FvH4_1g26200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002724
Physical Location & Seq
Forward (+)
18048575 .. 18052056
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26200.t2

Sequence Viewer

Length: 1278 bp
ATGGATGAACAAGGCTGTGACTTGAAACTAGCTACCGGTAGTAGTTCCGATCATCGTTTGGTTGAAACCCCTGTCAAAGAAGAAACATTACTCAAAGAGATCAAAGAAATTGATTTCTTTCCCAATAGACTTCCCGATCCACCGGCACCTGGTCATCATGTAATGCTGACTACTCTTCAGCTTCAAGTTGAGTTACAGCGAGAACACAAAGAGAATCAAAAGCTCAAATCCATGTTGAACCAAATCACCAAAAGCTACAATGAGTTGCAGGCGCAGCTGCGCATGATCAGACTACAAGAGCAGCTGCAAACTTCTGCAGAAGACGGCTATCTTGATCCGAAGTTATCATCCCTTCATGATGACCAATCCCCCAAAAGTAGTAATCACCAAGATCATGATAAAGAACAAGTTCTAGCTGCTACTGACCATGATCAGGTTCCTTTAAGAAGGGCTAGAGTCTCAGTACGAGCAAGATCGGAAGCTCCCATGCTACAGATTATCGATGGATGTCAGTGGAGGAAGTACGGTCAAAAGATGGCCAAGGGAAACCCCTGTCCTCGTTCTTACTATCGCTGCACTATGGCCGCTGGATGCCCTGTTCGAAAACAGGTGCAAAGATTGGCAGAGGACAAGACCATTCTGATAACCACATATGAAGGTAATCACAACCACCCTCTTCCTCCAGCCGCGAGATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGGTATCGGGTTCAACAACCAGCAAGGAAGGAACACAACCAGCTACAGGCTTGTTCTCTTCTCTTCATGAGCAGCCCTTTGGTTCATCCATGGCCACCTTTTCAACCTCTTCACCATTTCCCACAATCACACTGGACATGACCCGCACCCCGATTTCTAACCCTAATCCCATGCAACTCATCATGCACAGTAGTGCTCCCACGTCATTTCCTCTTCCTTTGCAGGGTTATAATAATCATCCGATTATAGGGCATCCCTTGCGTTTGAATTTCCCTCATCCTAATTATGCAGCGGCAATGCAACTAGATCGAAGCCACAGTTCGATGGTTGAGACGATAACTGCAGCCATTACTTCTGACCCCAAGTTCACTACAGCCCTAGCTGCTGCCATCTCAAGTAGTATGAATAATGTAGAGAATATTAAGACTGGATTACAAGGAAGTTCTGATGATACCAGTTCCCTCAACAGTAATGGTAACGAAGTTTACGCTGTATTATCTGGCTCCCCACAGCATCCTAAGTCATGCACCACTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

426

Amino Acids

46.7

Weight (kDa)

