FvH4_5g04751

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003376
Physical Location & Seq
Forward (+)
2787388 .. 2789989
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04751.t1

Sequence Viewer

Length: 1053 bp
ATGGCTTCCGAGAGGGAATGTTTTGAACTTTCAGGACCATTGCACCTAACTTGCATTGACTGGGAGAACACTAATCATCAAAGGTCTGTTGCTGCTAGTTTGGTTCAGGGTGTCTACATTGTTGAGCGTGACCGCCAAGAGAAGCGTGAAGGTTCTGAGGCCCTTGCTCCTCCTTGGTGGGAGTTCTTTCATTTTAAGTTGCTCCGTAAACTAGTGGATGATGTTGGTTTATCCATCTTTGGTGCCATTTATGAATTCAAGCCTCCTCCGTCTTTGCGCCACCACTCACTAGAGGGGAGACCTTGTTATGTCATTGCCTTCCGTGGCACTGTGACCAAGTCCGACTCTGTCTCACGAGATCTGGAGTTGGATGTTGAGGTCATCCGCAATGGACTTCACCGAACGTCTCGTTTTGAGATTGCTATACAAGCTGTCAGAAATATGGTTGCTGCTGTAGGGGATTCAAATGTTTGGTTAGCTGGTCATTCCTTAGGGTCATCCATGGCAATGCTTGCTGGGAAAACTATGGCCAGGAATGGCGCTAAACTCAAGTCTTTCCTCTTTAATCCACCGTTTGTTTCTGCCCCGATTGAGAGGATTACTAATAAGAATGTGAAGCATGGGATTCGATTTGCAGGCAGTGTAATTACTGCCGGGCTGTCTTTTGCTATGAAGGCTAAACAACAGCAACATCAGGGAAATCGATCAGTTGAAGACCCATTCATGACTCTGTCTTCATGGTTCCCTAGTCTGTTTGTCAATCCAGGAGATGACATATGCTCGGAATATATTGGCTATTTTGAGCATAGGAAGAGGATGGAGCAGATTGGAGCTGGGGCCATTGAGAGGTTAGCTACCCAGAATTCGCTCGGAGGTCTACTAATGCATGCAGTGGGAAAAGCCTCAGATTCCGAGCCAGCATTGCACCTCATTCCTTCTGCCAACCTGACTGTAAATCTAACCCCTGCACGGGATCTCAAAGAAGCTCATGGAATTCACCAATGGTGGGCAGACAGTATGCAGTTGAGGTCTGAGGTCCACAGATACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

351

Amino Acids

39.13

Weight (kDa)

