AT4G25260

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004281
Physical Location & Seq
Reverse (-)
12935710 .. 12937236
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25260.1

Sequence Viewer

Length: 606 bp
ATGGCTAGAAATTTCGAGCTTTCTTTGATTCTCTTTGTTCTGTATCTGTCCACTGCAGCAATAGTCATGGCTAGAAACCTTGAAGAGGAATCAAGTGGTGATACAGAGTTCATTAAAGCCTCTTGTGAGACGACGTCGTACCCAGACCGATGCTTCCAGTCTCTGTCTTCATATGCAAGCGAGATCAAGAAGCAGCCACGTAAGCTTGCTGAGACCGCGCTTGCCGTTAGCATAGCCCGAGCAAAGTCAGCCAAAACCTATGTATCAGAGATGACTGATTATAAAGGAATCACAAAGAGGCAGCACGAGGCTGTAGCGGACTGTCTAGAGGAGATGGGAGACACTGTTGACAGGTTGAGCAATTCGCTGAAGGAACTGAAGCATCTGGAGGAAGGTGACAGCGGAGAAGACTTTTGGTTCTGTCTGAGCAATGTCCGGACGTGGACAAGCGCAGCACTGACAGATGAGACCGCGTGTATGGATGGGTTTGGAGGGAAGGCCATGGCTGGGGAGCTGAAAAGTTTAATCAGAACACACATTGTGAGTGTTGCGGAAGAGACGAGCAATGCCTTGGCTTTGATCAATGACTTTGCTTCCAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0030234 GO:0043086 GO:0044092 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

201

Amino Acids

22.17

Weight (kDa)

