Rh1DG391000

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004281
Physical Location & Seq
Reverse (-)
60946668 .. 60948242
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG391000.1

Sequence Viewer

Length: 741 bp
ATGATATGCCATGTATATCCAGATGAAATTTTCATTTATTTTATTTTTGTGTGTGGTCGGTTTGTTCCTATAAATTGGTACCTTTGGCTCCCTAATTTCATATCACTCCCCTCCCAAAATTTGAAAAGAGAATCAAAGAAAGAAATGGCCTCCTCATCTTTCACATCTCTTTTTATAGTACTTTCCATTAGTTGCTGCATATTCTACTCAACCTCAGCAGCTAGAGACCTTCCACCAAAAACCAACACTGAGTTCATGAAAACATCTTGTAGTGCAACAAGATACCCAAAACTTTGTCTTACTTCCCTTGCAAGCCAAGCAACTGCAATCCAAACAAGTCCGAAACTCATGGCGGCTGCAGCCCTTAGCGTGACACTTTCCTCTGCTAAATCAACCTCTACCATGATGTTGAGTCTCTCCCACAGCCACGGAATGAAGCCTAGAGAAGTTGCTGCCATGAGGGACTGCGTTGAAGTGTTGAGCGATTCTGTGGACGAGCTTCAGAGGTCTATTTCCGAGATGGCTAACTTTAAGAGCTCGGATTTTGAGATGATGATAAGTGATGTACAAACTTGGGTTAGCGCGGCCTTAACGGATGAGAGTACCTGCACGGATGGGTTTGCTGGAAATGGGTTGAACGGGAATTTGAAGACTGTTGTGAGGGGGAAGATTGTGAACATTGCACAATTGACTAGCAATGCCTTGGCCTTAATAAACAGATACGCCTCAGTTCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

246

Amino Acids

26.98

Weight (kDa)