8.62

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 142 - 191 3.9e-06 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccI GTMKAC 2 cut(s) 114, 877
AciI CCGC 2 cut(s) 133, 385
AclWI GGATC 1 cut(s) 981
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 3 cut(s) 254, 862, 993
AfiI CCNNNNNNNGG 4 cut(s) 846, 969, 970, 1006
AgsI TTSAA 4 cut(s) 26, 259, 465, 713
AhlI ACTAGT 1 cut(s) 211
AjnI CCWGG 2 cut(s) 530, 763
AluBI AGCT 5 cut(s) 431, 479, 833, 854, 986
AluI AGCT 5 cut(s) 431, 479, 833, 854, 986
Alw26I GTCTC 3 cut(s) 292, 355, 411
AlwI GGATC 1 cut(s) 981
AoxI GGCC 3 cut(s) 159, 528, 837
ApeKI GCWGC 2 cut(s) 92, 449
ApoI RAATTY 3 cut(s) 254, 862, 993
AspLEI GCGC 2 cut(s) 279, 542
AspS9I GGNCC 4 cut(s) 35, 160, 837, 1036
AsuC2I CCSGG 1 cut(s) 655
AsuHPI GGTGA 2 cut(s) 389, 989
AvaII GGWCC 2 cut(s) 35, 1036
AxyI CCTNAGG 1 cut(s) 490
BalI TGGCCA 1 cut(s) 530
BanI GGYRCC 1 cut(s) 242
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 2 cut(s) 720, 726
BbvI GCAGC 2 cut(s) 79, 436
BccI CCATC 2 cut(s) 242, 811
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 2 cut(s) 532, 765
BcnI CCSGG 1 cut(s) 655
BcoDI GTCTC 3 cut(s) 292, 355, 411
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 4 cut(s) 96, 212, 290, 747
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 543
BglII AGATCT 1 cut(s) 358
BisI GCNGC 2 cut(s) 93, 450
BlsI GCNGC 2 cut(s) 94, 451
Bme1390I CCNGG 3 cut(s) 532, 655, 765
Bme18I GGWCC 2 cut(s) 35, 1036
BmgT120I GGNCC 4 cut(s) 35, 160, 837, 1036
BmiI GGNNCC 3 cut(s) 244, 743, 838
BmrFI CCNGG 3 cut(s) 532, 655, 765
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 2 cut(s) 720, 726
BpmI CTGGAG 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 655
Bsa29I ATCGAT 1 cut(s) 703
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 173, 322, 501
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 4 cut(s) 846, 969, 970, 1006
Bse1I ACTGG 1 cut(s) 65
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 5 cut(s) 38, 312, 394, 513, 920
BseBI CCWGG 2 cut(s) 532, 765
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 3 cut(s) 173, 322, 501
BseGI GGATG 5 cut(s) 223, 376, 381, 497, 822
BseLI CCNNNNNNNGG 4 cut(s) 846, 969, 970, 1006
BseMI GCAATG 5 cut(s) 38, 312, 394, 513, 920
BseMII CTCAG 3 cut(s) 147, 918, 1023
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 2 cut(s) 159, 255
BseXI GCAGC 2 cut(s) 79, 436
BseYI CCCAGC 2 cut(s) 515, 833
BsgI GTGCAG 1 cut(s) 951
BshFI GGCC 3 cut(s) 161, 530, 839
BshNI GGYRCC 1 cut(s) 242
BshVI ATCGAT 1 cut(s) 703
BsiSI CCGG 1 cut(s) 654
BslI CCNNNNNNNGG 4 cut(s) 846, 969, 970, 1006
BsmAI GTCTC 3 cut(s) 292, 355, 411
BsmBI CGTCTC 1 cut(s) 411
BsnI GGCC 3 cut(s) 161, 530, 839
Bso31I GGTCTC 1 cut(s) 292
Bsp143I GATC 3 cut(s) 358, 704, 973
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 2 cut(s) 133, 385
BspANI GGCC 3 cut(s) 161, 530, 839
BspCNI CTCAG 3 cut(s) 148, 917, 1024
BspDI ATCGAT 1 cut(s) 703
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 3 cut(s) 244, 743, 838
BspPI GGATC 1 cut(s) 981
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 292
BsrDI GCAATG 5 cut(s) 38, 312, 394, 513, 920
