Rw1G035100

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004281
Physical Location & Seq
Reverse (-)
62941280 .. 62942643
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035100.1

Sequence Viewer

Length: 597 bp
ATGGCCTCCTCATCTTTCACATCTCTTCTTATAGTACTTTCCATTAGTTGCTGCATATTCTACTCAACCTCAGCAGCTAGAGACCTTCCACCAAAAACCAACACTGAGTTCATGAAAACATCTTGTAGTGCAACAAGATACCCAAAACTTTGTCTTACTTCCCTTGCAAGCCAAGCAACTGCAATCCAAACAAGTCCGAAACTCATGGCGGCTGCAGCCCTTAGCGTGACACTTTCCTCTGCTAAATCAACCTCTACCATGATGTTGAGTCTCTCCCACAGCCACGGAATGAAGCCTAGAGAAGTTGCTGCCATGAGGGACTGCGTTGAAGTGTTGAGCGATTCTGTGGACGAGCTTCAGAGGTCTATTTCCGAGATGGCTAACTTTAAGAGCTCGGATTTTGAGATGATGATAAGTGATGTACAAACTTGGGTTAGCGCGGCCTTAACGGATGAGAGTACCTGCACAGATGGGTTTGCTGGAAATGGGTTGAACGGGAATTTGAAGACTGTTGTGAGGGGGAAGATTGTAAACATTGCACAATTGACTAGCAATGCCTTGGCCTTAATCAACAGATACGCCTCAGTTCACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.07

Weight (kDa)

7.6

Isoelectric Point (pI)

