Rh3AG270000
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002724
Physical Location & Seq
Forward (+)
30708730 .. 30711068
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG270000.1

Sequence Viewer

Length: 1305 bp
ATGGATGAACAACGCTGTGACTTGAAACACTTGCACTCGGACCAGAGCTTTCTGGGGCTCGGTAGTAGTTCCGATCATCGCCTAGTTGAATCCCCAATAAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCCTTTCCAATAATAGACTTCCCAATCAACCGGCACCTGGTCATGCAGAGCTGACCACTCTTCAGCTTCAAGTTGAGTTACAGCGAGAACACGAAGAGAATCAAAAGCTCAGAACCATGTTGGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTACAAGACCAGCTGCAAACTTGTGCAGAAGACAGCAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCAATCCCCCAAAAGTAGTTCTCATCACGATCACGATCAAGAACAAGTTCTAGCTGTTACTGATCATGATCAGGTTCCTTTAAGAAGGGCTAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGCGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGGTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGATTGGCAGAGGACAAGACCATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCTCCAGCTGCGCTATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCCGCACCCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTAATAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGAACAACCCCACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCATATCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACAAGGAAATACCAATGGTATTAGTTCCCTTAACAGTAATGGGAATGAGGTTTGCGCAGCATTATCTGGCTCCCCACAGCTTCCTCAATCCTGTACCACTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

434

Amino Acids

47.6

Weight (kDa)

