Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002724

Physical Location & Seq

Genomic Coordinates Reverse (-)

8402127 .. 8405223

Gene Structure 3097 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1099000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGCCAAAGGAAGTGGACTCTCCATTGATTCAGATCCATTTTCCTTCTCTCTCCACAACCCTATAGTCCTCAACTCCTTCCAGGAAGACCGATATAACCACCACCACCTCCCACCACCCTCCACCACCAAAAGACAACAACTTCGCCACCACCACTCATCACTCATGCTCATGGACAACTCCACCGCTATTCACCGCCGCTCTCCTCCGCCCCCTCCTCCTCCCACGACTATCCAGTTCGCAGTCAATCTCAACTGCTCCCACGACGACGACGTGCAGCACTCTCCTGGCTCACCACCACCCTCCAACGAGAAACGCAAAGTCATCGACGAGCGGGACTTCTTCGCCGATAGCAAGAACCGCCTTGATCACGAAAAGTCTGTCGTCTCCACAGACCCTGCCGATAAAATGGACTTACATGACCCTACAGACATGGAATTCAACGTAAATACTGGTTTGAATCTTCTCCTTACAAACACTAGTAGTGACCAATCAGTGGTGGACGATGGCATTTCATCCAACAAAGAGGATAAAAGAGCTAAAAGTGAGCTGGCTGTTCTTCAAGCTGAGCTCAAGCGGATGAACGCGGAAAACCATCGGTTGAAGGGGATGCTTAATCAGGTGACCACCAATTACAATGCACTTCAGGTGCATTTGCTGAATTTGATGCAAAGCCAGAAGGCTGATCAGAACGGTAGCGCTGCCGAAGGCCACGGAGTGGCAGTGGAAGAAAAGAAGATTATGAATGGCAATAAAACATCACTAGTGGTGCCGAGGCAGTTCATGGATCTTGGGTTGGCTGCTAACAATGGTGACGCTGATGAGCATTCACAGTCTTCGTCTGATGAACGAAGTCGTGGTGAACAGTCTGGATCGCTTGGAGATAATGTGAAGGCTGCAGGGCACAGCGACGATCAGGGGATCGCTTTTGATCATGAAAAGAAGGATTTCGAGAGAAGGATTCGGAGAGAGGAGAGCCCGGATCAGCCGTCACAGGGTTGGGGGCCTAACAAAGTTCCGAGGCTCAATTCTCCAAAAGAGGTTGACCAAACTGAGGCTACAATGAGGAAGGCTCGGGTTTCGGTTAGAGCTCGATCGGAGGCGCCTATGATCACTGATGGATGTCAATGGCGAAAGTATGGGCAAAAGATGGCGAAAGGAAATCCGTGTCCTCGTGCTTATTATCGATGCACCATGGCTGCTGGTTGCCCCGTTCGAAAACAAGTACAAAGATGTGCAGAGGATAGGACAATCCTCATCACTACATATGAAGGCAATCACAACCACCCATTGCCTCCGGCAGCAATGGCAATGGCATCGACTACATCATCTGCTGCGCGGATGCTGCTCTCGGGGTCTATGCCAAGTGCAGATGGCCTAATGGACTCAAACTTCCTCACCAGGACAATCCTCCCATGCTCCTCCAGCATGGCCACCATCTCTGCCTCGGCCCCATTCCCTACTGTTACATTGGACTTAACACAATCACCAAACCCTTTACAGCAGCTCCAAAGACCACCAGGCCAGTTCAACATTCCGTTCCCAAACCCTAATCAGAATTTCACCAATGGCCCTGCCTCATTGCTGCCTCAAATTTTTAGTCAGGCGCTATACAACCAGTCTAAATTCTCTGGCCTCCAAATGTCACAGGACACAGACTGTGCCGCAGGTCAACAAGGCCACCAATCGCAACCAGGGCAGCAGCAGGGCTCATTGGCGGATCTTACTGCCGCCACCGCTGCCATTGCAGCTGATCCAAACTTCACTGCAGCCCTAGCAGCTGCCATCACCTCAATCATTGGCAATGCTCATCCAAACAACGACGTTGCCAACCCAGCAACCAACTCAAACAACAACAATGCCAATGGCAATGCCAGCAGCAACAGCAATGGCAATTGCAACAATAAACTTAGCAATCCTAGTTTTTCAGGGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

