Rmu_sc0041088.1_g000001

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0041088.1
OG0003376
Physical Location & Seq
Forward (+)
553 .. 2021
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0041088.1_g000001.1.cds

Sequence Viewer

Length: 532 bp
atgatgatgatgagcaatatttgtgtgggttattatcacggagtatttgatagttctggaaaagagaaagggtatatcaagagcaagcttagagagcaacgttggtgtcaaatggtttctgataagcatctcttttatgtttcaggaccaacacatctgacttctattgactggaagaacagtgatcatcagaggtctgttgctgccagtttggttaagggtgtcaaagttcttgagctggaccgccaagagaagcgtgaaggacccgaggccttggctcctccttggtgggagttcttccatttcaagttgcttcgtccactggtggatgatgccagttgtggttccattttcggtgccatttatgagtttaaacctcctcatgctcctcctttctcttctcttggtacagaacatttactattggaagatgaaaacagccctcattatgttattgccttccgtggcaccttaaccgcgtatgacctggtctcgcgagatcttaacttgtccctcaagttaatcaaaaatg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

177

Amino Acids

20.23

Weight (kDa)

7.09

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 356, 467
AccII CGCG 2 cut(s) 479, 496
AciI CCGC 2 cut(s) 244, 477
AclI AACGTT 1 cut(s) 100
AfaI GTAC 1 cut(s) 409
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 1 cut(s) 486
AluBI AGCT 2 cut(s) 88, 238
AluI AGCT 2 cut(s) 88, 238
Alw26I GTCTC 1 cut(s) 496
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 146, 241, 263
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 3 cut(s) 146, 241, 263
BanI GGYRCC 2 cut(s) 356, 467
BbvI GCAGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 488
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 496
BglII AGATCT 1 cut(s) 499
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme1390I CCNGG 1 cut(s) 488
Bme18I GGWCC 3 cut(s) 146, 241, 263
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 146, 241, 263
BmiI GGNNCC 5 cut(s) 265, 279, 346, 358, 469
BmrFI CCNGG 1 cut(s) 488
BmsI GCATC 2 cut(s) 136, 322
BpuEI CTTGAG 2 cut(s) 254, 500
BsaBI GATNNNNATC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 267, 273, 284, 463
Bse1I ACTGG 4 cut(s) 176, 207, 327, 336
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 4 cut(s) 267, 273, 284, 463
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 126
BseNI ACTGG 4 cut(s) 176, 207, 327, 336
BseRI GAGGAG 3 cut(s) 270, 369, 378
BseXI GCAGC 1 cut(s) 190
Bsh1236I CGCG 2 cut(s) 479, 496
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 2 cut(s) 356, 467
BsiHKCI CYCGRG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 496
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 496
BsoBI CYCGRG 1 cut(s) 266
Bsp143I GATC 2 cut(s) 184, 499
Bsp68I TCGCGA 1 cut(s) 496
BspACI CCGC 2 cut(s) 244, 477
BspANI GGCC 1 cut(s) 272
BspFNI CGCG 2 cut(s) 479, 496
BspLI GGNNCC 5 cut(s) 265, 279, 346, 358, 469
BspT107I GGYRCC 2 cut(s) 356, 467
BspTNI GGTCTC 1 cut(s) 496
BsrI ACTGG 4 cut(s) 176, 207, 327, 336
BssECI CCNNGG 4 cut(s) 267, 273, 284, 463
BssMI GATC 2 cut(s) 184, 499
BssT1I CCWWGG 2 cut(s) 273, 284
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 1 cut(s) 89
BstDSI CCRYGG 1 cut(s) 463
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 2 cut(s) 479, 496
BstKTI GATC 2 cut(s) 187, 502
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 2 cut(s) 184, 499
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 486
BstUI CGCG 2 cut(s) 479, 496
BstV1I GCAGC 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 1 cut(s) 272
BtgI CCRYGG 1 cut(s) 463
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 187, 320
BtuMI TCGCGA 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 86
