MD07G1255400.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004281
Physical Location & Seq
Reverse (-)
32260788 .. 32261387
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1255400.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGCATCATCATCTTTTGTCACATCTTTTCTCTTAACACTTTCTTTTACTTGCTGCATATTCTCTTCACCCTCAGTAGCCAGAAACGATGCACAAAAAACCAACAATGAATTCATGAAAACATCTTGCAGTGCAACAACCTACCCAAAGCTTTGCCTTGCTTCTCTTTCAAGCCATGCAGGTGCAATCCAAACAAGCCCTAAACTTATGGCATGCGCAGCCCTTAACGTGACGCTTGCCTCTGCCAAATCAACTTCTACCATGATGTCAAAGCTCTCCCACAGCCATGGAATGGAGCCTAAAGAAGTCGGAGCTATGAGGGACTGTGTCGAAGAGTTAAGTGATTCGGTCTATGAGCTTAAGAGGTCTATAGCTGAGATGGGACGCTTTAAAAGCTCTGATTTTCAGCTTATGATAAGTGATGTACAAACATGGGCCAGTGCGGCTTTGACGGATGAGACCACCTGCTCTGATGGGTTTGAGGAAAATGGGATGAACGGGAAGTTGAAGACTATTGTGGGGGGCAGGATTGTCAAGATTGCACAATTGACTAGCAATGCTTTGGCTTTGATCAACATATACGCCTCAGTTCATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

200

Amino Acids

21.31

Weight (kDa)

