RchiOBHm_Chr7g0194501

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003376
Physical Location & Seq
Reverse (-)
12529216 .. 12531505
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17393

Sequence Viewer

Length: 1179 bp
ATGATGATGATGATGAGCAATATTTGTGTGGGTTATTATCACGGAGTATTTGATAGTTCTGGAAAAGAGAAAGGGTATATCAAGAGCAAGCTTAGAGAGCAACGTTGGTGTCAAATGGTTTCTGATAAGCATCTCTTTTATGTTTCAGGACCAACACATCTGACTTCTATTGACTGGAAGAACAGTGATCATCAGAGGTCTGTTGCTGCCAGTTTGGTTAAGGGTGTCAAAGTTCTTGAGCTGGACCGCCAAGAGAAGCGTGAAGGACCCGAGGCCTTGGCTCCTCCTTGGTGGGAGTTCTTCCATTTCAAGTTGCTTCGTCCACTGGTGGATGATGCCAGTTGTGGTTCCATTTTCGGTGCCATTTATGAGTTTAAACCTCCTCATGCTCCTCCTTTCTCTTCTCTTGGTACAGAACATTCACTATTGGAAGATGAAAACAGCCCTCATTATGTTATTGCCTTCCGTGGCACCTTAACCGCGTATGACCTGGTCTCGCGAGATCTTAACTTGTCCCTCAAGTTAATCAAAAATGGACTTCACAAGACATCTCGCTTTAAGACTGCTATGCAAGCAATCCAAGCTACGGTTGGGGAAGCAGGTGATTCATCAAGCGTTTGGCTAGCTGGCCATTCCATGGGTTCAGTATTGGCAATGCTTGCTGGAAAAACAATGGCCAAGGACGGAATCTTACTCAAATCTTTTCTCTTCAATCAACCATTTGTTTTCGTTGCCCCGATGGAGAGGATCAAACACGAGAATATCAAGCTTGGTATTCGCTTTGCCCGGAGTGTGTTCACTGCGGGACTGGCTAAAGCTGTAAATCCCACCTGGTCAGAACACGAAGATCCATTCTTCGGTCTTTATGAATGGGTTCCTAGTGTATTTGTGAATCAAGGTGACGACTTCTGCTCTGGATATATTGGTTATTTCGAACATAGAAAGAAGATGGTAGAGATTGGAGCAGGAGGCATTGAGAGGTTAGCAACCCAGAATTCAATTGGGAGTTTATTGAGGTATGCAATAGGAAAGGAGACAGAGCCACCAATGCACCTCATTCCTTGTGCAAACCTCTCAGTCAATTTAACCCCTTCAAGGAATTTCAAAGAAGCCCATGGAATTCGACAATGGTGGCTAGAGAATCAGCACTTGCAGTCTGAGACTTACAGATACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

392

Amino Acids

44.3

Weight (kDa)