8.1

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 85 - 237 7.5e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 614
Acc65I GGTACC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 78
AccII CGCG 1 cut(s) 584
AciI CCGC 2 cut(s) 353, 584
AcsI RAATTY 3 cut(s) 27, 118, 643
AcuI CTGAAG 1 cut(s) 485
AfaI GTAC 4 cut(s) 80, 180, 567, 604
AgsI TTSAA 4 cut(s) 124, 473, 637, 649
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 3 cut(s) 221, 499, 537
AluI AGCT 3 cut(s) 221, 499, 537
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 219, 419
AoxI GGCC 3 cut(s) 147, 585, 705
ApeKI GCWGC 5 cut(s) 195, 218, 356, 359, 452
ApoI RAATTY 3 cut(s) 27, 118, 643
Asp718I GGTACC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 539
BbvCI CCTCAGC 1 cut(s) 214
BbvI GCAGC 5 cut(s) 182, 230, 343, 371, 439
BccI CCATC 2 cut(s) 514, 608
BcoDI GTCTC 2 cut(s) 219, 419
BfaI CTAG 3 cut(s) 222, 441, 693
BfmI CTRYAG 1 cut(s) 357
BfuAI ACCTGC 1 cut(s) 614
BisI GCNGC 7 cut(s) 196, 219, 354, 357, 360, 453, 585
BlsI GCNGC 7 cut(s) 197, 220, 355, 358, 361, 454, 586
BmcAI AGTACT 1 cut(s) 180
BmiI GGNNCC 2 cut(s) 80, 89
BpiI GAAGAC 1 cut(s) 656
Bpu10I CCTNAGC 2 cut(s) 214, 365
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 427, 702
Bse3DI GCAATG 2 cut(s) 678, 703
BseDI CCNNGG 2 cut(s) 427, 702
BseGI GGATG 2 cut(s) 601, 619
BseMI GCAATG 2 cut(s) 678, 703
BseMII CTCAG 3 cut(s) 228, 240, 741
BseRI GAGGAG 1 cut(s) 142
BseXI GCAGC 5 cut(s) 182, 230, 343, 371, 439
BsgI GTGCAG 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 584
BshFI GGCC 3 cut(s) 149, 587, 707
BshNI GGYRCC 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 539
BslFI GGGAC 1 cut(s) 476
BsmAI GTCTC 2 cut(s) 219, 419
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 3 cut(s) 149, 587, 707
Bso31I GGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 565
BspACI CCGC 2 cut(s) 353, 584
BspANI GGCC 3 cut(s) 149, 587, 707
BspCNI CTCAG 3 cut(s) 227, 241, 740
BspFNI CGCG 1 cut(s) 584
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 80, 89
BspMAI CTGCAG 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 614
BspT107I GGYRCC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 219
BsrDI GCAATG 2 cut(s) 678, 703
BsrGI TGTACA 1 cut(s) 565
BssECI CCNNGG 2 cut(s) 427, 702
BssT1I CCWWGG 1 cut(s) 702
Bst4CI ACNGT 1 cut(s) 655
BstAUI TGTACA 1 cut(s) 565
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 214, 249, 365, 727
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 2 cut(s) 601, 619
BstFNI CGCG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 584
BstMAI GTCTC 2 cut(s) 219, 419
BstMWI GCNNNNNNNGC 2 cut(s) 317, 359
BstSFI CTRYAG 1 cut(s) 357
BstUI CGCG 1 cut(s) 584
BstV1I GCAGC 5 cut(s) 182, 230, 343, 371, 439
BstV2I GAAGAC 1 cut(s) 656
BsuRI GGCC 3 cut(s) 149, 587, 707
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 2 cut(s) 601, 619
BtsIMutI CAGTG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 1 cut(s) 313
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 584
Csp6I GTAC 4 cut(s) 79, 179, 566, 603
CviAII CATG 6 cut(s) 11, 256, 349, 403, 457, 734
CviQI GTAC 4 cut(s) 79, 179, 566, 603
DdeI CTNAG 4 cut(s) 214, 249, 365, 727
Ecl136II GAGCTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 702
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 537
EcoT14I CCWWGG 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 6 cut(s) 14, 259, 352, 406, 460, 737
FaqI GGGAC 1 cut(s) 476
FatI CATG 6 cut(s) 10, 255, 348, 402, 456, 733
Fnu4HI GCNGC 7 cut(s) 196, 219, 354, 357, 360, 453, 585
FokI GGATG 2 cut(s) 608, 626
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 7 cut(s) 196, 219, 354, 357, 360, 453, 585
FspBI CTAG 3 cut(s) 222, 441, 693
GlaI GCGC 1 cut(s) 583
GluI GCNGC 7 cut(s) 196, 219, 354, 357, 360, 453, 585
HaeIII GGCC 3 cut(s) 149, 587, 707
HhaI GCGC 1 cut(s) 584
Hin1II CATG 6 cut(s) 14, 259, 352, 406, 460, 737
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HinfI GANTC 3 cut(s) 131, 412, 485
Hpy166II GTNNAC 2 cut(s) 493, 676
Hpy188I TCNGA 4 cut(s) 342, 504, 517, 541
Hpy188III TCNNGA 2 cut(s) 20, 256
Hpy8I GTNNAC 2 cut(s) 493, 676
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4V TGCA 7 cut(s) 198, 275, 311, 326, 359, 609, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 359
HpyF3I CTNAG 4 cut(s) 214, 249, 365, 727
Hsp92II CATG 6 cut(s) 14, 259, 352, 406, 460, 737
HspAI GCGC 1 cut(s) 582
KpnI GGTACC 1 cut(s) 82
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 3 cut(s) 33, 609, 619
Lsp1109I GCAGC 5 cut(s) 182, 230, 343, 371, 439
MaeI CTAG 3 cut(s) 222, 441, 693
MaeIII GTNAC 1 cut(s) 370
MboII GAAGA 2 cut(s) 661, 679
MfeI CAATTG 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 6 cut(s) 27, 73, 94, 118, 643, 686
MlyI GAGTC 1 cut(s) 421
MseI TTAA 4 cut(s) 531, 590, 710, 739
MunI CAATTG 1 cut(s) 686
MvnI CGCG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 317, 359
NlaIII CATG 6 cut(s) 14, 259, 352, 406, 460, 737
NlaIV GGNNCC 2 cut(s) 80, 89
NmuCI GTSAC 1 cut(s) 370
PagI TCATGA 1 cut(s) 255
PfeI GAWTC 2 cut(s) 131, 485
PkrI GCNGC 7 cut(s) 197, 220, 355, 358, 361, 454, 586
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 539
PspN4I GGNNCC 2 cut(s) 80, 89
PstI CTGCAG 1 cut(s) 361
RsaI GTAC 4 cut(s) 80, 180, 567, 604
RsaNI GTAC 4 cut(s) 79, 179, 566, 603
SacI GAGCTC 1 cut(s) 539
SaqAI TTAA 4 cut(s) 531, 590, 710, 739
SatI GCNGC 7 cut(s) 196, 219, 354, 357, 360, 453, 585
ScaI AGTACT 1 cut(s) 180
SchI GAGTC 1 cut(s) 421
SduI GDGCHC 1 cut(s) 539
SetI ASST 9 cut(s) 84, 215, 223, 231, 398, 501, 509, 539, 608
SfcI CTRYAG 1 cut(s) 357
Sse9I AATT 6 cut(s) 27, 73, 94, 118, 643, 686
SsiI CCGC 2 cut(s) 353, 584
SspMI CTAG 3 cut(s) 222, 441, 693
SstI GAGCTC 1 cut(s) 539
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 1 cut(s) 655
TasI AATT 6 cut(s) 27, 73, 94, 118, 643, 686
TatI WGTACW 2 cut(s) 178, 565
TauI GCSGC 2 cut(s) 356, 587
TfiI GAWTC 2 cut(s) 131, 485
Tru1I TTAA 4 cut(s) 531, 590, 710, 739
Tru9I TTAA 4 cut(s) 531, 590, 710, 739
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 5 cut(s) 195, 218, 356, 359, 452
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 7 cut(s) 22, 39, 88, 244, 272, 449, 722
TspGWI ACGGA 3 cut(s) 444, 608, 626
TspRI CASTG 1 cut(s) 253
XapI RAATTY 3 cut(s) 27, 118, 643
XspI CTAG 3 cut(s) 222, 441, 693
ZrmI AGTACT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.