25.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 189 3.8e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 470
AccII CGCG 1 cut(s) 440
AciI CCGC 2 cut(s) 209, 440
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 3 cut(s) 36, 423, 460
AgsI TTSAA 3 cut(s) 329, 493, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 3 cut(s) 77, 355, 393
AluI AGCT 3 cut(s) 77, 355, 393
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 2 cut(s) 75, 275
AoxI GGCC 3 cut(s) 3, 441, 561
ApeKI GCWGC 5 cut(s) 51, 74, 212, 215, 308
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 395
BbvCI CCTCAGC 1 cut(s) 70
BbvI GCAGC 5 cut(s) 38, 86, 199, 227, 295
BccI CCATC 2 cut(s) 370, 464
BcoDI GTCTC 2 cut(s) 75, 275
BfaI CTAG 3 cut(s) 78, 297, 549
BfmI CTRYAG 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 470
BisI GCNGC 7 cut(s) 52, 75, 210, 213, 216, 309, 441
BlsI GCNGC 7 cut(s) 53, 76, 211, 214, 217, 310, 442
BmcAI AGTACT 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 512
Bpu10I CCTNAGC 2 cut(s) 70, 221
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 283, 558
Bse3DI GCAATG 2 cut(s) 534, 559
BseDI CCNNGG 2 cut(s) 283, 558
BseGI GGATG 1 cut(s) 457
BseMI GCAATG 2 cut(s) 534, 559
BseMII CTCAG 3 cut(s) 84, 96, 597
BseXI GCAGC 5 cut(s) 38, 86, 199, 227, 295
BsgI GTGCAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 3 cut(s) 5, 443, 563
BsiHKAI GWGCWC 1 cut(s) 395
BslFI GGGAC 1 cut(s) 332
BsmAI GTCTC 2 cut(s) 75, 275
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 5, 443, 563
Bso31I GGTCTC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 421
BspACI CCGC 2 cut(s) 209, 440
BspANI GGCC 3 cut(s) 5, 443, 563
BspCNI CTCAG 3 cut(s) 83, 97, 596
BspFNI CGCG 1 cut(s) 440
BspHI TCATGA 1 cut(s) 111
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 2 cut(s) 534, 559
BsrGI TGTACA 1 cut(s) 421
BssECI CCNNGG 2 cut(s) 283, 558
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 2 cut(s) 511, 593
Bst6I CTCTTC 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 421
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 4 cut(s) 70, 105, 221, 583
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 1 cut(s) 440
BstMAI GTCTC 2 cut(s) 75, 275
BstMWI GCNNNNNNNGC 2 cut(s) 173, 215
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 5 cut(s) 38, 86, 199, 227, 295
BstV2I GAAGAC 1 cut(s) 512
BsuRI GGCC 3 cut(s) 5, 443, 563
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 457
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 111
CfoI GCGC 1 cut(s) 440
Csp6I GTAC 3 cut(s) 35, 422, 459
CviAII CATG 4 cut(s) 112, 205, 259, 313
CviQI GTAC 3 cut(s) 35, 422, 459
DdeI CTNAG 4 cut(s) 70, 105, 221, 583
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 558
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 4 cut(s) 115, 208, 262, 316
FaiI YATR 6 cut(s) 32, 56, 113, 206, 260, 314
FaqI GGGAC 1 cut(s) 332
FatI CATG 4 cut(s) 111, 204, 258, 312
Fnu4HI GCNGC 7 cut(s) 52, 75, 210, 213, 216, 309, 441
FokI GGATG 1 cut(s) 464
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 7 cut(s) 52, 75, 210, 213, 216, 309, 441
FspBI CTAG 3 cut(s) 78, 297, 549
GlaI GCGC 1 cut(s) 439
GluI GCNGC 7 cut(s) 52, 75, 210, 213, 216, 309, 441
HaeIII GGCC 3 cut(s) 5, 443, 563
HhaI GCGC 1 cut(s) 440
Hin1II CATG 4 cut(s) 115, 208, 262, 316
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 2 cut(s) 268, 341
Hpy166II GTNNAC 3 cut(s) 349, 532, 589
Hpy188I TCNGA 4 cut(s) 198, 360, 373, 397
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 3 cut(s) 349, 532, 589
HpyAV CCTTC 1 cut(s) 95
HpyCH4III ACNGT 2 cut(s) 511, 593
HpyCH4V TGCA 7 cut(s) 54, 131, 167, 182, 215, 465, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 215
HpyF3I CTNAG 4 cut(s) 70, 105, 221, 583
Hsp92II CATG 4 cut(s) 115, 208, 262, 316
HspAI GCGC 1 cut(s) 438
LpnPI CCDG 2 cut(s) 465, 475
Lsp1109I GCAGC 5 cut(s) 38, 86, 199, 227, 295
MaeI CTAG 3 cut(s) 78, 297, 549
MaeIII GTNAC 1 cut(s) 226
MboII GAAGA 3 cut(s) 17, 517, 535
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 2 cut(s) 499, 542
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 9 cut(s) 16, 19, 79, 247, 262, 309, 354, 510, 592
MseI TTAA 4 cut(s) 387, 446, 566, 595
MunI CAATTG 1 cut(s) 542
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 173, 215
NlaIII CATG 4 cut(s) 115, 208, 262, 316
NmuCI GTSAC 1 cut(s) 226
PagI TCATGA 1 cut(s) 111
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 7 cut(s) 53, 76, 211, 214, 217, 310, 442
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 217
RsaI GTAC 3 cut(s) 36, 423, 460
RsaNI GTAC 3 cut(s) 35, 422, 459
SacI GAGCTC 1 cut(s) 395
SaqAI TTAA 4 cut(s) 387, 446, 566, 595
SatI GCNGC 7 cut(s) 52, 75, 210, 213, 216, 309, 441
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 277
SduI GDGCHC 1 cut(s) 395
SetI ASST 8 cut(s) 71, 79, 87, 254, 357, 365, 395, 464
SfcI CTRYAG 1 cut(s) 213
Sse9I AATT 2 cut(s) 499, 542
SsiI CCGC 2 cut(s) 209, 440
SspMI CTAG 3 cut(s) 78, 297, 549
SstI GAGCTC 1 cut(s) 395
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 511, 593
TasI AATT 2 cut(s) 499, 542
TatI WGTACW 2 cut(s) 34, 421
TauI GCSGC 2 cut(s) 212, 443
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 4 cut(s) 387, 446, 566, 595
Tru9I TTAA 4 cut(s) 387, 446, 566, 595
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 5 cut(s) 51, 74, 212, 215, 308
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 100, 128, 305
TspGWI ACGGA 2 cut(s) 300, 464
TspRI CASTG 1 cut(s) 109
XapI RAATTY 1 cut(s) 499
XspI CTAG 3 cut(s) 78, 297, 549
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.