646

Amino Acids

69.96

Weight (kDa)

6.42

Isoelectric Point (pI)

48.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	373 - 430	1.3e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01720
fragaria_vesca	FvH4_1g26200 FvH4_1g26200 FvH4_6g42870 FvH4_6g42870 FvH4_6g42870
malus_domestica	MD09G1111200.v1.1 MD17G1099000.v1.1
prunus_persica	Prupe.3G002300_v2.0.a1 Prupe.3G214800_v2.0.a1
pyrus_communis	pycom09g03370
rosa_chinensis	RchiOBHm_Chr2g0158801 RchiOBHm_Chr3g0485691 RchiOBHm_Chr3g0485711
rosa_laevigata	RLG00000021114 RLG00000023129 RLG00000023130
rosa_multiflora	Rmu_sc0003005.1_g000017 Rmu_sc0003005.1_g000026 Rmu_ssc0000129.1_g000002
rosa_roxburghii	Rroxscaffold_6G00396400 Rroxscaffold_6G00396500 Rroxscaffold_6G00396810 Rroxscaffold_6G00396830
rosa_rugosa	Rorug02G0472600 Rorug03G0220800
rosa_samantha	Rh2AG538400 Rh2BG551100 Rh2CG521600 Rh2DG560900 Rh3AG270000 Rh3AG270200 Rh3BG305800 Rh3CG303900 Rh3CG304200 Rh3DG300200
rosa_wichuraiana	Rw0G006930 Rw0G009910 Rw2G044560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1658
AccB1I	GGYRCC	2 cut(s)	769, 1102
AccB7I	CCANNNNNTGG	2 cut(s)	496, 718
AccBSI	CCGCTC	2 cut(s)	201, 334
AccII	CGCG	2 cut(s)	587, 1339
AclWI	GGATC	7 cut(s)	29, 795, 880, 929, 990, 1728, 1748
AcoI	YGGCCR	2 cut(s)	3, 1431
AcsI	RAATTY	5 cut(s)	437, 661, 1558, 1593, 1625
AcuI	CTGAAG	1 cut(s)	629
AcyI	GRCGYC	1 cut(s)	1103
AdeI	CACNNNGTG	1 cut(s)	718
AfaI	GTAC	1 cut(s)	1227
AfeI	AGCGCT	1 cut(s)	700
AfiI	CCNNNNNNNGG	4 cut(s)	496, 718, 995, 1054
AgsI	TTSAA	5 cut(s)	442, 460, 563, 604, 1531
AhlI	ACTAGT	2 cut(s)	479, 763
AjiI	CACGTC	1 cut(s)	274
AjnI	CCWGG	5 cut(s)	81, 286, 1400, 1519, 1693
AluBI	AGCT	8 cut(s)	539, 550, 566, 571, 1091, 1507, 1751, 1781
AluI	AGCT	8 cut(s)	539, 550, 566, 571, 1091, 1507, 1751, 1781
Alw21I	GWGCWC	2 cut(s)	573, 1093
Alw26I	GTCTC	1 cut(s)	391
AlwI	GGATC	7 cut(s)	29, 795, 880, 929, 990, 1728, 1748
AlwNI	CAGNNNCTG	1 cut(s)	398
Ama87I	CYCGRG	2 cut(s)	1074, 1351
Aor51HI	AGCGCT	1 cut(s)	700
ApoI	RAATTY	5 cut(s)	437, 661, 1558, 1593, 1625
ArsI	GACNNNNNNTTYG	6 cut(s)	126, 158, 366, 398, 1585, 1617
Asp700I	GAANNNNTTC	1 cut(s)	947
AspLEI	GCGC	4 cut(s)	701, 1105, 1339, 1609
AspS9I	GGNCC	3 cut(s)	1004, 1450, 1571
AsuC2I	CCSGG	1 cut(s)	980
