Rh5DG017300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006877
Physical Location & Seq
Forward (+)
1328938 .. 1331446
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG017300.1

Sequence Viewer

Length: 1011 bp
ATGGCAGATGGCTCCAGATTCAAACCCTACAGTCGCAACAGTACTCAAAGAAGCCGAACCAGCACCGACTCCAGCTCCCCGGAATTCGACTTCTGGAGGCTCCAAAACCCGTCTTGCCCGCAACCCGATCTCATCTCTGCCGATGAACTATTCGTCGACGGTGTCATTCTCCCTCTCCGCCTCGTCCCCGCCCCCAAAAACCCGCCCGACCCGAACCCGCGACCCGACTCGGAAGAGATTTCTGCTCCGGACCCAGAACCGGAACCCGGCCCGGAAGCCCAGGTGTCGACCGGAGCTCTGGTGCTGACCGCATCGAAGCGGTGGAGGGACATGTTCAAGAAGAAGAGTGAGGGTGGTGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGGAGCGGAAGAGCGGCGGAGCAGCCACCGGAGCCAGCTCGGCGGAGCTGAACATCAATCTGTGGCCGTTTTCGCGGAGCAGATCCGCCGGAAATGCTTGTACCCGACCCAAATTCGGGGCTCCGGGAACCCGGAAGGTGAACAGCGCGCCGTGTTCGAGGAGCAACTCGACGGGCGAGTCCAAGTCAACGAGGAAGTGGCCGGCGAGTCCGGGTCGGGCCGGAGTCCACTTGGGTCGGAGCAGCCCGGTTTGGCAGGTCCGGCGTGGAGCCTCAACCCTGGGTAAAACCTCCGAAACCCAAGTCAGGAATGCTGAAAAGAGCACCAAAAAAGAAGCACCCGAAACTCGCCGGAGTAAAACCACCGCATCAGGCGGCGGTCGCGGCGGCGGAGGTGGTGCAAAACCCAGAGTCTTGAACTTGAATGTTCCGACGTGTATCGGGTACAGAAGTCATTTGAGTTGTAGAAGTGACGTCATGAATAGTGCTGTAGGAATCGGCGGCAGTAGTGGTAGCAACAACCACCGTGGCGCCGCCGCTGGCGGTGGCGGAGATAGTAGTAACGCTGCTGGGGCTGGTAGTAATCTTTTTAATCTGCGCAGCCTTTTCTCTAAGAAGGTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

336

Amino Acids

35.54

Weight (kDa)

10.18

Isoelectric Point (pI)

