pycom10g27680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006877
Physical Location & Seq
Reverse (-)
28565272 .. 28566351
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27680.1

Sequence Viewer

Length: 1080 bp
ATGGAGCACAGCCCCAAATTCAAACCCCTCCTTTCACAGAGCAGCAGTCGAAGAAGGCGAACCAGCACCGATTCCAACTCGCCCGAGTTCGAGTTCTGGAGAAACCCCTCTTTCCCTCAGCCCAATCTCCTCTCCGCCGACGAGCTGTTCGTCGATGGCGTCCTCCTCCCTCTCCACCTCCTCCCCCCGCCTCTCCCTAACAACTCCGACCCACCCGACCCATATCCAAGATCTTCCGATCCAAATACACAACCCATCTCGCAACCACCTGTGCCTGACCCAGAACCTTTATTGGAACCGGACCCGGAAGCAGAACCGGGTCCAGGACCCGAGTTGTCAGCTGCTCCGGTAACCGCATCTAAGCGTTGGAGAGACATTTTCAAGAAGGGGGAGAAGAAATCAGTCAAAGGCGACGAGATAGATAAAGACAAAGACAAGAAGAAAGAGCAGAAGGGCGGCAGGAGCGGAGCGAGCTCGGCCGAGCTGAACATTAATATATGGCCATTTTCGCGCAGTAGATCCGCCGGAAACGCGTATACCCGACCCAAACCATTCGGAGCTCAGGGGACCCGAAAGGTGAACAGTGCGCCGTGTTCAAGGAGCAACTCAACGGGGGATTCGAAGTCTAGAAAGTGGCCGCCTGCGAGTCCGGGTCGGCCCGGAGTCCATTTGGGTCGGAGCAGTCCTGTTTGGCAGGTCCGGCGTGGAGCATCCGCGGCTGCAAAGAGAAACTCCGAACCACAAGCTCGAAATGCTGAAAAAGGTACAAAACAAGAAGTACCCGAGATTCGCCGGAGCAAAAAAACGGCCGGATCAGTTGTCGCCGGCGGCAGTGGCTCGAAAGCTAGAGTCTTGAATTTGAATGTTCCGATGTGTATTGGGTACAGAAGCCATTTGAGCTGTAGAAGTGAGAATAGTGCTGTAGGAGTCGGGGTCGGCGGTGGCAGCAGCAGCATCAACCTTCGTGGTGGTGTTAGCGGCGGCGTTAACGGTGGAGGTGGCAATGGCATCAGTGGGAGTGGTGGGGCTGGTGGTAATCTTTTTAATCTGCGCAGCCTTTTCACTAAGAAGGTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

38.12

Weight (kDa)

10.16

Isoelectric Point (pI)

