Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002724

Physical Location & Seq

Genomic Coordinates Forward (+)

33297699 .. 33301391

Gene Structure 3693 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g42870.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCCAAAGGAGGTGGACTCTCCATTGATTCAGATCCAATTGCCTACTTGTCTCTCCACAAACCCATAGTCCTCAACTCCTTCCAAGATCACTACCCTCGTCCCACCTCTACCACCTACCACCACCTCTCTTCTTCTTCCATGGACAACCCTCCTCCTCCTCCTCCGCCGCCGCCCCAGACTCTGCAACTCTCAGTCAACCTCAACTGCACCGACGACCGCGAAAACGACACCCTCCTCAGCTCGCCGCCGCAGTCGGACGACAACCAGCGCAAGGTCGTAGACGAGCGGGACTTCTTCGCCGATAACAACAACAAGGCTGCCGCCGAGGAAGCTTCCGCTGACAAAATGGACTTCGACGACGGCCCTTCCGGCATGGATTTCAACCTTAACACTGGGTTGAATCTTCTTATAACAAACACCAGCAGTGATCAGTCGGTTGTGGATGATGGCATTTCGTCGAGCTTGGAGGATAAAAGAGCTAAAAGTGAGCTGGCGGTTCTTCAAGCTGAGCTGGAGAGGATGAACAGCGAGAATCAAAGGTTAAGAGGGATGCTTAGTCAGGTTACTACCAATTACAATGCTCTACAAGTGCATTTGCTGAGTTTGATGCAAAACCAGAAGGCTGACAACGGTGCGGAAGTGGAAGAAAAGAAGGTTAATACTGGCAATGGATCGCAGGTAGTGGTTCCGAGGCAGTTTATGGATCTTGGTTTGGCTGCAAATGGGGATGCCGAAGAGCCGTCACAGTCTTCGTCGGATGAGAGAAGCCGGGAACGGTCTGGATCAGGAGACGATGGGAAGACTGGTGCGGGGCATGTTGGTGATGAGGGGCATGCGTTTGATCAGGTGAAGAAGGAATTCGGTAGGGGGATTAGTGGGAGAGAGGAGAGTCCTGATCAACAAACGCAGAGTTGGGGACCTAACAAAGTTCCGAGGCTCAATTCTCCAAAGGAGGTTGATCAAGCTGAGGCTACAATGAGGAAGGCTCGGGTTTCTGTAAGAGCTCGATCAGAGGCGACCATGATCACTGATGGATGTCAATGGCGCAAGTATGGGCAAAAGATGGCGAAAGGAAACCCATGTCCTCGAGCTTATTACAGATGCACCATGGCTGCTGGTTGCCCAGTTCGAAAGCAAGTACAAAGATGTGCGGACGATAGGACAATCCTGGTCACAACATATGAAGGCAATCACAACCACCCATTGCCACCAGCCGCCATGGCAATGGCATCAACCACATCATCGGCAGCCCGGATGTTGCTCTCCGGGTCTATGCCAAGCGCCGATGGCCTAATGGACTCTAGCTTCCTCACAAGGACCATCCTCCCATGCTCCTCAAGCATGGCTACAATCTCAGCCTCAGCTCCATTTCCTACTGTCACATTGGACTTAACACAGTCTCCAAACCCGTTACAGTTCCCAAGGCCACCAGGTCAATTTAACATCCCATTCCCGAACCCGTCTCAGACCAACGGACCTGCCTCGTTGCTGCCCCAAATCTTCGGTCAGGCGCTCTACAACCAGTCTAAGTTCTCCGGCCTTCAAATGTCACAGGACATGGACCGTAATGCCCAACCCCAGCAAGCTCAGCAAGGACAGCAGCAGAACTCATTGGCAGATACTGTCAGTGCGGCCACTGCCGCTATCGCAGCTGATCCAACCTTCACTGCAGCTCTAGCAGCCGCCATCACCTCCATCCTTGGAAATGCTCATCCAAACGACATTGGTAACAATTCTACCAACAACAACACAAACAACAACAATAGCAATGGAAACAACAGCAGCAAATTGCAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

601

Amino Acids

64.56

Weight (kDa)

5.2

Isoelectric Point (pI)

