Rh1AG395600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004281
Physical Location & Seq
Reverse (-)
62371170 .. 62372126
957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG395600.1

Sequence Viewer

Length: 612 bp
ATGGAACCCTCATTTTCTAAACATTTCTTTACATCTTCTCTCATAGTACTACTCTTCACTACTTGTAGCATATTCTGCTCAATCTCAGCAGCTAGAGACCTTGCCCCGAAAACCAATACCGAGTTCATTAAAACATCTTGTGGTGCAACAACCTATCCAAAACTCTGCCTTGCCTCTCTCTCAAGCCAAGCAAGTGTCATCCAAACAAGTCCTAAACTAATTGCTTGTGCAGCCCTTAATGTGACACTTTCGTCTGCTAAATCAACCTCCACTATGATGTTAAGGCTCTCCCACGGCCATGGCATGAAGCCTAGAGAAGTTGCTGCCATGAGGGACTGCGTCGAACTGTTGAGTGAGTCGGTGGACGAGCTTCAGAGGTCTATTTCGAAGATGGTTAACTTTAGGAGTTCTGGGTTTGAGCTTATGATAAGTGATGTTCAAACTTGGGTTAGTGCTGCCTTGACGGATGAGGGCACCTGCTCGGATGGGTTTGCTGGAAATGGATTGAATGGGAATTTGAAGACTGTTGTGAGGGGTCGGATTGTGAACATTGCACAATTGACTAGCAATGCCTTGGCTTTGATTAACAGATACGCCTCGGTTCATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.75

Weight (kDa)

8.73

Isoelectric Point (pI)

