AT4G22190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006877
Physical Location & Seq
Reverse (-)
11741780 .. 11743318
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22190.1

Sequence Viewer

Length: 1164 bp
ATGTGTTATGTACCACAAACTCTGCATATATGGTCACACTGCACCTGCTCATCATCTTTTCTCTCTCTAACGAATTCATTAATTTATTATCTTCTTCTTCTTCTTCGACCTTCCAAAACTCTCTCTCTCTCTCTGTCGTCAACAATGGATAGTCCGACGAGCATACGTAGTAAACCACTACCGGAGACTCTTTCTCCATGCGGTAGTCAACGACGGAGAAGCAGCTGCGACTCTAACCCACCTGAGTTCGAGTTCTGGCGTTTAACTAACTCTTCATTTCCTCAAGCTGATTCAGATCTCCTCTCCGCCGACGAGCTTTTTCACGACGGTGTTCTTCTCCCTCTTGACCTCCTCTCCGTTAAATCAGAGCTTCAGTCCGACCCGAATATCGCAGAATGCGACCCGGATCCATCTCCTTCGACTGGTAGTTTGATTACAGAGCAAAAAAGTGATCTTGAACCCGGTTTAGGATCCGAGTTGACCCGAGAAACAACGGTTTCGAAGCGGTGGAGAGATATTTTCAGGAAGAGCGAAACCAAACCGCCGGGGAAGAAAGAGAAGGTGAAAGAGAATAAGAAGGAGAAGAAGAAAACCGGGTCGGGTCCAAGTTCGGGTTCGGGTTCAGGAGCGGAGCTGAATATCAACATTTGGCCGTTTTCAAGAAGTAGATCCGCTGGTAACAACGTGACCCGACCGAGAATGTCGTTTGGAGCTCCGACGACCCGGAAAGTAAGCAGTGCGCCGTGTTCACGTAGCAACTCCACCGGAGAATCCAAATCGAGGAAGTGGCCGAGTAGTCCCAGTCGTAACGGCGTGCATCTTGGTCGGAATAGTCCGGTTTGGCAAGTCCGGCGTGGAGGAGGAGCTCCGGTTGGGAAAACGATACCGGAACCGATGGGTCGGGTTGTGGGTAAAAGGGAGATTCCCGAGACGCGTAAGGGTAAAACAGTAATTGAGAGCAATAAAGCAAAAGTCTTGAACTTGAACGTGCCTATGTGCATCGGTTATCGGAGCCGGTTAAGCTGCAGAACCGAAGAGAGTAGTGGTGGTGGTAATAGTAACATTGGGAGTGACAACAATAATAATAATAACGCCAACGCTAATAATCCTAATCCTAATGGTTTATTTGGCTTTCGTAATCTCTTCATTAAGAAAGTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

387

Amino Acids

42.33

Weight (kDa)

9.73

Isoelectric Point (pI)

