Rh3AG270200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002724
Physical Location & Seq
Forward (+)
30754345 .. 30756814
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG270200.1

Sequence Viewer

Length: 1329 bp
ATGGATGAACAACGCTGTGACTTGAAATACTTGCACTCGGACCAGAGCTTTCTGGGGCTCAGTACTAGTTCCGATCATCGTCTAGTTGAACCCCCAATAAAAGAAGAAACATTATTCAAAGAGATCAAAGAAGTTGATTTCTTTTCCAATAATAGACTTCCCGATCAACCGGCGCCTGGTCATGCCGAGCTTCAAGTTGAGTTACAGCGAGAATACGAAGAGAATCAAAAGCTCAGAACCATGTTGGACCAAATCACCAAAAGCTACAATGGGTTGCAGGCTCAGCTGCGCATGATCAGACTTCAAGAGCAGCTGCAAATTTGTGCAGAAGACAGCATTAATCTTGATCCGAAGTTATCCTTCCTACATGATGATCAATACCCCAAAAGTAGTTCTCATCACGATCATGATCAAGAACAAGTTCTTGCTGCTACTGATCATGATCAGGTTCCTTCAAGAAGGGCTAGGGTCTCCGTACGAGCAAGATCGGAAGCTCCCATGATTAGAGATGGATGTCAATGGAGGAAATACGGTCAAAAGATGGCCAAGGGTAATCCTTGTCCCCGTGCTTACTATCGCTGCACTATGTTCGCTGGATGCCCCGTTCGGAAACAGGTGCAAAGAATGGCAGAGGACAAGACCATTCTGATAACAACGTATGAAGGTAATCACAACCACCCTCTTCCTGCAGCTGCGATATCTATGGCTAATACCACATCAGCAGCAGCAGCCATGTTGATATCGGGTTCAACAACCAGCAAGGAAGCTCATCATCATCTAACACACCCTACAGGCTTGTTCTCTTCTCTTCAGCCGCTCTATGGTTCATCCATGGCCACCTTTTCAACCTCTGCACCATTTCCCACAATCACACTGGACATGACCCGCACCCCTAACCCTAACCCTAATCCCATGCAGTTCATGGACAGTAGTGCCACATTTCCTAATCCTTTGCAGGGTAATAGTCATCCGCTTATAGGGCACCCCATGTGTTTGAATTTCCCTCAGCATAATCATGCAGCGCTAACACCAGCGGCAATGCAACTAGCAGAACAACCCCACGGTTCGATGGTTGAGACAATAACTGCAGCCATTACTTCTGACCCGAAGTTCACTACAGCCCTAGCTGCTGCCATCTCAAGTAGAATGAATAATGTTGAGAATATTAATGCTGGATTACATGGAAATACCAATGGTATTAGTTCCCTTAACAGTAATGGGAATGGGGTTGCGCAGCATTATCTGGCTCCCCACAGCTTCCTCAATCCTGTACCACTTTCTCCACCACCTAATCCTGCATGCATGATGCATGGGGTATATATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

442

Amino Acids

48.93

Weight (kDa)