6.79

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 223 3.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
Acc16I TGCGCA 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 1 cut(s) 689
AciI CCGC 4 cut(s) 585, 687, 874, 1022
AclWI GGATC 2 cut(s) 131, 329
AcoI YGGCCR 3 cut(s) 537, 582, 822
AcsI RAATTY 1 cut(s) 997
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 465, 524
AfiI CCNNNNNNNGG 5 cut(s) 149, 433, 776, 953, 977
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 7 cut(s) 25, 65, 185, 238, 744, 834, 997
AjiI CACGTC 1 cut(s) 933
AjnI CCWGG 1 cut(s) 148
AleI CACNNNNGTG 1 cut(s) 921
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 463, 1055
AlwI GGATC 2 cut(s) 131, 329
AoxI GGCC 3 cut(s) 537, 582, 822
ApoI RAATTY 1 cut(s) 997
AsiGI ACCGGT 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 2 cut(s) 274, 282
AsuHPI GGTGA 3 cut(s) 238, 377, 834
AsuII TTCGAA 1 cut(s) 601
BalI TGGCCA 2 cut(s) 539, 824
BanI GGYRCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 72, 104
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 928
BccI CCATC 4 cut(s) 497, 529, 1048, 1127
BceAI ACGGC 1 cut(s) 340
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 1 cut(s) 150
BclI TGATCA 2 cut(s) 285, 430
BcoDI GTCTC 2 cut(s) 463, 1055
BfaI CTAG 5 cut(s) 29, 413, 453, 1034, 1109
BfmI CTRYAG 5 cut(s) 315, 491, 774, 1071, 1101
BglII AGATCT 1 cut(s) 692
Bme1390I CCNGG 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 933
BmiI GGNNCC 3 cut(s) 147, 438, 1234
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 3 cut(s) 581, 991, 1252
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 1 cut(s) 1108
Bsa29I ATCGAT 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 540, 819
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 433, 776, 953, 977
Bse118I RCCGGY 2 cut(s) 35, 142
Bse1I ACTGG 3 cut(s) 867, 1162, 1185
Bse3DI GCAATG 1 cut(s) 1032
BseBI CCWGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 2 cut(s) 540, 819
BseGI GGATG 9 cut(s) 10, 347, 512, 596, 815, 967, 982, 1006, 1243
BseLI CCNNNNNNNGG 5 cut(s) 149, 433, 776, 953, 977
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 3 cut(s) 867, 1162, 1185
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 1 cut(s) 689
BshFI GGCC 3 cut(s) 539, 584, 824
BshNI GGYRCC 1 cut(s) 145
BshTI ACCGGT 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 501
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 2 cut(s) 36, 143
BslI CCNNNNNNNGG 5 cut(s) 149, 433, 776, 953, 977
BsmAI GTCTC 2 cut(s) 463, 1055
BsmBI CGTCTC 1 cut(s) 1055
BsnI GGCC 3 cut(s) 539, 584, 824
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 928
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 585, 687, 874, 1022
BspANI GGCC 3 cut(s) 539, 584, 824
BspCNI CTCAG 1 cut(s) 473
BspDI ATCGAT 1 cut(s) 501
BspFNI CGCG 1 cut(s) 689
BspHI TCATGA 3 cut(s) 355, 394, 796
BspLI GGNNCC 3 cut(s) 147, 438, 1234
BspMAI CTGCAG 2 cut(s) 319, 1075
BspPI GGATC 2 cut(s) 131, 329
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 1032
BsrFI RCCGGY 2 cut(s) 35, 142
BsrI ACTGG 3 cut(s) 867, 1162, 1185
BssAI RCCGGY 2 cut(s) 35, 142
BssECI CCNNGG 2 cut(s) 540, 819
BssT1I CCWWGG 2 cut(s) 540, 819
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 4 cut(s) 527, 920, 1049, 1199
Bst6I CTCTTC 6 cut(s) 180, 681, 793, 798, 844, 948
BstAPI GCANNNNNTGC 1 cut(s) 988
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 460, 1248
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 9 cut(s) 10, 347, 512, 596, 815, 967, 982, 1006, 1243
BstFNI CGCG 1 cut(s) 689
BstHHI GCGC 2 cut(s) 274, 282
BstMAI GTCTC 2 cut(s) 463, 1055
BstMWI GCNNNNNNNGC 4 cut(s) 274, 722, 988, 1112
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 5 cut(s) 315, 491, 774, 1071, 1101
BstUI CGCG 1 cut(s) 689
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
Bsu15I ATCGAT 1 cut(s) 501
BsuRI GGCC 3 cut(s) 539, 584, 824
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 933
BtsCI GGATG 9 cut(s) 10, 347, 512, 596, 815, 967, 982, 1006, 1243
BtsIMutI CAGTG 2 cut(s) 518, 860
Cac8I GCNNGC 1 cut(s) 270
CciI TCATGA 3 cut(s) 355, 394, 796
CfoI GCGC 2 cut(s) 274, 282