AsuHPI	GGTGA	9 cut(s)	185, 285, 634, 824, 872, 1390, 1479, 1555, 1780
AsuII	TTCGAA	1 cut(s)	1216
AvaI	CYCGRG	2 cut(s)	1074, 1351
BaeGI	GKGCMC	1 cut(s)	906
BalI	TGGCCA	2 cut(s)	5, 1433
BanI	GGYRCC	2 cut(s)	769, 1102
BanII	GRGCYC	4 cut(s)	573, 980, 1093, 1712
BauI	CACGAG	1 cut(s)	1173
BbsI	GAAGAC	2 cut(s)	93, 828
Bbv12I	GWGCWC	2 cut(s)	573, 1093
BccI	CCATC	7 cut(s)	500, 603, 1112, 1144, 1367, 1445, 1793
BceAI	ACGGC	1 cut(s)	973
BcgI	CGANNNNNNTGC	4 cut(s)	307, 341, 1299, 1333
BciT130I	CCWGG	5 cut(s)	83, 288, 1402, 1521, 1695
BclI	TGATCA	4 cut(s)	367, 685, 931, 1110
BcnI	CCSGG	1 cut(s)	980
BcoDI	GTCTC	1 cut(s)	391
BcuI	ACTAGT	2 cut(s)	479, 763
BfaI	CTAG	4 cut(s)	480, 764, 1775, 1920
BfmI	CTRYAG	4 cut(s)	63, 426, 897, 1767
BfoI	RGCGCY	3 cut(s)	702, 1106, 1610
BfuAI	ACCTGC	1 cut(s)	1658
BlpI	GCTNAGC	1 cut(s)	567
Bme1390I	CCNGG	6 cut(s)	83, 288, 980, 1402, 1521, 1695
BmeT110I	CYCGRG	2 cut(s)	1074, 1351
BmgBI	CACGTC	1 cut(s)	274
BmgT120I	GGNCC	3 cut(s)	1004, 1450, 1571
BmiI	GGNNCC	4 cut(s)	771, 1005, 1104, 1452
BmrFI	CCNGG	6 cut(s)	83, 288, 980, 1402, 1521, 1695
BmsI	GCATC	5 cut(s)	600, 657, 1178, 1325, 1332
BpiI	GAAGAC	2 cut(s)	93, 828
BplI	GAGNNNNNCTC	2 cut(s)	1057, 1089
BpmI	CTGGAG	1 cut(s)	1408
Bpu1102I	GCTNAGC	1 cut(s)	567
Bpu14I	TTCGAA	1 cut(s)	1216
BpuEI	CTTGAG	1 cut(s)	557
BpuMI	CCSGG	1 cut(s)	980
Bsa29I	ATCGAT	1 cut(s)	1186
BsaBI	GATNNNNATC	1 cut(s)	33
BsaHI	GRCGYC	1 cut(s)	1103
BsaJI	CCNNGG	6 cut(s)	712, 773, 1019, 1194, 1446, 1694
BsaXI	ACNNNNNCTCC	6 cut(s)	708, 738, 873, 903, 1491, 1521
Bsc4I	CCNNNNNNNGG	4 cut(s)	496, 718, 995, 1054
Bse1I	ACTGG	4 cut(s)	235, 457, 1525, 1618
Bse3DI	GCAATG	8 cut(s)	1289, 1311, 1317, 1580, 1743, 1810, 1876, 1894
Bse8I	GATNNNNATC	1 cut(s)	33
BseBI	CCWGG	5 cut(s)	83, 288, 1402, 1521, 1695
BseCI	ATCGAT	1 cut(s)	1186
BseDI	CCNNGG	6 cut(s)	712, 773, 1019, 1194, 1446, 1694
BseGI	GGATG	6 cut(s)	515, 585, 615, 1127, 1347, 1810
BseJI	GATNNNNATC	1 cut(s)	33
BseLI	CCNNNNNNNGG	4 cut(s)	496, 718, 995, 1054
BseMI	GCAATG	8 cut(s)	1289, 1311, 1317, 1580, 1743, 1810, 1876, 1894
BseMII	CTCAG	2 cut(s)	558, 1044
BseNI	ACTGG	4 cut(s)	235, 457, 1525, 1618
BseRI	GAGGAG	5 cut(s)	195, 207, 210, 986, 1411
BseSI	GKGCMC	1 cut(s)	906
BseYI	CCCAGC	1 cut(s)	1834