5.01

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 193 3.5e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AatII GACGTC 1 cut(s) 137
AccII CGCG 2 cut(s) 218, 473
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 5 cut(s) 216, 317, 402, 471, 551
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 2 cut(s) 389, 398
AcyI GRCGYC 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 2 cut(s) 85, 507
AgsI TTSAA 1 cut(s) 83
AjiI CACGTC 1 cut(s) 441
AluBI AGCT 3 cut(s) 19, 205, 514
AluI AGCT 3 cut(s) 19, 205, 514
Alw26I GTCTC 6 cut(s) 122, 165, 206, 333, 461, 551
AlwNI CAGNNNCTG 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 237
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 4 cut(s) 56, 193, 301, 452
ApoI RAATTY 1 cut(s) 10
AspLEI GCGC 2 cut(s) 220, 452
AsuHPI GGTGA 2 cut(s) 110, 407
AvaI CYCGRG 1 cut(s) 237
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BauI CACGAG 1 cut(s) 305
BbsI GAAGAC 2 cut(s) 159, 414
BbvI GCAGC 4 cut(s) 68, 205, 313, 464
BccI CCATC 2 cut(s) 328, 476
BceAI ACGGC 1 cut(s) 209
BclI TGATCA 1 cut(s) 579
BcoDI GTCTC 6 cut(s) 122, 165, 206, 333, 461, 551
BfaI CTAG 3 cut(s) 6, 72, 326
BfmI CTRYAG 2 cut(s) 54, 312
BisI GCNGC 4 cut(s) 57, 194, 302, 453
BlsI GCNGC 4 cut(s) 58, 195, 303, 454
BmeT110I CYCGRG 1 cut(s) 237
BmgBI CACGTC 1 cut(s) 441
BmsI GCATC 2 cut(s) 140, 391
BpiI GAAGAC 2 cut(s) 159, 414
BpmI CTGGAG 1 cut(s) 407
BsaAI YACGTR 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 134
BsaI GGTCTC 2 cut(s) 206, 461
BsaJI CCNNGG 2 cut(s) 501, 570
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 507
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 2 cut(s) 436, 571
BseAI TCCGGA 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 501, 570
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 2 cut(s) 85, 507
BseMI GCAATG 2 cut(s) 436, 571
BseMII CTCAG 2 cut(s) 201, 416
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 4 cut(s) 68, 205, 313, 464
BseYI CCCAGC 1 cut(s) 506
Bsh1236I CGCG 2 cut(s) 218, 473
BshFI GGCC 1 cut(s) 500
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 85, 507
BsmAI GTCTC 6 cut(s) 122, 165, 206, 333, 461, 551
BsmBI CGTCTC 2 cut(s) 122, 551
BsnI GGCC 1 cut(s) 500
Bso31I GGTCTC 2 cut(s) 206, 461
BsoBI CYCGRG 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 2 cut(s) 183, 579
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 5 cut(s) 216, 317, 402, 471, 551
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 2 cut(s) 202, 417
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 2 cut(s) 218, 473
BspMAI CTGCAG 1 cut(s) 58
BspTNI GGTCTC 2 cut(s) 206, 461
BsrDI GCAATG 2 cut(s) 436, 571
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 501, 570
BssMI GATC 2 cut(s) 183, 579
BssNI GRCGYC 1 cut(s) 134
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 2 cut(s) 501, 570
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 323, 346
Bst6I CTCTTC 2 cut(s) 78, 549
BstACI GRCGYC 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 200
BstC8I GCNNGC 3 cut(s) 178, 207, 222
BstDEI CTNAG 2 cut(s) 210, 425
BstDSI CCRYGG 1 cut(s) 501
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 487
BstFNI CGCG 2 cut(s) 218, 473
BstHHI GCGC 2 cut(s) 220, 452
BstKTI GATC 2 cut(s) 186, 582
BstMAI GTCTC 6 cut(s) 122, 165, 206, 333, 461, 551
BstMBI GATC 2 cut(s) 183, 579
BstMWI GCNNNNNNNGC 3 cut(s) 202, 215, 248
BstSFI CTRYAG 2 cut(s) 54, 312
BstUI CGCG 2 cut(s) 218, 473
BstV1I GCAGC 4 cut(s) 68, 205, 313, 464
BstV2I GAAGAC 2 cut(s) 159, 414
BsuRI GGCC 1 cut(s) 500
BtgI CCRYGG 1 cut(s) 501
BtrI CACGTC 1 cut(s) 441
BtsCI GGATG 1 cut(s) 487
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 3 cut(s) 51, 342, 455
Cac8I GCNNGC 3 cut(s) 178, 207, 222
CaiI CAGNNNCTG 1 cut(s) 163
CfoI GCGC 2 cut(s) 220, 452
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 67, 502
