MD00G1105200.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004281
Physical Location & Seq
Forward (+)
22001473 .. 22001781
309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1105200.v1.1.491

Sequence Viewer

Length: 309 bp
ATGAAGCCTAGAGAAGTCGGAGCCATGAGGGACTGCATCGAAGAGTTAAGTGATTCGGTCGATGCAATTCAGAGGTCCATAGCTGAGATGGGCAACTTTAGGAGCTCGGATTTTCAGTTCATGATAAGTGATGTGCAAACTTGGGTTAGTGCTGCTTTGACGGATGAGACAACATGCTTTGATGGGTTTGGTGGAAATGGGATGAACGGTAATTTGAAGACTGTTGTGAGGGGCAGAGTTGTGAATATTGCACAATTGACTAGCAATGCCTTGGCTTTGATCAACAGATACGCCTCAGTTTATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

103

Amino Acids

11.13

Weight (kDa)

4.73

Isoelectric Point (pI)

18.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 5 - 93 1.2e-23 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 1 cut(s) 217
AloI GAACNNNNNNTCC 2 cut(s) 101, 133
AluBI AGCT 2 cut(s) 83, 105
AluI AGCT 2 cut(s) 83, 105
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 75
AvaII GGWCC 1 cut(s) 75
BanII GRGCYC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 82, 176
BclI TGATCA 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 9, 261
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 2 cut(s) 45, 52
BpiI GAAGAC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 169, 207
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 75, 309
BseXI GCAGC 1 cut(s) 139
Bsh1285I CGRYCG 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 107
BslFI GGGAC 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 279
BspCNI CTCAG 2 cut(s) 76, 308
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 270
Bst4CI ACNGT 2 cut(s) 209, 223
Bst6I CTCTTC 1 cut(s) 36
BstDEI CTNAG 2 cut(s) 84, 295
BstF5I GGATG 2 cut(s) 169, 207
BstKTI GATC 1 cut(s) 282
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 279
BstMCI CGRYCG 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 224
BtsCI GGATG 2 cut(s) 169, 207
CciI TCATGA 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 75
CviAII CATG 3 cut(s) 25, 121, 174
CviJI RGCY 5 cut(s) 7, 23, 83, 105, 275
CviKI_1 RGCY 5 cut(s) 7, 23, 83, 105, 275
DdeI CTNAG 2 cut(s) 84, 295
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 3 cut(s) 28, 124, 177
FaiI YATR 5 cut(s) 26, 80, 122, 175, 303
FaqI GGGAC 1 cut(s) 44
FatI CATG 3 cut(s) 24, 120, 173
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 2 cut(s) 176, 214
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 2 cut(s) 9, 261
GluI GCNGC 1 cut(s) 153
Hin1II CATG 3 cut(s) 28, 124, 177
HinfI GANTC 1 cut(s) 53
Hpy188I TCNGA 3 cut(s) 20, 72, 109
Hpy188III TCNNGA 1 cut(s) 121
HpyCH4III ACNGT 2 cut(s) 209, 223
HpyCH4V TGCA 4 cut(s) 36, 65, 136, 251
HpyF3I CTNAG 2 cut(s) 84, 295
Hsp92II CATG 3 cut(s) 28, 124, 177
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 1 cut(s) 279
LmnI GCTCC 2 cut(s) 20, 102
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 2 cut(s) 45, 52
MaeI CTAG 2 cut(s) 9, 261
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 2 cut(s) 53, 229
MfeI CAATTG 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 66, 211, 254
MnlI CCTC 4 cut(s) 21, 66, 222, 304
MseI TTAA 2 cut(s) 47, 307
MunI CAATTG 1 cut(s) 254
NdeII GATC 1 cut(s) 279
NlaIII CATG 3 cut(s) 28, 124, 177
NlaIV GGNNCC 1 cut(s) 22
NspI RCATGY 1 cut(s) 177
PagI TCATGA 1 cut(s) 120
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 75
SacI GAGCTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 47, 307
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 279
Sau96I GGNCC 1 cut(s) 75
SduI GDGCHC 1 cut(s) 107
SetI ASST 3 cut(s) 77, 85, 107
SfaNI GCATC 2 cut(s) 45, 52
SgeI CNNG 8 cut(s) 21, 37, 118, 133, 153, 186, 273, 283
SinI GGWCC 1 cut(s) 75
Sse9I AATT 3 cut(s) 66, 211, 254
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 9, 261
SstI GAGCTC 1 cut(s) 107
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 209, 223
TaqI TCGA 2 cut(s) 39, 60
TaqII GACCGA 1 cut(s) 46
TasI AATT 3 cut(s) 66, 211, 254
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 2 cut(s) 47, 307
Tru9I TTAA 2 cut(s) 47, 307
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 3 cut(s) 17, 109, 218
TspGWI ACGGA 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 75
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 2 cut(s) 9, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.