pycom05g31990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006877
Physical Location & Seq
Reverse (-)
31525757 .. 31526827
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31990.1

Sequence Viewer

Length: 1071 bp
ATGGAGCACAGCCCCAAATTCAAACCGCACCTTTCACAGAGCAGCAGCCGAAGAAGGCGAACCAGCACCGATTCCAACTCCCCCGAGTTCGAGTTTTGGAGAAACCCCTCTTGCCCTCAGCCCAATCTCCTCTCCGCCGATGAGCTCTTCGTCGACGGCGTCCTTCTCCCTCTCCACCTCCTCCCCCCTCCTTTCCTTAACCACTCCGACCCTTATCCTAAACATTCCGACCCACCTGACCCAAATCCACAACCCATCTCACAACCACGTGTTCCTGACCCAAAACCCATTTCAGAACCCGACCCAGAATCGGAACCCGGTCCAGGACCTGAGTTGTCAGCTGCTCCCGTTCTAACCGCATCGAAGCGTTGGAGGGACATTTTTAAGATGGGCGAGAAGAAATCCGTCAAAGCCGACGAGACTGATAAAGAGAAAGACAAGAAGAAGGACCGAAAGGGCGGCGGCGGCGGAGCAAGCTCGGCGGAGCTGAACATCAATATATGGCCATTTTCTCGCAGTAGATCCGCCGGAAACGCATATACCCGACCCAAACCGATCGGAGCTCCGGCAACCCGGAAGGTTAACAGCGCTCCGTGTTCAAGGAGCAACTCAACGGGGGAGTCGAAGTCTAGAAAGTGGCCACCTGCGAGTCCGGGTCGACCCGGAGTTCACTTGGGTCGGAGCAGCCCGGTTTGGCAGGTCCGGCGTGGAGCCTCGGCGGCTGTGAAGAGCAACTCCGACCCACAAGCTCGAAATGCTGAAAAGGGCACAAAAAAGGAAGTACCGGAGAATCGCAGGAGTAAAAAAACCGCCGGAGCCGTAGTCGCCGGCGGCAGCGGCTCGAAAGCTAGAGTCTTGAATTTGAATGTTCCGATGTGTATTGGGTATAGAAGCCATTTGAGCTGTAGAAGTGAGAATAGTGCAGTTGGAGTTGGCAATGGCGGCGGTGGAAGCGGCAGCAACAACCGTGGTGGTGATGCTAGGGGCGGCGTTAACGGTGGAGGAGGAAGTGGCGGGGCTGTCGGTAATCTTTTCAATCTGCGCAGCCTTTTCATCAAGAAGGTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

37.86

Weight (kDa)

10.16

Isoelectric Point (pI)

