RLG00000023129
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002724
Physical Location & Seq
Reverse (-)
18603511 .. 18605983
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023129

Sequence Viewer

Length: 1053 bp
ATGTTCGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCAGATGTGCATGATCCGACTTCAAGAGCAGCTGCAAACTTGTGCAGAAGACAGCATTAATCTTGATCCGAAGTTATCCTTCCTACATGATGACCAATCCCCCAAAAGTAGTTCTCATCACGATGATGATCAAGAACAAGTTCTTGCTGGTACTGATCATGATCAGGTTCCTTCAAGAAGGGCCAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGCGACGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACCATCGCTGCAGTATGTTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGAATGGCAGAGGACCAGACCATTCTGATAACAACATATGAAGGTAATCACAACCACCCTCTTCCTGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCTGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCTATGGCCACATTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCGGCGCCCCTAACCCTAACCCTAATCCCTTGCAGTTCATGCACTGTAGTGCTACATTTCCTATTCGTTTGCAGGGTTATAATCATCCGCTTATAAAGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGAACAACCCAACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACTTCTGACACGAAGTTCACTACAGCCCTAGCTGCTGCCATCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACAAGGAAGTACCAATGATATTAGTACCCTTAACAGTAATGGGAATGAGGTTTGCGCTGCATTATCTGGCTCCCCACAGCTTCCTCAATCCTGTACCAGTTTCTCCACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

351

Amino Acids

37.9

Weight (kDa)