68.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1052
Acc36I ACCTGC 1 cut(s) 685
AccBSI CCGCTC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 536
AccII CGCG 3 cut(s) 511, 533, 716
AclWI GGATC 3 cut(s) 233, 513, 820
AcoI YGGCCR 4 cut(s) 477, 500, 635, 807
AcsI RAATTY 2 cut(s) 17, 856
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 3 cut(s) 766, 780, 884
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 5 cut(s) 22, 382, 597, 856, 862
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 8 cut(s) 145, 341, 474, 484, 560, 746, 845, 900
AluI AGCT 8 cut(s) 145, 341, 474, 484, 560, 746, 845, 900
Alw21I GWGCWC 3 cut(s) 9, 476, 562
Alw26I GTCTC 1 cut(s) 366
AlwI GGATC 3 cut(s) 233, 513, 820
Ama87I CYCGRG 3 cut(s) 83, 329, 782
AoxI GGCC 5 cut(s) 477, 500, 635, 656, 807
ApeKI GCWGC 7 cut(s) 42, 341, 719, 945, 948, 951, 1053
ApoI RAATTY 2 cut(s) 17, 856
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 3 cut(s) 513, 589, 1053
AspS9I GGNCC 6 cut(s) 301, 320, 326, 567, 657, 697
AsuC2I CCSGG 4 cut(s) 305, 318, 651, 660
AsuHPI GGTGA 1 cut(s) 589
AsuII TTCGAA 1 cut(s) 620
AvaI CYCGRG 3 cut(s) 83, 329, 782
AvaII GGWCC 5 cut(s) 301, 320, 326, 567, 697
BalI TGGCCA 1 cut(s) 502
BanII GRGCYC 2 cut(s) 476, 562
Bbv12I GWGCWC 3 cut(s) 9, 476, 562
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 7 cut(s) 54, 328, 706, 957, 960, 963, 1065
BccI CCATC 2 cut(s) 149, 263
BceAI ACGGC 2 cut(s) 574, 822
BciT130I CCWGG 1 cut(s) 324
BcnI CCSGG 4 cut(s) 305, 318, 651, 660
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 2 cut(s) 627, 846
BfmI CTRYAG 2 cut(s) 901, 921
BfuAI ACCTGC 1 cut(s) 685
BglII AGATCT 1 cut(s) 230
Bme1390I CCNGG 5 cut(s) 305, 318, 324, 651, 660
Bme18I GGWCC 5 cut(s) 301, 320, 326, 567, 697
BmeT110I CYCGRG 3 cut(s) 83, 329, 782
BmgT120I GGNCC 6 cut(s) 301, 320, 326, 567, 657, 697
BmiI GGNNCC 6 cut(s) 297, 303, 321, 328, 568, 569
BmrFI CCNGG 5 cut(s) 305, 318, 324, 651, 660
BmsI GCATC 4 cut(s) 365, 719, 963, 1017
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 2 cut(s) 117, 561
Bpu14I TTCGAA 1 cut(s) 620
BpuMI CCSGG 4 cut(s) 305, 318, 651, 660
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 714
BsaWI WCCGGW 2 cut(s) 298, 346
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 1 cut(s) 824
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 714
BseGI GGATG 1 cut(s) 710
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 2 cut(s) 131, 575
BseRI GAGGAG 3 cut(s) 119, 155, 170
BseX3I CGGCCG 2 cut(s) 477, 807
BseXI GCAGC 7 cut(s) 54, 328, 706, 957, 960, 963, 1065
Bsh1236I CGCG 3 cut(s) 511, 533, 716
Bsh1285I CGRYCG 2 cut(s) 480, 810
BshFI GGCC 5 cut(s) 479, 502, 637, 658, 809
BsiEI CGRYCG 2 cut(s) 480, 810
BsiHKAI GWGCWC 3 cut(s) 9, 476, 562
BsiHKCI CYCGRG 3 cut(s) 83, 329, 782
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 366
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 5 cut(s) 479, 502, 637, 658, 809
BsoBI CYCGRG 3 cut(s) 83, 329, 782
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 3 cut(s) 9, 476, 562
Bsp143I GATC 4 cut(s) 230, 238, 518, 812
BspANI GGCC 5 cut(s) 479, 502, 637, 658, 809
BspCNI CTCAG 2 cut(s) 130, 574
BspFNI CGCG 3 cut(s) 511, 533, 716
BspLI GGNNCC 6 cut(s) 297, 303, 321, 328, 568, 569
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 3 cut(s) 233, 513, 820
BspT104I TTCGAA 1 cut(s) 620
BsrBI CCGCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 1 cut(s) 824