46.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	195 - 252	7e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01720
fragaria_vesca	FvH4_1g26200 FvH4_1g26200 FvH4_6g42870 FvH4_6g42870 FvH4_6g42870
malus_domestica	MD09G1111200.v1.1 MD17G1099000.v1.1
prunus_persica	Prupe.3G002300_v2.0.a1 Prupe.3G214800_v2.0.a1
pyrus_communis	pycom09g03370
rosa_chinensis	RchiOBHm_Chr2g0158801 RchiOBHm_Chr3g0485691 RchiOBHm_Chr3g0485711
rosa_laevigata	RLG00000021114 RLG00000023129 RLG00000023130
rosa_multiflora	Rmu_sc0003005.1_g000017 Rmu_sc0003005.1_g000026 Rmu_ssc0000129.1_g000002
rosa_roxburghii	Rroxscaffold_6G00396400 Rroxscaffold_6G00396500 Rroxscaffold_6G00396810 Rroxscaffold_6G00396830
rosa_rugosa	Rorug02G0472600 Rorug03G0220800
rosa_samantha	Rh2AG538400 Rh2BG551100 Rh2CG521600 Rh2DG560900 Rh3AG270000 Rh3AG270200 Rh3BG305800 Rh3CG303900 Rh3CG304200 Rh3DG300200
rosa_wichuraiana	Rw0G006930 Rw0G009910 Rw2G044560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	413
Acc36I	ACCTGC	2 cut(s)	670, 1489
AccBSI	CCGCTC	1 cut(s)	289
AccI	GTMKAC	1 cut(s)	282
AccII	CGCG	1 cut(s)	222
AclWI	GGATC	5 cut(s)	29, 682, 714, 793, 1652
AcoI	YGGCCR	2 cut(s)	3, 1635
AcsI	RAATTY	1 cut(s)	860
AfaI	GTAC	1 cut(s)	1143
AfiI	CCNNNNNNNGG	1 cut(s)	274
AgsI	TTSAA	4 cut(s)	385, 403, 506, 1547
AjnI	CCWGG	2 cut(s)	1170, 1432
Alw21I	GWGCWC	1 cut(s)	1009
Alw26I	GTCTC	4 cut(s)	57, 786, 1407, 1470
AlwI	GGATC	5 cut(s)	29, 682, 714, 793, 1652
AlwNI	CAGNNNCTG	2 cut(s)	184, 1625
Ama87I	CYCGRG	2 cut(s)	990, 1089
AoxI	GGCC	6 cut(s)	3, 364, 1291, 1427, 1540, 1635
ApoI	RAATTY	1 cut(s)	860
ArsI	GACNNNNNNTTYG	4 cut(s)	54, 86, 1492, 1524
Asp700I	GAANNNNTTC	1 cut(s)	860
AspLEI	GCGC	4 cut(s)	273, 1050, 1286, 1516
AspS9I	GGNCC	5 cut(s)	365, 920, 1320, 1478, 1564
AsuC2I	CCSGG	3 cut(s)	773, 1255, 1270
AsuHPI	GGTGA	3 cut(s)	836, 862, 1684
AsuII	TTCGAA	1 cut(s)	1132
AvaI	CYCGRG	2 cut(s)	990, 1089
AvaII	GGWCC	4 cut(s)	920, 1320, 1478, 1564
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	1009
BbsI	GAAGAC	2 cut(s)	744, 809
Bbv12I	GWGCWC	1 cut(s)	1009
BbvCI	CCTCAGC	3 cut(s)	239, 969, 1363
BccI	CCATC	8 cut(s)	443, 791, 1028, 1060, 1283, 1331, 1697, 1706
BceAI	ACGGC	2 cut(s)	379, 727
BciT130I	CCWGG	2 cut(s)	1172, 1434
BclI	TGATCA	5 cut(s)	430, 844, 898, 961, 1026
BcnI	CCSGG	3 cut(s)	773, 1255, 1270
BcoDI	GTCTC	4 cut(s)	57, 786, 1407, 1470
BfaI	CTAG	2 cut(s)	1305, 1679
BfmI	CTRYAG	1 cut(s)	1671