BsgI	GTGCAG	3 cut(s)	296, 1257, 1389
Bsh1236I	CGCG	2 cut(s)	587, 1339
Bsh1285I	CGRYCG	1 cut(s)	1097
BshNI	GGYRCC	2 cut(s)	769, 1102
BshVI	ATCGAT	1 cut(s)	1186
BsiEI	CGRYCG	1 cut(s)	1097
BsiHKAI	GWGCWC	2 cut(s)	573, 1093
BsiHKCI	CYCGRG	2 cut(s)	1074, 1351
BsiSI	CCGG	2 cut(s)	980, 1298
BslFI	GGGAC	1 cut(s)	350
BslI	CCNNNNNNNGG	4 cut(s)	496, 718, 995, 1054
BsmAI	GTCTC	1 cut(s)	391
BsmBI	CGTCTC	1 cut(s)	391
BsmFI	GGGAC	1 cut(s)	350
BsmI	GAATGC	1 cut(s)	826
BsoBI	CYCGRG	2 cut(s)	1074, 1351
Bsp119I	TTCGAA	1 cut(s)	1216
Bsp1286I	GDGCHC	5 cut(s)	573, 906, 980, 1093, 1712
Bsp1720I	GCTNAGC	1 cut(s)	567
Bsp19I	CCATGG	1 cut(s)	1194
BspCNI	CTCAG	2 cut(s)	559, 1045
BspDI	ATCGAT	1 cut(s)	1186
BspFNI	CGCG	2 cut(s)	587, 1339
BspHI	TCATGA	1 cut(s)	934
BspLI	GGNNCC	4 cut(s)	771, 1005, 1104, 1452
BspMAI	CTGCAG	2 cut(s)	901, 1771
BspMI	ACCTGC	1 cut(s)	1658
BspPI	GGATC	7 cut(s)	29, 795, 880, 929, 990, 1728, 1748
BspT104I	TTCGAA	1 cut(s)	1216
BspT107I	GGYRCC	2 cut(s)	769, 1102
BsrBI	CCGCTC	2 cut(s)	201, 334
BsrDI	GCAATG	8 cut(s)	1289, 1311, 1317, 1580, 1743, 1810, 1876, 1894
BsrI	ACTGG	4 cut(s)	235, 457, 1525, 1618
BssECI	CCNNGG	6 cut(s)	712, 773, 1019, 1194, 1446, 1694
BssNI	GRCGYC	1 cut(s)	1103
BssSI	CACGAG	1 cut(s)	1173
BssT1I	CCWWGG	1 cut(s)	1194
Bst2BI	CACGAG	1 cut(s)	1173
Bst2UI	CCWGG	5 cut(s)	83, 288, 1402, 1521, 1695
Bst4CI	ACNGT	5 cut(s)	695, 834, 867, 1465, 1661
BstACI	GRCGYC	1 cut(s)	1103
BstBI	TTCGAA	1 cut(s)	1216
BstC8I	GCNNGC	2 cut(s)	552, 1876
BstDEI	CTNAG	3 cut(s)	567, 1053, 1910
BstDSI	CCRYGG	2 cut(s)	712, 1194
BstEII	GGTNACC	1 cut(s)	622
BstF5I	GGATG	6 cut(s)	515, 585, 615, 1127, 1347, 1810
BstFNI	CGCG	2 cut(s)	587, 1339
BstH2I	RGCGCY	3 cut(s)	702, 1106, 1610
BstHHI	GCGC	4 cut(s)	701, 1105, 1339, 1609
BstMAI	GTCTC	1 cut(s)	391
BstMCI	CGRYCG	1 cut(s)	1097
BstNI	CCWGG	5 cut(s)	83, 288, 1402, 1521, 1695
BstPI	GGTNACC	1 cut(s)	622
BstSCI	CCNGG	6 cut(s)	81, 286, 978, 1400, 1519, 1693
BstSFI	CTRYAG	4 cut(s)	63, 426, 897, 1767
BstSLI	GKGCMC	1 cut(s)	906
BstUI	CGCG	2 cut(s)	587, 1339
BstV2I	GAAGAC	2 cut(s)	93, 828
BstX2I	RGATCY	3 cut(s)	34, 787, 1720
BstYI	RGATCY	3 cut(s)	34, 787, 1720
Bsu15I	ATCGAT	1 cut(s)	1186
BsuTUI	ATCGAT	1 cut(s)	1186
BtgI	CCRYGG	2 cut(s)	712, 1194
BtrI	CACGTC	1 cut(s)	274
