AT4G05010

F-box family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002768
Physical Location & Seq
Forward (+)
2567183 .. 2568437
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05010.1

Sequence Viewer

Length: 495 bp
ATGGCGTATTTGAGTGATGGATTTGAAATGAAGTCGACTCGTGAGTATGAGAGATTAGGGTTAGGGTTTGTGAGGTTTACTAGAGGATTAGGGAGGAAAAGGATTCTCATATCTAAACGAGCTCCGGAAAATGATTCGCCGCCGGTGAAGAGACCAAGTCATGAAACGACGGAATCTTGTAGGTCTCTGCTTGAAACTCTGCATCAAGATATTTTGATTCGAGTACTTTGTCATGTGGATCACGAGGACCTAGCCACACTAAAACGTGTATCTAAAACAATAAGAAAAGCTGTAATTGAAGCAAAGAAGTCGCATTTTGATTATAGCACACCTAAAAAGAGATTGCCATTTCGAGACGCAATACTAATCCTTGATTCAAATTCGAATTCCAGTAGTCAAGATGATGAAATGGAGCCTCCTAATGCACCAATTCGAAGGAGGTTTATTAATCGAGAATCGGATTTGTCGAAGATCTCTATGGTATTGTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

164

Amino Acids

18.97

Weight (kDa)

9.69

Isoelectric Point (pI)