BfoI	RGCGCY	2 cut(s)	1287, 1517
BfuAI	ACCTGC	2 cut(s)	670, 1489
BglI	GCCNNNNNGGC	2 cut(s)	372, 1223
BlpI	GCTNAGC	2 cut(s)	510, 1590
Bme1390I	CCNGG	5 cut(s)	773, 1172, 1255, 1270, 1434
Bme18I	GGWCC	4 cut(s)	920, 1320, 1478, 1564
BmeT110I	CYCGRG	2 cut(s)	990, 1089
BmgT120I	GGNCC	5 cut(s)	365, 920, 1320, 1478, 1564
BmiI	GGNNCC	2 cut(s)	690, 921
BmrFI	CCNGG	5 cut(s)	773, 1172, 1255, 1270, 1434
BmrI	ACTGGG	2 cut(s)	405, 1121
BmsI	GCATC	5 cut(s)	543, 600, 721, 1094, 1241
BmuI	ACTGGG	2 cut(s)	405, 1121
BpiI	GAAGAC	2 cut(s)	744, 809
BplI	GAGNNNNNCTC	3 cut(s)	35, 973, 1005
BpmI	CTGGAG	1 cut(s)	536
Bpu10I	CCTNAGC	3 cut(s)	239, 969, 1363
Bpu1102I	GCTNAGC	2 cut(s)	510, 1590
Bpu14I	TTCGAA	1 cut(s)	1132
BpuEI	CTTGAG	1 cut(s)	1324
BpuMI	CCSGG	3 cut(s)	773, 1255, 1270
BsaBI	GATNNNNATC	1 cut(s)	33
BsaJI	CCNNGG	8 cut(s)	141, 327, 692, 935, 1110, 1221, 1424, 1702
BsaXI	ACNNNNNCTCC	2 cut(s)	1387, 1417
Bsc4I	CCNNNNNNNGG	1 cut(s)	274
Bse1I	ACTGG	5 cut(s)	400, 670, 811, 1127, 1525
Bse3DI	GCAATG	4 cut(s)	676, 1205, 1233, 1777
Bse8I	GATNNNNATC	1 cut(s)	33
BseBI	CCWGG	2 cut(s)	1172, 1434
BseDI	CCNNGG	8 cut(s)	141, 327, 692, 935, 1110, 1221, 1424, 1702
BseJI	GATNNNNATC	1 cut(s)	33
BseLI	CCNNNNNNNGG	1 cut(s)	274
BseMI	GCAATG	4 cut(s)	676, 1205, 1233, 1777
BseMII	CTCAG	9 cut(s)	207, 253, 501, 593, 960, 1371, 1377, 1481, 1604
BseNI	ACTGG	5 cut(s)	400, 670, 811, 1127, 1525
BseRI	GAGGAG	7 cut(s)	144, 147, 150, 153, 227, 902, 1327
BseYI	CCCAGC	1 cut(s)	1581
BsgI	GTGCAG	1 cut(s)	193
Bsh1236I	CGCG	1 cut(s)	222
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	6 cut(s)	5, 366, 1293, 1429, 1542, 1637
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	1 cut(s)	1009
BsiHKCI	CYCGRG	2 cut(s)	990, 1089
BsiSI	CCGG	5 cut(s)	372, 772, 1255, 1269, 1539
BslFI	GGGAC	3 cut(s)	87, 305, 933
BslI	CCNNNNNNNGG	1 cut(s)	274
BsmAI	GTCTC	4 cut(s)	57, 786, 1407, 1470
BsmBI	CGTCTC	2 cut(s)	786, 1470
BsmFI	GGGAC	3 cut(s)	87, 305, 933
BsnI	GGCC	6 cut(s)	5, 366, 1293, 1429, 1542, 1637
BsoBI	CYCGRG	2 cut(s)	990, 1089
Bsp119I	TTCGAA	1 cut(s)	1132
Bsp1286I	GDGCHC	1 cut(s)	1009
Bsp1720I	GCTNAGC	2 cut(s)	510, 1590
Bsp19I	CCATGG	3 cut(s)	141, 1110, 1221
BspANI	GGCC	6 cut(s)	5, 366, 1293, 1429, 1542, 1637
BspCNI	CTCAG	9 cut(s)	206, 252, 502, 594, 961, 1370, 1376, 1480, 1603
BspFNI	CGCG	1 cut(s)	222
BspLI	GGNNCC	2 cut(s)	690, 921
BspMAI	CTGCAG	1 cut(s)	1675
BspMI	ACCTGC	2 cut(s)	670, 1489