Cfr13I GGNCC 3 cut(s) 146, 241, 263
CsiI ACCWGGT 1 cut(s) 486
Csp6I GTAC 1 cut(s) 408
CviAII CATG 1 cut(s) 383
CviJI RGCY 5 cut(s) 88, 238, 272, 278, 441
CviKI_1 RGCY 5 cut(s) 88, 238, 272, 278, 441
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 1 cut(s) 89
DpnI GATC 2 cut(s) 186, 501
DpnII GATC 2 cut(s) 184, 499
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 273, 284
Eco147I AGGCCT 1 cut(s) 272
Eco31I GGTCTC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 146, 241, 263
Eco88I CYCGRG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 486
EcoT14I CCWWGG 2 cut(s) 273, 284
ErhI CCWWGG 2 cut(s) 273, 284
FaeI CATG 1 cut(s) 386
FaiI YATR 6 cut(s) 75, 138, 366, 384, 450, 483
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 1 cut(s) 496
FatI CATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 204
GluI GCNGC 1 cut(s) 204
HaeIII GGCC 1 cut(s) 272
Hin1II CATG 1 cut(s) 386
HindIII AAGCTT 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 121, 159, 192
Hpy188III TCNNGA 5 cut(s) 57, 79, 144, 233, 495
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 2 cut(s) 254, 469
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 1 cut(s) 89
HpySE526I ACGT 1 cut(s) 100
Hsp92II CATG 1 cut(s) 386
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 184, 499
LmnI GCTCC 2 cut(s) 283, 391
LpnPI CCDG 9 cut(s) 42, 129, 157, 220, 224, 308, 349, 473, 500
Lsp1109I GCAGC 1 cut(s) 190
LweI GCATC 2 cut(s) 136, 322
MabI ACCWGGT 1 cut(s) 486
MaeII ACGT 1 cut(s) 100
MalI GATC 2 cut(s) 186, 501
MboI GATC 2 cut(s) 184, 499
MboII GAAGA 4 cut(s) 187, 289, 390, 440
MflI RGATCY 1 cut(s) 499
MnlI CCTC 8 cut(s) 186, 262, 291, 387, 390, 399, 453, 524
MseI TTAA 5 cut(s) 216, 372, 473, 504, 521
MspR9I CCNGG 1 cut(s) 488
MssI GTTTAAAC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 488
MvnI CGCG 2 cut(s) 479, 496
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeII GATC 2 cut(s) 184, 499
NlaIII CATG 1 cut(s) 386
NlaIV GGNNCC 5 cut(s) 265, 279, 346, 358, 469
NruI TCGCGA 1 cut(s) 496
PceI AGGCCT 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 488
PkrI GCNGC 1 cut(s) 205
PmeI GTTTAAAC 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 263
Psp1406I AACGTT 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 5 cut(s) 265, 279, 346, 358, 469
PspPI GGNCC 3 cut(s) 146, 241, 263
PspPPI RGGWCCY 1 cut(s) 263
PsuI RGATCY 1 cut(s) 499
PsyI GACNNNGTC 1 cut(s) 488
RruI TCGCGA 1 cut(s) 496
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SaqAI TTAA 5 cut(s) 216, 372, 473, 504, 521
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 2 cut(s) 184, 499
Sau96I GGNCC 3 cut(s) 146, 241, 263
ScrFI CCNGG 1 cut(s) 488
SetI ASST 7 cut(s) 90, 103, 197, 240, 379, 473, 489
SexAI ACCWGGT 1 cut(s) 486
SfaNI GCATC 2 cut(s) 136, 322
SinI GGWCC 3 cut(s) 146, 241, 263
SmlI CTYRAG 2 cut(s) 233, 515
SmoI CTYRAG 2 cut(s) 233, 515
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 2 cut(s) 244, 477
SspI AATATT 1 cut(s) 19
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 486
StyI CCWWGG 2 cut(s) 273, 284
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 103
Tru1I TTAA 5 cut(s) 216, 372, 473, 504, 521
Tru9I TTAA 5 cut(s) 216, 372, 473, 504, 521
TscAI CASTG 2 cut(s) 187, 327
TseI GCWGC 1 cut(s) 203
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 2 cut(s) 54, 452
TspRI CASTG 2 cut(s) 187, 327
Tth111I GACNNNGTC 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 146, 241, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.