RLG00000004195

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003376
Physical Location & Seq
Forward (+)
57135494 .. 57137372
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004195

Sequence Viewer

Length: 1053 bp
ATGGCTTCCGAGAGGGAATGTTTTGAACTTTCAGGACCATTGCACCTAACTTCCGTGGACTGGGAAAACACTCATCATCAAAGGTCCGTTGCTGCTAGTTTGGTTCAGGGTGTCTACATTGTTGAGCGCGACCGCCAAGAGAAGCGGGAAGGTTCTGAGGCCCTTGCTCCTCCTTGGTGGGAGTTCTTTCATTTTAAGTTGCTCCGTAAACTAGTGGATGATGTTGGTTTTTCCATCTTTGGTGCCGTTTATGAATTCAAGCCTCCTTCGTCCATTCGTCACCACTCACTGGAGGGAAGACCTTGTTTTGTAATTGCCTTCCGAGGCACCTTGACCAAGTCTGACTCCGTCTCACGAGATCTTGAGTTGGATGTTGAGGTCATCCGCAATGGACTTCACCGATCGTCTCGTTTTGAGATTGCTATACAAGCTGTCAGAAATATGGTTGCTGCTGTAGGGGATTCAAATGTTTGGTTAGCTGGCCATTCCTTAGGGTCATCCATGGCAATGCTTGCTGGGAAACCTATGGCCAAAAATGGCGTTTATCTCAAGTCTTTTCTCTTTAATCCACCATTTGTTTCTGCCCCGATTGAGAGGATTAAGGATAAAAATGTGAAGCATGGGATTCGATTTGCAGGCAGCGTAATTACTGCAGGACTCTCTTTCGCCATGAAGGCTAAACAAAAGCAACATCAGGGAAATCGGTCCGTTGAAGACCCATTCACTACTCTGTCCTCATGGTTCCCTAATCTGTTTGTCAATCCAGGCGATGACATCTGCTCGGAATATATTGGTTATTTTGAGCATAGGAAGAGGATGGTGCAGATTGGAGCTGGGGCCATTGAGAGGTTAGCTACCCAGAATTCGCTCGGAGGTCTACTAATGCATGCAATGGGAAAAGCCTCAGATTCTGAGCCAGCATTGCACCTCATCCCTTCTGCCAACCTGACCGTTAATTTAACCCCTCCGCGGGATCTCAAAGAAGCTCATGGAATTCACCAATGGTGGGCAGACAGTATGCAGTTGAAGTCTGAGGTCCACAGATACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

351

Amino Acids

39.18

Weight (kDa)

