AT4G10955

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003376
Physical Location & Seq
Reverse (-)
6713557 .. 6715735
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10955.1

Sequence Viewer

Length: 1053 bp
ATGATGATTAGTGAAAGAGATGATTTTAGTCTCACTGGACCATTACACTTAACATCTATAGATTGGGCTAATGAACATCATCGACGATCCGTAGCTGGATCTTTGGTTCAAGGAATCTATGTAGCTGAGCGTGACCGTCAGCTACAAAGAGAAGGTCCTGAGTTAGCTTTATCTCCAATATGGTCTGAGTTTTTCCATTTCCGCCTCATTCGTAAGTTTGTCGATGACGCGGATAACTCTATCTTCGGAGGAATCTATGAGTACAAACTGCCGCAACAGCTCTCTCAAACCGTCAAATCAATGGAATTTAGTCCACGTTTTGTGATTGCTTTCAGAGGAACGGTTACAAAAGTGGACTCCATTTCCCGTGACATCGAGCATGACATCCATGTTATTAGAAACGGGCTTCACACGACAACACGGTTTGAGATAGCTATCCAAGCAGTGAGAAACATTGTTGCTTCGGTTGGTGGTTCTAGTGTTTGGCTTGCTGGTCATTCTCTTGGTGCATCTATGGCATTACTTACCGGGAAAACCATCGCTAGAACCGGGTTTTTTCCTGAGTGTTTCGCATTCAATCCGCCGTTTTTGTCTGCCCCTATCGAAAAAATTAAGGATAAGAGGATTAAACATGGGATACGCATTGCAGGCAGTGTGATCACAGCTGGACTTGCTCTAGCCAAAAAAGCCACCCAACACTACAGCCAAAACGACCGTGCATTACCCGCACCTCCTGATCCATTTGAAGCTTTATCCGACTGGTTCCCGCGGCTGTATGTCAACCCTGGTGACCACTTATGCTCAGAGTATGTTGGTTACTTTGAGCACCGAAACAAGATGGAAGAAATCGGGATTGGGTTTGTAGAGCGGGTAGCGACGCAGCACTCGTTGGGCGGTATGCTGTTAGGAGGACAAGAGCCGGTACATCTGATTCCATCTTCGGTTTTGACGGTGAACTTAAGCTCCTCGAGAGATTTCAAACAAGCTCATGGGATTCATCAGTGGTGGAGGGAAGATAACAAGTTTGAGACTAAAGTTTACCAGTACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

350

Amino Acids

39.47

Weight (kDa)

