AT5G62340

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004281
Physical Location & Seq
Forward (+)
25033471 .. 25034404
934 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62340.1

Sequence Viewer

Length: 621 bp
ATGGCAAAACAATACCAAGCTCTCTTTCTACTCTTCTCCGTCTTCTACCTCTTCTCGTCGGTGCTCACGACCGCCACAGTCAACCCGGCCGGAACTACCACAAAAGCACTAAACTTTATTCAATCCTCATGCAAATCCACAACATACCAATCCCTGTGCGTTGAAACTCTCTCGGTTTACGCCAATACGATCAAAACAAGCCCTCGACACCTCTTGGATGCAGCTATCACCGTGAGTCTGAACCAAGCGCTGTCCACGAAGCTCTTCATCTCACACCTGAGGAAGAGCCAGTTCCAAATCCTTCAGGACTGCGCACCATCGACCGATACGTTCAGTACAGATTGCGAGTGTTCTGTCCAAGCACTCCAAGAGGTGGTGAATTGTAACAGTTGGACCGACTGCTTGTTTCACGTAAAGAATGCTGAAGTGTGTGCCATTTCGGGTGAGAGTCATAGCGTCGAGAACACTTGCTCCAGCCCGTTCGCGGATCCGGGAAAGATATCAGCCAGGGGCCGGATCTCGGATGCCGTAAGAAAATCACTTCATACCCGGTTCTCTAAGTTGCGCCAGGAAATAAACAATGCCAAAATGTTGTTCGAGGCCTTTCCTAATAAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

206

Amino Acids

22.76

Weight (kDa)