8.63

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 590
AccB1I GGYRCC 2 cut(s) 359, 470
AccB7I CCANNNNNTGG 1 cut(s) 637
AccII CGCG 2 cut(s) 482, 499
AciI CCGC 3 cut(s) 247, 480, 803
AclI AACGTT 1 cut(s) 103
AclWI GGATC 2 cut(s) 755, 842
AcoI YGGCCR 2 cut(s) 628, 675
AcsI RAATTY 3 cut(s) 994, 1099, 1119
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 4 cut(s) 586, 637, 857, 1095
AgsI TTSAA 5 cut(s) 310, 712, 999, 1095, 1105
AjnI CCWGG 2 cut(s) 489, 830
AluBI AGCT 6 cut(s) 91, 241, 584, 626, 769, 818
AluI AGCT 6 cut(s) 91, 241, 584, 626, 769, 818
Alw26I GTCTC 3 cut(s) 499, 1028, 1154
AlwI GGATC 2 cut(s) 755, 842
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 3 cut(s) 273, 628, 675
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 3 cut(s) 994, 1099, 1119
Asp700I GAANNNNTTC 1 cut(s) 873
AspS9I GGNCC 3 cut(s) 149, 244, 266
AsuC2I CCSGG 1 cut(s) 787
AsuHPI GGTGA 2 cut(s) 614, 911
AsuII TTCGAA 1 cut(s) 933
AsuNHI GCTAGC 1 cut(s) 622
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 3 cut(s) 149, 244, 266
BalI TGGCCA 2 cut(s) 630, 677
BanI GGYRCC 2 cut(s) 359, 470
BauI CACGAG 1 cut(s) 755
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 733, 943
BciT130I CCWGG 2 cut(s) 491, 832
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 1 cut(s) 787
BcoDI GTCTC 3 cut(s) 499, 1028, 1154
BfaI CTAG 3 cut(s) 623, 879, 1136
BfuAI ACCTGC 1 cut(s) 590
BglII AGATCT 1 cut(s) 502
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 3 cut(s) 491, 787, 832
Bme18I GGWCC 3 cut(s) 149, 244, 266
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 149, 244, 266
BmiI GGNNCC 6 cut(s) 268, 282, 349, 361, 472, 876
BmrFI CCNGG 3 cut(s) 491, 787, 832
BmsI GCATC 2 cut(s) 139, 325
BmtI GCTAGC 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 2 cut(s) 257, 503
BpuMI CCSGG 1 cut(s) 787
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 7 cut(s) 270, 276, 287, 466, 636, 678, 1114
Bsc4I CCNNNNNNNGG 4 cut(s) 586, 637, 857, 1095
Bse1I ACTGG 5 cut(s) 179, 210, 330, 339, 813
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 2 cut(s) 491, 832
BseDI CCNNGG 7 cut(s) 270, 276, 287, 466, 636, 678, 1114
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 4 cut(s) 586, 637, 857, 1095
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 1089, 1149
BseNI ACTGG 5 cut(s) 179, 210, 330, 339, 813
BseRI GAGGAG 3 cut(s) 273, 372, 381
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 482, 499
BshFI GGCC 3 cut(s) 275, 630, 677
BshNI GGYRCC 2 cut(s) 359, 470
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 787
BslFI GGGAC 2 cut(s) 499, 819
BslI CCNNNNNNNGG 4 cut(s) 586, 637, 857, 1095
BsmAI GTCTC 3 cut(s) 499, 1028, 1154
BsmFI GGGAC 2 cut(s) 499, 819
BsnI GGCC 3 cut(s) 275, 630, 677
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 933
Bsp143I GATC 4 cut(s) 187, 502, 747, 847
Bsp19I CCATGG 2 cut(s) 636, 1114
Bsp68I TCGCGA 1 cut(s) 499
BspACI CCGC 3 cut(s) 247, 480, 803
BspANI GGCC 3 cut(s) 275, 630, 677
BspCNI CTCAG 2 cut(s) 1088, 1150
BspFNI CGCG 2 cut(s) 482, 499
BspLI GGNNCC 6 cut(s) 268, 282, 349, 361, 472, 876
BspMI ACCTGC 1 cut(s) 590
BspOI GCTAGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 755, 842
BspT104I TTCGAA 1 cut(s) 933
BspT107I GGYRCC 2 cut(s) 359, 470
