AT4G05400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003835
Physical Location & Seq
Forward (+)
2741124 .. 2742949
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05400.2

Sequence Viewer

Length: 753 bp
ATGATTCGAGTTACGAAACCAAAAGCGATCATCGAATCACTGAGTTACCATCTTATCCAAAGATGCTCCGCAAGTGGAACTCCTAAAGGAAAAGCAAAGCTGAAGACAGGTCAGCCATTGAAGCGTAACAAACTCTCCATCAAGAAAGGTGGTGGCGGCGGCGGAGCACCTGTTGAAGGAGAGGAAGCAGTTAAAGGAAAAGGTAGAATTTCCGATGAGAAGCAGAAACTCTACGAGCAATGCTTAAACGCTCCTTGTCCTGTTCGCTACTTGACCCCCCAAGAGATCGAGCGTGAGGCGCAACGTGAGAAGCTAGGTTTGATTAGTAAAGAGAAGCAACGAGATATGGAGATCCAGAAGAAAGGAGGAGCTGCTTCAATGGGAGTCACCGATGAGCCGATGCGAATTGGGACGCCAGGTTTGGATTACATTTCGTTAGGGATTTTTGAAGCTGAGGAATTGCCTAAGTATAAGCTTACGGAAGAAGATGGAGAGAGACTTGCGAAGGAGTATAGTAAAGTGTTGATGAGAGAGCATAGAGAGCGTCGTGCAGCTGAAACTGCGCTTTTGAATTTGAAGAAATCTGCTATTGAGGCATTGCCGGAGAATCTGAAGAAGGCGGCGTTAGAGCCTGATTTGACACCGTTTCCGGCGAATCGAGGTATGGCTACTCTTACACCGCCTATTGAAGGGTATCTTGAGAAGGTTATGGATGCAGCTAAGAAGAGTTCAAGTAAAGAGAAGTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.63

Weight (kDa)