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 210, 425
DpnI GATC 2 cut(s) 185, 581
DpnII GATC 2 cut(s) 183, 579
DraIII CACNNNGTG 1 cut(s) 541
Eam1104I CTCTTC 2 cut(s) 78, 549
EarI CTCTTC 2 cut(s) 78, 549
Eco130I CCWWGG 2 cut(s) 501, 570
Eco31I GGTCTC 2 cut(s) 206, 461
Eco57I CTGAAG 2 cut(s) 389, 398
Eco88I CYCGRG 1 cut(s) 237
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoT14I CCWWGG 2 cut(s) 501, 570
ErhI CCWWGG 2 cut(s) 501, 570
Esp3I CGTCTC 2 cut(s) 122, 551
FaeI CATG 2 cut(s) 70, 505
FaiI YATR 8 cut(s) 68, 172, 174, 233, 261, 282, 479, 503
FatI CATG 2 cut(s) 66, 501
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 4 cut(s) 57, 194, 302, 453
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 4 cut(s) 57, 194, 302, 453
FspBI CTAG 3 cut(s) 6, 72, 326
GlaI GCGC 2 cut(s) 219, 451
GluI GCNGC 4 cut(s) 57, 194, 302, 453
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 1 cut(s) 500
HapII CCGG 1 cut(s) 436
HhaI GCGC 2 cut(s) 220, 452
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 2 cut(s) 70, 505
Hin6I GCGC 2 cut(s) 218, 450
HinP1I GCGC 2 cut(s) 218, 450
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 3 cut(s) 28, 89, 288
HpaII CCGG 1 cut(s) 436
HphI GGTGA 2 cut(s) 110, 407
Hpy166II GTNNAC 3 cut(s) 51, 349, 444
Hpy188I TCNGA 3 cut(s) 268, 426, 530
Hpy188III TCNNGA 4 cut(s) 187, 326, 386, 436
Hpy8I GTNNAC 3 cut(s) 51, 349, 444
Hpy99I CGWCG 2 cut(s) 136, 139
HpyAV CCTTC 3 cut(s) 364, 386, 490
HpyCH4III ACNGT 2 cut(s) 323, 346
HpyCH4IV ACGT 3 cut(s) 134, 199, 440
HpyCH4V TGCA 2 cut(s) 56, 176
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 215, 248
HpyF3I CTNAG 2 cut(s) 210, 425
HpySE526I ACGT 3 cut(s) 134, 199, 440
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 2 cut(s) 70, 505
HspAI GCGC 2 cut(s) 218, 450
Kpn2I TCCGGA 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 2 cut(s) 183, 579
LmnI GCTCC 1 cut(s) 511
LpnPI CCDG 6 cut(s) 156, 170, 337, 371, 449, 492
Lsp1109I GCAGC 4 cut(s) 68, 205, 313, 464
LweI GCATC 2 cut(s) 140, 391
MaeI CTAG 3 cut(s) 6, 72, 326
MaeII ACGT 3 cut(s) 134, 199, 440
MaeIII GTNAC 1 cut(s) 395
MalI GATC 2 cut(s) 185, 581
MboI GATC 2 cut(s) 183, 579
MboII GAAGA 4 cut(s) 95, 159, 419, 566
MluCI AATT 2 cut(s) 10, 361
MnlI CCTC 7 cut(s) 79, 130, 291, 301, 322, 382, 485
MroI TCCGGA 1 cut(s) 435
MseI TTAA 2 cut(s) 114, 524
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 218, 473
MwoI GCNNNNNNNGC 3 cut(s) 202, 215, 248
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 172
NdeII GATC 2 cut(s) 183, 579
NlaIII CATG 2 cut(s) 70, 505
NmuCI GTSAC 1 cut(s) 395
PfeI GAWTC 3 cut(s) 28, 89, 288
PflFI GACNNNGTC 1 cut(s) 133
PkrI GCNGC 4 cut(s) 58, 195, 303, 454
Ppu21I YACGTR 1 cut(s) 200
PsiI TTATAA 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 506
PstI CTGCAG 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 163
PsyI GACNNNGTC 1 cut(s) 133
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 114, 524
SatI GCNGC 4 cut(s) 57, 194, 302, 453
Sau3AI GATC 2 cut(s) 183, 579
SfaNI GCATC 2 cut(s) 140, 391
SfcI CTRYAG 2 cut(s) 54, 312
Sse9I AATT 2 cut(s) 10, 361
SsiI CCGC 5 cut(s) 216, 317, 402, 471, 551
SspMI CTAG 3 cut(s) 6, 72, 326
StyI CCWWGG 2 cut(s) 501, 570
TaaI ACNGT 2 cut(s) 323, 346
TaiI ACGT 3 cut(s) 137, 202, 443
TaqI TCGA 1 cut(s) 15
TaqII GACCGA 1 cut(s) 162
TasI AATT 2 cut(s) 10, 361
TfiI GAWTC 3 cut(s) 28, 89, 288
Tru1I TTAA 2 cut(s) 114, 524
Tru9I TTAA 2 cut(s) 114, 524
TscAI CASTG 3 cut(s) 58, 349, 462
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 4 cut(s) 56, 193, 301, 452
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 100, 159
TspRI CASTG 3 cut(s) 58, 349, 462
Tth111I GACNNNGTC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 325
XspI CTAG 3 cut(s) 6, 72, 326
ZraI GACGTC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.