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 5 cut(s) 179, 210, 330, 339, 813
BssECI CCNNGG 7 cut(s) 270, 276, 287, 466, 636, 678, 1114
BssMI GATC 4 cut(s) 187, 502, 747, 847
BssSI CACGAG 1 cut(s) 755
BssT1I CCWWGG 5 cut(s) 276, 287, 636, 678, 1114
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 2 cut(s) 491, 832
Bst4CI ACNGT 2 cut(s) 185, 589
Bst6I CTCTTC 2 cut(s) 406, 713
BstAPI GCANNNNNTGC 1 cut(s) 659
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 5 cut(s) 89, 573, 624, 628, 660
BstDEI CTNAG 3 cut(s) 92, 1075, 1158
BstDSI CCRYGG 3 cut(s) 466, 636, 1114
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 2 cut(s) 482, 499
BstKTI GATC 4 cut(s) 190, 505, 750, 850
BstMAI GTCTC 3 cut(s) 499, 1028, 1154
BstMBI GATC 4 cut(s) 187, 502, 747, 847
BstMWI GCNNNNNNNGC 6 cut(s) 97, 572, 581, 659, 809, 1048
BstNI CCWGG 2 cut(s) 491, 832
BstSCI CCNGG 3 cut(s) 489, 785, 830
BstUI CGCG 2 cut(s) 482, 499
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 502, 847
BstYI RGATCY 2 cut(s) 502, 847
BsuRI GGCC 3 cut(s) 275, 630, 677
BtgI CCRYGG 3 cut(s) 466, 636, 1114
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 3 cut(s) 190, 323, 798
BtuMI TCGCGA 1 cut(s) 499
BveI ACCTGC 1 cut(s) 590
Cac8I GCNNGC 5 cut(s) 89, 573, 624, 628, 660
Cfr13I GGNCC 3 cut(s) 149, 244, 266
CsiI ACCWGGT 2 cut(s) 489, 830
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 386, 637, 1115
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 92, 1075, 1158
DpnI GATC 4 cut(s) 189, 504, 749, 849
DpnII GATC 4 cut(s) 187, 502, 747, 847
DraI TTTAAA 1 cut(s) 376
EaeI YGGCCR 2 cut(s) 628, 675
Eam1104I CTCTTC 2 cut(s) 406, 713
EarI CTCTTC 2 cut(s) 406, 713
Eco130I CCWWGG 5 cut(s) 276, 287, 636, 678, 1114
Eco147I AGGCCT 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 499
Eco47I GGWCC 3 cut(s) 149, 244, 266
Eco88I CYCGRG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 266
EcoRI GAATTC 2 cut(s) 994, 1119
EcoRII CCWGG 2 cut(s) 489, 830
EcoT14I CCWWGG 5 cut(s) 276, 287, 636, 678, 1114
ErhI CCWWGG 5 cut(s) 276, 287, 636, 678, 1114
FaeI CATG 3 cut(s) 389, 640, 1118
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 2 cut(s) 499, 819
FatI CATG 3 cut(s) 385, 636, 1114
FauI CCCGC 1 cut(s) 796
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 3 cut(s) 623, 879, 1136
GluI GCNGC 1 cut(s) 207
HaeIII GGCC 3 cut(s) 275, 630, 677
HapII CCGG 1 cut(s) 787
Hin1II CATG 3 cut(s) 389, 640, 1118
HindIII AAGCTT 2 cut(s) 89, 767
HinfI GANTC 4 cut(s) 605, 687, 892, 1141
HpaII CCGG 1 cut(s) 787
HphI GGTGA 2 cut(s) 614, 911
Hpy166II GTNNAC 2 cut(s) 323, 798
Hpy188I TCNGA 5 cut(s) 124, 162, 195, 838, 1159
Hpy188III TCNNGA 6 cut(s) 60, 82, 147, 236, 498, 915
Hpy8I GTNNAC 2 cut(s) 323, 798
HpyAV CCTTC 3 cut(s) 257, 472, 1101
HpyCH4III ACNGT 2 cut(s) 185, 589
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 5 cut(s) 571, 1022, 1051, 1067, 1153
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 572, 581, 659, 809, 1048
HpyF3I CTNAG 3 cut(s) 92, 1075, 1158
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 3 cut(s) 389, 640, 1118
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 4 cut(s) 187, 502, 747, 847
LmnI GCTCC 3 cut(s) 286, 394, 962
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 139, 325