61.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 562
AatII GACGTC 1 cut(s) 861
Acc16I TGCGCA 1 cut(s) 983
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 914
AccBSI CCGCTC 2 cut(s) 391, 399
AccI GTMKAC 2 cut(s) 156, 287
AccII CGCG 4 cut(s) 220, 460, 533, 768
AccIII TCCGGA 1 cut(s) 247
AclWI GGATC 1 cut(s) 462
AcoI YGGCCR 2 cut(s) 449, 584
AcsI RAATTY 2 cut(s) 83, 497
AcyI GRCGYC 2 cut(s) 858, 915
AfaI GTAC 3 cut(s) 43, 487, 830
AfiI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
AflIII ACRYGT 2 cut(s) 330, 818
AgsI TTSAA 4 cut(s) 22, 337, 802, 808
AjiI CACGTC 1 cut(s) 819
AjnI CCWGG 2 cut(s) 279, 663
AluBI AGCT 4 cut(s) 75, 296, 423, 433
AluI AGCT 4 cut(s) 75, 296, 423, 433
Alw21I GWGCWC 2 cut(s) 298, 710
AlwI GGATC 1 cut(s) 462
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 4 cut(s) 268, 449, 584, 603
ApeKI GCWGC 4 cut(s) 407, 627, 950, 984
ApoI RAATTY 2 cut(s) 83, 497
ArsI GACNNNNNNTTYG 2 cut(s) 672, 704
AspLEI GCGC 4 cut(s) 533, 535, 917, 984
AspS9I GGNCC 4 cut(s) 250, 269, 603, 643
AsuC2I CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
AsuHPI GGTGA 2 cut(s) 368, 535
AvaII GGWCC 2 cut(s) 250, 643
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 2 cut(s) 298, 508
Bbv12I GWGCWC 2 cut(s) 298, 710
BbvI GCAGC 4 cut(s) 419, 639, 937, 996
BccI CCATC 1 cut(s) 2
BceAI ACGGC 2 cut(s) 436, 520
BciT130I CCWGG 2 cut(s) 281, 665
BcnI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
BfmI CTRYAG 2 cut(s) 28, 873
BfoI RGCGCY 1 cut(s) 918
BfuAI ACCTGC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 425
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
Bme18I GGWCC 2 cut(s) 250, 643
BmgBI CACGTC 1 cut(s) 819
BmgT120I GGNCC 4 cut(s) 250, 269, 603, 643
BmiI GGNNCC 9 cut(s) 13, 101, 252, 264, 418, 507, 514, 655, 916
BmrFI CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
BmsI GCATC 2 cut(s) 320, 761
BpmI CTGGAG 2 cut(s) 55, 115
BpuMI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
BsaHI GRCGYC 2 cut(s) 858, 915
BsaJI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BsaWI WCCGGW 4 cut(s) 247, 259, 290, 413
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 285, 315
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
Bse118I RCCGGY 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 2 cut(s) 281, 665
BseDI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BseLI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
BsePI GCGCGC 1 cut(s) 531
BseRI GAGGAG 1 cut(s) 559
BseXI GCAGC 4 cut(s) 419, 639, 937, 996
BseYI CCCAGC 1 cut(s) 953
Bsh1236I CGCG 4 cut(s) 220, 460, 533, 768
Bsh1285I CGRYCG 2 cut(s) 291, 766
BshFI GGCC 4 cut(s) 270, 451, 586, 605
BshNI GGYRCC 1 cut(s) 914
BsiEI CGRYCG 2 cut(s) 291, 766
BsiHKAI GWGCWC 2 cut(s) 298, 710
BslFI GGGAC 2 cut(s) 170, 341
BslI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
BsmFI GGGAC 2 cut(s) 170, 341
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 4 cut(s) 270, 451, 586, 605
Bsp1286I GDGCHC 3 cut(s) 298, 508, 710
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 2 cut(s) 127, 467
BspANI GGCC 4 cut(s) 270, 451, 586, 605
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 4 cut(s) 220, 460, 533, 768
BspHI TCATGA 1 cut(s) 861
BspLI GGNNCC 9 cut(s) 13, 101, 252, 264, 418, 507, 514, 655, 916
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 914
BsrBI CCGCTC 2 cut(s) 391, 399
BsrFI RCCGGY 1 cut(s) 586