63.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 652
Acc16I TGCGCA 1 cut(s) 1043
Acc36I ACCTGC 2 cut(s) 652, 688
AccI GTMKAC 2 cut(s) 153, 658
AclWI GGATC 1 cut(s) 516
AcoI YGGCCR 2 cut(s) 503, 638
AcsI RAATTY 2 cut(s) 17, 859
AcvI CACGTG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 783
AfeI AGCGCT 1 cut(s) 589
AfiI CCNNNNNNNGG 2 cut(s) 310, 323
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 5 cut(s) 22, 600, 859, 865, 1036
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 274, 306
AluBI AGCT 8 cut(s) 145, 341, 477, 487, 563, 749, 848, 903
AluI AGCT 8 cut(s) 145, 341, 477, 487, 563, 749, 848, 903
Alw21I GWGCWC 3 cut(s) 9, 147, 565
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 1 cut(s) 516
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 589
AoxI GGCC 2 cut(s) 503, 638
ApeKI GCWGC 7 cut(s) 42, 45, 341, 684, 834, 957, 1044
ApoI RAATTY 2 cut(s) 17, 859
AspLEI GCGC 2 cut(s) 590, 1044
AspS9I GGNCC 4 cut(s) 320, 326, 448, 700
AsuC2I CCSGG 5 cut(s) 318, 574, 654, 663, 689
AsuHPI GGTGA 1 cut(s) 986
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 4 cut(s) 320, 326, 448, 700
BaeGI GKGCMC 1 cut(s) 770
BalI TGGCCA 2 cut(s) 505, 640
BanII GRGCYC 2 cut(s) 147, 565
BbrPI CACGTG 1 cut(s) 269
Bbv12I GWGCWC 3 cut(s) 9, 147, 565
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 7 cut(s) 54, 57, 328, 696, 846, 969, 1056
BccI CCATC 2 cut(s) 263, 382
BceAI ACGGC 2 cut(s) 172, 803
BciT130I CCWGG 1 cut(s) 324
BcnI CCSGG 5 cut(s) 318, 574, 654, 663, 689
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 3 cut(s) 630, 849, 981
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 591
BfuAI ACCTGC 2 cut(s) 652, 688
BglI GCCNNNNNGGC 1 cut(s) 719
Bme1390I CCNGG 6 cut(s) 318, 324, 574, 654, 663, 689
Bme18I GGWCC 4 cut(s) 320, 326, 448, 700
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 320, 326, 448, 700
BmiI GGNNCC 3 cut(s) 315, 712, 817
BmrFI CCNGG 6 cut(s) 318, 324, 574, 654, 663, 689
BmsI GCATC 2 cut(s) 368, 967
Bpu10I CCTNAGC 1 cut(s) 117
BpuMI CCSGG 5 cut(s) 318, 574, 654, 663, 689
BsaAI YACGTR 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 714, 967
BsaWI WCCGGW 1 cut(s) 784
BsaXI ACNNNNNCTCC 4 cut(s) 611, 641, 807, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 323
Bse118I RCCGGY 1 cut(s) 827
Bse3DI GCAATG 1 cut(s) 943
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 714, 967
BseLI CCNNNNNNNGG 2 cut(s) 310, 323
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 2 cut(s) 131, 321
BseRI GAGGAG 3 cut(s) 119, 170, 1017
BseSI GKGCMC 1 cut(s) 770
BseXI GCAGC 7 cut(s) 54, 57, 328, 696, 846, 969, 1056
BsgI GTGCAG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 558
BshFI GGCC 2 cut(s) 505, 640
BsiEI CGRYCG 1 cut(s) 558
BsiHKAI GWGCWC 3 cut(s) 9, 147, 565
BsiHKCI CYCGRG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 310, 323
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 2 cut(s) 505, 640
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 4 cut(s) 9, 147, 565, 770
Bsp143I GATC 2 cut(s) 521, 555
BspANI GGCC 2 cut(s) 505, 640
BspCNI CTCAG 2 cut(s) 130, 322
BspLI GGNNCC 3 cut(s) 315, 712, 817
BspMI ACCTGC 2 cut(s) 652, 688