66.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 124
AciI CCGC 1 cut(s) 140
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 2 cut(s) 379, 385
AfaI GTAC 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 4 cut(s) 26, 194, 299, 378
AluBI AGCT 2 cut(s) 122, 290
AluI AGCT 2 cut(s) 122, 290
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 3 cut(s) 145, 189, 348
AlwI GGATC 1 cut(s) 246
Aor13HI TCCGGA 1 cut(s) 124
ApoI RAATTY 2 cut(s) 379, 385
AseI ATTAAT 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 157
AsuII TTCGAA 2 cut(s) 383, 433
AvaII GGWCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 124
BauI CACGAG 2 cut(s) 39, 242
Bbv12I GWGCWC 1 cut(s) 124
BccI CCATC 1 cut(s) 11
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BcoDI GTCTC 3 cut(s) 145, 189, 348
BfaI CTAG 2 cut(s) 81, 251
BglII AGATCT 1 cut(s) 471
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
BmcAI AGTACT 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 1 cut(s) 211
Bpu14I TTCGAA 2 cut(s) 383, 433
BsaI GGTCTC 2 cut(s) 145, 189
BsaWI WCCGGW 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 124
BseNI ACTGG 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 125, 143
BsmAI GTCTC 3 cut(s) 145, 189, 348
BsmBI CGTCTC 1 cut(s) 348
Bso31I GGTCTC 2 cut(s) 145, 189
Bsp119I TTCGAA 2 cut(s) 383, 433
Bsp1286I GDGCHC 1 cut(s) 124
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 2 cut(s) 238, 471
BspACI CCGC 1 cut(s) 140
BspEI TCCGGA 1 cut(s) 124
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 1 cut(s) 246
BspT104I TTCGAA 2 cut(s) 383, 433
BspTNI GGTCTC 2 cut(s) 145, 189
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 390
BssAI RCCGGY 1 cut(s) 142
BssMI GATC 2 cut(s) 238, 471
BssSI CACGAG 2 cut(s) 39, 242
Bst2BI CACGAG 2 cut(s) 39, 242
Bst6I CTCTTC 1 cut(s) 143
BstBI TTCGAA 2 cut(s) 383, 433
BstKTI GATC 2 cut(s) 241, 474
BstMAI GTCTC 3 cut(s) 145, 189, 348
BstMBI GATC 2 cut(s) 238, 471
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
CciI TCATGA 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 161, 233
CviJI RGCY 4 cut(s) 122, 254, 290, 415
CviKI_1 RGCY 4 cut(s) 122, 254, 290, 415
CviQI GTAC 1 cut(s) 224
DpnI GATC 2 cut(s) 240, 473
DpnII GATC 2 cut(s) 238, 471
DraI TTTAAA 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 122
Eco24I GRGCYC 1 cut(s) 124
Eco31I GGTCTC 2 cut(s) 145, 189
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 124
Esp3I CGTCTC 1 cut(s) 348
FaeI CATG 2 cut(s) 164, 236
FaiI YATR 6 cut(s) 48, 110, 162, 234, 324, 479
FatI CATG 2 cut(s) 160, 232
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 1 cut(s) 140
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 81, 251
GluI GCNGC 1 cut(s) 140
HapII CCGG 2 cut(s) 125, 143
HgaI GACGC 1 cut(s) 365
Hin1II CATG 2 cut(s) 164, 236
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 7 cut(s) 37, 103, 134, 173, 217, 374, 455
HpaII CCGG 2 cut(s) 125, 143
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 36, 78
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 8 cut(s) 41, 125, 161, 206, 242, 353, 398, 452
Hpy8I GTNNAC 2 cut(s) 36, 78
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 1 cut(s) 429
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 2 cut(s) 202, 425
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 2 cut(s) 164, 236
Kpn2I TCCGGA 1 cut(s) 124
Kzo9I GATC 2 cut(s) 238, 471
LmnI GCTCC 2 cut(s) 127, 412
LpnPI CCDG 3 cut(s) 138, 156, 403
LweI GCATC 1 cut(s) 211
MaeI CTAG 2 cut(s) 81, 251
MaeII ACGT 1 cut(s) 265
MalI GATC 2 cut(s) 240, 473
MboI GATC 2 cut(s) 238, 471
MboII GAAGA 2 cut(s) 160, 481
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 4 cut(s) 294, 379, 385, 429
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 6 cut(s) 66, 77, 87, 238, 426, 432
MroI TCCGGA 1 cut(s) 124
MseI TTAA 2 cut(s) 447, 489
MspI CCGG 2 cut(s) 125, 143
NdeII GATC 2 cut(s) 238, 471
NlaIII CATG 2 cut(s) 164, 236
NlaIV GGNNCC 1 cut(s) 414
NspV TTCGAA 2 cut(s) 383, 433
PagI TCATGA 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 464
PfeI GAWTC 6 cut(s) 103, 134, 173, 217, 374, 455
PflFI GACNNNGTC 1 cut(s) 156
PkrI GCNGC 1 cut(s) 141
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 247
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 1 cut(s) 471
PsyI GACNNNGTC 1 cut(s) 156
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SacI GAGCTC 1 cut(s) 124
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 2 cut(s) 447, 489
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 2 cut(s) 238, 471
Sau96I GGNCC 1 cut(s) 247
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 31
SduI GDGCHC 1 cut(s) 124
SetI ASST 8 cut(s) 77, 124, 185, 252, 268, 292, 334, 443
SfaNI GCATC 1 cut(s) 211
SfuI TTCGAA 2 cut(s) 383, 433
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 1 cut(s) 247
Sse9I AATT 4 cut(s) 294, 379, 385, 429
SsiI CCGC 1 cut(s) 140
SspMI CTAG 2 cut(s) 81, 251
SstI GAGCTC 1 cut(s) 124
TaiI ACGT 1 cut(s) 268
TaqI TCGA 7 cut(s) 35, 220, 352, 383, 433, 451, 467
TasI AATT 4 cut(s) 294, 379, 385, 429
TatI WGTACW 1 cut(s) 223
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 6 cut(s) 103, 134, 173, 217, 374, 455
Tru1I TTAA 2 cut(s) 447, 489
Tru9I TTAA 2 cut(s) 447, 489
TspDTI ATGAA 3 cut(s) 44, 177, 420
TspGWI ACGGA 1 cut(s) 185
Tth111I GACNNNGTC 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 2 cut(s) 379, 385
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 2 cut(s) 81, 251
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.