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BssHII GCGCGC 1 cut(s) 531
BssMI GATC 2 cut(s) 127, 467
BssNI GRCGYC 2 cut(s) 858, 915
Bst2UI CCWGG 2 cut(s) 281, 665
Bst4CI ACNGT 4 cut(s) 32, 41, 161, 911
Bst6I CTCTTC 3 cut(s) 228, 338, 389
BstACI GRCGYC 2 cut(s) 858, 915
BstC8I GCNNGC 5 cut(s) 119, 421, 533, 588, 925
BstDEI CTNAG 1 cut(s) 996
BstDSI CCRYGG 1 cut(s) 910
BstFNI CGCG 4 cut(s) 220, 460, 533, 768
BstH2I RGCGCY 1 cut(s) 918
BstHHI GCGC 4 cut(s) 533, 535, 917, 984
BstKTI GATC 2 cut(s) 130, 470
BstMBI GATC 2 cut(s) 127, 467
BstMCI CGRYCG 2 cut(s) 291, 766
BstMWI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 768, 956
BstNI CCWGG 2 cut(s) 281, 665
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 9 cut(s) 78, 265, 270, 279, 508, 515, 595, 630, 663
BstSFI CTRYAG 2 cut(s) 28, 873
BstUI CGCG 4 cut(s) 220, 460, 533, 768
BstV1I GCAGC 4 cut(s) 419, 639, 937, 996
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 4 cut(s) 270, 451, 586, 605
BtgI CCRYGG 1 cut(s) 910
BtrI CACGTC 1 cut(s) 819
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 5 cut(s) 119, 421, 533, 588, 925
CciI TCATGA 1 cut(s) 861
CfoI GCGC 4 cut(s) 533, 535, 917, 984
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 4 cut(s) 250, 269, 603, 643
Csp6I GTAC 3 cut(s) 42, 486, 829
CspCI CAANNNNNGTGG 2 cut(s) 892, 927
CviAII CATG 2 cut(s) 331, 862
CviQI GTAC 3 cut(s) 42, 486, 829
DdeI CTNAG 1 cut(s) 996
DinI GGCGCC 1 cut(s) 916
DpnI GATC 2 cut(s) 129, 469
DpnII GATC 2 cut(s) 127, 467
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 2 cut(s) 449, 584
Eam1104I CTCTTC 3 cut(s) 228, 338, 389
EarI CTCTTC 3 cut(s) 228, 338, 389
EciI GGCGGA 6 cut(s) 167, 417, 443, 460, 789, 948
Ecl136II GAGCTC 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 298, 508
Eco47I GGWCC 2 cut(s) 250, 643
Eco53kI GAGCTC 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 279, 663
EcoT38I GRGCYC 2 cut(s) 298, 508
EgeI GGCGCC 1 cut(s) 916
EheI GGCGCC 1 cut(s) 916
FaeI CATG 2 cut(s) 334, 865
FaiI YATR 2 cut(s) 332, 863
FaqI GGGAC 2 cut(s) 170, 341
FatI CATG 2 cut(s) 330, 861
FauI CCCGC 4 cut(s) 126, 196, 210, 225
FblI GTMKAC 2 cut(s) 156, 287
FriOI GRGCYC 2 cut(s) 298, 508
FspI TGCGCA 1 cut(s) 983
GlaI GCGC 4 cut(s) 532, 534, 916, 983
GsaI CCCAGC 1 cut(s) 957
GsuI CTGGAG 2 cut(s) 55, 115
HaeII RGCGCY 1 cut(s) 918
HaeIII GGCC 4 cut(s) 270, 451, 586, 605
HhaI GCGC 4 cut(s) 533, 535, 917, 984
Hin1I GRCGYC 2 cut(s) 858, 915
Hin1II CATG 2 cut(s) 334, 865
Hin6I GCGC 4 cut(s) 531, 533, 915, 982
HinP1I GCGC 4 cut(s) 531, 533, 915, 982
HincII GTYRAC 3 cut(s) 157, 288, 573
HindII GTYRAC 3 cut(s) 157, 288, 573
HinfI GANTC 8 cut(s) 18, 68, 227, 563, 592, 609, 795, 879
HphI GGTGA 2 cut(s) 368, 535
Hpy166II GTNNAC 5 cut(s) 157, 288, 526, 573, 613
Hpy188I TCNGA 4 cut(s) 232, 624, 679, 816
Hpy188III TCNNGA 7 cut(s) 15, 94, 248, 337, 691, 799, 862
Hpy8I GTNNAC 5 cut(s) 157, 288, 526, 573, 613
Hpy99I CGWCG 4 cut(s) 158, 161, 559, 820
HpyAV CCTTC 6 cut(s) 355, 361, 367, 379, 514, 994
HpyCH4III ACNGT 4 cut(s) 32, 41, 161, 911
HpyCH4IV ACGT 2 cut(s) 818, 858
HpyCH4V TGCA 1 cut(s) 785
HpyF10VI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 768, 956
HpyF3I CTNAG 1 cut(s) 996
HpySE526I ACGT 2 cut(s) 818, 858
Hsp92I GRCGYC 2 cut(s) 858, 915
Hsp92II CATG 2 cut(s) 334, 865
HspAI GCGC 4 cut(s) 531, 533, 915, 982
KasI GGCGCC 1 cut(s) 914
Kpn2I TCCGGA 1 cut(s) 247
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Kzo9I GATC 2 cut(s) 127, 467
