FvH4_7g27800

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004281
Physical Location & Seq
Reverse (-)
20719705 .. 20720316
612 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27800.t1

Sequence Viewer

Length: 612 bp
ATGGAACCCTCATTCTCTAAACATTTCTTCACTTCTTCTCTCATAGTGTTACTCTTCACTACGTGCAGCATATTCTCCTCAGTCTCAGCTGCTAGAGACCTTGCTCCCAAAACCAATACCGAATTCATCGAAACATCATGTGGTGCAACAACCTATCCAAAACTATGCCTTGCTTCCTTGTCAAGCCAAGCAAGTGTCATCCAAAGAAGTCCTAAACTTATAGCTTGTGCTGCACTCAATGTCACGCTTTCGTCTGCTAAATCGACATCTGCGATGATGTTGAGGCTCTCACACGGTTATGGCATGAAGCCTAGAGAAGCTGCAGCCATGAGGGACTGCGTCGAAATGGTGAGTGACTCGGTGGACGAGATTCAGAGGTCTATTTCCGAGATGGTTAACTTTAGAAGTGCTGGTTTTGAGATGATGATAAGTGATGTACAAACTTGGGTTAGTGCTGCCTTAACAGATGAGAGCACCTGCTCGGATGGGTTTGCTGGAAATGAATTGAATGGGAATTTGAAGACTGTTGTGAGGGGTCGGATTGTGAAGATTGCACAATTGACTAGCAATGCCTTGGCTTTAATCAACAGATACGCCTCGGTTCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

204

Amino Acids

21.83

Weight (kDa)

7.61

Isoelectric Point (pI)

