AT5G62350

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004281
Physical Location & Seq
Forward (+)
25037191 .. 25038424
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62350.1

Sequence Viewer

Length: 609 bp
ATGGCAAAACAATATCTCTTTGTACTCCTCTCAATCTCCTATCTCTTATCACTGGAGCTCACGGCGGCCACCGCAGCCTCACAGACCGGAGCTTCCAAAAAAGCCATAAACTTCATCCAATCTTCTTGCAAAACCACCACATACCCTGCCTTATGTGTCCACTCACTCTCCGTCTACGCAAACGACATCCAAACAAGCCCTAAACGTTTAGCTGAGACCGCTATAGCCGTGACACTAAGCCGAGCCCAATCCACGAAGCTCTTCGTCTCGCGTCTAACACGTATGAAGGGTCTTAAGAAGCGCGAGGTCGAAGCCATCAAAGATTGCGTCGAGGAGATGAACGATACCGTTGACCGTTTGACCAAATCTGTTCAAGAACTGAAGTTGTGTGGGAGTGCCAAAGATCAAGACCAGTTTGCGTACCACATGAGTAATGCTCAGACTTGGACTAGTGCGGCTTTGACTGACGAGAACACTTGCTCCGATGGGTTCTCGGGTCGGGTTATGGATGGGAGGATCAAGAACTCGGTTCGGGCTAGAATCATGAACGTGGGACATGAAACCAGCAACGCTTTGTCCTTGATTAATGCCTTTGCTAAAACTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

202

Amino Acids

22.18

Weight (kDa)

9.21

Isoelectric Point (pI)