6.44

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 170 - 227 2.4e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 290, 1233
AccB1I GGYRCC 2 cut(s) 172, 981
AccBSI CCGCTC 1 cut(s) 817
AciI CCGC 4 cut(s) 815, 886, 971, 1034
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 2 cut(s) 543, 834
AcsI RAATTY 2 cut(s) 318, 997
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 3 cut(s) 64, 477, 1272
AfeI AGCGCT 1 cut(s) 1023
AfiI CCNNNNNNNGG 4 cut(s) 176, 821, 956, 977
AgsI TTSAA 9 cut(s) 25, 89, 118, 194, 305, 456, 750, 846, 997
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 588, 620
Alw26I GTCTC 2 cut(s) 475, 1070
AlwI GGATC 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 1023
AoxI GGCC 2 cut(s) 543, 834
ApoI RAATTY 2 cut(s) 318, 997
AseI ATTAAT 2 cut(s) 339, 1167
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 4 cut(s) 175, 291, 1024, 1234
AspS9I GGNCC 2 cut(s) 40, 247
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 2 cut(s) 40, 247
BaeGI GKGCMC 1 cut(s) 984
BalI TGGCCA 2 cut(s) 545, 836
BanI GGYRCC 2 cut(s) 172, 981
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 1005
BccI CCATC 4 cut(s) 503, 535, 1063, 1142
BcgI CGANNNNNNTGC 2 cut(s) 571, 605
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 5 cut(s) 294, 373, 409, 436, 442
BcoDI GTCTC 2 cut(s) 475, 1070
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 5 cut(s) 66, 83, 465, 1046, 1124
BfmI CTRYAG 4 cut(s) 687, 789, 1086, 1116
BfoI RGCGCY 2 cut(s) 176, 1025
BlpI GCTNAGC 1 cut(s) 282
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 40, 247
BmgT120I GGNCC 2 cut(s) 40, 247
BmiI GGNNCC 4 cut(s) 174, 450, 983, 1248
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 587, 1296
BpiI GAAGAC 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 1123
BsaBI GATNNNNATC 2 cut(s) 408, 441
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 3 cut(s) 546, 831, 1060
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 821, 956, 977
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 879
Bse3DI GCAATG 1 cut(s) 1044
Bse8I GATNNNNATC 2 cut(s) 408, 441
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 546, 831, 1060
BseGI GGATG 5 cut(s) 10, 518, 602, 827, 967
BseJI GATNNNNATC 2 cut(s) 408, 441
BseLI CCNNNNNNNGG 4 cut(s) 176, 821, 956, 977
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 4 cut(s) 73, 247, 296, 1019
BseNI ACTGG 1 cut(s) 879
BseSI GKGCMC 1 cut(s) 984
BsgI GTGCAG 3 cut(s) 345, 565, 837
BshFI GGCC 2 cut(s) 545, 836
BshNI GGYRCC 2 cut(s) 172, 981
BsiSI CCGG 1 cut(s) 170
BsiWI CGTACG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 4 cut(s) 176, 821, 956, 977
BsmAI GTCTC 2 cut(s) 475, 1070
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 2 cut(s) 545, 836
Bso31I GGTCTC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 60, 984
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 4 cut(s) 815, 886, 971, 1034
BspANI GGCC 2 cut(s) 545, 836
BspCNI CTCAG 4 cut(s) 72, 246, 295, 1018
BspHI TCATGA 2 cut(s) 406, 439
BspLI GGNNCC 4 cut(s) 174, 450, 983, 1248
BspMAI CTGCAG 2 cut(s) 691, 1090
BspPI GGATC 1 cut(s) 341
BspT107I GGYRCC 2 cut(s) 172, 981
BspTNI GGTCTC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 817
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 879
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 546, 831, 1060
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 546, 831
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 4 cut(s) 533, 929, 1064, 1214
Bst6I CTCTTC 4 cut(s) 213, 687, 808, 813
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 279, 1300
BstDEI CTNAG 4 cut(s) 59, 233, 282, 1005
BstDSI CCRYGG 2 cut(s) 831, 1060
BstF5I GGATG 5 cut(s) 10, 518, 602, 827, 967
BstH2I RGCGCY 2 cut(s) 176, 1025
BstHHI GCGC 4 cut(s) 175, 291, 1024, 1234
BstMAI GTCTC 2 cut(s) 475, 1070
BstMWI GCNNNNNNNGC 4 cut(s) 283, 728, 979, 1127
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 1302
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 4 cut(s) 687, 789, 1086, 1116
BstSLI GKGCMC 1 cut(s) 984
BstV2I GAAGAC 1 cut(s) 336
BsuRI GGCC 2 cut(s) 545, 836
BtgI CCRYGG 2 cut(s) 831, 1060
BtsCI GGATG 5 cut(s) 10, 518, 602, 827, 967
BtsIMutI CAGTG 1 cut(s) 872
Cac8I GCNNGC 2 cut(s) 279, 1300
CciI TCATGA 2 cut(s) 406, 439
CfoI GCGC 4 cut(s) 175, 291, 1024, 1234
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 40, 247
Csp6I GTAC 3 cut(s) 63, 476, 1271
CviQI GTAC 3 cut(s) 63, 476, 1271
DdeI CTNAG 4 cut(s) 59, 233, 282, 1005
DinI GGCGCC 1 cut(s) 174
EaeI YGGCCR 2 cut(s) 543, 834
Eam1104I CTCTTC 4 cut(s) 213, 687, 808, 813
EarI CTCTTC 4 cut(s) 213, 687, 808, 813