6.3

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 174 - 231 2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 305, 1246
AccB1I GGYRCC 2 cut(s) 172, 993
AccBSI CCGCTC 1 cut(s) 829
AciI CCGC 4 cut(s) 827, 898, 983, 1046
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 555, 846
AcsI RAATTY 1 cut(s) 1009
AcuI CTGAAG 2 cut(s) 185, 806
AfaI GTAC 2 cut(s) 489, 1285
AfeI AGCGCT 1 cut(s) 1035
AfiI CCNNNNNNNGG 4 cut(s) 176, 833, 968, 989
AgsI TTSAA 7 cut(s) 25, 89, 118, 209, 762, 858, 1009
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 600, 632
Alw26I GTCTC 2 cut(s) 487, 1082
AlwI GGATC 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 1035
AoxI GGCC 2 cut(s) 555, 846
ApoI RAATTY 1 cut(s) 1009
AseI ATTAAT 1 cut(s) 1179
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 4 cut(s) 306, 709, 1036, 1247
AspS9I GGNCC 2 cut(s) 40, 262
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 40, 262
BaeGI GKGCMC 1 cut(s) 996
BalI TGGCCA 2 cut(s) 557, 848
BanI GGYRCC 2 cut(s) 172, 993
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1017
BccI CCATC 3 cut(s) 515, 547, 1075
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 3 cut(s) 309, 448, 454
BcoDI GTCTC 2 cut(s) 487, 1082
BfaI CTAG 5 cut(s) 83, 437, 477, 1058, 1136
BfmI CTRYAG 3 cut(s) 801, 1098, 1128
BfoI RGCGCY 1 cut(s) 1037
BlpI GCTNAGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 40, 262
BmgT120I GGNCC 2 cut(s) 40, 262
BmiI GGNNCC 4 cut(s) 174, 462, 995, 1261
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 1017
Bpu1102I GCTNAGC 1 cut(s) 297
BsaBI GATNNNNATC 2 cut(s) 420, 453
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 558, 843, 1072
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 833, 968, 989
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 2 cut(s) 420, 453
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 558, 843, 1072
BseGI GGATG 5 cut(s) 10, 530, 614, 839, 979
BseJI GATNNNNATC 2 cut(s) 420, 453
BseLI CCNNNNNNNGG 4 cut(s) 176, 833, 968, 989
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 3 cut(s) 262, 311, 1031
BseNI ACTGG 1 cut(s) 891
BseSI GKGCMC 1 cut(s) 996
BsgI GTGCAG 3 cut(s) 360, 577, 849
BshFI GGCC 2 cut(s) 557, 848
BshNI GGYRCC 2 cut(s) 172, 993
BsiSI CCGG 1 cut(s) 170
BsiWI CGTACG 1 cut(s) 487
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 4 cut(s) 176, 833, 968, 989
BsmAI GTCTC 2 cut(s) 487, 1082
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 2 cut(s) 557, 848
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 60, 996
Bsp143I GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 4 cut(s) 827, 898, 983, 1046
BspANI GGCC 2 cut(s) 557, 848
BspCNI CTCAG 3 cut(s) 261, 310, 1030
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 4 cut(s) 174, 462, 995, 1261
BspMAI CTGCAG 1 cut(s) 1102
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 2 cut(s) 172, 993
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 829
BsrDI GCAATG 1 cut(s) 1056
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 891
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 558, 843, 1072
BssMI GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
BssT1I CCWWGG 2 cut(s) 558, 843
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 4 cut(s) 545, 941, 1076, 1226
Bst6I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 3 cut(s) 248, 297, 1017
BstDSI CCRYGG 2 cut(s) 843, 1072
BstF5I GGATG 5 cut(s) 10, 530, 614, 839, 979
BstH2I RGCGCY 1 cut(s) 1037
BstHHI GCGC 4 cut(s) 306, 709, 1036, 1247
BstKTI GATC 9 cut(s) 76, 126, 312, 361, 418, 424, 451, 457, 500
BstMAI GTCTC 2 cut(s) 487, 1082
BstMBI GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
BstMWI GCNNNNNNNGC 4 cut(s) 298, 740, 991, 1139
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 3 cut(s) 801, 1098, 1128
BstSLI GKGCMC 1 cut(s) 996
BstV2I GAAGAC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 557, 848
BtgI CCRYGG 2 cut(s) 843, 1072
BtgZI GCGATG 2 cut(s) 62, 534
BtsCI GGATG 5 cut(s) 10, 530, 614, 839, 979
BtsIMutI CAGTG 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 294
CciI TCATGA 1 cut(s) 451
CfoI GCGC 4 cut(s) 306, 709, 1036, 1247
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 40, 262
CsiI ACCWGGT 1 cut(s) 175
Csp6I GTAC 2 cut(s) 488, 1284
CviQI GTAC 2 cut(s) 488, 1284
DdeI CTNAG 3 cut(s) 248, 297, 1017
DpnI GATC 9 cut(s) 75, 125, 311, 360, 417, 423, 450, 456, 499
DpnII GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