68.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 53
AccBSI CCGCTC 1 cut(s) 629
AccII CGCG 1 cut(s) 934
AciI CCGC 6 cut(s) 201, 306, 505, 542, 629, 672
AclWI GGATC 5 cut(s) 401, 414, 465, 478, 663
AcoI YGGCCR 2 cut(s) 650, 788
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 4 cut(s) 422, 467, 611, 780
AflIII ACRYGT 1 cut(s) 932
AgsI TTSAA 4 cut(s) 458, 660, 979, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 8 cut(s) 225, 287, 316, 370, 634, 713, 866, 1023
AluI AGCT 8 cut(s) 225, 287, 316, 370, 634, 713, 866, 1023
Alw21I GWGCWC 2 cut(s) 715, 868
Alw26I GTCTC 2 cut(s) 179, 923
AlwI GGATC 5 cut(s) 401, 414, 465, 478, 663
Ama87I CYCGRG 2 cut(s) 483, 926
AoxI GGCC 2 cut(s) 650, 788
ApeKI GCWGC 3 cut(s) 222, 225, 1023
ApoI RAATTY 1 cut(s) 73
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AseI ATTAAT 1 cut(s) 80
AspLEI GCGC 1 cut(s) 742
AspS9I GGNCC 1 cut(s) 602
AsuC2I CCSGG 5 cut(s) 404, 462, 546, 595, 724
AsuHPI GGTGA 1 cut(s) 574
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 2 cut(s) 483, 926
AvaII GGWCC 1 cut(s) 602
BamHI GGATCC 2 cut(s) 406, 470
BanII GRGCYC 2 cut(s) 715, 868
Bbv12I GWGCWC 2 cut(s) 715, 868
BbvI GCAGC 3 cut(s) 212, 234, 1010
BccI CCATC 2 cut(s) 418, 889
BceAI ACGGC 3 cut(s) 637, 727, 826
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BcnI CCSGG 5 cut(s) 404, 462, 546, 595, 724
BcoDI GTCTC 2 cut(s) 179, 923
BfmI CTRYAG 1 cut(s) 1024
BfuAI ACCTGC 1 cut(s) 53
BglII AGATCT 1 cut(s) 295
BisI GCNGC 3 cut(s) 223, 226, 1024
BlsI GCNGC 3 cut(s) 224, 227, 1025
Bme1390I CCNGG 5 cut(s) 404, 462, 546, 595, 724
Bme18I GGWCC 1 cut(s) 602
BmeT110I CYCGRG 2 cut(s) 483, 926
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 5 cut(s) 408, 472, 603, 891, 1013
BmrFI CCNGG 5 cut(s) 404, 462, 546, 595, 724
BmrI ACTGGG 1 cut(s) 795
BmsI GCATC 2 cut(s) 826, 1008
BmuI ACTGGG 1 cut(s) 795
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 267
BpuMI CCSGG 5 cut(s) 404, 462, 546, 595, 724
BsaAI YACGTR 2 cut(s) 167, 752
BsaBI GATNNNNATC 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 545
BsaWI WCCGGW 5 cut(s) 181, 764, 835, 868, 886
BsaXI ACNNNNNCTCC 6 cut(s) 178, 208, 338, 368, 855, 885
Bsc4I CCNNNNNNNGG 4 cut(s) 422, 467, 611, 780
Bse118I RCCGGY 1 cut(s) 1014
Bse1I ACTGG 2 cut(s) 427, 801
Bse8I GATNNNNATC 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 545
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 4 cut(s) 422, 467, 611, 780
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 427, 801
BseRI GAGGAG 4 cut(s) 290, 341, 873, 876
BseXI GCAGC 3 cut(s) 212, 234, 1010
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 934
Bsh1285I CGRYCG 1 cut(s) 695
BshFI GGCC 2 cut(s) 652, 790
BsiEI CGRYCG 1 cut(s) 695
BsiHKAI GWGCWC 2 cut(s) 715, 868
BsiHKCI CYCGRG 2 cut(s) 483, 926
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 4 cut(s) 422, 467, 611, 780
BsmAI GTCTC 2 cut(s) 179, 923
BsmBI CGTCTC 1 cut(s) 923
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 2 cut(s) 652, 790
BsoBI CYCGRG 2 cut(s) 483, 926
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 715, 868
Bsp143I GATC 5 cut(s) 295, 406, 451, 470, 668
BspACI CCGC 6 cut(s) 201, 306, 505, 542, 629, 672