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 2 cut(s) 152, 722
BsrDI GCAATG 1 cut(s) 943
BsrFI RCCGGY 1 cut(s) 827
BssAI RCCGGY 1 cut(s) 827
BssECI CCNNGG 2 cut(s) 714, 967
BssMI GATC 2 cut(s) 521, 555
BssNI GRCGYC 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 968, 998
Bst6I CTCTTC 2 cut(s) 152, 722
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 475, 829
BstDEI CTNAG 2 cut(s) 117, 330
BstDSI CCRYGG 1 cut(s) 967
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 2 cut(s) 590, 1044
BstKTI GATC 2 cut(s) 524, 558
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 2 cut(s) 521, 555
BstMCI CGRYCG 1 cut(s) 558
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 6 cut(s) 316, 322, 572, 652, 661, 687
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 1 cut(s) 770
BstV1I GCAGC 7 cut(s) 54, 57, 328, 696, 846, 969, 1056
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
BsuRI GGCC 2 cut(s) 505, 640
BtgI CCRYGG 1 cut(s) 967
BveI ACCTGC 2 cut(s) 652, 688
Cac8I GCNNGC 2 cut(s) 475, 829
CaiI CAGNNNCTG 1 cut(s) 329
CfoI GCGC 2 cut(s) 590, 1044
Cfr10I RCCGGY 1 cut(s) 827
Cfr13I GGNCC 4 cut(s) 320, 326, 448, 700
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 782
CspCI CAANNNNNGTGG 4 cut(s) 949, 952, 984, 987
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 2 cut(s) 117, 330
DpnI GATC 2 cut(s) 523, 557
DpnII GATC 2 cut(s) 521, 555
EaeI YGGCCR 2 cut(s) 503, 638
Eam1104I CTCTTC 2 cut(s) 152, 722
EarI CTCTTC 2 cut(s) 152, 722
EciI GGCGGA 4 cut(s) 124, 483, 497, 514
Ecl136II GAGCTC 2 cut(s) 145, 563
Eco24I GRGCYC 2 cut(s) 147, 565
Eco47I GGWCC 4 cut(s) 320, 326, 448, 700
Eco47III AGCGCT 1 cut(s) 589
Eco53kI GAGCTC 2 cut(s) 145, 563
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 2 cut(s) 145, 563
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 147, 565
FaiI YATR 5 cut(s) 500, 502, 538, 540, 888
FaqI GGGAC 1 cut(s) 389
FauI CCCGC 1 cut(s) 1007
FblI GTMKAC 2 cut(s) 153, 658
FriOI GRGCYC 2 cut(s) 147, 565
FspBI CTAG 3 cut(s) 630, 849, 981
FspI TGCGCA 1 cut(s) 1043
GlaI GCGC 2 cut(s) 589, 1043
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 2 cut(s) 505, 640
HgaI GACGC 1 cut(s) 148
HhaI GCGC 2 cut(s) 590, 1044
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 2 cut(s) 588, 1042
HinP1I GCGC 2 cut(s) 588, 1042
HincII GTYRAC 4 cut(s) 154, 583, 659, 994
HindII GTYRAC 4 cut(s) 154, 583, 659, 994
HinfI GANTC 6 cut(s) 71, 308, 620, 649, 790, 852
HpaI GTTAAC 2 cut(s) 583, 994
HphI GGTGA 1 cut(s) 986
Hpy166II GTNNAC 5 cut(s) 154, 583, 659, 670, 994
Hpy188I TCNGA 8 cut(s) 208, 229, 295, 313, 560, 681, 739, 873
Hpy188III TCNNGA 4 cut(s) 275, 630, 856, 1057
Hpy8I GTNNAC 5 cut(s) 154, 583, 659, 670, 994
Hpy99I CGWCG 3 cut(s) 155, 158, 419
HpyAV CCTTC 5 cut(s) 48, 173, 439, 571, 1054
HpyCH4III ACNGT 2 cut(s) 968, 998
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 923
HpyF3I CTNAG 2 cut(s) 117, 330
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 2 cut(s) 588, 1042
KroI GCCGGC 1 cut(s) 827
KroNI GCCGGC 1 cut(s) 829
KspAI GTTAAC 2 cut(s) 583, 994
Kzo9I GATC 2 cut(s) 521, 555
LguI GCTCTTC 2 cut(s) 152, 722
Lsp1109I GCAGC 7 cut(s) 54, 57, 328, 696, 846, 969, 1056