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 173, 322, 501
BssMI GATC 3 cut(s) 358, 704, 973
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 173, 501
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 532, 765
Bst4CI ACNGT 4 cut(s) 331, 573, 952, 1016
Bst6I CTCTTC 1 cut(s) 806
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 4 cut(s) 513, 637, 888, 918
BstDEI CTNAG 4 cut(s) 156, 490, 904, 1032
BstDSI CCRYGG 2 cut(s) 322, 501
BstF5I GGATG 5 cut(s) 223, 376, 381, 497, 822
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 279, 542
BstKTI GATC 3 cut(s) 361, 707, 976
BstMAI GTCTC 3 cut(s) 292, 355, 411
BstMBI GATC 3 cut(s) 358, 704, 973
BstMWI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
BstNI CCWGG 2 cut(s) 532, 765
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 3 cut(s) 530, 653, 763
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 2 cut(s) 79, 436
BstV2I GAAGAC 2 cut(s) 720, 726
BstX2I RGATCY 2 cut(s) 358, 973
BstYI RGATCY 2 cut(s) 358, 973
Bsu15I ATCGAT 1 cut(s) 703
Bsu36I CCTNAGG 1 cut(s) 490
BsuRI GGCC 3 cut(s) 161, 530, 839
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 2 cut(s) 322, 501
BtsCI GGATG 5 cut(s) 223, 376, 381, 497, 822
BtsI GCAGTG 2 cut(s) 646, 897
BtsIMutI CAGTG 3 cut(s) 327, 646, 897
Cac8I GCNNGC 4 cut(s) 513, 637, 888, 918
CciI TCATGA 1 cut(s) 723
CfoI GCGC 2 cut(s) 279, 542
Cfr13I GGNCC 4 cut(s) 35, 160, 837, 1036
ClaI ATCGAT 1 cut(s) 703
CviAII CATG 6 cut(s) 502, 620, 724, 738, 887, 989
DdeI CTNAG 4 cut(s) 156, 490, 904, 1032
DpnI GATC 3 cut(s) 360, 706, 975
DpnII GATC 3 cut(s) 358, 704, 973
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
Eco130I CCWWGG 2 cut(s) 173, 501
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 35, 1036
Eco81I CCTNAGG 1 cut(s) 490
EcoO109I RGGNCCY 1 cut(s) 160
EcoRI GAATTC 3 cut(s) 254, 862, 993
EcoRII CCWGG 2 cut(s) 530, 763
EcoT14I CCWWGG 2 cut(s) 173, 501
EcoT22I ATGCAT 1 cut(s) 888
ErhI CCWWGG 2 cut(s) 173, 501
Esp3I CGTCTC 1 cut(s) 411
FaeI CATG 6 cut(s) 505, 623, 727, 741, 890, 992
FatI CATG 6 cut(s) 501, 619, 723, 737, 886, 988
FauNDI CATATG 1 cut(s) 776
FblI GTMKAC 2 cut(s) 114, 877
Fnu4HI GCNGC 2 cut(s) 93, 450
FokI GGATG 5 cut(s) 230, 368, 383, 484, 829
Fsp4HI GCNGC 2 cut(s) 93, 450
FspBI CTAG 4 cut(s) 96, 212, 290, 747
GlaI GCGC 2 cut(s) 278, 541
GluI GCNGC 2 cut(s) 93, 450
GsaI CCCAGC 2 cut(s) 519, 837
GsuI CTGGAG 1 cut(s) 383
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 3 cut(s) 161, 530, 839
HapII CCGG 1 cut(s) 654
HhaI GCGC 2 cut(s) 279, 542
Hin1II CATG 6 cut(s) 505, 623, 727, 741, 890, 992
Hin6I GCGC 2 cut(s) 277, 540
HinP1I GCGC 2 cut(s) 277, 540
HinfI GANTC 5 cut(s) 344, 461, 625, 727, 908
HpaII CCGG 1 cut(s) 654
HphI GGTGA 2 cut(s) 389, 989
Hpy166II GTNNAC 4 cut(s) 115, 209, 878, 1039
Hpy188I TCNGA 9 cut(s) 10, 157, 343, 437, 784, 872, 907, 913, 1033
Hpy188III TCNNGA 4 cut(s) 33, 354, 362, 724
Hpy8I GTNNAC 4 cut(s) 115, 209, 878, 1039
HpyAV CCTTC 4 cut(s) 143, 328, 667, 945
HpyCH4III ACNGT 4 cut(s) 331, 573, 952, 1016
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 8 cut(s) 43, 54, 635, 886, 890, 925, 968, 1021
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