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 1 cut(s) 714
BssMI GATC 4 cut(s) 230, 238, 518, 812
BssNAI GTATAC 1 cut(s) 537
BssNI GRCGYC 1 cut(s) 159
Bst1107I GTATAC 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 584, 992
BstACI GRCGYC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 472, 642, 826
BstDEI CTNAG 4 cut(s) 117, 360, 561, 1065
BstDSI CCRYGG 1 cut(s) 714
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 1 cut(s) 710
BstFNI CGCG 3 cut(s) 511, 533, 716
BstHHI GCGC 3 cut(s) 513, 589, 1053
BstKTI GATC 4 cut(s) 233, 241, 521, 815
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 4 cut(s) 230, 238, 518, 812
BstMCI CGRYCG 2 cut(s) 480, 810
BstNI CCWGG 1 cut(s) 324
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 5 cut(s) 303, 316, 322, 649, 658
BstSFI CTRYAG 2 cut(s) 901, 921
BstUI CGCG 3 cut(s) 511, 533, 716
BstV1I GCAGC 7 cut(s) 54, 328, 706, 957, 960, 963, 1065
BstX2I RGATCY 2 cut(s) 230, 518
BstYI RGATCY 2 cut(s) 230, 518
BstZ17I GTATAC 1 cut(s) 537
BstZI CGGCCG 2 cut(s) 477, 807
BsuRI GGCC 5 cut(s) 479, 502, 637, 658, 809
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 1 cut(s) 710
BtsI GCAGTG 1 cut(s) 838
BtsIMutI CAGTG 3 cut(s) 589, 838, 1018
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 3 cut(s) 472, 642, 826
CfoI GCGC 3 cut(s) 513, 589, 1053
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 6 cut(s) 301, 320, 326, 567, 657, 697
Cfr42I CCGCGG 1 cut(s) 717
CseI GACGC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 765, 779, 883
CspCI CAANNNNNGTGG 2 cut(s) 946, 981
CviQI GTAC 3 cut(s) 765, 779, 883
DdeI CTNAG 4 cut(s) 117, 360, 561, 1065
DpnI GATC 4 cut(s) 232, 240, 520, 814
DpnII GATC 4 cut(s) 230, 238, 518, 812
EaeI YGGCCR 4 cut(s) 477, 500, 635, 807
EagI CGGCCG 2 cut(s) 477, 807
EciI GGCGGA 2 cut(s) 124, 511
Ecl136II GAGCTC 2 cut(s) 474, 560
EclXI CGGCCG 2 cut(s) 477, 807
Eco24I GRGCYC 2 cut(s) 476, 562
Eco47I GGWCC 5 cut(s) 301, 320, 326, 567, 697
Eco52I CGGCCG 2 cut(s) 477, 807
Eco53kI GAGCTC 2 cut(s) 474, 560
Eco88I CYCGRG 3 cut(s) 83, 329, 782
Eco91I GGTNACC 1 cut(s) 349
EcoICRI GAGCTC 2 cut(s) 474, 560
EcoO109I RGGNCCY 2 cut(s) 326, 567
EcoO65I GGTNACC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 476, 562
FaiI YATR 4 cut(s) 223, 497, 499, 537
FaqI GGGAC 1 cut(s) 580
FauI CCCGC 1 cut(s) 195
FblI GTMKAC 1 cut(s) 536
FokI GGATG 1 cut(s) 697
FriOI GRGCYC 2 cut(s) 476, 562
FspBI CTAG 2 cut(s) 627, 846
FspI TGCGCA 1 cut(s) 1052
GlaI GCGC 3 cut(s) 512, 588, 1052
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 5 cut(s) 479, 502, 637, 658, 809
HgaI GACGC 1 cut(s) 148
HhaI GCGC 3 cut(s) 513, 589, 1053
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 3 cut(s) 511, 587, 1051
HinP1I GCGC 3 cut(s) 511, 587, 1051
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HinfI GANTC 7 cut(s) 71, 617, 646, 663, 787, 849, 927
HpaI GTTAAC 1 cut(s) 988
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 3 cut(s) 537, 580, 988
Hpy188I TCNGA 6 cut(s) 208, 238, 557, 678, 736, 870
Hpy188III TCNNGA 4 cut(s) 97, 382, 627, 853
Hpy8I GTNNAC 3 cut(s) 537, 580, 988
Hpy99I CGWCG 3 cut(s) 143, 155, 416
HpyAV CCTTC 5 cut(s) 48, 379, 445, 971, 1063
HpyCH4III ACNGT 2 cut(s) 584, 992
HpyCH4V TGCA 1 cut(s) 722
HpyF3I CTNAG 4 cut(s) 117, 360, 561, 1065
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 3 cut(s) 511, 587, 1051