8.87

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 242, 326
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 2 cut(s) 114, 877
AccII CGCG 2 cut(s) 129, 970
AciI CCGC 5 cut(s) 133, 145, 385, 968, 970
AclWI GGATC 1 cut(s) 981
AcoI YGGCCR 2 cut(s) 481, 528
AcsI RAATTY 3 cut(s) 254, 862, 993
AfiI CCNNNNNNNGG 6 cut(s) 60, 289, 846, 969, 970, 1006
AgsI TTSAA 5 cut(s) 26, 259, 465, 713, 1027
AhlI ACTAGT 1 cut(s) 211
AjnI CCWGG 1 cut(s) 763
AluBI AGCT 5 cut(s) 431, 479, 833, 854, 986
AluI AGCT 5 cut(s) 431, 479, 833, 854, 986
Alw26I GTCTC 2 cut(s) 355, 411
AlwI GGATC 1 cut(s) 981
AlwNI CAGNNNCTG 1 cut(s) 911
AoxI GGCC 4 cut(s) 159, 481, 528, 837
ApeKI GCWGC 3 cut(s) 92, 449, 639
ApoI RAATTY 3 cut(s) 254, 862, 993
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 6 cut(s) 35, 84, 160, 705, 837, 1036
AsuHPI GGTGA 3 cut(s) 272, 389, 989
AvaII GGWCC 4 cut(s) 35, 84, 705, 1036
AxyI CCTNAGG 1 cut(s) 490
BalI TGGCCA 2 cut(s) 483, 530
BanI GGYRCC 2 cut(s) 242, 326
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 2 cut(s) 304, 720
BbvI GCAGC 3 cut(s) 79, 436, 651
BccI CCATC 2 cut(s) 242, 811
BceAI ACGGC 1 cut(s) 230
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 1 cut(s) 765
BcoDI GTCTC 2 cut(s) 355, 411
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 2 cut(s) 96, 212
BfmI CTRYAG 2 cut(s) 453, 651
BglI GCCNNNNNGGC 1 cut(s) 674
BglII AGATCT 1 cut(s) 358
BisI GCNGC 3 cut(s) 93, 450, 640
BlsI GCNGC 3 cut(s) 94, 451, 641
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 4 cut(s) 35, 84, 705, 1036
BmgT120I GGNCC 6 cut(s) 35, 84, 160, 705, 837, 1036
BmiI GGNNCC 4 cut(s) 244, 328, 743, 838
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 2 cut(s) 304, 720
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 383, 533
BsaJI CCNNGG 5 cut(s) 54, 173, 322, 501, 968
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 289, 846, 969, 970, 1006
Bse1I ACTGG 2 cut(s) 65, 294
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 5 cut(s) 38, 394, 513, 897, 920
BseBI CCWGG 1 cut(s) 765
BseDI CCNNGG 5 cut(s) 54, 173, 322, 501, 968
BseGI GGATG 6 cut(s) 223, 376, 381, 497, 822, 930
BseLI CCNNNNNNNGG 6 cut(s) 60, 289, 846, 969, 970, 1006
BseMI GCAATG 5 cut(s) 38, 394, 513, 897, 920
BseMII CTCAG 4 cut(s) 147, 903, 918, 1023
BseNI ACTGG 2 cut(s) 65, 294
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 3 cut(s) 79, 436, 651
BseYI CCCAGC 2 cut(s) 515, 833
BsgI GTGCAG 1 cut(s) 842
Bsh1236I CGCG 2 cut(s) 129, 970
Bsh1285I CGRYCG 2 cut(s) 133, 404
BshFI GGCC 4 cut(s) 161, 483, 530, 839
BshNI GGYRCC 2 cut(s) 242, 326
BsiEI CGRYCG 2 cut(s) 133, 404
BslI CCNNNNNNNGG 6 cut(s) 60, 289, 846, 969, 970, 1006
BsmAI GTCTC 2 cut(s) 355, 411
BsmBI CGTCTC 2 cut(s) 355, 411
BsnI GGCC 4 cut(s) 161, 483, 530, 839
Bsp143I GATC 3 cut(s) 358, 401, 973
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 5 cut(s) 133, 145, 385, 968, 970
BspANI GGCC 4 cut(s) 161, 483, 530, 839
BspCNI CTCAG 4 cut(s) 148, 904, 917, 1024
BspFNI CGCG 2 cut(s) 129, 970
BspLI GGNNCC 4 cut(s) 244, 328, 743, 838
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 1 cut(s) 981
BspT107I GGYRCC 2 cut(s) 242, 326