BspPI	GGATC	5 cut(s)	29, 682, 714, 793, 1652
BspQI	GCTCTTC	1 cut(s)	732
BspT104I	TTCGAA	1 cut(s)	1132
BsrBI	CCGCTC	1 cut(s)	289
BsrDI	GCAATG	4 cut(s)	676, 1205, 1233, 1777
BsrI	ACTGG	5 cut(s)	400, 670, 811, 1127, 1525
BssECI	CCNNGG	8 cut(s)	141, 327, 692, 935, 1110, 1221, 1424, 1702
BssT1I	CCWWGG	5 cut(s)	141, 1110, 1221, 1424, 1702
Bst2UI	CCWGG	2 cut(s)	1172, 1434
Bst4CI	ACNGT	8 cut(s)	635, 750, 780, 1381, 1401, 1419, 1568, 1627
Bst6I	CTCTTC	2 cut(s)	136, 732
BstBI	TTCGAA	1 cut(s)	1132
BstC8I	GCNNGC	4 cut(s)	245, 495, 837, 1587
BstDSI	CCRYGG	3 cut(s)	141, 1110, 1221
BstFNI	CGCG	1 cut(s)	222
BstH2I	RGCGCY	2 cut(s)	1287, 1517
BstHHI	GCGC	4 cut(s)	273, 1050, 1286, 1516
BstMAI	GTCTC	4 cut(s)	57, 786, 1407, 1470
BstMCI	CGRYCG	1 cut(s)	220
BstNI	CCWGG	2 cut(s)	1172, 1434
BstNSI	RCATGY	2 cut(s)	821, 839
BstSCI	CCNGG	5 cut(s)	771, 1170, 1253, 1268, 1432
BstSFI	CTRYAG	1 cut(s)	1671
BstUI	CGCG	1 cut(s)	222
BstV2I	GAAGAC	2 cut(s)	744, 809
BstX2I	RGATCY	2 cut(s)	34, 706
BstXI	CCANNNNNNTGG	1 cut(s)	1228
BstYI	RGATCY	2 cut(s)	34, 706
BsuRI	GGCC	6 cut(s)	5, 366, 1293, 1429, 1542, 1637
BtgI	CCRYGG	3 cut(s)	141, 1110, 1221
BtsI	GCAGTG	3 cut(s)	433, 1638, 1668
BtsIMutI	CAGTG	6 cut(s)	393, 433, 1029, 1636, 1638, 1668
BveI	ACCTGC	2 cut(s)	670, 1489
Cac8I	GCNNGC	4 cut(s)	245, 495, 837, 1587
CaiI	CAGNNNCTG	2 cut(s)	184, 1625
CfoI	GCGC	4 cut(s)	273, 1050, 1286, 1516
Cfr13I	GGNCC	5 cut(s)	365, 920, 1320, 1478, 1564
CsiI	ACCWGGT	1 cut(s)	1432
Csp6I	GTAC	1 cut(s)	1142
CspCI	CAANNNNNGTGG	1 cut(s)	30
CviQI	GTAC	1 cut(s)	1142
EaeI	YGGCCR	2 cut(s)	3, 1635
Eam1104I	CTCTTC	2 cut(s)	136, 732
EarI	CTCTTC	2 cut(s)	136, 732
EciI	GGCGGA	1 cut(s)	156
Ecl136II	GAGCTC	1 cut(s)	1007
Eco130I	CCWWGG	5 cut(s)	141, 1110, 1221, 1424, 1702
Eco24I	GRGCYC	1 cut(s)	1009
Eco47I	GGWCC	4 cut(s)	920, 1320, 1478, 1564
Eco53kI	GAGCTC	1 cut(s)	1007
Eco88I	CYCGRG	2 cut(s)	990, 1089
EcoICRI	GAGCTC	1 cut(s)	1007
EcoO109I	RGGNCCY	1 cut(s)	920
EcoRI	GAATTC	1 cut(s)	860
EcoRII	CCWGG	2 cut(s)	1170, 1432
EcoT14I	CCWWGG	5 cut(s)	141, 1110, 1221, 1424, 1702
EcoT38I	GRGCYC	1 cut(s)	1009
ErhI	CCWWGG	5 cut(s)	141, 1110, 1221, 1424, 1702
Esp3I	CGTCTC	2 cut(s)	786, 1470
FaqI	GGGAC	3 cut(s)	87, 305, 933
FauI	CCCGC	2 cut(s)	282, 805
FauNDI	CATATG	1 cut(s)	1183
FbaI	TGATCA	5 cut(s)	430, 844, 898, 961, 1026
FblI	GTMKAC	1 cut(s)	282
FriOI	GRGCYC	1 cut(s)	1009
FspBI	CTAG	2 cut(s)	1305, 1679
GlaI	GCGC	4 cut(s)	272, 1049, 1285, 1515
