Rmu_co8161534.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8161534.1
OG0006877
Physical Location & Seq
Forward (+)
1 .. 654
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8161534.1_g000001.1.cds

Sequence Viewer

Length: 654 bp
gagaaggagaaggagaaggagaagaagaaggagcggaagagcggcggagcagccaccggagcgagctcggcggagctgaacatcaatctgtggccgttttcgcggagcagatccgccggaaatgcttgtacccgacccaagttcggggctccgggaacccggaaggtgaacagcgcgccgtgttcgaggagcaactcgacgggcgagtccaagtcaacgaggaagtggccggcgagtccgggtcgggccggagtccacttgggtcggagcagcccggtttggcaggtccggcgtggagcctcaaccctgggtaaaacctccgaaacccaagtcaagaatgctgaaaagagcaccaaaaaagaagcacccgaaactcgccggagtaaaaccaccgcatccggcggcggtcgcggcggaggaggtggtgcaaaacccagagtcttgaacttgaatgttccgacgtgtatcgggtacagaagccatttgagttgtagaagtgacgtcatgaatagtgctgtaggaataggcggcagtagtggtagcaacaaccaccgtggcgccgccgctggcggtggcggagatagtactaacgctgctggggctggtagtaatctttttaatctgcgcagccttttctctaagaaggtgtattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

22.42

Weight (kDa)

