCATGATCAGACTACAAGAGCAGCTGCAAACTTGTGCAGAAGACAGCAATCTTGATCCGAAGTTATCCTTCCTACATGATGATCAATCCCCCAAAAGTAGTTCTCATCACGATCATGATCAAGAACAAGTTCTAGCTGTTACTGATCATGATCAGGTTCCTTTAAGAAGGGCTAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGCGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGGTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGAATGGCAGAGGACAAGACCATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCTGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCAGCGCCCCTAACCCTAACCCTAATCCCATGCAGTTCATGCACAGTAGTGCTACATTTCCTATTCCTTTGCAGGGTTATAATCATCCGCTTATAAAGCACCCCATGTGTTTGAATATCCCTCGGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGATCTGCAGCCATTTCTAGAACAACCCCACGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

365

Amino Acids

40.85

Weight (kDa)

6.61

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 174 - 231 1.3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 975, 989
Acc16I TGCGCA 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 829
AciI CCGC 3 cut(s) 827, 983, 1046
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 555, 846
AcuI CTGAAG 2 cut(s) 185, 806
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 1 cut(s) 489
AfeI AGCGCT 1 cut(s) 1035
AfiI CCNNNNNNNGG 3 cut(s) 176, 833, 968
AgsI TTSAA 7 cut(s) 25, 89, 118, 209, 762, 858, 1009
AjnI CCWGG 1 cut(s) 175
AleI CACNNNNGTG 1 cut(s) 942
AloI GAACNNNNNNTCC 2 cut(s) 600, 632
Alw26I GTCTC 1 cut(s) 487
AlwI GGATC 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 1035
AoxI GGCC 2 cut(s) 555, 846
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 4 cut(s) 175, 306, 902, 1036
AspS9I GGNCC 2 cut(s) 40, 262
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 40, 262
BalI TGGCCA 2 cut(s) 557, 848
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BbsI GAAGAC 1 cut(s) 351
BccI CCATC 2 cut(s) 515, 547
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 5 cut(s) 309, 385, 421, 448, 454
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 5 cut(s) 83, 437, 477, 1058, 1079
BfmI CTRYAG 3 cut(s) 699, 801, 1067
BfoI RGCGCY 3 cut(s) 176, 903, 1037
BglII AGATCT 1 cut(s) 1063
BlpI GCTNAGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 40, 262
BmgT120I GGNCC 2 cut(s) 40, 262
BmiI GGNNCC 2 cut(s) 174, 462
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 297
BsaBI GATNNNNATC 2 cut(s) 420, 453
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 4 cut(s) 558, 843, 1016, 1090
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 833, 968
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 2 cut(s) 420, 453
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 4 cut(s) 558, 843, 1016, 1090
BseGI GGATG 5 cut(s) 10, 530, 614, 839, 979
BseJI GATNNNNATC 2 cut(s) 420, 453
BseLI CCNNNNNNNGG 3 cut(s) 176, 833, 968
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 3 cut(s) 73, 262, 311
BseNI ACTGG 1 cut(s) 891
BsgI GTGCAG 3 cut(s) 360, 577, 849
BshFI GGCC 2 cut(s) 557, 848
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 170
BsiWI CGTACG 1 cut(s) 487
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 3 cut(s) 176, 833, 968
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 2 cut(s) 557, 848
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 60
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 3 cut(s) 827, 983, 1046
BspANI GGCC 2 cut(s) 557, 848
BspCNI CTCAG 3 cut(s) 72, 261, 310
BspHI TCATGA 2 cut(s) 418, 451
BspLI GGNNCC 2 cut(s) 174, 462
BspMAI CTGCAG 2 cut(s) 703, 1071
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 829
BsrDI GCAATG 1 cut(s) 1056
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 891
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 558, 843, 1016, 1090
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 558, 843
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 3 cut(s) 545, 941, 1094
Bst6I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
BstACI GRCGYC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 934
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 3 cut(s) 59, 248, 297
BstDSI CCRYGG 2 cut(s) 843, 1090
BstF5I GGATG 5 cut(s) 10, 530, 614, 839, 979
BstH2I RGCGCY 3 cut(s) 176, 903, 1037
BstHHI GCGC 4 cut(s) 175, 306, 902, 1036
BstMAI GTCTC 1 cut(s) 487
BstMWI GCNNNNNNNGC 4 cut(s) 298, 740, 934, 991
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 3 cut(s) 699, 801, 1067
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 1063
BstYI RGATCY 1 cut(s) 1063