Eco130I CCWWGG 2 cut(s) 546, 831
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 2 cut(s) 699, 741
Eco47I GGWCC 2 cut(s) 40, 247
Eco47III AGCGCT 1 cut(s) 1023
Eco57I CTGAAG 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 2 cut(s) 699, 741
EcoT14I CCWWGG 2 cut(s) 546, 831
EcoT22I ATGCAT 2 cut(s) 1304, 1311
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 546, 831
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 546
FauI CCCGC 1 cut(s) 893
FbaI TGATCA 5 cut(s) 294, 373, 409, 436, 442
FokI GGATG 5 cut(s) 17, 525, 609, 814, 954
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 5 cut(s) 66, 83, 465, 1046, 1124
FspI TGCGCA 2 cut(s) 290, 1233
GlaI GCGC 4 cut(s) 174, 290, 1023, 1233
HaeII RGCGCY 2 cut(s) 176, 1025
HaeIII GGCC 2 cut(s) 545, 836
HapII CCGG 1 cut(s) 170
HhaI GCGC 4 cut(s) 175, 291, 1024, 1234
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 4 cut(s) 173, 289, 1022, 1232
HinP1I GCGC 4 cut(s) 173, 289, 1022, 1232
HinfI GANTC 1 cut(s) 223
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 1113
Hpy188I TCNGA 9 cut(s) 40, 73, 236, 299, 351, 490, 609, 648, 1102
Hpy188III TCNNGA 8 cut(s) 161, 305, 344, 401, 407, 413, 440, 456
Hpy8I GTNNAC 1 cut(s) 1113
HpyAV CCTTC 4 cut(s) 370, 453, 462, 656
HpyCH4III ACNGT 4 cut(s) 533, 929, 1064, 1214
HpyCH4IV ACGT 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 728, 979, 1127
HpyF3I CTNAG 4 cut(s) 59, 233, 282, 1005
HpySE526I ACGT 1 cut(s) 656
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 4 cut(s) 173, 289, 1022, 1232
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 5 cut(s) 294, 373, 409, 436, 442
LmnI GCTCC 2 cut(s) 499, 1252
LweI GCATC 2 cut(s) 587, 1296
MaeI CTAG 5 cut(s) 66, 83, 465, 1046, 1124
MaeII ACGT 1 cut(s) 656
MaeIII GTNAC 2 cut(s) 17, 201
MbiI CCGCTC 1 cut(s) 817
MboII GAAGA 6 cut(s) 116, 230, 341, 674, 795, 800
MhlI GDGCHC 2 cut(s) 60, 984
MlsI TGGCCA 2 cut(s) 545, 836
MluCI AATT 2 cut(s) 318, 997
MluNI TGGCCA 2 cut(s) 545, 836
Mly113I GGCGCC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 6 cut(s) 516, 625, 690, 859, 1014, 1271
Mox20I TGGCCA 2 cut(s) 545, 836
Mph1103I ATGCAT 2 cut(s) 1304, 1311
MroXI GAANNNNTTC 1 cut(s) 420
MscI TGGCCA 2 cut(s) 545, 836
MseI TTAA 3 cut(s) 339, 1167, 1209
MslI CAYNNNNRTG 2 cut(s) 405, 1014
Msp20I TGGCCA 2 cut(s) 545, 836
MspA1I CMGCKG 4 cut(s) 286, 313, 692, 1034
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 283, 728, 979, 1127
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 831
NlaIV GGNNCC 4 cut(s) 174, 450, 983, 1248
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 17
NsbI TGCGCA 2 cut(s) 290, 1233
NsiI ATGCAT 2 cut(s) 1304, 1311
NspI RCATGY 1 cut(s) 1302
PaeI GCATGC 1 cut(s) 1302
PagI TCATGA 2 cut(s) 406, 439
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 223
Pfl23II CGTACG 1 cut(s) 475
PluTI GGCGCC 1 cut(s) 176
PshBI ATTAAT 2 cut(s) 339, 1167
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspLI CGTACG 1 cut(s) 475
PspN4I GGNNCC 4 cut(s) 174, 450, 983, 1248
PspPI GGNCC 2 cut(s) 40, 247
PstI CTGCAG 2 cut(s) 691, 1090
PvuII CAGCTG 3 cut(s) 286, 313, 692
RsaI GTAC 3 cut(s) 64, 477, 1272
RsaNI GTAC 3 cut(s) 63, 476, 1271
RseI CAYNNNNRTG 2 cut(s) 405, 1014
SaqAI TTAA 3 cut(s) 339, 1167, 1209
Sau96I GGNCC 2 cut(s) 40, 247
ScaI AGTACT 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 60, 984
SfaNI GCATC 2 cut(s) 587, 1296
SfcI CTRYAG 4 cut(s) 687, 789, 1086, 1116
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 40, 247
SmiMI CAYNNNNRTG 2 cut(s) 405, 1014
SmlI CTYRAG 1 cut(s) 1138
SmoI CTYRAG 1 cut(s) 1138
SpeI ACTAGT 1 cut(s) 65
SphI GCATGC 1 cut(s) 1302
Sse9I AATT 2 cut(s) 318, 997
SsiI CCGC 4 cut(s) 815, 886, 971, 1034
SspDI GGCGCC 1 cut(s) 172
SspI AATATT 1 cut(s) 1165
SspMI CTAG 5 cut(s) 66, 83, 465, 1046, 1124
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 2 cut(s) 546, 831
TaaI ACNGT 4 cut(s) 533, 929, 1064, 1214
TaiI ACGT 1 cut(s) 659
TaqI TCGA 1 cut(s) 1067
TasI AATT 2 cut(s) 318, 997
TatI WGTACW 1 cut(s) 62
TauI GCSGC 2 cut(s) 817, 1037
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 3 cut(s) 339, 1167, 1209
Tru9I TTAA 3 cut(s) 339, 1167, 1209
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 21, 675, 816, 910, 1163
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 40, 247
VspI ATTAAT 2 cut(s) 339, 1167
XapI RAATTY 2 cut(s) 318, 997
XceI RCATGY 1 cut(s) 1302
XcmI CCANNNNNNNNNTGG 2 cut(s) 871, 919
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 5 cut(s) 66, 83, 465, 1046, 1124
ZrmI AGTACT 1 cut(s) 64
Zsp2I ATGCAT 2 cut(s) 1304, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.