6.58

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 90 - 147 4.1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 723, 737
AccB1I GGYRCC 1 cut(s) 647
AccBSI CCGCTC 1 cut(s) 577
AciI CCGC 3 cut(s) 575, 731, 794
AclWI GGATC 2 cut(s) 49, 101
AcoI YGGCCR 2 cut(s) 303, 594
AcsI RAATTY 1 cut(s) 757
AcuI CTGAAG 1 cut(s) 554
AcyI GRCGYC 1 cut(s) 648
AfaI GTAC 5 cut(s) 193, 237, 949, 964, 1033
AfeI AGCGCT 1 cut(s) 783
AfiI CCNNNNNNNGG 1 cut(s) 581
AgsI TTSAA 5 cut(s) 65, 216, 510, 606, 757
AjnI CCWGG 1 cut(s) 224
AleI CACNNNNGTG 1 cut(s) 690
AloI GAACNNNNNNTCC 2 cut(s) 348, 380
AluBI AGCT 7 cut(s) 24, 73, 254, 452, 527, 887, 1018
AluI AGCT 7 cut(s) 24, 73, 254, 452, 527, 887, 1018
Alw26I GTCTC 2 cut(s) 235, 830
AlwI GGATC 2 cut(s) 49, 101
Aor51HI AGCGCT 1 cut(s) 783
AoxI GGCC 3 cut(s) 222, 303, 594
ApoI RAATTY 1 cut(s) 757
AseI ATTAAT 2 cut(s) 99, 927
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 3 cut(s) 650, 784, 995
AspS9I GGNCC 2 cut(s) 222, 394
AsuHPI GGTGA 1 cut(s) 7
AvaII GGWCC 1 cut(s) 394
BalI TGGCCA 2 cut(s) 305, 596
BanI GGYRCC 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 765
BccI CCATC 4 cut(s) 295, 342, 823, 902
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 1 cut(s) 226
BclI TGATCA 3 cut(s) 169, 196, 202
BcoDI GTCTC 2 cut(s) 235, 830
BfaI CTAG 2 cut(s) 806, 884
BfmI CTRYAG 6 cut(s) 340, 447, 549, 688, 846, 876
BfoI RGCGCY 2 cut(s) 651, 785
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 222, 394
BmiI GGNNCC 3 cut(s) 210, 649, 1009
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 347
BoxI GACNNNNGTC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 883
BsaBI GATNNNNATC 2 cut(s) 168, 201
BsaHI GRCGYC 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 225, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 2 cut(s) 639, 1035
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 2 cut(s) 168, 201
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 225, 306
BseGI GGATG 3 cut(s) 278, 362, 727
BseJI GATNNNNATC 2 cut(s) 168, 201
BseLI CCNNNNNNNGG 1 cut(s) 581
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 56, 779
BseNI ACTGG 2 cut(s) 639, 1035
BsgI GTGCAG 2 cut(s) 105, 597
BshFI GGCC 3 cut(s) 224, 305, 596
BshNI GGYRCC 1 cut(s) 647
BsiSI CCGG 1 cut(s) 645
BsiWI CGTACG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 581
BsmAI GTCTC 2 cut(s) 235, 830
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 3 cut(s) 224, 305, 596
Bso31I GGTCTC 1 cut(s) 235
Bsp143I GATC 6 cut(s) 54, 106, 169, 196, 202, 245
BspACI CCGC 3 cut(s) 575, 731, 794
BspANI GGCC 3 cut(s) 224, 305, 596
BspCNI CTCAG 2 cut(s) 55, 778
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 3 cut(s) 210, 649, 1009
BspMAI CTGCAG 3 cut(s) 344, 451, 850
BspPI GGATC 2 cut(s) 49, 101
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 2 cut(s) 639, 1035
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 2 cut(s) 225, 306
BssMI GATC 6 cut(s) 54, 106, 169, 196, 202, 245
BssNI GRCGYC 1 cut(s) 648
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 4 cut(s) 293, 689, 824, 974
Bst6I CTCTTC 3 cut(s) 447, 568, 573
BstACI GRCGYC 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 42, 765
BstF5I GGATG 3 cut(s) 278, 362, 727
BstH2I RGCGCY 2 cut(s) 651, 785
BstHHI GCGC 3 cut(s) 650, 784, 995
BstKTI GATC 6 cut(s) 57, 109, 172, 199, 205, 248
BstMAI GTCTC 2 cut(s) 235, 830
BstMBI GATC 6 cut(s) 54, 106, 169, 196, 202, 245
BstMWI GCNNNNNNNGC 4 cut(s) 488, 682, 739, 887
BstNI CCWGG 1 cut(s) 226
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 6 cut(s) 340, 447, 549, 688, 846, 876
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 224, 305, 596
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 3 cut(s) 278, 362, 727
BtsIMutI CAGTG 2 cut(s) 632, 685
Cac8I GCNNGC 1 cut(s) 39
CciI TCATGA 1 cut(s) 199
CfoI GCGC 3 cut(s) 650, 784, 995
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 222, 394
Csp6I GTAC 5 cut(s) 192, 236, 948, 963, 1032
CviAII CATG 9 cut(s) 52, 128, 200, 259, 493, 640, 682, 748, 776
CviQI GTAC 5 cut(s) 192, 236, 948, 963, 1032
DdeI CTNAG 2 cut(s) 42, 765
DinI GGCGCC 1 cut(s) 649
DpnI GATC 6 cut(s) 56, 108, 171, 198, 204, 247
DpnII GATC 6 cut(s) 54, 106, 169, 196, 202, 245
EaeI YGGCCR 2 cut(s) 303, 594
Eam1104I CTCTTC 3 cut(s) 447, 568, 573
EarI CTCTTC 3 cut(s) 447, 568, 573