BspANI GGCC 2 cut(s) 652, 790
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 934
BspLI GGNNCC 5 cut(s) 408, 472, 603, 891, 1013
BspMAI CTGCAG 1 cut(s) 1028
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 5 cut(s) 401, 414, 465, 478, 663
BspQI GCTCTTC 1 cut(s) 521
BspT104I TTCGAA 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 1014
BsrI ACTGG 2 cut(s) 427, 801
BssAI RCCGGY 1 cut(s) 1014
BssECI CCNNGG 1 cut(s) 545
BssMI GATC 5 cut(s) 295, 406, 451, 470, 668
Bst4CI ACNGT 3 cut(s) 329, 496, 949
Bst6I CTCTTC 4 cut(s) 277, 521, 1029, 1148
BstBAI YACGTR 2 cut(s) 167, 752
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 815
BstDEI CTNAG 1 cut(s) 243
BstFNI CGCG 1 cut(s) 934
BstHHI GCGC 1 cut(s) 742
BstKTI GATC 5 cut(s) 298, 409, 454, 473, 671
BstMAI GTCTC 2 cut(s) 179, 923
BstMBI GATC 5 cut(s) 295, 406, 451, 470, 668
BstMCI CGRYCG 1 cut(s) 695
BstMWI GCNNNNNNNGC 2 cut(s) 850, 1020
BstSCI CCNGG 5 cut(s) 402, 460, 544, 593, 722
BstSFI CTRYAG 1 cut(s) 1024
BstSNI TACGTA 1 cut(s) 167
BstUI CGCG 1 cut(s) 934
BstV1I GCAGC 3 cut(s) 212, 234, 1010
BstX2I RGATCY 4 cut(s) 295, 406, 470, 668
BstYI RGATCY 4 cut(s) 295, 406, 470, 668
BsuRI GGCC 2 cut(s) 652, 790
BtsI GCAGTG 2 cut(s) 37, 742
BtsIMutI CAGTG 2 cut(s) 37, 742
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 815
CfoI GCGC 1 cut(s) 742
Cfr10I RCCGGY 1 cut(s) 1014
Cfr13I GGNCC 1 cut(s) 602
CseI GACGC 1 cut(s) 940
Csp6I GTAC 1 cut(s) 11
CviAII CATG 1 cut(s) 198
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 243
DpnI GATC 5 cut(s) 297, 408, 453, 472, 670
DpnII GATC 5 cut(s) 295, 406, 451, 470, 668
EaeI YGGCCR 2 cut(s) 650, 788
Eam1104I CTCTTC 4 cut(s) 277, 521, 1029, 1148
EarI CTCTTC 4 cut(s) 277, 521, 1029, 1148
EciI GGCGGA 1 cut(s) 295
Ecl136II GAGCTC 2 cut(s) 713, 866
Eco105I TACGTA 1 cut(s) 167
Eco24I GRGCYC 2 cut(s) 715, 868
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 2 cut(s) 713, 866
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 2 cut(s) 483, 926
EcoICRI GAGCTC 2 cut(s) 713, 866
EcoRI GAATTC 1 cut(s) 73
EcoT38I GRGCYC 2 cut(s) 715, 868
Esp3I CGTCTC 1 cut(s) 923
FaeI CATG 1 cut(s) 201
FaiI YATR 7 cut(s) 9, 27, 29, 31, 164, 199, 995
FaqI GGGAC 1 cut(s) 783
FatI CATG 1 cut(s) 197
Fnu4HI GCNGC 3 cut(s) 223, 226, 1024
FriOI GRGCYC 2 cut(s) 715, 868
Fsp4HI GCNGC 3 cut(s) 223, 226, 1024
GlaI GCGC 1 cut(s) 741
GluI GCNGC 3 cut(s) 223, 226, 1024
HaeIII GGCC 2 cut(s) 652, 790
HgaI GACGC 1 cut(s) 940
HhaI GCGC 1 cut(s) 742
Hin1II CATG 1 cut(s) 201
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HincII GTYRAC 3 cut(s) 141, 209, 480
HindII GTYRAC 3 cut(s) 141, 209, 480
HinfI GANTC 5 cut(s) 187, 230, 290, 770, 922
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 5 cut(s) 141, 173, 209, 480, 749
Hpy188I TCNGA 8 cut(s) 156, 295, 367, 379, 475, 717, 828, 1011
Hpy188III TCNNGA 8 cut(s) 323, 344, 455, 523, 624, 660, 926, 976
Hpy8I GTNNAC 5 cut(s) 141, 173, 209, 480, 749
Hpy99I CGWCG 5 cut(s) 160, 216, 314, 329, 721
HpyAV CCTTC 4 cut(s) 120, 426, 553, 571
HpyCH4III ACNGT 3 cut(s) 329, 496, 949
HpyCH4IV ACGT 4 cut(s) 166, 684, 751, 987
HpyCH4V TGCA 5 cut(s) 25, 42, 817, 999, 1026