MabI ACCWGGT 2 cut(s) 489, 830
MaeI CTAG 3 cut(s) 623, 879, 1136
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 1 cut(s) 899
MalI GATC 4 cut(s) 189, 504, 749, 849
MboI GATC 4 cut(s) 187, 502, 747, 847
MboII GAAGA 8 cut(s) 190, 292, 393, 443, 700, 847, 857, 958
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 2 cut(s) 502, 847
MlsI TGGCCA 2 cut(s) 630, 677
MluCI AATT 5 cut(s) 994, 999, 1081, 1099, 1119
MluNI TGGCCA 2 cut(s) 630, 677
Mox20I TGGCCA 2 cut(s) 630, 677
MroXI GAANNNNTTC 1 cut(s) 873
MscI TGGCCA 2 cut(s) 630, 677
MseI TTAA 7 cut(s) 219, 375, 476, 507, 524, 558, 1085
Msp20I TGGCCA 2 cut(s) 630, 677
MspI CCGG 1 cut(s) 787
MspR9I CCNGG 3 cut(s) 491, 787, 832
MssI GTTTAAAC 1 cut(s) 376
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 2 cut(s) 491, 832
MvnI CGCG 2 cut(s) 482, 499
MwoI GCNNNNNNNGC 6 cut(s) 97, 572, 581, 659, 809, 1048
NciI CCSGG 1 cut(s) 787
NcoI CCATGG 2 cut(s) 636, 1114
NdeII GATC 4 cut(s) 187, 502, 747, 847
NheI GCTAGC 1 cut(s) 622
NlaIII CATG 3 cut(s) 389, 640, 1118
NlaIV GGNNCC 6 cut(s) 268, 282, 349, 361, 472, 876
NmuCI GTSAC 1 cut(s) 899
NruI TCGCGA 1 cut(s) 499
NspV TTCGAA 1 cut(s) 933
PaqCI CACCTGC 1 cut(s) 590
PceI AGGCCT 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 873
PfeI GAWTC 4 cut(s) 605, 687, 892, 1141
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 637
PkrI GCNGC 1 cut(s) 208
PmeI GTTTAAAC 1 cut(s) 376
PpuMI RGGWCCY 1 cut(s) 266
Psp1406I AACGTT 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 489, 830
PspGI CCWGG 2 cut(s) 489, 830
PspN4I GGNNCC 6 cut(s) 268, 282, 349, 361, 472, 876
PspPI GGNCC 3 cut(s) 149, 244, 266
PspPPI RGGWCCY 1 cut(s) 266
PsuI RGATCY 2 cut(s) 502, 847
PsyI GACNNNGTC 1 cut(s) 491
RruI TCGCGA 1 cut(s) 499
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SaqAI TTAA 7 cut(s) 219, 375, 476, 507, 524, 558, 1085
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 187, 502, 747, 847
Sau96I GGNCC 3 cut(s) 149, 244, 266
ScrFI CCNGG 3 cut(s) 491, 787, 832
SexAI ACCWGGT 2 cut(s) 489, 830
SfaNI GCATC 2 cut(s) 139, 325
SfuI TTCGAA 1 cut(s) 933
SinI GGWCC 3 cut(s) 149, 244, 266
SmlI CTYRAG 2 cut(s) 236, 518
SmoI CTYRAG 2 cut(s) 236, 518
Sse9I AATT 5 cut(s) 994, 999, 1081, 1099, 1119
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 3 cut(s) 247, 480, 803
SspI AATATT 1 cut(s) 22
SspMI CTAG 3 cut(s) 623, 879, 1136
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 489, 785, 830
StyI CCWWGG 5 cut(s) 276, 287, 636, 678, 1114
TaaI ACNGT 2 cut(s) 185, 589
TaiI ACGT 1 cut(s) 106
TaqI TCGA 2 cut(s) 933, 1123
TaqII GACCGA 1 cut(s) 848
TasI AATT 5 cut(s) 994, 999, 1081, 1099, 1119
TfiI GAWTC 4 cut(s) 605, 687, 892, 1141
Tru1I TTAA 7 cut(s) 219, 375, 476, 507, 524, 558, 1085
Tru9I TTAA 7 cut(s) 219, 375, 476, 507, 524, 558, 1085
TscAI CASTG 3 cut(s) 190, 330, 805
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 3 cut(s) 450, 597, 882
TspGWI ACGGA 3 cut(s) 57, 455, 699
TspRI CASTG 3 cut(s) 190, 330, 805
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 637
VpaK11BI GGWCC 3 cut(s) 149, 244, 266
XapI RAATTY 3 cut(s) 994, 1099, 1119
XcmI CCANNNNNNNNNTGG 2 cut(s) 587, 998
XmnI GAANNNNTTC 1 cut(s) 873
XspI CTAG 3 cut(s) 623, 879, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.