GsaI	CCCAGC	1 cut(s)	1585
GsuI	CTGGAG	1 cut(s)	536
HaeII	RGCGCY	2 cut(s)	1287, 1517
HaeIII	GGCC	6 cut(s)	5, 366, 1293, 1429, 1542, 1637
HapII	CCGG	5 cut(s)	372, 772, 1255, 1269, 1539
HhaI	GCGC	4 cut(s)	273, 1050, 1286, 1516
Hin6I	GCGC	4 cut(s)	271, 1048, 1284, 1514
HinP1I	GCGC	4 cut(s)	271, 1048, 1284, 1514
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HindIII	AAGCTT	1 cut(s)	333
HinfI	GANTC	7 cut(s)	18, 29, 181, 403, 535, 892, 1301
HpaII	CCGG	5 cut(s)	372, 772, 1255, 1269, 1539
HphI	GGTGA	3 cut(s)	836, 862, 1684
Hpy166II	GTNNAC	3 cut(s)	17, 199, 283
Hpy188I	TCNGA	7 cut(s)	34, 259, 693, 760, 936, 1015, 1470
Hpy188III	TCNNGA	4 cut(s)	783, 789, 896, 1456
Hpy8I	GTNNAC	3 cut(s)	17, 199, 283
Hpy99I	CGWCG	5 cut(s)	218, 362, 365, 463, 760
HpyAV	CCTTC	9 cut(s)	91, 378, 616, 649, 850, 979, 1181, 1553, 1675
HpyCH4III	ACNGT	8 cut(s)	635, 750, 780, 1381, 1401, 1419, 1568, 1627
HpyCH4V	TGCA	8 cut(s)	187, 210, 595, 613, 722, 1107, 1673, 1795
HspAI	GCGC	4 cut(s)	271, 1048, 1284, 1514
Ksp22I	TGATCA	5 cut(s)	430, 844, 898, 961, 1026
LguI	GCTCTTC	1 cut(s)	732
LmnI	GCTCC	2 cut(s)	1340, 1372
LweI	GCATC	5 cut(s)	543, 600, 721, 1094, 1241
MabI	ACCWGGT	1 cut(s)	1432
MaeI	CTAG	2 cut(s)	1305, 1679
MaeIII	GTNAC	7 cut(s)	565, 744, 1174, 1381, 1413, 1551, 1730
MbiI	CCGCTC	1 cut(s)	289
MfeI	CAATTG	1 cut(s)	39
MflI	RGATCY	2 cut(s)	34, 706
MhlI	GDGCHC	1 cut(s)	1009
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	7 cut(s)	39, 574, 860, 943, 1439, 1735, 1790
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	12, 175, 901, 1295
MmeI	TCCRAC	3 cut(s)	237, 738, 1685
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	860
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	390, 545, 660, 1394, 1443
MslI	CAYNNNNRTG	2 cut(s)	822, 1226
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	341, 1655
MspI	CCGG	5 cut(s)	372, 772, 1255, 1269, 1539
MspR9I	CCNGG	5 cut(s)	773, 1172, 1255, 1270, 1434
MunI	CAATTG	1 cut(s)	39
MvaI	CCWGG	2 cut(s)	1172, 1434
MvnI	CGCG	1 cut(s)	222
NciI	CCSGG	3 cut(s)	773, 1255, 1270
NcoI	CCATGG	3 cut(s)	141, 1110, 1221
NdeI	CATATG	1 cut(s)	1183
NlaIV	GGNNCC	2 cut(s)	690, 921
NmeAIII	GCCGAG	1 cut(s)	352
NmuCI	GTSAC	4 cut(s)	744, 1174, 1381, 1551
NspI	RCATGY	2 cut(s)	821, 839
NspV	TTCGAA	1 cut(s)	1132
PaeI	GCATGC	1 cut(s)	839
PaeR7I	CTCGAG	1 cut(s)	1089
PciSI	GCTCTTC	1 cut(s)	732
PdmI	GAANNNNTTC	1 cut(s)	860
PfeI	GAWTC	3 cut(s)	29, 403, 535