7.88

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 145 - 195 1.7e-06 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 868
AccII CGCG 2 cut(s) 230, 769
AciI CCGC 9 cut(s) 202, 230, 272, 581, 726, 767, 769, 868, 894
AclWI GGATC 3 cut(s) 81, 106, 731
AcsI RAATTY 1 cut(s) 305
AfaI GTAC 3 cut(s) 263, 924, 1046
AflII CTTAAG 1 cut(s) 958
AgsI TTSAA 4 cut(s) 110, 577, 746, 979
AjnI CCWGG 1 cut(s) 784
AjuI GAANNNNNNNTTGG 2 cut(s) 837, 869
AloI GAACNNNNNNTCC 4 cut(s) 90, 122, 947, 979
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 2 cut(s) 35, 1022
AlwI GGATC 3 cut(s) 81, 106, 731
Ama87I CYCGRG 1 cut(s) 967
ApeKI GCWGC 1 cut(s) 880
ApoI RAATTY 1 cut(s) 305
ArsI GACNNNNNNTTYG 2 cut(s) 139, 171
AspS9I GGNCC 2 cut(s) 38, 155
AsuC2I CCSGG 2 cut(s) 529, 550
AsuHPI GGTGA 2 cut(s) 800, 964
AvaI CYCGRG 1 cut(s) 967
AvaII GGWCC 2 cut(s) 38, 155
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 1 cut(s) 892
BccI CCATC 3 cut(s) 545, 832, 943
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 786
BciVI GTATCC 1 cut(s) 630
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 2 cut(s) 529, 550
BcoDI GTCTC 2 cut(s) 35, 1022
BfaI CTAG 3 cut(s) 477, 543, 677
BfmI CTRYAG 2 cut(s) 57, 700
BfrI CTTAAG 1 cut(s) 958
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 3 cut(s) 272, 770, 881
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 3 cut(s) 273, 771, 882
Bme1390I CCNGG 3 cut(s) 529, 550, 786
Bme18I GGWCC 2 cut(s) 38, 155
BmeT110I CYCGRG 1 cut(s) 967
BmgT120I GGNCC 2 cut(s) 38, 155
BmiI GGNNCC 1 cut(s) 764
BmrFI CCNGG 3 cut(s) 529, 550, 786
BmsI GCATC 1 cut(s) 518
Bpu1102I GCTNAGC 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 529, 550
BsaBI GATNNNNATC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 767, 784
BsaXI ACNNNNNCTCC 2 cut(s) 947, 977
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 3 cut(s) 40, 764, 1042
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 767, 784
BseGI GGATG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 434
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 5 cut(s) 117, 150, 177, 552, 816
BseNI ACTGG 3 cut(s) 40, 764, 1042
BseRI GAGGAG 1 cut(s) 955
BseXI GCAGC 1 cut(s) 892
Bsh1236I CGCG 2 cut(s) 230, 769
Bsh1285I CGRYCG 1 cut(s) 715
BsiEI CGRYCG 1 cut(s) 715
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 967
BsiSI CCGG 3 cut(s) 528, 549, 920
BsmAI GTCTC 2 cut(s) 35, 1022
BsmI GAATGC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 967
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 4 cut(s) 86, 98, 657, 736
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 9 cut(s) 202, 230, 272, 581, 726, 767, 769, 868, 894
BspCNI CTCAG 5 cut(s) 118, 151, 178, 553, 815
BspFNI CGCG 2 cut(s) 230, 769
BspLI GGNNCC 1 cut(s) 764
BspPI GGATC 3 cut(s) 81, 106, 731
BspTI CTTAAG 1 cut(s) 958
BsrBI CCGCTC 1 cut(s) 868
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 3 cut(s) 40, 764, 1042
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 2 cut(s) 767, 784
BssMI GATC 4 cut(s) 86, 98, 657, 736
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 6 cut(s) 137, 292, 343, 423, 716, 952
BstAFI CTTAAG 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 489, 649
BstDEI CTNAG 5 cut(s) 126, 159, 186, 561, 802
BstDSI CCRYGG 1 cut(s) 767
BstEII GGTNACC 1 cut(s) 788
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 2 cut(s) 230, 769
BstKTI GATC 4 cut(s) 89, 101, 660, 739
BstMAI GTCTC 2 cut(s) 35, 1022
BstMBI GATC 4 cut(s) 86, 98, 657, 736
BstMCI CGRYCG 1 cut(s) 715
BstMWI GCNNNNNNNGC 7 cut(s) 277, 440, 515, 648, 671, 686, 725
BstNI CCWGG 1 cut(s) 786
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 3 cut(s) 527, 548, 784
BstSFI CTRYAG 2 cut(s) 57, 700
BstUI CGCG 2 cut(s) 230, 769
BstV1I GCAGC 1 cut(s) 892
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BsuI GTATCC 1 cut(s) 630
BtgI CCRYGG 1 cut(s) 767
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 1 cut(s) 384
BtsI GCAGTG 2 cut(s) 450, 658
BtsIMutI CAGTG 4 cut(s) 33, 450, 658, 1007
Cac8I GCNNGC 2 cut(s) 489, 649
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 2 cut(s) 38, 155
Cfr42I CCGCGG 1 cut(s) 770
CseI GACGC 2 cut(s) 236, 886
Csp6I GTAC 3 cut(s) 262, 923, 1045
CviAII CATG 4 cut(s) 380, 389, 632, 989
CviQI GTAC 3 cut(s) 262, 923, 1045
DdeI CTNAG 5 cut(s) 126, 159, 186, 561, 802
DpnI GATC 4 cut(s) 88, 100, 659, 738
DpnII GATC 4 cut(s) 86, 98, 657, 736
EciI GGCGGA 2 cut(s) 191, 570