10.91

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AatII GACGTC 1 cut(s) 504
Acc16I TGCGCA 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 557
AccBSI CCGCTC 2 cut(s) 34, 42
AccII CGCG 3 cut(s) 103, 176, 411
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 2 cut(s) 92, 227
AcyI GRCGYC 2 cut(s) 501, 558
AfaI GTAC 3 cut(s) 130, 473, 586
AfiI CCNNNNNNNGG 2 cut(s) 143, 144
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 2 cut(s) 445, 451
AjiI CACGTC 1 cut(s) 462
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 2 cut(s) 66, 76
AluI AGCT 2 cut(s) 66, 76
Alw21I GWGCWC 2 cut(s) 68, 353
AlwI GGATC 1 cut(s) 105
AoxI GGCC 3 cut(s) 92, 227, 246
ApeKI GCWGC 4 cut(s) 50, 270, 593, 627
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AspLEI GCGC 4 cut(s) 176, 178, 560, 627
AspS9I GGNCC 2 cut(s) 246, 286
AsuC2I CCSGG 4 cut(s) 153, 160, 240, 275
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 68, 151
Bbv12I GWGCWC 2 cut(s) 68, 353
BbvI GCAGC 4 cut(s) 62, 282, 580, 639
BceAI ACGGC 2 cut(s) 79, 163
BciT130I CCWGG 1 cut(s) 308
BcnI CCSGG 4 cut(s) 153, 160, 240, 275
BfmI CTRYAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 274
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 5 cut(s) 153, 160, 240, 275, 308
Bme18I GGWCC 1 cut(s) 286
BmgBI CACGTC 1 cut(s) 462
BmgT120I GGNCC 2 cut(s) 246, 286
BmiI GGNNCC 4 cut(s) 150, 157, 298, 559
BmrFI CCNGG 5 cut(s) 153, 160, 240, 275, 308
BmsI GCATC 1 cut(s) 404
BpuMI CCSGG 4 cut(s) 153, 160, 240, 275
BsaHI GRCGYC 2 cut(s) 501, 558
BsaJI CCNNGG 3 cut(s) 306, 307, 553
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 144
Bse118I RCCGGY 1 cut(s) 229
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 3 cut(s) 306, 307, 553
BseGI GGATG 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 143, 144
BsePI GCGCGC 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 202, 432
BseXI GCAGC 4 cut(s) 62, 282, 580, 639
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 3 cut(s) 103, 176, 411
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 3 cut(s) 94, 229, 248
BshNI GGYRCC 1 cut(s) 557
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 68, 353
BslI CCNNNNNNNGG 2 cut(s) 143, 144
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 3 cut(s) 94, 229, 248
Bsp1286I GDGCHC 3 cut(s) 68, 151, 353
Bsp143I GATC 1 cut(s) 110
BspANI GGCC 3 cut(s) 94, 229, 248
BspFNI CGCG 3 cut(s) 103, 176, 411
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 4 cut(s) 150, 157, 298, 559
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 1 cut(s) 105
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 557
BsrBI CCGCTC 2 cut(s) 34, 42
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 306, 307, 553
BssHII GCGCGC 1 cut(s) 174
BssMI GATC 1 cut(s) 110
BssNI GRCGYC 2 cut(s) 501, 558
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 2 cut(s) 501, 558
BstC8I GCNNGC 4 cut(s) 64, 176, 231, 568
BstDEI CTNAG 1 cut(s) 639
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 1 cut(s) 395
BstFNI CGCG 3 cut(s) 103, 176, 411
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 4 cut(s) 176, 178, 560, 627
BstKTI GATC 1 cut(s) 113
BstMBI GATC 1 cut(s) 110
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 8 cut(s) 59, 68, 100, 122, 289, 408, 411, 599
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 5 cut(s) 151, 158, 238, 273, 306
BstSFI CTRYAG 1 cut(s) 516
BstUI CGCG 3 cut(s) 103, 176, 411
BstV1I GCAGC 4 cut(s) 62, 282, 580, 639
BstX2I RGATCY 1 cut(s) 110
BstYI RGATCY 1 cut(s) 110
BsuRI GGCC 3 cut(s) 94, 229, 248
BtgI CCRYGG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 462
BtsCI GGATG 1 cut(s) 395
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 4 cut(s) 64, 176, 231, 568
CciI TCATGA 1 cut(s) 504
CfoI GCGC 4 cut(s) 176, 178, 560, 627
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 246, 286
Csp6I GTAC 3 cut(s) 129, 472, 585
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 1 cut(s) 505
CviQI GTAC 3 cut(s) 129, 472, 585
DdeI CTNAG 1 cut(s) 639
DinI GGCGCC 1 cut(s) 559
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 2 cut(s) 92, 227
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 5 cut(s) 60, 86, 103, 429, 591