BsrDI GCAATG 5 cut(s) 38, 394, 513, 897, 920
BsrI ACTGG 2 cut(s) 65, 294
BssECI CCNNGG 5 cut(s) 54, 173, 322, 501, 968
BssMI GATC 3 cut(s) 358, 401, 973
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 173, 501
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 2 cut(s) 952, 1016
Bst6I CTCTTC 1 cut(s) 806
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 5 cut(s) 481, 513, 637, 888, 918
BstDEI CTNAG 5 cut(s) 156, 490, 904, 912, 1032
BstDSI CCRYGG 3 cut(s) 54, 501, 968
BstF5I GGATG 6 cut(s) 223, 376, 381, 497, 822, 930
BstFNI CGCG 2 cut(s) 129, 970
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 361, 404, 976
BstMAI GTCTC 2 cut(s) 355, 411
BstMBI GATC 3 cut(s) 358, 401, 973
BstMCI CGRYCG 2 cut(s) 133, 404
BstMWI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 2 cut(s) 453, 651
BstUI CGCG 2 cut(s) 129, 970
BstV1I GCAGC 3 cut(s) 79, 436, 651
BstV2I GAAGAC 2 cut(s) 304, 720
BstX2I RGATCY 2 cut(s) 358, 973
BstYI RGATCY 2 cut(s) 358, 973
Bsu36I CCTNAGG 1 cut(s) 490
BsuRI GGCC 4 cut(s) 161, 483, 530, 839
BtgI CCRYGG 3 cut(s) 54, 501, 968
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 6 cut(s) 223, 376, 381, 497, 822, 930
BtsIMutI CAGTG 1 cut(s) 287
Cac8I GCNNGC 5 cut(s) 481, 513, 637, 888, 918
CaiI CAGNNNCTG 1 cut(s) 911
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 6 cut(s) 35, 84, 160, 705, 837, 1036
Cfr42I CCGCGG 1 cut(s) 971
CpoI CGGWCCG 1 cut(s) 705
CspI CGGWCCG 1 cut(s) 705
CviAII CATG 6 cut(s) 502, 620, 670, 738, 887, 989
DdeI CTNAG 5 cut(s) 156, 490, 904, 912, 1032
DpnI GATC 3 cut(s) 360, 403, 975
DpnII GATC 3 cut(s) 358, 401, 973
EaeI YGGCCR 2 cut(s) 481, 528
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
Eco130I CCWWGG 2 cut(s) 173, 501
Eco47I GGWCC 4 cut(s) 35, 84, 705, 1036
Eco81I CCTNAGG 1 cut(s) 490
EcoO109I RGGNCCY 1 cut(s) 160
EcoRI GAATTC 3 cut(s) 254, 862, 993
EcoRII CCWGG 1 cut(s) 763
EcoT14I CCWWGG 2 cut(s) 173, 501
EcoT22I ATGCAT 1 cut(s) 888
ErhI CCWWGG 2 cut(s) 173, 501
Esp3I CGTCTC 2 cut(s) 355, 411
FaeI CATG 6 cut(s) 505, 623, 673, 741, 890, 992
FatI CATG 6 cut(s) 501, 619, 669, 737, 886, 988
FauI CCCGC 2 cut(s) 138, 963
FblI GTMKAC 2 cut(s) 114, 877
Fnu4HI GCNGC 3 cut(s) 93, 450, 640
FokI GGATG 6 cut(s) 230, 368, 383, 484, 829, 917
Fsp4HI GCNGC 3 cut(s) 93, 450, 640
FspBI CTAG 2 cut(s) 96, 212
GlaI GCGC 1 cut(s) 128
GluI GCNGC 3 cut(s) 93, 450, 640
GsaI CCCAGC 2 cut(s) 519, 837
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 4 cut(s) 161, 483, 530, 839
HhaI GCGC 1 cut(s) 129
Hin1II CATG 6 cut(s) 505, 623, 673, 741, 890, 992
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 5 cut(s) 344, 461, 625, 657, 908
HphI GGTGA 3 cut(s) 272, 389, 989
Hpy166II GTNNAC 5 cut(s) 58, 115, 209, 878, 1039
Hpy188III TCNNGA 3 cut(s) 33, 354, 362
Hpy8I GTNNAC 5 cut(s) 58, 115, 209, 878, 1039
HpyAV CCTTC 5 cut(s) 143, 276, 328, 667, 945
HpyCH4III ACNGT 2 cut(s) 952, 1016
HpyCH4V TGCA 8 cut(s) 43, 635, 653, 823, 886, 890, 925, 1021
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
HpyF3I CTNAG 5 cut(s) 156, 490, 904, 912, 1032
Hsp92II CATG 6 cut(s) 505, 623, 673, 741, 890, 992
HspAI GCGC 1 cut(s) 127
KspI CCGCGG 1 cut(s) 971
Kzo9I GATC 3 cut(s) 358, 401, 973