EaeI YGGCCR 2 cut(s) 555, 846
Eam1104I CTCTTC 5 cut(s) 204, 228, 699, 820, 825
EarI CTCTTC 5 cut(s) 204, 228, 699, 820, 825
Eco130I CCWWGG 2 cut(s) 558, 843
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 487
Eco32I GATATC 1 cut(s) 753
Eco47I GGWCC 2 cut(s) 40, 262
Eco47III AGCGCT 1 cut(s) 1035
Eco57I CTGAAG 2 cut(s) 185, 806
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 1 cut(s) 753
EcoT14I CCWWGG 2 cut(s) 558, 843
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 558, 843
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 558
FauI CCCGC 1 cut(s) 905
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 3 cut(s) 309, 448, 454
FokI GGATG 5 cut(s) 17, 537, 621, 826, 966
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 5 cut(s) 83, 437, 477, 1058, 1136
FspI TGCGCA 2 cut(s) 305, 1246
GlaI GCGC 4 cut(s) 305, 708, 1035, 1246
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 1037
HaeIII GGCC 2 cut(s) 557, 848
HapII CCGG 1 cut(s) 170
HhaI GCGC 4 cut(s) 306, 709, 1036, 1247
Hin6I GCGC 4 cut(s) 304, 707, 1034, 1245
HinP1I GCGC 4 cut(s) 304, 707, 1034, 1245
HinfI GANTC 2 cut(s) 89, 238
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 1125
Hpy188I TCNGA 9 cut(s) 40, 73, 251, 314, 363, 502, 621, 660, 1114
Hpy188III TCNNGA 5 cut(s) 356, 413, 419, 425, 452
Hpy8I GTNNAC 1 cut(s) 1125
HpyAV CCTTC 3 cut(s) 382, 465, 668
HpyCH4III ACNGT 4 cut(s) 545, 941, 1076, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 298, 740, 991, 1139
HpyF3I CTNAG 3 cut(s) 248, 297, 1017
HspAI GCGC 4 cut(s) 304, 707, 1034, 1245
Ksp22I TGATCA 3 cut(s) 309, 448, 454
Kzo9I GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
LmnI GCTCC 2 cut(s) 511, 1265
LweI GCATC 1 cut(s) 599
MabI ACCWGGT 1 cut(s) 175
MaeI CTAG 5 cut(s) 83, 437, 477, 1058, 1136
MaeIII GTNAC 3 cut(s) 17, 216, 442
MalI GATC 9 cut(s) 75, 125, 311, 360, 417, 423, 450, 456, 499
MbiI CCGCTC 1 cut(s) 829
MboI GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
MboII GAAGA 7 cut(s) 116, 191, 245, 356, 686, 807, 812
MhlI GDGCHC 2 cut(s) 60, 996
MlsI TGGCCA 2 cut(s) 557, 848
MluCI AATT 1 cut(s) 1009
MluNI TGGCCA 2 cut(s) 557, 848
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 8 cut(s) 528, 637, 702, 708, 871, 1026, 1231, 1284
Mox20I TGGCCA 2 cut(s) 557, 848
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 2 cut(s) 557, 848
MseI TTAA 3 cut(s) 467, 1179, 1221
MslI CAYNNNNRTG 1 cut(s) 1026
Msp20I TGGCCA 2 cut(s) 557, 848
MspA1I CMGCKG 4 cut(s) 301, 328, 704, 1046
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 298, 740, 991, 1139
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 670
NdeII GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
NlaIV GGNNCC 4 cut(s) 174, 462, 995, 1261
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 2 cut(s) 305, 1246
PagI TCATGA 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 2 cut(s) 89, 238
Pfl23II CGTACG 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 1179
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 174, 462, 995, 1261
PspPI GGNCC 2 cut(s) 40, 262
PstI CTGCAG 1 cut(s) 1102
PvuII CAGCTG 3 cut(s) 301, 328, 704
RsaI GTAC 2 cut(s) 489, 1285
RsaNI GTAC 2 cut(s) 488, 1284
RseI CAYNNNNRTG 1 cut(s) 1026
SaqAI TTAA 3 cut(s) 467, 1179, 1221
Sau3AI GATC 9 cut(s) 73, 123, 309, 358, 415, 421, 448, 454, 497
Sau96I GGNCC 2 cut(s) 40, 262
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 60, 996
SexAI ACCWGGT 1 cut(s) 175
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 3 cut(s) 801, 1098, 1128
SinI GGWCC 2 cut(s) 40, 262
SmiMI CAYNNNNRTG 1 cut(s) 1026
Sse9I AATT 1 cut(s) 1009
SsiI CCGC 4 cut(s) 827, 898, 983, 1046
SspI AATATT 1 cut(s) 1177
SspMI CTAG 5 cut(s) 83, 437, 477, 1058, 1136
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 2 cut(s) 558, 843
TaaI ACNGT 4 cut(s) 545, 941, 1076, 1226
TaqI TCGA 1 cut(s) 1079
TasI AATT 1 cut(s) 1009
TauI GCSGC 2 cut(s) 829, 1049
TfiI GAWTC 2 cut(s) 89, 238
Tru1I TTAA 3 cut(s) 467, 1179, 1221
Tru9I TTAA 3 cut(s) 467, 1179, 1221
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 21, 687, 828, 922, 1175
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 1 cut(s) 891
VpaK11BI GGWCC 2 cut(s) 40, 262
VspI ATTAAT 1 cut(s) 1179
XapI RAATTY 1 cut(s) 1009
XcmI CCANNNNNNNNNTGG 2 cut(s) 883, 931
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 5 cut(s) 83, 437, 477, 1058, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.