HpyF10VI GCNNNNNNNGC 2 cut(s) 850, 1020
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 4 cut(s) 166, 684, 751, 987
Hsp92II CATG 1 cut(s) 201
HspAI GCGC 1 cut(s) 740
Kzo9I GATC 5 cut(s) 295, 406, 451, 470, 668
LguI GCTCTTC 1 cut(s) 521
LmnI GCTCC 7 cut(s) 626, 631, 710, 718, 863, 871, 1011
Lsp1109I GCAGC 3 cut(s) 212, 234, 1010
LweI GCATC 2 cut(s) 826, 1008
MaeII ACGT 4 cut(s) 166, 684, 751, 987
MaeIII GTNAC 6 cut(s) 33, 677, 685, 806, 1058, 1070
MalI GATC 5 cut(s) 297, 408, 453, 472, 670
MbiI CCGCTC 1 cut(s) 629
MboI GATC 5 cut(s) 295, 406, 451, 470, 668
MflI RGATCY 4 cut(s) 295, 406, 470, 668
MhlI GDGCHC 2 cut(s) 715, 868
MluCI AATT 3 cut(s) 73, 81, 951
MluI ACGCGT 1 cut(s) 932
MlyI GAGTC 2 cut(s) 181, 224
MmeI TCCRAC 4 cut(s) 179, 402, 740, 806
MnlI CCTC 8 cut(s) 291, 311, 351, 359, 362, 774, 851, 854
MseI TTAA 5 cut(s) 80, 263, 360, 1019, 1149
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 2 cut(s) 225, 674
MspR9I CCNGG 5 cut(s) 404, 462, 546, 595, 724
Mva1269I GAATGC 1 cut(s) 401
MvnI CGCG 1 cut(s) 934
MwoI GCNNNNNNNGC 2 cut(s) 850, 1020
NciI CCSGG 5 cut(s) 404, 462, 546, 595, 724
NdeII GATC 5 cut(s) 295, 406, 451, 470, 668
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 5 cut(s) 408, 472, 603, 891, 1013
NmeAIII GCCGAG 1 cut(s) 816
NmuCI GTSAC 3 cut(s) 33, 685, 1070
NspV TTCGAA 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 327
PaqCI CACCTGC 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 521
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 3 cut(s) 290, 770, 922
PkrI GCNGC 3 cut(s) 224, 227, 1025
PleI GAGTC 2 cut(s) 181, 224
PpsI GAGTC 2 cut(s) 181, 224
Ppu21I YACGTR 2 cut(s) 167, 752
PshBI ATTAAT 1 cut(s) 80
Psp124BI GAGCTC 2 cut(s) 715, 868
PspN4I GGNNCC 5 cut(s) 408, 472, 603, 891, 1013
PspPI GGNCC 1 cut(s) 602
PstI CTGCAG 1 cut(s) 1028
PsuI RGATCY 4 cut(s) 295, 406, 470, 668
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 2 cut(s) 715, 868
SapI GCTCTTC 1 cut(s) 521
SaqAI TTAA 5 cut(s) 80, 263, 360, 1019, 1149
SatI GCNGC 3 cut(s) 223, 226, 1024
Sau3AI GATC 5 cut(s) 295, 406, 451, 470, 668
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 2 cut(s) 181, 224
ScrFI CCNGG 5 cut(s) 404, 462, 546, 595, 724
SduI GDGCHC 2 cut(s) 715, 868
SfaNI GCATC 2 cut(s) 826, 1008
SfcI CTRYAG 1 cut(s) 1024
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
SnaBI TACGTA 1 cut(s) 167
Sse9I AATT 3 cut(s) 73, 81, 951
SsiI CCGC 6 cut(s) 201, 306, 505, 542, 629, 672
SstI GAGCTC 2 cut(s) 715, 868
StyD4I CCNGG 5 cut(s) 402, 460, 544, 593, 722
TaaI ACNGT 3 cut(s) 329, 496, 949
TaiI ACGT 4 cut(s) 169, 687, 754, 990
TaqI TCGA 5 cut(s) 106, 249, 419, 500, 779
TaqII GACCGA 1 cut(s) 709
TasI AATT 3 cut(s) 73, 81, 951
TfiI GAWTC 3 cut(s) 290, 770, 922
Tru1I TTAA 5 cut(s) 80, 263, 360, 1019, 1149
Tru9I TTAA 5 cut(s) 80, 263, 360, 1019, 1149
TscAI CASTG 2 cut(s) 44, 742
TseFI GTSAC 3 cut(s) 33, 685, 1070
TseI GCWGC 3 cut(s) 222, 225, 1023
Tsp45I GTSAC 3 cut(s) 33, 685, 1070
TspDTI ATGAA 3 cut(s) 66, 264, 1135
TspGWI ACGGA 2 cut(s) 229, 346
TspRI CASTG 2 cut(s) 44, 742
VpaK11BI GGWCC 1 cut(s) 602
VspI ATTAAT 1 cut(s) 80
XapI RAATTY 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.