Eco130I CCWWGG 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 235
Eco32I GATATC 2 cut(s) 459, 501
Eco47I GGWCC 1 cut(s) 394
Eco47III AGCGCT 1 cut(s) 783
Eco57I CTGAAG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 224
EcoRV GATATC 2 cut(s) 459, 501
EcoT14I CCWWGG 1 cut(s) 306
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 9 cut(s) 55, 131, 203, 262, 496, 643, 685, 751, 779
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FaqI GGGAC 1 cut(s) 306
FatI CATG 9 cut(s) 51, 127, 199, 258, 492, 639, 681, 747, 775
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 3 cut(s) 169, 196, 202
FokI GGATG 3 cut(s) 285, 369, 714
FspBI CTAG 2 cut(s) 806, 884
GlaI GCGC 3 cut(s) 649, 783, 994
HaeII RGCGCY 2 cut(s) 651, 785
HaeIII GGCC 3 cut(s) 224, 305, 596
HapII CCGG 1 cut(s) 645
HhaI GCGC 3 cut(s) 650, 784, 995
Hin1I GRCGYC 1 cut(s) 648
Hin1II CATG 9 cut(s) 55, 131, 203, 262, 496, 643, 685, 751, 779
Hin6I GCGC 3 cut(s) 648, 782, 993
HinP1I GCGC 3 cut(s) 648, 782, 993
HpaII CCGG 1 cut(s) 645
HphI GGTGA 1 cut(s) 7
Hpy166II GTNNAC 1 cut(s) 873
Hpy188I TCNGA 7 cut(s) 45, 59, 111, 250, 369, 408, 862
Hpy188III TCNNGA 6 cut(s) 65, 104, 161, 173, 200, 216
Hpy8I GTNNAC 1 cut(s) 873
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 4 cut(s) 130, 213, 222, 416
HpyCH4III ACNGT 4 cut(s) 293, 689, 824, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 488, 682, 739, 887
HpyF3I CTNAG 2 cut(s) 42, 765
Hsp92I GRCGYC 1 cut(s) 648
Hsp92II CATG 9 cut(s) 55, 131, 203, 262, 496, 643, 685, 751, 779
HspAI GCGC 3 cut(s) 648, 782, 993
KasI GGCGCC 1 cut(s) 647
Ksp22I TGATCA 3 cut(s) 169, 196, 202
Kzo9I GATC 6 cut(s) 54, 106, 169, 196, 202, 245
LmnI GCTCC 2 cut(s) 259, 1013
LweI GCATC 1 cut(s) 347
MaeI CTAG 2 cut(s) 806, 884
MalI GATC 6 cut(s) 56, 108, 171, 198, 204, 247
MbiI CCGCTC 1 cut(s) 577
MboI GATC 6 cut(s) 54, 106, 169, 196, 202, 245
MboII GAAGA 4 cut(s) 101, 434, 555, 560
MlsI TGGCCA 2 cut(s) 305, 596
MluCI AATT 1 cut(s) 757
MluNI TGGCCA 2 cut(s) 305, 596
Mly113I GGCGCC 1 cut(s) 648
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 7 cut(s) 276, 385, 450, 619, 774, 979, 1032
Mox20I TGGCCA 2 cut(s) 305, 596
MroXI GAANNNNTTC 1 cut(s) 180
MscI TGGCCA 2 cut(s) 305, 596
MseI TTAA 3 cut(s) 99, 927, 969
MslI CAYNNNNRTG 4 cut(s) 162, 165, 690, 774
Msp20I TGGCCA 2 cut(s) 305, 596
MspA1I CMGCKG 3 cut(s) 73, 452, 794
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 4 cut(s) 488, 682, 739, 887
NarI GGCGCC 1 cut(s) 648
NdeI CATATG 1 cut(s) 418
NdeII GATC 6 cut(s) 54, 106, 169, 196, 202, 245
NlaIII CATG 9 cut(s) 55, 131, 203, 262, 496, 643, 685, 751, 779
NlaIV GGNNCC 3 cut(s) 210, 649, 1009
OliI CACNNNNGTG 1 cut(s) 690
PagI TCATGA 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 180
Pfl23II CGTACG 1 cut(s) 235
PluTI GGCGCC 1 cut(s) 651
PshAI GACNNNNGTC 1 cut(s) 273
PshBI ATTAAT 2 cut(s) 99, 927
PsiI TTATAA 2 cut(s) 723, 737
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspLI CGTACG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 210, 649, 1009
PspPI GGNCC 2 cut(s) 222, 394
PstI CTGCAG 3 cut(s) 344, 451, 850
PvuII CAGCTG 2 cut(s) 73, 452
RsaI GTAC 5 cut(s) 193, 237, 949, 964, 1033
RsaNI GTAC 5 cut(s) 192, 236, 948, 963, 1032
RseI CAYNNNNRTG 4 cut(s) 162, 165, 690, 774
SaqAI TTAA 3 cut(s) 99, 927, 969
Sau3AI GATC 6 cut(s) 54, 106, 169, 196, 202, 245
Sau96I GGNCC 2 cut(s) 222, 394
ScrFI CCNGG 1 cut(s) 226
SfaNI GCATC 1 cut(s) 347
SfcI CTRYAG 6 cut(s) 340, 447, 549, 688, 846, 876
SfoI GGCGCC 1 cut(s) 649
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 4 cut(s) 162, 165, 690, 774
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
Sse9I AATT 1 cut(s) 757
SsiI CCGC 3 cut(s) 575, 731, 794
SspDI GGCGCC 1 cut(s) 647
SspI AATATT 1 cut(s) 925
SspMI CTAG 2 cut(s) 806, 884
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 293, 689, 824, 974
TaqI TCGA 2 cut(s) 6, 827
TasI AATT 1 cut(s) 757
TauI GCSGC 2 cut(s) 577, 797
Tru1I TTAA 3 cut(s) 99, 927, 969
Tru9I TTAA 3 cut(s) 99, 927, 969
TscAI CASTG 2 cut(s) 639, 692
TspDTI ATGAA 4 cut(s) 435, 576, 670, 923
TspGWI ACGGA 2 cut(s) 223, 285
TspRI CASTG 2 cut(s) 639, 692
VpaK11BI GGWCC 1 cut(s) 394
VspI ATTAAT 2 cut(s) 99, 927
XapI RAATTY 1 cut(s) 757
XcmI CCANNNNNNNNNTGG 2 cut(s) 631, 826
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 806, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.