Cfr10I RCCGGY 2 cut(s) 35, 142
ClaI ATCGAT 1 cut(s) 501
CsiI ACCWGGT 1 cut(s) 148
Csp6I GTAC 2 cut(s) 464, 523
CspAI ACCGGT 1 cut(s) 35
CviQI GTAC 2 cut(s) 464, 523
DdeI CTNAG 2 cut(s) 460, 1248
EaeI YGGCCR 3 cut(s) 537, 582, 822
Eam1104I CTCTTC 6 cut(s) 180, 681, 793, 798, 844, 948
EarI CTCTTC 6 cut(s) 180, 681, 793, 798, 844, 948
Eco130I CCWWGG 2 cut(s) 540, 819
Eco57I CTGAAG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 148
EcoT14I CCWWGG 2 cut(s) 540, 819
ErhI CCWWGG 2 cut(s) 540, 819
Esp3I CGTCTC 1 cut(s) 1055
FauI CCCGC 1 cut(s) 881
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 2 cut(s) 285, 430
FokI GGATG 9 cut(s) 17, 334, 519, 603, 802, 954, 969, 993, 1230
FspBI CTAG 5 cut(s) 29, 413, 453, 1034, 1109
FspI TGCGCA 1 cut(s) 281
GlaI GCGC 2 cut(s) 273, 281
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 3 cut(s) 539, 584, 824
HapII CCGG 2 cut(s) 36, 143
HhaI GCGC 2 cut(s) 274, 282
Hin6I GCGC 2 cut(s) 272, 280
HinP1I GCGC 2 cut(s) 272, 280
HinfI GANTC 2 cut(s) 214, 456
HpaII CCGG 2 cut(s) 36, 143
HphI GGTGA 3 cut(s) 238, 377, 834
Hpy166II GTNNAC 2 cut(s) 1098, 1216
Hpy188I TCNGA 8 cut(s) 49, 290, 339, 478, 642, 972, 1087, 1177
Hpy188III TCNNGA 5 cut(s) 134, 332, 356, 395, 797
Hpy8I GTNNAC 2 cut(s) 1098, 1216
HpyAV CCTTC 4 cut(s) 362, 441, 650, 752
HpyCH4III ACNGT 4 cut(s) 527, 920, 1049, 1199
HpyCH4IV ACGT 1 cut(s) 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 722, 988, 1112
HpyF3I CTNAG 2 cut(s) 460, 1248
HpySE526I ACGT 1 cut(s) 932
HspAI GCGC 2 cut(s) 272, 280
Ksp22I TGATCA 2 cut(s) 285, 430
LmnI GCTCC 3 cut(s) 487, 931, 1238
LweI GCATC 3 cut(s) 581, 991, 1252
MabI ACCWGGT 1 cut(s) 148
MaeI CTAG 5 cut(s) 29, 413, 453, 1034, 1109
MaeII ACGT 1 cut(s) 932
MaeIII GTNAC 3 cut(s) 17, 192, 1205
MboII GAAGA 8 cut(s) 92, 167, 332, 668, 780, 785, 831, 935
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 928
MlsI TGGCCA 2 cut(s) 539, 824
MluCI AATT 3 cut(s) 108, 997, 1012
MluNI TGGCCA 2 cut(s) 539, 824
MlyI GAGTC 1 cut(s) 465
MnlI CCTC 9 cut(s) 510, 567, 619, 684, 690, 847, 951, 1014, 1202
Mox20I TGGCCA 2 cut(s) 539, 824
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 2 cut(s) 539, 824
MseI TTAA 2 cut(s) 443, 1152
MslI CAYNNNNRTG 1 cut(s) 921
Msp20I TGGCCA 2 cut(s) 539, 824
MspA1I CMGCKG 4 cut(s) 277, 304, 587, 1022
MspI CCGG 2 cut(s) 36, 143
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 689
MwoI GCNNNNNNNGC 4 cut(s) 274, 722, 988, 1112
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 652
NlaIV GGNNCC 3 cut(s) 147, 438, 1234
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 1 cut(s) 281
NspV TTCGAA 1 cut(s) 601
OliI CACNNNNGTG 1 cut(s) 921
PagI TCATGA 3 cut(s) 355, 394, 796
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 214
PinAI ACCGGT 1 cut(s) 35
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 3 cut(s) 147, 438, 1234
PstI CTGCAG 2 cut(s) 319, 1075
PsuI RGATCY 1 cut(s) 692
PvuII CAGCTG 2 cut(s) 277, 304
RsaI GTAC 2 cut(s) 465, 524
RsaNI GTAC 2 cut(s) 464, 523
RseI CAYNNNNRTG 1 cut(s) 921
SaqAI TTAA 2 cut(s) 443, 1152
SchI GAGTC 1 cut(s) 465
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 928
SexAI ACCWGGT 1 cut(s) 148
SfaNI GCATC 3 cut(s) 581, 991, 1252
SfcI CTRYAG 5 cut(s) 315, 491, 774, 1071, 1101
SfuI TTCGAA 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 921
SmlI CTYRAG 1 cut(s) 1123
SmoI CTYRAG 1 cut(s) 1123
Sse9I AATT 3 cut(s) 108, 997, 1012
SsiI CCGC 4 cut(s) 585, 687, 874, 1022
SspI AATATT 1 cut(s) 1150
SspMI CTAG 5 cut(s) 29, 413, 453, 1034, 1109
StyD4I CCNGG 1 cut(s) 148
StyI CCWWGG 2 cut(s) 540, 819
TaaI ACNGT 4 cut(s) 527, 920, 1049, 1199
TaiI ACGT 1 cut(s) 935
TaqI TCGA 4 cut(s) 501, 601, 1039, 1052
TasI AATT 3 cut(s) 108, 997, 1012
TauI GCSGC 3 cut(s) 587, 689, 1025
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 2 cut(s) 443, 1152
Tru9I TTAA 2 cut(s) 443, 1152
TscAI CASTG 2 cut(s) 518, 867
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 6 cut(s) 21, 344, 669, 785, 804, 1148
TspRI CASTG 2 cut(s) 518, 867
XapI RAATTY 1 cut(s) 997
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 5 cut(s) 29, 413, 453, 1034, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.