Eco47I GGWCC 2 cut(s) 38, 155
Eco88I CYCGRG 1 cut(s) 967
Eco91I GGTNACC 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 155
EcoO65I GGTNACC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 784
FaeI CATG 4 cut(s) 383, 392, 635, 992
FatI CATG 4 cut(s) 379, 388, 631, 988
FauI CCCGC 3 cut(s) 733, 774, 861
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 272, 770, 881
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 3 cut(s) 272, 770, 881
FspBI CTAG 3 cut(s) 477, 543, 677
GluI GCNGC 3 cut(s) 272, 770, 881
HapII CCGG 3 cut(s) 528, 549, 920
HgaI GACGC 2 cut(s) 236, 886
Hin1II CATG 4 cut(s) 383, 392, 635, 992
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 5 cut(s) 114, 252, 356, 931, 994
HpaII CCGG 3 cut(s) 528, 549, 920
HphI GGTGA 2 cut(s) 800, 964
Hpy166II GTNNAC 5 cut(s) 314, 355, 781, 955, 1039
Hpy188I TCNGA 6 cut(s) 187, 248, 335, 757, 805, 930
Hpy188III TCNNGA 5 cut(s) 158, 560, 734, 850, 969
Hpy8I GTNNAC 5 cut(s) 314, 355, 781, 955, 1039
Hpy99I CGWCG 2 cut(s) 87, 880
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 6 cut(s) 137, 292, 343, 423, 716, 952
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 3 cut(s) 509, 647, 719
HpyF10VI GCNNNNNNNGC 7 cut(s) 277, 440, 515, 648, 671, 686, 725
HpyF3I CTNAG 5 cut(s) 126, 159, 186, 561, 802
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 4 cut(s) 383, 392, 635, 992
Ksp22I TGATCA 1 cut(s) 657
KspI CCGCGG 1 cut(s) 770
Kzo9I GATC 4 cut(s) 86, 98, 657, 736
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 1 cut(s) 892
LweI GCATC 1 cut(s) 518
MaeI CTAG 3 cut(s) 477, 543, 677
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 5 cut(s) 131, 343, 368, 788, 815
MalI GATC 4 cut(s) 88, 100, 659, 738
MbiI CCGCTC 1 cut(s) 868
MboI GATC 4 cut(s) 86, 98, 657, 736
MboII GAAGA 4 cut(s) 235, 854, 930, 1025
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 2 cut(s) 305, 609
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 780
MnlI CCTC 8 cut(s) 215, 242, 329, 615, 741, 902, 976, 1002
MseI TTAA 4 cut(s) 50, 612, 627, 959
MspA1I CMGCKG 2 cut(s) 665, 769
MspCI CTTAAG 1 cut(s) 958
MspI CCGG 3 cut(s) 528, 549, 920
MspR9I CCNGG 3 cut(s) 529, 550, 786
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 2 cut(s) 230, 769
MwoI GCNNNNNNNGC 7 cut(s) 277, 440, 515, 648, 671, 686, 725
NciI CCSGG 2 cut(s) 529, 550
NdeII GATC 4 cut(s) 86, 98, 657, 736
NlaIII CATG 4 cut(s) 383, 392, 635, 992
NlaIV GGNNCC 1 cut(s) 764
NmuCI GTSAC 3 cut(s) 131, 368, 788
PaeR7I CTCGAG 1 cut(s) 967
PcsI WCGNNNNNNNCGW 1 cut(s) 884
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 4 cut(s) 114, 252, 931, 994
PkrI GCNGC 3 cut(s) 273, 771, 882
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 788
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 2 cut(s) 38, 155
PspPPI RGGWCCY 1 cut(s) 155
PsuI RGATCY 1 cut(s) 98
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 3 cut(s) 263, 924, 1046
RsaNI GTAC 3 cut(s) 262, 923, 1045
SacII CCGCGG 1 cut(s) 770
SaqAI TTAA 4 cut(s) 50, 612, 627, 959
SatI GCNGC 3 cut(s) 272, 770, 881
Sau3AI GATC 4 cut(s) 86, 98, 657, 736
Sau96I GGNCC 2 cut(s) 38, 155
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 3 cut(s) 529, 550, 786
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 1 cut(s) 518
SfcI CTRYAG 2 cut(s) 57, 700
Sfr274I CTCGAG 1 cut(s) 967
Sfr303I CCGCGG 1 cut(s) 770
SgrBI CCGCGG 1 cut(s) 770
SinI GGWCC 2 cut(s) 38, 155
SlaI CTCGAG 1 cut(s) 967
SmlI CTYRAG 2 cut(s) 958, 967
SmoI CTYRAG 2 cut(s) 958, 967
Sse9I AATT 2 cut(s) 305, 609
SsiI CCGC 9 cut(s) 202, 230, 272, 581, 726, 767, 769, 868, 894
SspMI CTAG 3 cut(s) 477, 543, 677
StyD4I CCNGG 3 cut(s) 527, 548, 784
TaaI ACNGT 6 cut(s) 137, 292, 343, 423, 716, 952
TaiI ACGT 1 cut(s) 319
TaqI TCGA 5 cut(s) 82, 222, 375, 603, 968
TasI AATT 2 cut(s) 305, 609
TatI WGTACW 2 cut(s) 261, 1044
TauI GCSGC 2 cut(s) 274, 772
TfiI GAWTC 4 cut(s) 114, 252, 931, 994
Tru1I TTAA 4 cut(s) 50, 612, 627, 959
Tru9I TTAA 4 cut(s) 50, 612, 627, 959
TscAI CASTG 4 cut(s) 40, 450, 658, 1007
TseFI GTSAC 3 cut(s) 131, 368, 788
TseI GCWGC 1 cut(s) 880
Tsp45I GTSAC 3 cut(s) 131, 368, 788
TspDTI ATGAA 2 cut(s) 87, 986
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 4 cut(s) 40, 450, 658, 1007
Vha464I CTTAAG 1 cut(s) 958
VpaK11BI GGWCC 2 cut(s) 38, 155
XapI RAATTY 1 cut(s) 305
XhoI CTCGAG 1 cut(s) 967
XspI CTAG 3 cut(s) 477, 543, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.