Ecl136II GAGCTC 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 68, 151
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 306
EcoT38I GRGCYC 2 cut(s) 68, 151
EgeI GGCGCC 1 cut(s) 559
EheI GGCGCC 1 cut(s) 559
FaeI CATG 1 cut(s) 508
FaiI YATR 1 cut(s) 506
FatI CATG 1 cut(s) 504
FokI GGATG 1 cut(s) 382
FriOI GRGCYC 2 cut(s) 68, 151
FspI TGCGCA 1 cut(s) 626
GlaI GCGC 4 cut(s) 175, 177, 559, 626
GsaI CCCAGC 1 cut(s) 600
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 3 cut(s) 94, 229, 248
HhaI GCGC 4 cut(s) 176, 178, 560, 627
Hin1I GRCGYC 2 cut(s) 501, 558
Hin1II CATG 1 cut(s) 508
Hin6I GCGC 4 cut(s) 174, 176, 558, 625
HinP1I GCGC 4 cut(s) 174, 176, 558, 625
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HinfI GANTC 4 cut(s) 206, 235, 252, 438
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 3 cut(s) 169, 216, 256
Hpy188I TCNGA 3 cut(s) 267, 322, 459
Hpy188III TCNNGA 3 cut(s) 334, 442, 505
Hpy8I GTNNAC 3 cut(s) 169, 216, 256
Hpy99I CGWCG 2 cut(s) 202, 463
HpyAV CCTTC 5 cut(s) 4, 10, 22, 157, 637
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 2 cut(s) 461, 501
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 68, 100, 122, 289, 408, 411, 599
HpyF3I CTNAG 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 461, 501
Hsp92I GRCGYC 2 cut(s) 501, 558
Hsp92II CATG 1 cut(s) 508
HspAI GCGC 4 cut(s) 174, 176, 558, 625
KasI GGCGCC 1 cut(s) 557
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Kzo9I GATC 1 cut(s) 110
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 9 cut(s) 31, 47, 59, 73, 105, 154, 189, 267, 296
Lsp1109I GCAGC 4 cut(s) 62, 282, 580, 639
LweI GCATC 1 cut(s) 404
MaeII ACGT 2 cut(s) 461, 501
MaeIII GTNAC 1 cut(s) 497
MalI GATC 1 cut(s) 112
MbiI CCGCTC 2 cut(s) 34, 42
MboI GATC 1 cut(s) 110
MboII GAAGA 3 cut(s) 34, 37, 49
MflI RGATCY 1 cut(s) 110
MhlI GDGCHC 3 cut(s) 68, 151, 353
Mly113I GGCGCC 1 cut(s) 558
MlyI GAGTC 4 cut(s) 215, 244, 261, 447
MmeI TCCRAC 2 cut(s) 245, 482
MnlI CCTC 6 cut(s) 180, 213, 310, 328, 410, 413
MroNI GCCGGC 1 cut(s) 229
MseI TTAA 2 cut(s) 618, 652
MspA1I CMGCKG 1 cut(s) 566
MspR9I CCNGG 5 cut(s) 153, 160, 240, 275, 308
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 3 cut(s) 103, 176, 411
MwoI GCNNNNNNNGC 8 cut(s) 59, 68, 100, 122, 289, 408, 411, 599
NaeI GCCGGC 1 cut(s) 231
NarI GGCGCC 1 cut(s) 558
NciI CCSGG 4 cut(s) 153, 160, 240, 275
NdeII GATC 1 cut(s) 110
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 508
NlaIV GGNNCC 4 cut(s) 150, 157, 298, 559
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 497
NsbI TGCGCA 1 cut(s) 626
PagI TCATGA 1 cut(s) 504
PasI CCCWGGG 1 cut(s) 307
PauI GCGCGC 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 32
PctI GAATGC 1 cut(s) 343
PdiI GCCGGC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 151
PleI GAGTC 4 cut(s) 214, 243, 260, 446
PluTI GGCGCC 1 cut(s) 561
PpsI GAGTC 4 cut(s) 214, 243, 260, 446
Psp124BI GAGCTC 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 4 cut(s) 150, 157, 298, 559
PspPI GGNCC 2 cut(s) 246, 286
PsuI RGATCY 1 cut(s) 110
PteI GCGCGC 1 cut(s) 174
RsaI GTAC 3 cut(s) 130, 473, 586
RsaNI GTAC 3 cut(s) 129, 472, 585
SacI GAGCTC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 618, 652
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 2 cut(s) 246, 286
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 4 cut(s) 215, 244, 261, 447
ScrFI CCNGG 5 cut(s) 153, 160, 240, 275, 308
SduI GDGCHC 3 cut(s) 68, 151, 353
SetI ASST 9 cut(s) 68, 78, 168, 288, 320, 424, 464, 504, 648
SfaNI GCATC 1 cut(s) 404
SfcI CTRYAG 1 cut(s) 516
SfoI GGCGCC 1 cut(s) 559
SinI GGWCC 1 cut(s) 286
SspDI GGCGCC 1 cut(s) 557
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 5 cut(s) 151, 158, 238, 273, 306
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 2 cut(s) 464, 504
TaqI TCGA 2 cut(s) 185, 197
TatI WGTACW 1 cut(s) 584
TauI GCSGC 6 cut(s) 45, 405, 414, 531, 563, 566
Tru1I TTAA 2 cut(s) 618, 652
Tru9I TTAA 2 cut(s) 618, 652
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 4 cut(s) 50, 270, 593, 627
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 286
ZraI GACGTC 1 cut(s) 502
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.