Rmu_sc0003443.1_g000004

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003443.1
OG0004281
Physical Location & Seq
Reverse (-)
5959 .. 6297
339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003443.1_g000004.1.cds

Sequence Viewer

Length: 339 bp
atgatgttaaggctctcccacggccatggcatgaagcctagagaagttgctgccatgagggactgcgtcgaactgttgagtgactcggtggacgagcttcagaggtctatttcgaagatggttaactttaggagttctgggtttgagcttatgataagtgatgttcaaacttgggttagtgctgccttgacggatgagggcacctgctcggatgggtttgctggaaatggattgaatgggaatttgaagactgttgtgaggggtcggattgtgaacattgcacaattgactagcaatgccttggctttgattaacagatacgcctcggttcatagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

112

Amino Acids

12.17

Weight (kDa)

6.82

Isoelectric Point (pI)

17.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 167, 235, 247
AluBI AGCT 2 cut(s) 97, 148
AluI AGCT 2 cut(s) 97, 148
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 2 cut(s) 50, 182
ApoI RAATTY 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 37, 169
BccI CCATC 2 cut(s) 112, 206
BceAI ACGGC 1 cut(s) 37
BfaI CTAG 2 cut(s) 39, 291
BfuAI ACCTGC 1 cut(s) 212
BisI GCNGC 2 cut(s) 51, 183
BlsI GCNGC 2 cut(s) 52, 184
BmiI GGNNCC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 19, 25, 300, 324
Bse3DI GCAATG 2 cut(s) 276, 301
BseDI CCNNGG 4 cut(s) 19, 25, 300, 324
BseGI GGATG 2 cut(s) 199, 217
BseMI GCAATG 2 cut(s) 276, 301
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 2 cut(s) 37, 169
BshFI GGCC 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 200
BslFI GGGAC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 203
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 276, 301
BssECI CCNNGG 4 cut(s) 19, 25, 300, 324
BssT1I CCWWGG 2 cut(s) 25, 300
Bst4CI ACNGT 2 cut(s) 75, 253
BstBI TTCGAA 1 cut(s) 113
BstDSI CCRYGG 2 cut(s) 19, 25
BstF5I GGATG 2 cut(s) 199, 217
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 37, 169
BstV2I GAAGAC 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 26
BsuRI GGCC 1 cut(s) 24
BtgI CCRYGG 2 cut(s) 19, 25
BtsCI GGATG 2 cut(s) 199, 217
BveI ACCTGC 1 cut(s) 212
CseI GACGC 1 cut(s) 55
CviAII CATG 3 cut(s) 26, 31, 55
CviJI RGCY 6 cut(s) 13, 24, 37, 97, 148, 305
CviKI_1 RGCY 6 cut(s) 13, 24, 37, 97, 148, 305
EaeI YGGCCR 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 25, 300
Eco57I CTGAAG 1 cut(s) 83
EcoT14I CCWWGG 2 cut(s) 25, 300
ErhI CCWWGG 2 cut(s) 25, 300
FaeI CATG 3 cut(s) 29, 34, 58
FaiI YATR 5 cut(s) 27, 32, 56, 152, 333
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 25, 30, 54
Fnu4HI GCNGC 2 cut(s) 51, 183
FokI GGATG 2 cut(s) 206, 224
Fsp4HI GCNGC 2 cut(s) 51, 183
FspBI CTAG 2 cut(s) 39, 291
GluI GCNGC 2 cut(s) 51, 183
HaeIII GGCC 1 cut(s) 24
HgaI GACGC 1 cut(s) 55
Hin1II CATG 3 cut(s) 29, 34, 58
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 83
HpaI GTTAAC 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 91, 124, 274
Hpy188I TCNGA 3 cut(s) 102, 211, 267
Hpy8I GTNNAC 3 cut(s) 91, 124, 274
Hpy99I CGWCG 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 75, 253
HpyCH4V TGCA 1 cut(s) 281
Hsp92II CATG 3 cut(s) 29, 34, 58
KspAI GTTAAC 1 cut(s) 124
LpnPI CCDG 3 cut(s) 123, 207, 217
Lsp1109I GCAGC 2 cut(s) 37, 169
MaeI CTAG 2 cut(s) 39, 291
MaeIII GTNAC 1 cut(s) 80
MboII GAAGA 2 cut(s) 127, 259
MfeI CAATTG 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 2 cut(s) 241, 284
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 5 cut(s) 51, 96, 190, 252, 334
MseI TTAA 3 cut(s) 8, 123, 312
MslI CAYNNNNRTG 1 cut(s) 24
MunI CAATTG 1 cut(s) 284
NcoI CCATGG 1 cut(s) 25
NlaIII CATG 3 cut(s) 29, 34, 58
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 113
PaqCI CACCTGC 1 cut(s) 212
PflFI GACNNNGTC 1 cut(s) 65
PkrI GCNGC 2 cut(s) 52, 184
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 202
PsyI GACNNNGTC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 24
SaqAI TTAA 3 cut(s) 8, 123, 312
SatI GCNGC 2 cut(s) 51, 183
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 1 cut(s) 203
SetI ASST 4 cut(s) 99, 107, 150, 206
SfuI TTCGAA 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 2 cut(s) 241, 284
SspMI CTAG 2 cut(s) 39, 291
StyI CCWWGG 2 cut(s) 25, 300
TaaI ACNGT 2 cut(s) 75, 253
TaqI TCGA 2 cut(s) 69, 113
TasI AATT 2 cut(s) 241, 284
Tru1I TTAA 3 cut(s) 8, 123, 312
Tru9I TTAA 3 cut(s) 8, 123, 312
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 50, 182
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 47, 320
TspGWI ACGGA 1 cut(s) 206
Tth111I GACNNNGTC 1 cut(s) 65
XapI RAATTY 1 cut(s) 241
XspI CTAG 2 cut(s) 39, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.