HpyF3I CTNAG 4 cut(s) 156, 490, 904, 1032
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 6 cut(s) 505, 623, 727, 741, 890, 992
HspAI GCGC 2 cut(s) 277, 540
Kzo9I GATC 3 cut(s) 358, 704, 973
LmnI GCTCC 4 cut(s) 172, 207, 820, 830
Lsp1109I GCAGC 2 cut(s) 79, 436
MaeI CTAG 4 cut(s) 96, 212, 290, 747
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 2 cut(s) 128, 331
MalI GATC 3 cut(s) 360, 706, 975
MboI GATC 3 cut(s) 358, 704, 973
MboII GAAGA 3 cut(s) 725, 726, 823
MflI RGATCY 2 cut(s) 358, 973
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 4 cut(s) 254, 645, 862, 993
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 2 cut(s) 338, 721
MmeI TCCRAC 2 cut(s) 348, 366
Mox20I TGGCCA 1 cut(s) 530
Mph1103I ATGCAT 1 cut(s) 888
MscI TGGCCA 1 cut(s) 530
MseI TTAA 2 cut(s) 195, 564
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 1 cut(s) 530
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 3 cut(s) 532, 655, 765
MvaI CCWGG 2 cut(s) 532, 765
MwoI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
NciI CCSGG 1 cut(s) 655
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 776
NdeII GATC 3 cut(s) 358, 704, 973
NlaIII CATG 6 cut(s) 505, 623, 727, 741, 890, 992
NlaIV GGNNCC 3 cut(s) 244, 743, 838
NmuCI GTSAC 2 cut(s) 128, 331
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 1 cut(s) 890
PaeI GCATGC 1 cut(s) 890
PagI TCATGA 1 cut(s) 723
PfeI GAWTC 3 cut(s) 461, 625, 908
PflFI GACNNNGTC 3 cut(s) 337, 347, 730
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 2 cut(s) 94, 451
PleI GAGTC 2 cut(s) 338, 721
PpsI GAGTC 2 cut(s) 338, 721
Psp6I CCWGG 2 cut(s) 530, 763
PspFI CCCAGC 2 cut(s) 515, 833
PspGI CCWGG 2 cut(s) 530, 763
PspN4I GGNNCC 3 cut(s) 244, 743, 838
PspPI GGNCC 4 cut(s) 35, 160, 837, 1036
PsuI RGATCY 2 cut(s) 358, 973
PsyI GACNNNGTC 3 cut(s) 337, 347, 730
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 2 cut(s) 195, 564
SatI GCNGC 2 cut(s) 93, 450
Sau3AI GATC 3 cut(s) 358, 704, 973
Sau96I GGNCC 4 cut(s) 35, 160, 837, 1036
SchI GAGTC 2 cut(s) 338, 721
ScrFI CCNGG 3 cut(s) 532, 655, 765
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 2 cut(s) 35, 1036
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SpeI ACTAGT 1 cut(s) 211
SphI GCATGC 1 cut(s) 890
Sse9I AATT 4 cut(s) 254, 645, 862, 993
SsiI CCGC 2 cut(s) 133, 385
SspMI CTAG 4 cut(s) 96, 212, 290, 747
StyD4I CCNGG 3 cut(s) 530, 653, 763
StyI CCWWGG 2 cut(s) 173, 501
TaaI ACNGT 4 cut(s) 331, 573, 952, 1016
TaiI ACGT 1 cut(s) 407
TaqI TCGA 2 cut(s) 628, 703
TasI AATT 4 cut(s) 254, 645, 862, 993
TfiI GAWTC 3 cut(s) 461, 625, 908
Tru1I TTAA 2 cut(s) 195, 564
Tru9I TTAA 2 cut(s) 195, 564
TscAI CASTG 3 cut(s) 334, 646, 897
TseFI GTSAC 2 cut(s) 128, 331
TseI GCWGC 2 cut(s) 92, 449
Tsp45I GTSAC 2 cut(s) 128, 331
TspDTI ATGAA 5 cut(s) 179, 267, 686, 712, 726
TspGWI ACGGA 3 cut(s) 194, 258, 311
TspRI CASTG 3 cut(s) 334, 646, 897
Tth111I GACNNNGTC 3 cut(s) 337, 347, 730
VpaK11BI GGWCC 2 cut(s) 35, 1036
XapI RAATTY 3 cut(s) 254, 862, 993
XceI RCATGY 1 cut(s) 890
XmiI GTMKAC 2 cut(s) 114, 877
XspI CTAG 4 cut(s) 96, 212, 290, 747
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.