LweI GCATC 2 cut(s) 368, 967
MaeI CTAG 3 cut(s) 630, 849, 981
MaeII ACGT 1 cut(s) 268
MalI GATC 2 cut(s) 523, 557
MboI GATC 2 cut(s) 521, 555
MboII GAAGA 5 cut(s) 63, 139, 409, 454, 739
MflI RGATCY 1 cut(s) 521
MhlI GDGCHC 4 cut(s) 9, 147, 565, 770
MlsI TGGCCA 2 cut(s) 505, 640
MluCI AATT 2 cut(s) 17, 859
MluNI TGGCCA 2 cut(s) 505, 640
MlyI GAGTC 3 cut(s) 629, 658, 861
MmeI TCCRAC 7 cut(s) 99, 231, 252, 350, 659, 762, 907
Mox20I TGGCCA 2 cut(s) 505, 640
MreI CGCCGGCG 1 cut(s) 827
MroNI GCCGGC 1 cut(s) 827
MscI TGGCCA 2 cut(s) 505, 640
MseI TTAA 5 cut(s) 198, 384, 582, 993, 1069
Msp20I TGGCCA 2 cut(s) 505, 640
MspA1I CMGCKG 2 cut(s) 341, 837
MspR9I CCNGG 6 cut(s) 318, 324, 574, 654, 663, 689
MvaI CCWGG 1 cut(s) 324
NaeI GCCGGC 1 cut(s) 829
NciI CCSGG 5 cut(s) 318, 574, 654, 663, 689
NdeII GATC 2 cut(s) 521, 555
NgoMIV GCCGGC 1 cut(s) 827
NlaIV GGNNCC 3 cut(s) 315, 712, 817
NmeAIII GCCGAG 2 cut(s) 458, 695
NsbI TGCGCA 1 cut(s) 1043
PaqCI CACCTGC 1 cut(s) 652
PciSI GCTCTTC 2 cut(s) 152, 722
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 620
PdiI GCCGGC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 71, 308, 790
PflFI GACNNNGTC 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 322
Ple19I CGATCG 1 cut(s) 558
PleI GAGTC 3 cut(s) 628, 657, 860
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 3 cut(s) 628, 657, 860
Ppu21I YACGTR 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 147, 565
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 322
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 3 cut(s) 315, 712, 817
PspPI GGNCC 4 cut(s) 320, 326, 448, 700
PspPPI RGGWCCY 1 cut(s) 326
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 521
PsyI GACNNNGTC 1 cut(s) 158
PvuI CGATCG 1 cut(s) 558
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
SacI GAGCTC 2 cut(s) 147, 565
SalI GTCGAC 2 cut(s) 152, 657
SapI GCTCTTC 2 cut(s) 152, 722
SaqAI TTAA 5 cut(s) 198, 384, 582, 993, 1069
Sau3AI GATC 2 cut(s) 521, 555
Sau96I GGNCC 4 cut(s) 320, 326, 448, 700
SchI GAGTC 3 cut(s) 629, 658, 861
ScrFI CCNGG 6 cut(s) 318, 324, 574, 654, 663, 689
SduI GDGCHC 4 cut(s) 9, 147, 565, 770
SfaNI GCATC 2 cut(s) 368, 967
SfcI CTRYAG 1 cut(s) 904
SgrAI CRCCGGYG 1 cut(s) 827
SgrDI CGTCGACG 1 cut(s) 152
SinI GGWCC 4 cut(s) 320, 326, 448, 700
Sse9I AATT 2 cut(s) 17, 859
SspMI CTAG 3 cut(s) 630, 849, 981
SstI GAGCTC 2 cut(s) 147, 565
StyD4I CCNGG 6 cut(s) 316, 322, 572, 652, 661, 687
TaaI ACNGT 2 cut(s) 968, 998
TaiI ACGT 1 cut(s) 271
TaqI TCGA 7 cut(s) 90, 153, 362, 623, 658, 751, 842
TaqII GACCGA 1 cut(s) 465
TasI AATT 2 cut(s) 17, 859
TauI GCSGC 9 cut(s) 462, 465, 468, 722, 834, 840, 945, 957, 990
TfiI GAWTC 3 cut(s) 71, 308, 790
Tru1I TTAA 5 cut(s) 198, 384, 582, 993, 1069
Tru9I TTAA 5 cut(s) 198, 384, 582, 993, 1069
TseI GCWGC 7 cut(s) 42, 45, 341, 684, 834, 957, 1044
TspDTI ATGAA 1 cut(s) 1042
TspGWI ACGGA 2 cut(s) 394, 582
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 4 cut(s) 320, 326, 448, 700
XapI RAATTY 2 cut(s) 17, 859
XbaI TCTAGA 1 cut(s) 629
XmiI GTMKAC 2 cut(s) 153, 658
XspI CTAG 3 cut(s) 630, 849, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.