BtsCI	GGATG	6 cut(s)	515, 585, 615, 1127, 1347, 1810
BtsI	GCAGTG	2 cut(s)	729, 1764
BtsIMutI	CAGTG	4 cut(s)	501, 729, 1113, 1764
BveI	ACCTGC	1 cut(s)	1658
Cac8I	GCNNGC	2 cut(s)	552, 1876
CaiI	CAGNNNCTG	1 cut(s)	398
CciI	TCATGA	1 cut(s)	934
CfoI	GCGC	4 cut(s)	701, 1105, 1339, 1609
Cfr13I	GGNCC	3 cut(s)	1004, 1450, 1571
ClaI	ATCGAT	1 cut(s)	1186
CseI	GACGC	1 cut(s)	824
Csp6I	GTAC	1 cut(s)	1226
CspCI	CAANNNNNGTGG	1 cut(s)	30
CviAII	CATG	9 cut(s)	166, 172, 419, 433, 784, 935, 1195, 1416, 1429
CviQI	GTAC	1 cut(s)	1226
DdeI	CTNAG	3 cut(s)	567, 1053, 1910
DinI	GGCGCC	1 cut(s)	1104
DraIII	CACNNNGTG	1 cut(s)	718
EaeI	YGGCCR	2 cut(s)	3, 1431
EciI	GGCGGA	2 cut(s)	198, 1733
Ecl136II	GAGCTC	2 cut(s)	571, 1091
Eco130I	CCWWGG	1 cut(s)	1194
Eco24I	GRGCYC	4 cut(s)	573, 980, 1093, 1712
Eco47III	AGCGCT	1 cut(s)	700
Eco53kI	GAGCTC	2 cut(s)	571, 1091
Eco57I	CTGAAG	1 cut(s)	629
Eco88I	CYCGRG	2 cut(s)	1074, 1351
Eco91I	GGTNACC	1 cut(s)	622
EcoICRI	GAGCTC	2 cut(s)	571, 1091
EcoO109I	RGGNCCY	1 cut(s)	1004
EcoO65I	GGTNACC	1 cut(s)	622
EcoRI	GAATTC	1 cut(s)	437
EcoRII	CCWGG	5 cut(s)	81, 286, 1400, 1519, 1693
EcoT14I	CCWWGG	1 cut(s)	1194
EcoT38I	GRGCYC	4 cut(s)	573, 980, 1093, 1712
EgeI	GGCGCC	1 cut(s)	1104
EheI	GGCGCC	1 cut(s)	1104
ErhI	CCWWGG	1 cut(s)	1194
Esp3I	CGTCTC	1 cut(s)	391
FaeI	CATG	9 cut(s)	169, 175, 422, 436, 787, 938, 1198, 1419, 1432
FaqI	GGGAC	1 cut(s)	350
FatI	CATG	9 cut(s)	165, 171, 418, 432, 783, 934, 1194, 1415, 1428
FauI	CCCGC	1 cut(s)	327
FauNDI	CATATG	1 cut(s)	1267
FbaI	TGATCA	4 cut(s)	367, 685, 931, 1110
FokI	GGATG	6 cut(s)	502, 592, 622, 1134, 1354, 1797
FriOI	GRGCYC	4 cut(s)	573, 980, 1093, 1712
FspBI	CTAG	4 cut(s)	480, 764, 1775, 1920
GlaI	GCGC	4 cut(s)	700, 1104, 1338, 1608
GsaI	CCCAGC	1 cut(s)	1838
GsuI	CTGGAG	1 cut(s)	1408
HaeII	RGCGCY	3 cut(s)	702, 1106, 1610
HapII	CCGG	2 cut(s)	980, 1298
HgaI	GACGC	1 cut(s)	824
HhaI	GCGC	4 cut(s)	701, 1105, 1339, 1609
Hin1I	GRCGYC	1 cut(s)	1103
Hin1II	CATG	9 cut(s)	169, 175, 422, 436, 787, 938, 1198, 1419, 1432
Hin6I	GCGC	4 cut(s)	699, 1103, 1337, 1607
HinP1I	GCGC	4 cut(s)	699, 1103, 1337, 1607
HincII	GTYRAC	2 cut(s)	1045, 1673
HindII	GTYRAC	2 cut(s)	1045, 1673
HinfI	GANTC	5 cut(s)	18, 29, 460, 961, 1385
HpaII	CCGG	2 cut(s)	980, 1298
HphI	GGTGA	9 cut(s)	185, 285, 634, 824, 872, 1390, 1479, 1555, 1780