BsuRI GGCC 2 cut(s) 557, 848
BtgI CCRYGG 2 cut(s) 843, 1090
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 5 cut(s) 10, 530, 614, 839, 979
BtsIMutI CAGTG 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 294
CciI TCATGA 2 cut(s) 418, 451
CfoI GCGC 4 cut(s) 175, 306, 902, 1036
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 40, 262
Csp6I GTAC 1 cut(s) 488
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 3 cut(s) 59, 248, 297
DinI GGCGCC 1 cut(s) 174
EaeI YGGCCR 2 cut(s) 555, 846
Eam1104I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
EarI CTCTTC 5 cut(s) 204, 228, 699, 820, 825
Eco130I CCWWGG 2 cut(s) 558, 843
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 487
Eco32I GATATC 2 cut(s) 711, 753
Eco47I GGWCC 2 cut(s) 40, 262
Eco47III AGCGCT 1 cut(s) 1035
Eco57I CTGAAG 2 cut(s) 185, 806
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 2 cut(s) 711, 753
EcoT14I CCWWGG 2 cut(s) 558, 843
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 558, 843
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 558
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 5 cut(s) 309, 385, 421, 448, 454
FokI GGATG 5 cut(s) 17, 537, 621, 826, 966
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 5 cut(s) 83, 437, 477, 1058, 1079
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 4 cut(s) 174, 305, 901, 1035
HaeII RGCGCY 3 cut(s) 176, 903, 1037
HaeIII GGCC 2 cut(s) 557, 848
HapII CCGG 1 cut(s) 170
HhaI GCGC 4 cut(s) 175, 306, 902, 1036
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 4 cut(s) 173, 304, 900, 1034
HinP1I GCGC 4 cut(s) 173, 304, 900, 1034
HinfI GANTC 2 cut(s) 89, 238
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 262
Hpy188I TCNGA 8 cut(s) 40, 73, 251, 314, 363, 502, 621, 660
Hpy188III TCNNGA 6 cut(s) 356, 413, 419, 425, 452, 1079
HpyAV CCTTC 3 cut(s) 382, 465, 668
HpyCH4III ACNGT 3 cut(s) 545, 941, 1094
HpyF10VI GCNNNNNNNGC 4 cut(s) 298, 740, 934, 991
HpyF3I CTNAG 3 cut(s) 59, 248, 297
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 4 cut(s) 173, 304, 900, 1034
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 5 cut(s) 309, 385, 421, 448, 454
LmnI GCTCC 1 cut(s) 511
LweI GCATC 1 cut(s) 599
MaeI CTAG 5 cut(s) 83, 437, 477, 1058, 1079
MaeIII GTNAC 3 cut(s) 17, 216, 442
MbiI CCGCTC 1 cut(s) 829
MboII GAAGA 7 cut(s) 116, 191, 245, 356, 686, 807, 812
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 2 cut(s) 557, 848
MluNI TGGCCA 2 cut(s) 557, 848
Mly113I GGCGCC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 5 cut(s) 528, 637, 702, 871, 1026
Mox20I TGGCCA 2 cut(s) 557, 848
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 2 cut(s) 557, 848
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 3 cut(s) 417, 942, 1026
Msp20I TGGCCA 2 cut(s) 557, 848
MspA1I CMGCKG 4 cut(s) 301, 328, 704, 1046
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 298, 740, 934, 991
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 670
NlaIV GGNNCC 2 cut(s) 174, 462
NmeAIII GCCGAG 1 cut(s) 997
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 942
PagI TCATGA 2 cut(s) 418, 451
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 2 cut(s) 89, 238
Pfl23II CGTACG 1 cut(s) 487
PluTI GGCGCC 1 cut(s) 176
PsiI TTATAA 2 cut(s) 975, 989
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 487
PspN4I GGNNCC 2 cut(s) 174, 462
PspPI GGNCC 2 cut(s) 40, 262
PstI CTGCAG 2 cut(s) 703, 1071
PsuI RGATCY 1 cut(s) 1063
PvuII CAGCTG 3 cut(s) 301, 328, 704
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 3 cut(s) 417, 942, 1026
SaqAI TTAA 1 cut(s) 467
Sau96I GGNCC 2 cut(s) 40, 262
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 3 cut(s) 699, 801, 1067
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 40, 262
SmiMI CAYNNNNRTG 3 cut(s) 417, 942, 1026
SsiI CCGC 3 cut(s) 827, 983, 1046
SspDI GGCGCC 1 cut(s) 172
SspMI CTAG 5 cut(s) 83, 437, 477, 1058, 1079
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 2 cut(s) 558, 843
TaaI ACNGT 3 cut(s) 545, 941, 1094
TauI GCSGC 2 cut(s) 829, 1049
TfiI GAWTC 2 cut(s) 89, 238
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 4 cut(s) 21, 687, 828, 922
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 1 cut(s) 891
VpaK11BI GGWCC 2 cut(s) 40, 262
XbaI TCTAGA 1 cut(s) 1078
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 5 cut(s) 83, 437, 477, 1058, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.