6.42

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 190 4.7e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 170, 473
Acc16I TGCGCA 1 cut(s) 217
Acc36I ACCTGC 2 cut(s) 170, 473
AccB7I CCANNNNNTGG 1 cut(s) 292
AciI CCGC 1 cut(s) 443
AcsI RAATTY 1 cut(s) 110
AfaI GTAC 1 cut(s) 426
AfiI CCNNNNNNNGG 1 cut(s) 292
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 171, 508
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 7 cut(s) 151, 274, 314, 358, 374, 396, 409
AluI AGCT 7 cut(s) 151, 274, 314, 358, 374, 396, 409
Alw26I GTCTC 1 cut(s) 452
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 54, 218
ApoI RAATTY 1 cut(s) 110
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 515
BbvI GCAGC 2 cut(s) 41, 230
BccI CCATC 2 cut(s) 373, 467
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 552
BfmI CTRYAG 1 cut(s) 369
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 2 cut(s) 170, 473
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 3 cut(s) 55, 219, 444
BlsI GCNGC 3 cut(s) 56, 220, 445
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 297
BmsI GCATC 2 cut(s) 14, 79
BpiI GAAGAC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 460, 498
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 3 cut(s) 87, 366, 600
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 41, 230
BshFI GGCC 1 cut(s) 437
BslFI GGGAC 2 cut(s) 335, 396
BslI CCNNNNNNNGG 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 335, 396
BsnI GGCC 1 cut(s) 437
Bso31I GGTCTC 1 cut(s) 452
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 1 cut(s) 570
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 1 cut(s) 443
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 3 cut(s) 86, 367, 599
BspHI TCATGA 2 cut(s) 114, 592
BspLI GGNNCC 1 cut(s) 297
BspMI ACCTGC 2 cut(s) 170, 473
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 562
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 2 cut(s) 70, 327
BstAFI CTTAAG 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 214, 237
BstDEI CTNAG 3 cut(s) 73, 375, 586
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 2 cut(s) 460, 498
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 1 cut(s) 573
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 570
BstMWI GCNNNNNNNGC 3 cut(s) 218, 393, 443
BstNSI RCATGY 1 cut(s) 216
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 41, 230
BstV2I GAAGAC 1 cut(s) 515
BstXI CCANNNNNNTGG 1 cut(s) 287
BsuRI GGCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 2 cut(s) 460, 498
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 445
BveI ACCTGC 2 cut(s) 170, 473
Cac8I GCNNGC 2 cut(s) 214, 237
CciI TCATGA 2 cut(s) 114, 592
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 435
CseI GACGC 2 cut(s) 241, 393
Csp6I GTAC 1 cut(s) 425
CviAII CATG 7 cut(s) 115, 176, 213, 262, 287, 432, 593
CviQI GTAC 1 cut(s) 425
DdeI CTNAG 3 cut(s) 73, 375, 586
DpnI GATC 1 cut(s) 572
DpnII GATC 1 cut(s) 570
DraI TTTAAA 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 70, 327
EarI CTCTTC 2 cut(s) 70, 327
Eco130I CCWWGG 1 cut(s) 286
Eco31I GGTCTC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 7 cut(s) 118, 179, 216, 265, 290, 435, 596
FaqI GGGAC 2 cut(s) 335, 396
FatI CATG 7 cut(s) 114, 175, 212, 261, 286, 431, 592
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 3 cut(s) 55, 219, 444
FokI GGATG 2 cut(s) 467, 505
Fsp4HI GCNGC 3 cut(s) 55, 219, 444
FspBI CTAG 1 cut(s) 552
FspI TGCGCA 1 cut(s) 217
GlaI GCGC 1 cut(s) 217
GluI GCNGC 3 cut(s) 55, 219, 444
HaeIII GGCC 1 cut(s) 437
HgaI GACGC 2 cut(s) 241, 393
HhaI GCGC 1 cut(s) 218
Hin1II CATG 7 cut(s) 118, 179, 216, 265, 290, 435, 596
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 1 cut(s) 344
HphI GGTGA 1 cut(s) 60
Hpy188I TCNGA 3 cut(s) 311, 400, 472
Hpy188III TCNNGA 3 cut(s) 115, 535, 593
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 7 cut(s) 57, 92, 129, 134, 179, 185, 542
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 393, 443
HpyF3I CTNAG 3 cut(s) 73, 375, 586
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 7 cut(s) 118, 179, 216, 265, 290, 435, 596
HspAI GCGC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 1 cut(s) 570
LmnI GCTCC 2 cut(s) 295, 311
LpnPI CCDG 5 cut(s) 94, 165, 451, 478, 511
Lsp1109I GCAGC 2 cut(s) 41, 230
LweI GCATC 2 cut(s) 14, 79
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 19, 229
MalI GATC 1 cut(s) 572
MboI GATC 1 cut(s) 570
MboII GAAGA 3 cut(s) 57, 344, 520
MfeI CAATTG 1 cut(s) 545
MluCI AATT 2 cut(s) 110, 545
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 6 cut(s) 82, 250, 312, 357, 475, 595
MseI TTAA 6 cut(s) 35, 225, 338, 360, 390, 598
MslI CAYNNNNRTG 2 cut(s) 180, 285
MspCI CTTAAG 1 cut(s) 359
MunI CAATTG 1 cut(s) 545
MwoI GCNNNNNNNGC 3 cut(s) 218, 393, 443
NcoI CCATGG 1 cut(s) 286
NdeII GATC 1 cut(s) 570
NlaIII CATG 7 cut(s) 118, 179, 216, 265, 290, 435, 596
NlaIV GGNNCC 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 19, 229
NsbI TGCGCA 1 cut(s) 217
NspI RCATGY 1 cut(s) 216
PaeI GCATGC 1 cut(s) 216
PagI TCATGA 2 cut(s) 114, 592
PaqCI CACCTGC 2 cut(s) 170, 473
PfeI GAWTC 1 cut(s) 344
PflFI GACNNNGTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 292
PkrI GCNGC 3 cut(s) 56, 220, 445
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 435
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 1 cut(s) 426
RsaNI GTAC 1 cut(s) 425
RseI CAYNNNNRTG 2 cut(s) 180, 285
SaqAI TTAA 6 cut(s) 35, 225, 338, 360, 390, 598
SatI GCNGC 3 cut(s) 55, 219, 444
Sau3AI GATC 1 cut(s) 570
Sau96I GGNCC 1 cut(s) 435
SfaNI GCATC 2 cut(s) 14, 79
SfcI CTRYAG 1 cut(s) 369
SmiMI CAYNNNNRTG 2 cut(s) 180, 285
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SphI GCATGC 1 cut(s) 216
Sse9I AATT 2 cut(s) 110, 545
SsiI CCGC 1 cut(s) 443
SspMI CTAG 1 cut(s) 552
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 330
TaqII GACCGA 1 cut(s) 337
TasI AATT 2 cut(s) 110, 545
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 6 cut(s) 35, 225, 338, 360, 390, 598
Tru9I TTAA 6 cut(s) 35, 225, 338, 360, 390, 598
TscAI CASTG 2 cut(s) 136, 445
TseFI GTSAC 2 cut(s) 19, 229
TseI GCWGC 2 cut(s) 54, 218
Tsp45I GTSAC 2 cut(s) 19, 229
TspDTI ATGAA 5 cut(s) 103, 123, 131, 509, 581
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 2 cut(s) 136, 445
Tth111I GACNNNGTC 1 cut(s) 326
Van91I CCANNNNNTGG 1 cut(s) 292
Vha464I CTTAAG 1 cut(s) 359
XapI RAATTY 1 cut(s) 110
XceI RCATGY 1 cut(s) 216
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.