24.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 194 1.1e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 271, 303
AciI CCGC 4 cut(s) 65, 72, 219, 455
AclI AACGTT 1 cut(s) 205
AclWI GGATC 1 cut(s) 524
AcoI YGGCCR 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 24, 422
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 1 cut(s) 374
AhlI ACTAGT 1 cut(s) 449
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 58, 92, 212, 259
AluI AGCT 4 cut(s) 58, 92, 212, 259
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 209, 271
AlwI GGATC 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 493
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 1 cut(s) 303
AvaI CYCGRG 1 cut(s) 493
BanII GRGCYC 2 cut(s) 60, 247
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 86
BccI CCATC 3 cut(s) 323, 479, 503
BceAI ACGGC 2 cut(s) 78, 212
BcoDI GTCTC 2 cut(s) 209, 271
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 2 cut(s) 450, 537
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 3 cut(s) 66, 75, 456
BlsI GCNGC 3 cut(s) 67, 76, 457
BmeT110I CYCGRG 1 cut(s) 493
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 1 cut(s) 74
BsaAI YACGTR 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 464, 494
Bse1I ACTGG 2 cut(s) 57, 412
BseGI GGATG 3 cut(s) 114, 186, 514
BseMII CTCAG 2 cut(s) 204, 452
BseNI ACTGG 2 cut(s) 57, 412
BseRI GAGGAG 2 cut(s) 17, 347
BseXI GCAGC 1 cut(s) 86
Bsh1236I CGCG 2 cut(s) 271, 303
BshFI GGCC 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 493
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 567
BsmAI GTCTC 2 cut(s) 209, 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 567
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 60, 247
Bsp143I GATC 2 cut(s) 403, 516
BspACI CCGC 4 cut(s) 65, 72, 219, 455
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 205, 451
BspFNI CGCG 2 cut(s) 271, 303
BspHI TCATGA 1 cut(s) 543
BspPI GGATC 1 cut(s) 524
BspQI GCTCTTC 1 cut(s) 266
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 209
BsrI ACTGG 2 cut(s) 57, 412
BssMI GATC 2 cut(s) 403, 516
Bst4CI ACNGT 2 cut(s) 349, 356
Bst6I CTCTTC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 281
BstDEI CTNAG 3 cut(s) 213, 236, 438
BstF5I GGATG 3 cut(s) 114, 186, 514
BstFNI CGCG 2 cut(s) 271, 303
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 2 cut(s) 406, 519
BstMAI GTCTC 2 cut(s) 209, 271
BstMBI GATC 2 cut(s) 403, 516
BstMWI GCNNNNNNNGC 3 cut(s) 71, 74, 218
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 2 cut(s) 271, 303
BstV1I GCAGC 1 cut(s) 86
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 3 cut(s) 114, 186, 514
BtsIMutI CAGTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 543
CfoI GCGC 1 cut(s) 303
CseI GACGC 2 cut(s) 260, 316
Csp6I GTAC 2 cut(s) 23, 421
CviAII CATG 3 cut(s) 427, 544, 557
CviQI GTAC 2 cut(s) 23, 421
DdeI CTNAG 3 cut(s) 213, 236, 438
DpnI GATC 2 cut(s) 405, 518
DpnII GATC 2 cut(s) 403, 516
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 60, 247
Eco31I GGTCTC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 58
EcoT38I GRGCYC 2 cut(s) 60, 247
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 3 cut(s) 430, 547, 560
FaiI YATR 9 cut(s) 107, 142, 154, 224, 284, 428, 506, 545, 558
FaqI GGGAC 1 cut(s) 567
FatI CATG 3 cut(s) 426, 543, 556
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 66, 75, 456
FokI GGATG 3 cut(s) 101, 173, 521
FriOI GRGCYC 2 cut(s) 60, 247
Fsp4HI GCNGC 3 cut(s) 66, 75, 456
FspBI CTAG 2 cut(s) 450, 537
GlaI GCGC 1 cut(s) 302
GluI GCNGC 3 cut(s) 66, 75, 456
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 87
HgaI GACGC 2 cut(s) 260, 316
HhaI GCGC 1 cut(s) 303
Hin1II CATG 3 cut(s) 430, 547, 560
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 540
HpaII CCGG 1 cut(s) 87
Hpy166II GTNNAC 3 cut(s) 160, 175, 352
Hpy188I TCNGA 2 cut(s) 441, 484
Hpy188III TCNNGA 4 cut(s) 374, 407, 520, 544
Hpy8I GTNNAC 3 cut(s) 160, 175, 352
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 2 cut(s) 349, 356
HpyCH4IV ACGT 3 cut(s) 205, 280, 549
HpyCH4V TGCA 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 74, 218
HpyF3I CTNAG 3 cut(s) 213, 236, 438
HpySE526I ACGT 3 cut(s) 205, 280, 549
Hsp92II CATG 3 cut(s) 430, 547, 560
HspAI GCGC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 403, 516
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 3 cut(s) 55, 89, 485
LpnPI CCDG 5 cut(s) 38, 100, 159, 425, 577
Lsp1109I GCAGC 1 cut(s) 86
MaeI CTAG 2 cut(s) 450, 537
MaeII ACGT 3 cut(s) 205, 280, 549
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 405, 518
MboI GATC 2 cut(s) 403, 516
MboII GAAGA 2 cut(s) 114, 253
MhlI GDGCHC 2 cut(s) 60, 247
MnlI CCTC 5 cut(s) 38, 88, 298, 325, 507
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 2 cut(s) 294, 585
MslI CAYNNNNRTG 1 cut(s) 548
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 1 cut(s) 87
MvnI CGCG 2 cut(s) 271, 303
MwoI GCNNNNNNNGC 3 cut(s) 71, 74, 218
NdeII GATC 2 cut(s) 403, 516
NlaIII CATG 3 cut(s) 430, 547, 560
NmeAIII GCCGAG 1 cut(s) 266
NmuCI GTSAC 1 cut(s) 229
PagI TCATGA 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 540
PkrI GCNGC 3 cut(s) 67, 76, 457
Ppu21I YACGTR 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 60
Psp1406I AACGTT 1 cut(s) 205
RsaI GTAC 2 cut(s) 24, 422
RsaNI GTAC 2 cut(s) 23, 421
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 2 cut(s) 294, 585
SatI GCNGC 3 cut(s) 66, 75, 456
Sau3AI GATC 2 cut(s) 403, 516
SduI GDGCHC 2 cut(s) 60, 247
SetI ASST 8 cut(s) 60, 94, 208, 214, 261, 283, 309, 552
SfcI CTRYAG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SpeI ACTAGT 1 cut(s) 449
SsiI CCGC 4 cut(s) 65, 72, 219, 455
SspMI CTAG 2 cut(s) 450, 537
SstI GAGCTC 1 cut(s) 60
TaaI ACNGT 2 cut(s) 349, 356
TaiI ACGT 3 cut(s) 208, 283, 552
TaqI TCGA 2 cut(s) 309, 330
TatI WGTACW 1 cut(s) 22
TauI GCSGC 2 cut(s) 68, 458
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 2 cut(s) 294, 585
Tru9I TTAA 2 cut(s) 294, 585
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 5 cut(s) 103, 299, 353, 560, 573
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 57
Vha464I CTTAAG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 585
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 2 cut(s) 450, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.