26.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 42 - 194 1.5e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 485
Acc36I ACCTGC 1 cut(s) 485
AcsI RAATTY 2 cut(s) 122, 514
AdeI CACNNNGTG 1 cut(s) 63
AfaI GTAC 1 cut(s) 438
AgsI TTSAA 2 cut(s) 508, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 89, 224, 320
AluI AGCT 3 cut(s) 89, 224, 320
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 2 cut(s) 88, 90
ApeKI GCWGC 6 cut(s) 66, 89, 230, 320, 323, 455
ApoI RAATTY 2 cut(s) 122, 514
AsuHPI GGTGA 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 476
BbvI GCAGC 6 cut(s) 76, 78, 217, 307, 335, 442
BccI CCATC 2 cut(s) 385, 479
BcoDI GTCTC 2 cut(s) 88, 90
BfaI CTAG 3 cut(s) 93, 312, 564
BfmI CTRYAG 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 485
BisI GCNGC 6 cut(s) 67, 90, 231, 321, 324, 456
BlsI GCNGC 6 cut(s) 68, 91, 232, 322, 325, 457
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 527
BsaAI YACGTR 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 573, 597
Bse3DI GCAATG 1 cut(s) 574
BseDI CCNNGG 2 cut(s) 573, 597
BseGI GGATG 2 cut(s) 198, 490
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 93, 99
BseRI GAGGAG 1 cut(s) 67
BseXI GCAGC 6 cut(s) 76, 78, 217, 307, 335, 442
BsgI GTGCAG 2 cut(s) 85, 216
BsiHKAI GWGCWC 1 cut(s) 476
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 2 cut(s) 88, 90
BsmFI GGGAC 1 cut(s) 347
Bso31I GGTCTC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 476
Bsp1407I TGTACA 1 cut(s) 436
BspCNI CTCAG 2 cut(s) 92, 98
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 325
BspMI ACCTGC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 574
BsrGI TGTACA 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 573, 597
BssT1I CCWWGG 1 cut(s) 573
Bst4CI ACNGT 2 cut(s) 296, 526
Bst6I CTCTTC 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 436
BstBAI YACGTR 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 79, 85
BstF5I GGATG 2 cut(s) 198, 490
BstMAI GTCTC 2 cut(s) 88, 90
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 6 cut(s) 76, 78, 217, 307, 335, 442
BstV2I GAAGAC 1 cut(s) 527
BtgZI GCGATG 1 cut(s) 287
BtsCI GGATG 2 cut(s) 198, 490
BveI ACCTGC 1 cut(s) 485
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 437
CviAII CATG 4 cut(s) 138, 304, 328, 605
CviJI RGCY 8 cut(s) 89, 186, 224, 286, 310, 320, 326, 578
CviKI_1 RGCY 8 cut(s) 89, 186, 224, 286, 310, 320, 326, 578
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 2 cut(s) 79, 85
DraIII CACNNNGTG 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 573
Eco31I GGTCTC 1 cut(s) 90
EcoRI GAATTC 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 4 cut(s) 141, 307, 331, 608
FaiI YATR 9 cut(s) 44, 71, 139, 166, 221, 300, 305, 329, 606
FaqI GGGAC 1 cut(s) 347
FatI CATG 4 cut(s) 137, 303, 327, 604
Fnu4HI GCNGC 6 cut(s) 67, 90, 231, 321, 324, 456
FokI GGATG 2 cut(s) 185, 497
Fsp4HI GCNGC 6 cut(s) 67, 90, 231, 321, 324, 456
FspBI CTAG 3 cut(s) 93, 312, 564
GluI GCNGC 6 cut(s) 67, 90, 231, 321, 324, 456
HgaI GACGC 1 cut(s) 328
Hin1II CATG 4 cut(s) 141, 307, 331, 608
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 356, 370
HpaI GTTAAC 1 cut(s) 397
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 364, 397
Hpy188I TCNGA 4 cut(s) 375, 388, 484, 540
Hpy8I GTNNAC 2 cut(s) 364, 397
Hpy99I CGWCG 1 cut(s) 344
HpyCH4III ACNGT 2 cut(s) 296, 526
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 66, 146, 233, 323, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 2 cut(s) 79, 85
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 4 cut(s) 141, 307, 331, 608
KspAI GTTAAC 1 cut(s) 397
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 3 cut(s) 396, 480, 490
Lsp1109I GCAGC 6 cut(s) 76, 78, 217, 307, 335, 442
MaeI CTAG 3 cut(s) 93, 312, 564
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 3 cut(s) 48, 241, 353
MboII GAAGA 5 cut(s) 19, 27, 46, 532, 559
MfeI CAATTG 1 cut(s) 557
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 4 cut(s) 122, 503, 514, 557
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 7 cut(s) 19, 88, 276, 324, 369, 525, 607
MseI TTAA 3 cut(s) 396, 461, 581
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 89
MunI CAATTG 1 cut(s) 557
MwoI GCNNNNNNNGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 141, 307, 331, 608
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 241, 353
PaqCI CACCTGC 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PfeI GAWTC 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 338
PkrI GCNGC 6 cut(s) 68, 91, 232, 322, 325, 457
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Ppu21I YACGTR 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 325
PsyI GACNNNGTC 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 3 cut(s) 396, 461, 581
SatI GCNGC 6 cut(s) 67, 90, 231, 321, 324, 456
SchI GAGTC 1 cut(s) 350
SduI GDGCHC 1 cut(s) 476
SetI ASST 8 cut(s) 65, 91, 102, 155, 226, 322, 380, 479
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 297
Sse9I AATT 4 cut(s) 122, 503, 514, 557
SspMI CTAG 3 cut(s) 93, 312, 564
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 2 cut(s) 296, 526
TaiI ACGT 1 cut(s) 65
TaqI TCGA 3 cut(s) 129, 263, 342
TasI AATT 4 cut(s) 122, 503, 514, 557
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 3 cut(s) 396, 461, 581
Tru9I TTAA 3 cut(s) 396, 461, 581
TseFI GTSAC 2 cut(s) 241, 353
TseI GCWGC 6 cut(s) 66, 89, 230, 320, 323, 455
Tsp45I GTSAC 2 cut(s) 241, 353
TspDTI ATGAA 4 cut(s) 115, 320, 516, 593
Tth111I GACNNNGTC 1 cut(s) 338
XapI RAATTY 2 cut(s) 122, 514
XspI CTAG 3 cut(s) 93, 312, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.