Hpy166II	GTNNAC	5 cut(s)	17, 502, 863, 1045, 1673
Hpy188I	TCNGA	7 cut(s)	34, 690, 844, 966, 1020, 1099, 1557
Hpy188III	TCNNGA	4 cut(s)	371, 870, 935, 952
Hpy8I	GTNNAC	5 cut(s)	17, 502, 863, 1045, 1673
Hpy99I	CGWCG	6 cut(s)	269, 272, 275, 332, 914, 1826
HpyCH4III	ACNGT	5 cut(s)	695, 834, 867, 1465, 1661
HpyCH4IV	ACGT	3 cut(s)	273, 444, 1824
HpyF3I	CTNAG	3 cut(s)	567, 1053, 1910
HpySE526I	ACGT	3 cut(s)	273, 444, 1824
Hsp92I	GRCGYC	1 cut(s)	1103
Hsp92II	CATG	9 cut(s)	169, 175, 422, 436, 787, 938, 1198, 1419, 1432
HspAI	GCGC	4 cut(s)	699, 1103, 1337, 1607
KasI	GGCGCC	1 cut(s)	1102
Ksp22I	TGATCA	4 cut(s)	367, 685, 931, 1110
LmnI	GCTCC	3 cut(s)	263, 1424, 1512
LweI	GCATC	5 cut(s)	600, 657, 1178, 1325, 1332
MaeI	CTAG	4 cut(s)	480, 764, 1775, 1920
MaeII	ACGT	3 cut(s)	273, 444, 1824
MaeIII	GTNAC	6 cut(s)	485, 622, 812, 990, 1465, 1644
MbiI	CCGCTC	2 cut(s)	201, 334
MboII	GAAGA	7 cut(s)	98, 334, 455, 551, 740, 748, 828
MfeI	CAATTG	1 cut(s)	1894
MflI	RGATCY	3 cut(s)	34, 787, 1720
MhlI	GDGCHC	5 cut(s)	573, 906, 980, 1093, 1712
MlsI	TGGCCA	2 cut(s)	5, 1433
MluCI	AATT	9 cut(s)	437, 631, 661, 1027, 1558, 1593, 1625, 1894, 1933
MluNI	TGGCCA	2 cut(s)	5, 1433
Mly113I	GGCGCC	1 cut(s)	1103
MlyI	GAGTC	2 cut(s)	12, 1379
MmeI	TCCRAC	2 cut(s)	330, 543
Mox20I	TGGCCA	2 cut(s)	5, 1433
MroXI	GAANNNNTTC	1 cut(s)	947
MscI	TGGCCA	2 cut(s)	5, 1433
MseI	TTAA	3 cut(s)	615, 1478, 1936
MslI	CAYNNNNRTG	1 cut(s)	170
Msp20I	TGGCCA	2 cut(s)	5, 1433
MspA1I	CMGCKG	3 cut(s)	1739, 1751, 1781
MspI	CCGG	2 cut(s)	980, 1298
MspR9I	CCNGG	6 cut(s)	83, 288, 980, 1402, 1521, 1695
MunI	CAATTG	1 cut(s)	1894
Mva1269I	GAATGC	1 cut(s)	826
MvaI	CCWGG	5 cut(s)	83, 288, 1402, 1521, 1695
MvnI	CGCG	2 cut(s)	587, 1339
NarI	GGCGCC	1 cut(s)	1103
NciI	CCSGG	1 cut(s)	980
NcoI	CCATGG	1 cut(s)	1194
NdeI	CATATG	1 cut(s)	1267
NlaIII	CATG	9 cut(s)	169, 175, 422, 436, 787, 938, 1198, 1419, 1432
NlaIV	GGNNCC	4 cut(s)	771, 1005, 1104, 1452
NmeAIII	GCCGAG	2 cut(s)	798, 1427
NmuCI	GTSAC	5 cut(s)	485, 622, 812, 990, 1644
NspV	TTCGAA	1 cut(s)	1216
PagI	TCATGA	1 cut(s)	934
PcsI	WCGNNNNNNNCGW	1 cut(s)	270
PctI	GAATGC	1 cut(s)	826
PdmI	GAANNNNTTC	1 cut(s)	947
PfeI	GAWTC	3 cut(s)	29, 460, 961
PflMI	CCANNNNNTGG	2 cut(s)	496, 718
PfoI	TCCNGGA	1 cut(s)	81