8.58

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 195 3.9e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 373
AccII CGCG 1 cut(s) 485
AciI CCGC 2 cut(s) 72, 485
AclWI GGATC 3 cut(s) 482, 495, 524
AcoI YGGCCR 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 287, 444
AfaI GTAC 1 cut(s) 337
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 373, 484, 513, 520
AgsI TTSAA 2 cut(s) 122, 164
AjnI CCWGG 2 cut(s) 506, 567
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 578, 610
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 3 cut(s) 20, 224, 262
AluI AGCT 3 cut(s) 20, 224, 262
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 3 cut(s) 482, 495, 524
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 3 cut(s) 87, 511, 600
ApeKI GCWGC 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 250, 314, 567
AspS9I GGNCC 2 cut(s) 393, 511
AsuC2I CCSGG 3 cut(s) 86, 492, 550
AsuHPI GGTGA 3 cut(s) 220, 388, 455
AvaII GGWCC 1 cut(s) 393
AxyI CCTNAGG 1 cut(s) 278
BamHI GGATCC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 325
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 2 cut(s) 508, 569
BcnI CCSGG 3 cut(s) 86, 492, 550
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 5 cut(s) 86, 492, 508, 550, 569
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 393, 511
BmiI GGNNCC 2 cut(s) 489, 512
BmrFI CCNGG 5 cut(s) 86, 492, 508, 550, 569
BmsI GCATC 2 cut(s) 208, 514
BpiI GAAGAC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 457
BpuMI CCSGG 3 cut(s) 86, 492, 550
BsaAI YACGTR 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 484, 513, 520
Bse1I ACTGG 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 278
BseBI CCWGG 2 cut(s) 508, 569
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 223, 529
BseLI CCNNNNNNNGG 4 cut(s) 373, 484, 513, 520
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 289
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 3 cut(s) 72, 90, 324
BshFI GGCC 3 cut(s) 89, 513, 602
BsiEI CGRYCG 3 cut(s) 72, 90, 324
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 5 cut(s) 86, 90, 491, 514, 550
BslI CCNNNNNNNGG 4 cut(s) 373, 484, 513, 520
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 3 cut(s) 89, 513, 602
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 3 cut(s) 189, 487, 516
BspACI CCGC 2 cut(s) 72, 485
BspANI GGCC 3 cut(s) 89, 513, 602
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 2 cut(s) 489, 512
BspPI GGATC 3 cut(s) 482, 495, 524
BspQI GCTCTTC 2 cut(s) 269, 278
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 3 cut(s) 189, 487, 516
Bst2UI CCWGG 2 cut(s) 508, 569
Bst4CI ACNGT 3 cut(s) 79, 232, 389
Bst6I CTCTTC 4 cut(s) 38, 56, 269, 278
BstBAI YACGTR 1 cut(s) 412
BstDEI CTNAG 2 cut(s) 278, 558
BstF5I GGATG 2 cut(s) 223, 529
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 3 cut(s) 250, 314, 567
BstKTI GATC 3 cut(s) 192, 490, 519
BstMBI GATC 3 cut(s) 189, 487, 516
BstMCI CGRYCG 3 cut(s) 72, 90, 324
BstNI CCWGG 2 cut(s) 508, 569
BstSCI CCNGG 5 cut(s) 84, 490, 506, 548, 567
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 2 cut(s) 487, 516
BstYI RGATCY 2 cut(s) 487, 516
BstZI CGGCCG 1 cut(s) 87
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 3 cut(s) 89, 513, 602
BtsCI GGATG 2 cut(s) 223, 529
CfoI GCGC 3 cut(s) 250, 314, 567
Cfr13I GGNCC 2 cut(s) 393, 511
CseI GACGC 1 cut(s) 445
Csp6I GTAC 1 cut(s) 336
CviAII CATG 1 cut(s) 129
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 2 cut(s) 278, 558
DpnI GATC 3 cut(s) 191, 489, 518
DpnII GATC 3 cut(s) 189, 487, 516
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 4 cut(s) 38, 56, 269, 278
EarI CTCTTC 4 cut(s) 38, 56, 269, 278
EclXI CGGCCG 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 602
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 393
Eco47III AGCGCT 1 cut(s) 249
Eco52I CGGCCG 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 287, 444
Eco81I CCTNAGG 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 506, 567
EcoRV GATATC 1 cut(s) 501
FaeI CATG 1 cut(s) 132
FaiI YATR 4 cut(s) 130, 145, 453, 546
FatI CATG 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 230, 536
Fsp4HI GCNGC 1 cut(s) 222
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 249, 313, 566
GluI GCNGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 3 cut(s) 89, 513, 602
HapII CCGG 5 cut(s) 86, 90, 491, 514, 550
HgaI GACGC 1 cut(s) 445
HhaI GCGC 3 cut(s) 250, 314, 567
Hin1II CATG 1 cut(s) 132
Hin6I GCGC 3 cut(s) 248, 312, 565
HinP1I GCGC 3 cut(s) 248, 312, 565
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 2 cut(s) 235, 448
HpaII CCGG 5 cut(s) 86, 90, 491, 514, 550
HphI GGTGA 3 cut(s) 220, 388, 455
Hpy166II GTNNAC 3 cut(s) 82, 178, 255
Hpy188I TCNGA 2 cut(s) 240, 523
Hpy188III TCNNGA 3 cut(s) 67, 305, 460
Hpy8I GTNNAC 3 cut(s) 82, 178, 255
Hpy99I CGWCG 2 cut(s) 61, 461
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 3 cut(s) 79, 232, 389
HpyCH4IV ACGT 2 cut(s) 329, 411
HpyCH4V TGCA 2 cut(s) 132, 221
HpyF3I CTNAG 2 cut(s) 278, 558
HpySE526I ACGT 2 cut(s) 329, 411
Hsp92II CATG 1 cut(s) 132
HspAI GCGC 3 cut(s) 248, 312, 565
Kzo9I GATC 3 cut(s) 189, 487, 516
LguI GCTCTTC 2 cut(s) 269, 278
LmnI GCTCC 1 cut(s) 476
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 208, 514
MaeII ACGT 2 cut(s) 329, 411
MaeIII GTNAC 1 cut(s) 383
MalI GATC 3 cut(s) 191, 489, 518
MboI GATC 3 cut(s) 189, 487, 516
MboII GAAGA 5 cut(s) 25, 34, 43, 256, 295
MflI RGATCY 2 cut(s) 487, 516
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 1 cut(s) 379
MlyI GAGTC 2 cut(s) 244, 457
MmeI TCCRAC 1 cut(s) 371
MnlI CCTC 7 cut(s) 59, 136, 213, 221, 273, 364, 592
MroXI GAANNNNTTC 1 cut(s) 263
MspI CCGG 5 cut(s) 86, 90, 491, 514, 550
MspR9I CCNGG 5 cut(s) 86, 492, 508, 550, 569
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 2 cut(s) 508, 569
MvnI CGCG 1 cut(s) 485
NciI CCSGG 3 cut(s) 86, 492, 550
NdeII GATC 3 cut(s) 189, 487, 516
NlaIII CATG 1 cut(s) 132
NlaIV GGNNCC 2 cut(s) 489, 512
NsbI TGCGCA 1 cut(s) 313
PceI AGGCCT 1 cut(s) 602
PciSI GCTCTTC 2 cut(s) 269, 278
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 326
PctI GAATGC 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 263
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 2 cut(s) 243, 456
PpsI GAGTC 2 cut(s) 243, 456
Ppu21I YACGTR 1 cut(s) 412
Psp6I CCWGG 2 cut(s) 506, 567
PspGI CCWGG 2 cut(s) 506, 567
PspN4I GGNNCC 2 cut(s) 489, 512
PspPI GGNCC 2 cut(s) 393, 511
PsuI RGATCY 2 cut(s) 487, 516
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SapI GCTCTTC 2 cut(s) 269, 278
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 3 cut(s) 189, 487, 516
Sau96I GGNCC 2 cut(s) 393, 511
SchI GAGTC 2 cut(s) 244, 457
ScrFI CCNGG 5 cut(s) 86, 492, 508, 550, 569
SduI GDGCHC 1 cut(s) 66
SetI ASST 9 cut(s) 22, 51, 213, 226, 264, 279, 332, 375, 414
SfaNI GCATC 2 cut(s) 208, 514
SinI GGWCC 1 cut(s) 393
Sse9I AATT 1 cut(s) 379
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 2 cut(s) 72, 485
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 5 cut(s) 84, 490, 506, 548, 567
TaaI ACNGT 3 cut(s) 79, 232, 389
TaiI ACGT 2 cut(s) 332, 414
TaqI TCGA 4 cut(s) 205, 320, 459, 597
TaqII GACCGA 2 cut(s) 338, 410
TasI AATT 1 cut(s) 379
TatI WGTACW 1 cut(s) 335
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 256, 533
TspGWI ACGGA 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.