25.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 42 - 194 2.8e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 485
AasI GACNNNNNNGTC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 473
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 1 cut(s) 514
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 1 cut(s) 48
AgsI TTSAA 3 cut(s) 440, 508, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 92, 370, 421
AluI AGCT 3 cut(s) 92, 370, 421
Alw26I GTCTC 1 cut(s) 90
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 4 cut(s) 89, 230, 323, 455
ApoI RAATTY 1 cut(s) 514
AsuII TTCGAA 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 4 cut(s) 101, 242, 310, 442
BccI CCATC 2 cut(s) 385, 479
BceAI ACGGC 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 3 cut(s) 93, 312, 564
BfuAI ACCTGC 1 cut(s) 485
BisI GCNGC 4 cut(s) 90, 231, 324, 456
BlsI GCNGC 4 cut(s) 91, 232, 325, 457
BmcAI AGTACT 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 6, 475
BpiI GAAGAC 1 cut(s) 527
Bpu14I TTCGAA 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 4 cut(s) 292, 298, 573, 597
Bse3DI GCAATG 2 cut(s) 549, 574
BseDI CCNNGG 4 cut(s) 292, 298, 573, 597
BseGI GGATG 3 cut(s) 198, 472, 490
BseMI GCAATG 2 cut(s) 549, 574
BseMII CTCAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 4 cut(s) 101, 242, 310, 442
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 1 cut(s) 297
BshNI GGYRCC 1 cut(s) 473
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 1 cut(s) 297
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 476
Bsp19I CCATGG 1 cut(s) 298
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 98
BspLI GGNNCC 2 cut(s) 6, 475
BspMI ACCTGC 1 cut(s) 485
BspT104I TTCGAA 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 2 cut(s) 549, 574
BssECI CCNNGG 4 cut(s) 292, 298, 573, 597
BssT1I CCWWGG 2 cut(s) 298, 573
Bst4CI ACNGT 2 cut(s) 348, 526
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 386
BstDEI CTNAG 1 cut(s) 85
BstDSI CCRYGG 2 cut(s) 292, 298
BstF5I GGATG 3 cut(s) 198, 472, 490
BstMAI GTCTC 1 cut(s) 90
BstMWI GCNNNNNNNGC 2 cut(s) 75, 230
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 4 cut(s) 101, 242, 310, 442
BstV2I GAAGAC 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 299
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 2 cut(s) 292, 298
BtsCI GGATG 3 cut(s) 198, 472, 490
BveI ACCTGC 1 cut(s) 485
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 47
CviAII CATG 3 cut(s) 299, 304, 328
CviJI RGCY 9 cut(s) 92, 186, 233, 286, 297, 310, 370, 421, 578
CviKI_1 RGCY 9 cut(s) 92, 186, 233, 286, 297, 310, 370, 421, 578
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 1 cut(s) 85
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 298, 573
Eco31I GGTCTC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 356
EcoT14I CCWWGG 2 cut(s) 298, 573
ErhI CCWWGG 2 cut(s) 298, 573
FaeI CATG 3 cut(s) 302, 307, 331
FaiI YATR 8 cut(s) 44, 71, 275, 300, 305, 329, 425, 606
FaqI GGGAC 1 cut(s) 347
FatI CATG 3 cut(s) 298, 303, 327
Fnu4HI GCNGC 4 cut(s) 90, 231, 324, 456
FokI GGATG 3 cut(s) 185, 479, 497
Fsp4HI GCNGC 4 cut(s) 90, 231, 324, 456
FspBI CTAG 3 cut(s) 93, 312, 564
GluI GCNGC 4 cut(s) 90, 231, 324, 456
HaeIII GGCC 1 cut(s) 297
HgaI GACGC 1 cut(s) 328
Hin1II CATG 3 cut(s) 302, 307, 331
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 1 cut(s) 356
HpaI GTTAAC 1 cut(s) 397
Hpy166II GTNNAC 3 cut(s) 364, 397, 547
Hpy188I TCNGA 3 cut(s) 375, 484, 540
Hpy8I GTNNAC 3 cut(s) 364, 397, 547
Hpy99I CGWCG 1 cut(s) 344
HpyCH4III ACNGT 2 cut(s) 348, 526
HpyCH4V TGCA 3 cut(s) 146, 230, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 230
HpyF3I CTNAG 1 cut(s) 85
Hsp92II CATG 3 cut(s) 302, 307, 331
KspAI GTTAAC 1 cut(s) 397
LpnPI CCDG 3 cut(s) 396, 480, 490
Lsp1109I GCAGC 4 cut(s) 101, 242, 310, 442
MaeI CTAG 3 cut(s) 93, 312, 564
MaeIII GTNAC 1 cut(s) 241
MboII GAAGA 4 cut(s) 27, 46, 400, 532
MfeI CAATTG 1 cut(s) 557
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 3 cut(s) 219, 514, 557
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 8 cut(s) 19, 184, 277, 324, 369, 463, 525, 607
MseI TTAA 5 cut(s) 129, 237, 281, 396, 585
MslI CAYNNNNRTG 2 cut(s) 275, 297
MunI CAATTG 1 cut(s) 557
MwoI GCNNNNNNNGC 2 cut(s) 75, 230
NcoI CCATGG 1 cut(s) 298
NlaIII CATG 3 cut(s) 302, 307, 331
NlaIV GGNNCC 2 cut(s) 6, 475
NmuCI GTSAC 1 cut(s) 241
NspV TTCGAA 1 cut(s) 386
PaqCI CACCTGC 1 cut(s) 485
PflFI GACNNNGTC 1 cut(s) 338
PkrI GCNGC 4 cut(s) 91, 232, 325, 457
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PspN4I GGNNCC 2 cut(s) 6, 475
PsyI GACNNNGTC 1 cut(s) 338
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 2 cut(s) 275, 297
SaqAI TTAA 5 cut(s) 129, 237, 281, 396, 585
SatI GCNGC 4 cut(s) 90, 231, 324, 456
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 1 cut(s) 365
SduI GDGCHC 1 cut(s) 476
SetI ASST 8 cut(s) 94, 102, 155, 269, 372, 380, 423, 479
SfuI TTCGAA 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 275, 297
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 3 cut(s) 219, 514, 557
SspMI CTAG 3 cut(s) 93, 312, 564
StyI CCWWGG 2 cut(s) 298, 573
TaaI ACNGT 2 cut(s) 348, 526
TaqI TCGA 2 cut(s) 342, 386
TasI AATT 3 cut(s) 219, 514, 557
TatI WGTACW 1 cut(s) 46
Tru1I TTAA 5 cut(s) 129, 237, 281, 396, 585
Tru9I TTAA 5 cut(s) 129, 237, 281, 396, 585
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 4 cut(s) 89, 230, 323, 455
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 3 cut(s) 115, 320, 593
TspGWI ACGGA 1 cut(s) 479
Tth111I GACNNNGTC 1 cut(s) 338
XapI RAATTY 1 cut(s) 514
XspI CTAG 3 cut(s) 93, 312, 564
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.