Ple19I	CGATCG	1 cut(s)	1097
PleI	GAGTC	2 cut(s)	12, 1379
PluTI	GGCGCC	1 cut(s)	1106
PpsI	GAGTC	2 cut(s)	12, 1379
Psp124BI	GAGCTC	2 cut(s)	573, 1093
Psp6I	CCWGG	5 cut(s)	81, 286, 1400, 1519, 1693
PspEI	GGTNACC	1 cut(s)	622
PspFI	CCCAGC	1 cut(s)	1834
PspGI	CCWGG	5 cut(s)	81, 286, 1400, 1519, 1693
PspN4I	GGNNCC	4 cut(s)	771, 1005, 1104, 1452
PspPI	GGNCC	3 cut(s)	1004, 1450, 1571
PstI	CTGCAG	2 cut(s)	901, 1771
PstNI	CAGNNNCTG	1 cut(s)	398
PsuI	RGATCY	3 cut(s)	34, 787, 1720
PvuI	CGATCG	1 cut(s)	1097
PvuII	CAGCTG	2 cut(s)	1751, 1781
RsaI	GTAC	1 cut(s)	1227
RsaNI	GTAC	1 cut(s)	1226
RseI	CAYNNNNRTG	1 cut(s)	170
SacI	GAGCTC	2 cut(s)	573, 1093
SaqAI	TTAA	3 cut(s)	615, 1478, 1936
Sau96I	GGNCC	3 cut(s)	1004, 1450, 1571
SchI	GAGTC	2 cut(s)	12, 1379
ScrFI	CCNGG	6 cut(s)	83, 288, 980, 1402, 1521, 1695
SduI	GDGCHC	5 cut(s)	573, 906, 980, 1093, 1712
SfaNI	GCATC	5 cut(s)	600, 657, 1178, 1325, 1332
SfcI	CTRYAG	4 cut(s)	63, 426, 897, 1767
SfoI	GGCGCC	1 cut(s)	1104
SfuI	TTCGAA	1 cut(s)	1216
SmiMI	CAYNNNNRTG	1 cut(s)	170
SmlI	CTYRAG	1 cut(s)	572
SmoI	CTYRAG	1 cut(s)	572
SpeI	ACTAGT	2 cut(s)	479, 763
Sse9I	AATT	9 cut(s)	437, 631, 661, 1027, 1558, 1593, 1625, 1894, 1933
SspDI	GGCGCC	1 cut(s)	1102
SspMI	CTAG	4 cut(s)	480, 764, 1775, 1920
SstI	GAGCTC	2 cut(s)	573, 1093
StyD4I	CCNGG	6 cut(s)	81, 286, 978, 1400, 1519, 1693
StyI	CCWWGG	1 cut(s)	1194
TaaI	ACNGT	5 cut(s)	695, 834, 867, 1465, 1661
TaiI	ACGT	3 cut(s)	276, 447, 1827
TaqI	TCGA	6 cut(s)	327, 951, 1093, 1186, 1216, 1319
TaqII	GACCGA	1 cut(s)	105
TasI	AATT	9 cut(s)	437, 631, 661, 1027, 1558, 1593, 1625, 1894, 1933
TatI	WGTACW	1 cut(s)	1225
TauI	GCSGC	3 cut(s)	201, 1667, 1733
TfiI	GAWTC	3 cut(s)	29, 460, 961
Tru1I	TTAA	3 cut(s)	615, 1478, 1936
Tru9I	TTAA	3 cut(s)	615, 1478, 1936
TscAI	CASTG	4 cut(s)	501, 729, 1120, 1771
TseFI	GTSAC	5 cut(s)	485, 622, 812, 990, 1644
Tsp45I	GTSAC	5 cut(s)	485, 622, 812, 990, 1644
TspDTI	ATGAA	7 cut(s)	504, 596, 758, 772, 861, 951, 1284
TspGWI	ACGGA	3 cut(s)	729, 1155, 1527
TspRI	CASTG	4 cut(s)	501, 729, 1120, 1771
Van91I	CCANNNNNTGG	2 cut(s)	496, 718
XapI	RAATTY	5 cut(s)	437, 661, 1558, 1593, 1625
XmnI	GAANNNNTTC	1 cut(s)	947
XspI	CTAG	4 cut(s)	480, 764, 1775, 1920

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.