KflI GGGWCCC 1 cut(s) 567
KroI GCCGGC 1 cut(s) 824
KroNI GCCGGC 1 cut(s) 826
KspAI GTTAAC 1 cut(s) 988
KspI CCGCGG 1 cut(s) 717
Kzo9I GATC 4 cut(s) 230, 238, 518, 812
LmnI GCTCC 9 cut(s) 4, 349, 462, 467, 557, 600, 678, 707, 795
Lsp1109I GCAGC 7 cut(s) 54, 328, 706, 957, 960, 963, 1065
LweI GCATC 4 cut(s) 365, 719, 963, 1017
MaeI CTAG 2 cut(s) 627, 846
MaeIII GTNAC 1 cut(s) 349
MalI GATC 4 cut(s) 232, 240, 520, 814
MbiI CCGCTC 1 cut(s) 465
MboI GATC 4 cut(s) 230, 238, 518, 812
MboII GAAGA 4 cut(s) 63, 225, 406, 451
MflI RGATCY 2 cut(s) 230, 518
MhlI GDGCHC 3 cut(s) 9, 476, 562
MlsI TGGCCA 1 cut(s) 502
MluCI AATT 2 cut(s) 17, 856
MluI ACGCGT 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 502
MlyI GAGTC 4 cut(s) 655, 672, 858, 936
MmeI TCCRAC 4 cut(s) 99, 231, 347, 656
Mox20I TGGCCA 1 cut(s) 502
MreI CGCCGGCG 1 cut(s) 824
MroNI GCCGGC 1 cut(s) 824
MscI TGGCCA 1 cut(s) 502
MseI TTAA 4 cut(s) 492, 987, 1044, 1078
Msp20I TGGCCA 1 cut(s) 502
MspA1I CMGCKG 2 cut(s) 341, 716
MspR9I CCNGG 5 cut(s) 305, 318, 324, 651, 660
MvaI CCWGG 1 cut(s) 324
MvnI CGCG 3 cut(s) 511, 533, 716
NaeI GCCGGC 1 cut(s) 826
NciI CCSGG 4 cut(s) 305, 318, 651, 660
NdeII GATC 4 cut(s) 230, 238, 518, 812
NgoMIV GCCGGC 1 cut(s) 824
NlaIV GGNNCC 6 cut(s) 297, 303, 321, 328, 568, 569
NmeAIII GCCGAG 2 cut(s) 455, 505
NsbI TGCGCA 1 cut(s) 1052
NspV TTCGAA 1 cut(s) 620
PcsI WCGNNNNNNNCGW 4 cut(s) 55, 147, 156, 617
PdiI GCCGGC 1 cut(s) 826
PfeI GAWTC 3 cut(s) 71, 617, 787
PfoI TCCNGGA 1 cut(s) 322
PleI GAGTC 4 cut(s) 654, 671, 857, 935
PpsI GAGTC 4 cut(s) 654, 671, 857, 935
PpuMI RGGWCCY 2 cut(s) 326, 567
PshBI ATTAAT 1 cut(s) 492
Psp124BI GAGCTC 2 cut(s) 476, 562
Psp5II RGGWCCY 2 cut(s) 326, 567
Psp6I CCWGG 1 cut(s) 322
PspEI GGTNACC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 6 cut(s) 297, 303, 321, 328, 568, 569
PspPI GGNCC 6 cut(s) 301, 320, 326, 567, 657, 697
PspPPI RGGWCCY 2 cut(s) 326, 567
PsuI RGATCY 2 cut(s) 230, 518
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 3 cut(s) 766, 780, 884
RsaNI GTAC 3 cut(s) 765, 779, 883
SacI GAGCTC 2 cut(s) 476, 562
SacII CCGCGG 1 cut(s) 717
SaqAI TTAA 4 cut(s) 492, 987, 1044, 1078
Sau3AI GATC 4 cut(s) 230, 238, 518, 812
Sau96I GGNCC 6 cut(s) 301, 320, 326, 567, 657, 697
SchI GAGTC 4 cut(s) 655, 672, 858, 936
ScrFI CCNGG 5 cut(s) 305, 318, 324, 651, 660
SduI GDGCHC 3 cut(s) 9, 476, 562
SfaNI GCATC 4 cut(s) 365, 719, 963, 1017
SfcI CTRYAG 2 cut(s) 901, 921
Sfr303I CCGCGG 1 cut(s) 717
SfuI TTCGAA 1 cut(s) 620
SgrAI CRCCGGYG 1 cut(s) 824
SgrBI CCGCGG 1 cut(s) 717
SinI GGWCC 5 cut(s) 301, 320, 326, 567, 697
Sse9I AATT 2 cut(s) 17, 856
SspMI CTAG 2 cut(s) 627, 846
SstI GAGCTC 2 cut(s) 476, 562
StyD4I CCNGG 5 cut(s) 303, 316, 322, 649, 658
TaaI ACNGT 2 cut(s) 584, 992
TaqI TCGA 6 cut(s) 49, 90, 153, 620, 748, 839
TasI AATT 2 cut(s) 17, 856
TauI GCSGC 6 cut(s) 459, 640, 719, 831, 981, 984
TfiI GAWTC 3 cut(s) 71, 617, 787
Tru1I TTAA 4 cut(s) 492, 987, 1044, 1078
Tru9I TTAA 4 cut(s) 492, 987, 1044, 1078
TscAI CASTG 3 cut(s) 589, 838, 1018
TseI GCWGC 7 cut(s) 42, 341, 719, 945, 948, 951, 1053
TspRI CASTG 3 cut(s) 589, 838, 1018
VpaK11BI GGWCC 5 cut(s) 301, 320, 326, 567, 697
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 2 cut(s) 17, 856
XbaI TCTAGA 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 2 cut(s) 627, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.