LmnI GCTCC 3 cut(s) 172, 207, 830
Lsp1109I GCAGC 3 cut(s) 79, 436, 651
MaeI CTAG 2 cut(s) 96, 212
MaeIII GTNAC 1 cut(s) 278
MalI GATC 3 cut(s) 360, 403, 975
MboI GATC 3 cut(s) 358, 401, 973
MboII GAAGA 3 cut(s) 309, 725, 823
MflI RGATCY 2 cut(s) 358, 973
MlsI TGGCCA 2 cut(s) 483, 530
MluCI AATT 6 cut(s) 254, 312, 645, 862, 955, 993
MluNI TGGCCA 2 cut(s) 483, 530
MlyI GAGTC 2 cut(s) 338, 651
MmeI TCCRAC 1 cut(s) 348
Mox20I TGGCCA 2 cut(s) 483, 530
Mph1103I ATGCAT 1 cut(s) 888
MscI TGGCCA 2 cut(s) 483, 530
MseI TTAA 5 cut(s) 195, 564, 600, 954, 959
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 2 cut(s) 483, 530
MspA1I CMGCKG 1 cut(s) 970
MspR9I CCNGG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 765
MvnI CGCG 2 cut(s) 129, 970
MwoI GCNNNNNNNGC 4 cut(s) 428, 512, 674, 922
NcoI CCATGG 1 cut(s) 501
NdeII GATC 3 cut(s) 358, 401, 973
NlaIII CATG 6 cut(s) 505, 623, 673, 741, 890, 992
NlaIV GGNNCC 4 cut(s) 244, 328, 743, 838
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 1 cut(s) 890
PaeI GCATGC 1 cut(s) 890
PfeI GAWTC 3 cut(s) 461, 625, 908
PflFI GACNNNGTC 2 cut(s) 337, 347
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 3 cut(s) 94, 451, 641
Ple19I CGATCG 1 cut(s) 404
PleI GAGTC 2 cut(s) 338, 651
PpsI GAGTC 2 cut(s) 338, 651
Psp6I CCWGG 1 cut(s) 763
PspFI CCCAGC 2 cut(s) 515, 833
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 4 cut(s) 244, 328, 743, 838
PspPI GGNCC 6 cut(s) 35, 84, 160, 705, 837, 1036
PstI CTGCAG 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 2 cut(s) 358, 973
PsyI GACNNNGTC 2 cut(s) 337, 347
PvuI CGATCG 1 cut(s) 404
RseI CAYNNNNRTG 1 cut(s) 506
Rsr2I CGGWCCG 1 cut(s) 705
RsrII CGGWCCG 1 cut(s) 705
SacII CCGCGG 1 cut(s) 971
SaqAI TTAA 5 cut(s) 195, 564, 600, 954, 959
SatI GCNGC 3 cut(s) 93, 450, 640
Sau3AI GATC 3 cut(s) 358, 401, 973
Sau96I GGNCC 6 cut(s) 35, 84, 160, 705, 837, 1036
SchI GAGTC 2 cut(s) 338, 651
ScrFI CCNGG 1 cut(s) 765
SfcI CTRYAG 2 cut(s) 453, 651
Sfr303I CCGCGG 1 cut(s) 971
SgrBI CCGCGG 1 cut(s) 971
SinI GGWCC 4 cut(s) 35, 84, 705, 1036
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 2 cut(s) 362, 548
SmoI CTYRAG 2 cut(s) 362, 548
SpeI ACTAGT 1 cut(s) 211
SphI GCATGC 1 cut(s) 890
Sse9I AATT 6 cut(s) 254, 312, 645, 862, 955, 993
SsiI CCGC 5 cut(s) 133, 145, 385, 968, 970
SspMI CTAG 2 cut(s) 96, 212
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 2 cut(s) 173, 501
TaaI ACNGT 2 cut(s) 952, 1016
TaqI TCGA 1 cut(s) 628
TaqII GACCGA 1 cut(s) 693
TasI AATT 6 cut(s) 254, 312, 645, 862, 955, 993
TfiI GAWTC 3 cut(s) 461, 625, 908
Tru1I TTAA 5 cut(s) 195, 564, 600, 954, 959
Tru9I TTAA 5 cut(s) 195, 564, 600, 954, 959
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 92, 449, 639
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 179, 267, 686
TspGWI ACGGA 5 cut(s) 43, 76, 194, 337, 697
TspRI CASTG 1 cut(s) 294
Tth111I GACNNNGTC 2 cut(s) 337, 347
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 4 cut(s) 35, 84, 705, 1036
XapI RAATTY 3 cut(s) 254, 862, 993
XceI RCATGY 1 cut(s) 890
XmiI GTMKAC 2 cut(s) 114, 877
XspI CTAG 2 cut(s) 96, 212
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.