9.46

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 69, 156, 159, 162, 620, 680
AclWI GGATC 1 cut(s) 346
AcsI RAATTY 2 cut(s) 207, 571
AcuI CTGAAG 2 cut(s) 122, 632
AcyI GRCGYC 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 176, 689
AgsI TTSAA 8 cut(s) 121, 176, 378, 449, 571, 577, 689, 732
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 7 cut(s) 100, 313, 371, 452, 475, 554, 719
AluI AGCT 7 cut(s) 100, 313, 371, 452, 475, 554, 719
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 1 cut(s) 346
ApeKI GCWGC 3 cut(s) 371, 551, 716
ApoI RAATTY 2 cut(s) 207, 571
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AspLEI GCGC 2 cut(s) 301, 565
AsuHPI GGTGA 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 169
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 358, 563, 728
BccI CCATC 3 cut(s) 57, 146, 482
BciT130I CCWGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 1 cut(s) 314
BisI GCNGC 6 cut(s) 157, 160, 372, 552, 621, 717
BlsI GCNGC 6 cut(s) 158, 161, 373, 553, 622, 718
Bme1390I CCNGG 1 cut(s) 417
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 3 cut(s) 53, 390, 703
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 413
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 689
Bse3DI GCAATG 2 cut(s) 245, 596
BseBI CCWGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 718
BseLI CCNNNNNNNGG 2 cut(s) 176, 689
BseMI GCAATG 2 cut(s) 245, 596
BseMII CTCAG 2 cut(s) 32, 444
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 3 cut(s) 358, 563, 728
BsgI GTGCAG 1 cut(s) 570
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 2 cut(s) 602, 650
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 176, 689
BsmAI GTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 3 cut(s) 27, 285, 351
BspACI CCGC 6 cut(s) 69, 156, 159, 162, 620, 680
BspCNI CTCAG 2 cut(s) 33, 445
BspPI GGATC 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 245, 596
BssMI GATC 3 cut(s) 27, 285, 351
BssNI GRCGYC 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 1 cut(s) 645
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 1 cut(s) 413
BstDEI CTNAG 4 cut(s) 41, 453, 465, 720
BstENI CCTNNNNNAGG 2 cut(s) 174, 687
BstF5I GGATG 1 cut(s) 718
BstHHI GCGC 2 cut(s) 301, 565
BstKTI GATC 3 cut(s) 30, 288, 354
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 3 cut(s) 27, 285, 351
BstMWI GCNNNNNNNGC 5 cut(s) 121, 298, 541, 560, 593
BstNI CCWGG 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 415
BstV1I GCAGC 3 cut(s) 358, 563, 728
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 351
BstYI RGATCY 1 cut(s) 351
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 1 cut(s) 38
CfoI GCGC 2 cut(s) 301, 565
CseI GACGC 2 cut(s) 421, 533
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
DdeI CTNAG 4 cut(s) 41, 453, 465, 720
DpnI GATC 3 cut(s) 29, 287, 353
DpnII GATC 3 cut(s) 27, 285, 351
Eam1104I CTCTTC 1 cut(s) 719
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 122, 632
EcoNI CCTNNNNNAGG 2 cut(s) 174, 687
EcoRII CCWGG 1 cut(s) 415
FaiI YATR 6 cut(s) 347, 471, 513, 537, 665, 710
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FaqI GGGAC 1 cut(s) 424
Fnu4HI GCNGC 6 cut(s) 157, 160, 372, 552, 621, 717
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 6 cut(s) 157, 160, 372, 552, 621, 717
FspBI CTAG 1 cut(s) 314
GlaI GCGC 2 cut(s) 300, 564
GluI GCNGC 6 cut(s) 157, 160, 372, 552, 621, 717
HapII CCGG 2 cut(s) 602, 650
HgaI GACGC 2 cut(s) 421, 533
HhaI GCGC 2 cut(s) 301, 565
Hin1I GRCGYC 1 cut(s) 413
Hin6I GCGC 2 cut(s) 299, 563
HinP1I GCGC 2 cut(s) 299, 563
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 5 cut(s) 4, 35, 384, 607, 655
HpaII CCGG 2 cut(s) 602, 650
HphI GGTGA 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 214, 612
Hpy188III TCNNGA 3 cut(s) 142, 355, 698
Hpy99I CGWCG 1 cut(s) 549
HpyAV CCTTC 5 cut(s) 170, 499, 610, 683, 697
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 551, 716
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 298, 541, 560, 593
HpyF3I CTNAG 4 cut(s) 41, 453, 465, 720
HpySE526I ACGT 1 cut(s) 304
Hsp92I GRCGYC 1 cut(s) 413
HspAI GCGC 2 cut(s) 299, 563
Kzo9I GATC 3 cut(s) 27, 285, 351
LmnI GCTCC 4 cut(s) 71, 164, 256, 368
LpnPI CCDG 9 cut(s) 93, 183, 273, 368, 402, 429, 615, 645, 663
Lsp1109I GCAGC 3 cut(s) 358, 563, 728
LweI GCATC 3 cut(s) 53, 390, 703
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 4 cut(s) 10, 44, 125, 385
MalI GATC 3 cut(s) 29, 287, 353
MboI GATC 3 cut(s) 27, 285, 351
MboII GAAGA 7 cut(s) 115, 370, 494, 497, 589, 625, 736
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 4 cut(s) 207, 405, 458, 571
MlyI GAGTC 1 cut(s) 393
MnlI CCTC 6 cut(s) 175, 289, 359, 448, 586, 653
MseI TTAA 2 cut(s) 192, 245
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 2 cut(s) 602, 650
MspR9I CCNGG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 5 cut(s) 121, 298, 541, 560, 593
NdeII GATC 3 cut(s) 27, 285, 351
NmuCI GTSAC 1 cut(s) 385
PfeI GAWTC 4 cut(s) 4, 35, 607, 655
PkrI GCNGC 6 cut(s) 158, 161, 373, 553, 622, 718
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PsuI RGATCY 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 554
SaqAI TTAA 2 cut(s) 192, 245
SatI GCNGC 6 cut(s) 157, 160, 372, 552, 621, 717
Sau3AI GATC 3 cut(s) 27, 285, 351
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 169
SfaNI GCATC 3 cut(s) 53, 390, 703
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
Sse9I AATT 4 cut(s) 207, 405, 458, 571
SsiI CCGC 6 cut(s) 69, 156, 159, 162, 620, 680
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 415
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 1 cut(s) 307
TaqI TCGA 4 cut(s) 7, 33, 288, 658
TasI AATT 4 cut(s) 207, 405, 458, 571
TauI GCSGC 3 cut(s) 159, 162, 623
TfiI GAWTC 4 cut(s) 4, 35, 607, 655
Tru1I TTAA 2 cut(s) 192, 245
Tru9I TTAA 2 cut(s) 192, 245
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 371, 551, 716
Tsp45I GTSAC 1 cut(s) 385
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 45
XagI CCTNNNNNAGG 2 cut(s) 174, 687
XapI RAATTY 2 cut(s) 207, 571
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.