PleI	GAGTC	4 cut(s)	12, 175, 900, 1295
PpsI	GAGTC	4 cut(s)	12, 175, 900, 1295
PpuMI	RGGWCCY	1 cut(s)	920
PsiI	TTATAA	1 cut(s)	413
Psp124BI	GAGCTC	1 cut(s)	1009
Psp5II	RGGWCCY	1 cut(s)	920
Psp6I	CCWGG	2 cut(s)	1170, 1432
PspFI	CCCAGC	1 cut(s)	1581
PspGI	CCWGG	2 cut(s)	1170, 1432
PspN4I	GGNNCC	2 cut(s)	690, 921
PspPI	GGNCC	5 cut(s)	365, 920, 1320, 1478, 1564
PspPPI	RGGWCCY	1 cut(s)	920
PspXI	VCTCGAGB	1 cut(s)	1089
PstI	CTGCAG	1 cut(s)	1675
PstNI	CAGNNNCTG	2 cut(s)	184, 1625
PsuI	RGATCY	2 cut(s)	34, 706
PvuII	CAGCTG	1 cut(s)	1655
RsaI	GTAC	1 cut(s)	1143
RsaNI	GTAC	1 cut(s)	1142
RseI	CAYNNNNRTG	2 cut(s)	822, 1226
SacI	GAGCTC	1 cut(s)	1009
SapI	GCTCTTC	1 cut(s)	732
SaqAI	TTAA	5 cut(s)	390, 545, 660, 1394, 1443
Sau96I	GGNCC	5 cut(s)	365, 920, 1320, 1478, 1564
SchI	GAGTC	4 cut(s)	12, 175, 901, 1295
ScrFI	CCNGG	5 cut(s)	773, 1172, 1255, 1270, 1434
SduI	GDGCHC	1 cut(s)	1009
SexAI	ACCWGGT	1 cut(s)	1432
SfaNI	GCATC	5 cut(s)	543, 600, 721, 1094, 1241
SfcI	CTRYAG	1 cut(s)	1671
Sfr274I	CTCGAG	1 cut(s)	1089
SfuI	TTCGAA	1 cut(s)	1132
SinI	GGWCC	4 cut(s)	920, 1320, 1478, 1564
SlaI	CTCGAG	1 cut(s)	1089
SmiMI	CAYNNNNRTG	2 cut(s)	822, 1226
SmlI	CTYRAG	2 cut(s)	1089, 1339
SmoI	CTYRAG	2 cut(s)	1089, 1339
SphI	GCATGC	1 cut(s)	839
Sse9I	AATT	7 cut(s)	39, 574, 860, 943, 1439, 1735, 1790
SspMI	CTAG	2 cut(s)	1305, 1679
SstI	GAGCTC	1 cut(s)	1009
StyD4I	CCNGG	5 cut(s)	771, 1170, 1253, 1268, 1432
StyI	CCWWGG	5 cut(s)	141, 1110, 1221, 1424, 1702
TaaI	ACNGT	8 cut(s)	635, 750, 780, 1381, 1401, 1419, 1568, 1627
TaqI	TCGA	5 cut(s)	357, 461, 1009, 1090, 1132
TaqII	GACCGA	1 cut(s)	1496
TasI	AATT	7 cut(s)	39, 574, 860, 943, 1439, 1735, 1790
TatI	WGTACW	1 cut(s)	1141
TauI	GCSGC	9 cut(s)	172, 175, 250, 253, 326, 1220, 1637, 1646, 1688
TfiI	GAWTC	3 cut(s)	29, 403, 535
Tru1I	TTAA	5 cut(s)	390, 545, 660, 1394, 1443
Tru9I	TTAA	5 cut(s)	390, 545, 660, 1394, 1443
TscAI	CASTG	6 cut(s)	400, 433, 1036, 1636, 1645, 1675
TseFI	GTSAC	4 cut(s)	744, 1174, 1381, 1551
Tsp45I	GTSAC	4 cut(s)	744, 1174, 1381, 1551
TspDTI	ATGAA	2 cut(s)	539, 1200
TspGWI	ACGGA	1 cut(s)	1491
TspRI	CASTG	6 cut(s)	400, 433, 1036, 1636, 1645, 1675
VpaK11BI	GGWCC	4 cut(s)	920, 1320, 1478, 1564
XapI	RAATTY	1 cut(s)	860
XceI	RCATGY	2 cut(s)	821, 839
XhoI	CTCGAG	1 cut(s)	1089
XmiI	GTMKAC	1 cut(s)	282
XmnI	GAANNNNTTC	1 cut(s)	860
XspI	CTAG	2 cut(s)	1305, 1679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.