LguI GCTCTTC 1 cut(s) 389
Lsp1109I GCAGC 4 cut(s) 419, 639, 937, 996
LweI GCATC 2 cut(s) 320, 761
MaeII ACGT 2 cut(s) 818, 858
MaeIII GTNAC 2 cut(s) 854, 944
MalI GATC 2 cut(s) 129, 469
MbiI CCGCTC 2 cut(s) 391, 399
MboI GATC 2 cut(s) 127, 467
MboII GAAGA 6 cut(s) 245, 352, 355, 391, 394, 406
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 3 cut(s) 298, 508, 710
MluCI AATT 2 cut(s) 83, 497
Mly113I GGCGCC 1 cut(s) 915
MlyI GAGTC 6 cut(s) 62, 221, 572, 601, 618, 804
MmeI TCCRAC 2 cut(s) 602, 839
MroI TCCGGA 1 cut(s) 247
MroNI GCCGGC 1 cut(s) 586
MseI TTAA 2 cut(s) 975, 1009
MspA1I CMGCKG 1 cut(s) 923
MspR9I CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 2 cut(s) 281, 665
MvnI CGCG 4 cut(s) 220, 460, 533, 768
MwoI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 768, 956
NaeI GCCGGC 1 cut(s) 588
NarI GGCGCC 1 cut(s) 915
NciI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
NdeII GATC 2 cut(s) 127, 467
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 2 cut(s) 334, 865
NlaIV GGNNCC 9 cut(s) 13, 101, 252, 264, 418, 507, 514, 655, 916
NmeAIII GCCGAG 1 cut(s) 404
NmuCI GTSAC 1 cut(s) 854
NsbI TGCGCA 1 cut(s) 983
NspI RCATGY 1 cut(s) 334
PagI TCATGA 1 cut(s) 861
PasI CCCWGGG 1 cut(s) 664
PauI GCGCGC 1 cut(s) 531
PciI ACATGT 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 389
PctI GAATGC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 18, 879
PflFI GACNNNGTC 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 508
PleI GAGTC 6 cut(s) 62, 221, 571, 600, 617, 803
PluTI GGCGCC 1 cut(s) 918
PpsI GAGTC 6 cut(s) 62, 221, 571, 600, 617, 803
PscI ACATGT 1 cut(s) 330
Psp124BI GAGCTC 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 279, 663
PspFI CCCAGC 1 cut(s) 953
PspGI CCWGG 2 cut(s) 279, 663
PspN4I GGNNCC 9 cut(s) 13, 101, 252, 264, 418, 507, 514, 655, 916
PspPI GGNCC 4 cut(s) 250, 269, 603, 643
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 161
PteI GCGCGC 1 cut(s) 531
RsaI GTAC 3 cut(s) 43, 487, 830
RsaNI GTAC 3 cut(s) 42, 486, 829
SacI GAGCTC 1 cut(s) 298
SalI GTCGAC 2 cut(s) 155, 286
SapI GCTCTTC 1 cut(s) 389
SaqAI TTAA 2 cut(s) 975, 1009
Sau3AI GATC 2 cut(s) 127, 467
Sau96I GGNCC 4 cut(s) 250, 269, 603, 643
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 6 cut(s) 62, 221, 572, 601, 618, 804
ScrFI CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
SduI GDGCHC 3 cut(s) 298, 508, 710
SfaNI GCATC 2 cut(s) 320, 761
SfcI CTRYAG 2 cut(s) 28, 873
SfoI GGCGCC 1 cut(s) 916
SgrDI CGTCGACG 1 cut(s) 155
SinI GGWCC 2 cut(s) 250, 643
Sse9I AATT 2 cut(s) 83, 497
SspDI GGCGCC 1 cut(s) 914
SstI GAGCTC 1 cut(s) 298
StyD4I CCNGG 9 cut(s) 78, 265, 270, 279, 508, 515, 595, 630, 663
TaaI ACNGT 4 cut(s) 32, 41, 161, 911
TaiI ACGT 2 cut(s) 821, 861
TaqI TCGA 6 cut(s) 87, 156, 287, 314, 542, 554
TasI AATT 2 cut(s) 83, 497
TatI WGTACW 1 cut(s) 41
TauI GCSGC 7 cut(s) 402, 762, 771, 774, 888, 920, 923
TfiI GAWTC 2 cut(s) 18, 879
Tru1I TTAA 2 cut(s) 975, 1009
Tru9I TTAA 2 cut(s) 975, 1009
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 4 cut(s) 407, 627, 950, 984
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 2 cut(s) 159, 878
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 250, 643
XapI RAATTY 2 cut(s) 83, 497
XceI RCATGY 1 cut(s) 334
XmiI GTMKAC 2 cut(s) 156, 287
ZraI GACGTC 1 cut(s) 859
ZrmI AGTACT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.